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Introduction: Urinary tract infection as one of the most common bacterial infections is known as a second cause of infection. However, sensitivity to a various health hygiene pads in children and Easy Life in elderly as well as poor sanitation can make people susceptible to infections of the urinary tract. For this reason, antibacterial textiles used in the preparation of health and medical textiles can deal with infections especially urinary tract infections effectively. Material and methods: First, the 550 clinical isolates were collected from several hospitals and laboratories in Shahreza city, among which, 100 bacterial isolates were positive and resistant to several antibiotics. Then the synthesized zinc oxide nanoparticles were established by using sol-gel method and under ultrasonic irradiation on linen bandages. Antibacterial activity of nano-fibers bacteria samples, based on zinc oxide by disk diffusion method in agar were assessed. Results and Conclusion: According to the results of nanofibers of zinc oxide have more antibacterial properties against gram-positive bacteria, causing urinary tract infections compared with negatives ones. It is hoped that the fibers introduced in this study in the future after the tests (in-situ) will be able to provide clothes for patients and personnel, gloves and surgical masks, and most importantly suture yarns, bandages, wound dressing and sanitary napkins should be used.