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BACKGROUND: Lettuce holds a prominent position in the year‐round supply of vegetables, offering a rich array of health‐beneficial substances, such as dietary fiber, phenolic compounds, lactucopicrin and lactucin. As such, its flavor has garnered increasing attention. Balancing the enhancement of beneficial compounds with the reduction of undesirable taste is a key focus of scientific research. To investigate short‐term management to improve the nutritional quality and flavor of lettuce, combinations of different light intensities (200, 500 and 800 μm ol m−2 s−1) and temperatures (10 and 22 °C) were applied separately to 'Lollo Rosso' and 'Little Butter Lettuce' for 7 days before harvest. RESULTS: The results obtained showed that increasing light intensity at low temperatures decreased nitrate content and increased soluble sugar, soluble protein, anthocyanin and phenolic compound content. In the case of lettuce flavor, the bitterness‐related metabolites such as lactucin and lactucopicrin were reduced with high light intensity at a low temperature of 10 °C. With this combination, the fructose and glucose contents increased, significantly improving lettuce flavor. CONCLUSION: Higher light intensity combined with low temperature for 7 days before harvest effectively improved the nutritional quality and flavor of lettuce, suggesting its great potential for use in horticultural practices. © 2024 Society of Chemical Industry. [ABSTRACT FROM AUTHOR]

In peri-urban areas, use of industrial wastewater for irrigation is a common practice. Industrial wastewater contains cadmium, chromium, lead, nickel, and other elements that deteriorate food quality and affect human health. Biochar has been proven to remediate heavy metal contaminated soil by reducing their mobility and bioavailability. A pot experiment was conducted to evaluate the efficiency of different levels of biochar on spinach growth with low heavy metal concentration and to minimize associated health issues. The experiment lasted two months and the treatments: Control (tap water), untreated and treated industrial wastewater and both in combination with biochar (0.5% and 1%) were applied in completely randomized design. Findings suggested that treated industrial wastewater with 1% biochar resulted in maximum plant height, shoot weight, chlorophyll contents (SPAD value), photosynthetic and transpiration rate. Biochar significantly reduced heavy metal mobility in soil due to its porous structure, high pH, higher CEC, and variety of surface functional groups. The cumulative hazard index (HI), hazard quotient, cancer risk, and total cancer risk (TCR) were calculated using method provided by US-EPA for each metal. All treatments had HI values of < 1, however applying 1% biochar significantly reduced the HI values to 2.00E-01 and 2.88E-01 in adults and children, respectively. TCR for all treatments was < 1, while treated industrial wastewater and biochar (1%) has significantly reduced to 1.55E-02 and 1.91E-03 for adults and children, respectively. Thus, it was determined that irrigation with industrial effluents caused toxicity in vegetables, which had a negative impact on human health. Biochar effectively mitigated metal toxicity in both soil and spinach plants that resulted in reduced health/cancer risk. [ABSTRACT FROM AUTHOR]

The current study aims to analyze heavy metals in leafy vegetables from the Musi River basin, which flows through Hyderabad, Telangana, India and is contaminated by domestic sewage, battery wastes, sewage sludge and industrial wastes. An atomic absorption spectrophotometer (AAS) was used to analyze the leaf samples for the presence of the heavy metals Cd, Pb, Zn, Fe, Mn and Cu. The effects of heavy metal contamination were examined in the soil and water of the Musi River, as well as in three green vegetables: Amaranth (Amaranthus viridis), Roselle (Hibiscus sabdariffa) and Malabar Spinach (Basella alba). The leafy vegetable Amaranth has the highest metal load (333.56 mg kg-1), followed by Malabar Spinach (328.17 mg kg-1) and Roselle (311.73 mg kg-1). [ABSTRACT FROM AUTHOR]

Introduction: The demand and consumption of vegetables are significantly increasing worldwide, which has resulted in urban farming on anthropogenic sites. This study assessed the concentrations of some selected heavy metals in lettuce and spring onion in line with the WHO/FAO required standard and its implications on human health. Methods: The study was carried out in Kumasi, within moist semi-deciduous forest vegetation, Ghana. The digested samples were analyzed for heavy metals (Cu, Cr, Fe, Mg, Ni, and Zn) using atomic absorption spectrophotometer (AAS Model AA 400p). Analysis of variance was used to test the level of significance at α =.05. Results: The study found mean concentrations of chromium and iron in lettuce and spring onion to be below detection level (BDL) in all study sites based on WHO/FAO permissible level. Also, while copper in lettuce was BDL at all the sites, there were higher mean concentration of copper in spring onion at BSGS (131.5 ± 0.31 mg/kg) and BSG (120.8 ± 0.01 mg/kg). The mean concentration of nickel in lettuce (137.15 ± 0.0231) and spring onion (173.55 ± 0.02 mg/kg) at BSGS were higher than WHO/FAO permissible level. Mean concentration of zinc in both lettuce and spring onion were higher than WHO/FAO permissible level in all the study sites, except zinc in spring onion at KT. The ANOVA test statistics showed no significant difference among the concentrations of heavy metals in all sites, except zinc in lettuce and nickel in spring onion. The study found cancer risk factor for nickel, which exceeded the benchmark of 1 × 10−6 for both lettuce and spring onion, indicating that long-term consumption could increase the risk of cancer in consumers. Conclusion: The study's findings call for strict regulation and regular monitoring of heavy metals in vegetables cultivated at anthropogenic sites in urban areas to ensure food safety and consumer health. Plain language summary: The study assessed the concentrations of some selected heavy metals in lettuce and spring onion in line with the WHO/FAO required standard and its implications on human health. Why was the study done? The WHO recommends consuming at least 400 g fruits and vegetables each day to reap health and nutritional benefits. This has resulted in increased advocacy for vegetable consumption and production. However, farmlands have become very scare due to proliferation of illegal mining activities in Ghana, making urban anthropogenic sites the idea sites for farming. Meanwhile, these sites can have adverse effect on human health since it may contain heavy metals in the soil used for planting and this could be easily absorbed into the food chain, particularly for leafy vegetables such as lettuce and spring onion. What did the research do? The research team selected samples of lettuce and spring onion for laboratory analysis. The digested samples were analyzed for heavy metals; copper (Cu), chromium (Cr), iron (Fe), magnesium (Mg), nickel (Ni) and zinc (Zn); using atomic absorption spectrophotometer (AAS Model AA 400p) so that we can determine the presence of heavy metals in the leafy vegetables. What did the research find? The study found permissible level of chromium and iron in both lettuce and spring onion in all the study sites. Also, while copper in lettuce was within the permissible limit at all the sites, there were higher concentration of copper in spring onion at BSGS and BSG. Nickel concentration in lettuce and spring onion at BSGS were higher than WHO/FAO permissible level, however, in AH and KT, they were less than the permissible limit. There was high concentration of zinc in both lettuce and spring onion in all the study sites, except zinc in spring onion at KT. The ANOVA test statistics showed a significant difference among the concentrations of zinc in lettuce and nickel in spring onion. [ABSTRACT FROM AUTHOR]

AbstractSalt stress, which impairs plant growth and induces oxidative stress, presents a major challenge in agriculture. In response, biochar has emerged as a promising soil amendment, capable of enhancing plants’ stress tolerance mechanisms. This study investigated the impact of rice husk biochar (RBC), derived from rice husks, on enhancing salt stress tolerance in lettuce plants grown under greenhouse conditions. Molecular and chemical characterization of the biochar samples was performed using Scanning Electron Microscopy (SEM) and Raman spectroscopy. Salinity reduced the fresh and dry weights of the plants from 25.0 g plant−1 and 1.83 g plant−1 to 20.4 g plant−1 and 1.42 g plant−1, respectively. The application of RBC recovered the growth decline caused by salinity, increasing the fresh and dry weights of the plants to 28.1 g plant−1 and 1.83 g plant−1, respectively. While the concentrations of sodium and chloride in plants decreased with RBC application, potassium and silicon concentrations significantly increased. As a result of salt stress, hydrogen peroxide levels (H2O2) increased in plant tissues, and a decrease in H2O2 levels was observed with an increase in superoxide dismutase, catalase, and ascorbate peroxidase activities. Consequently, RBC proved to be effective in enhancing salt tolerance in lettuce plants. In conclusion, while RBC demonstrates significant potential for enhancing salt tolerance, its successful application depends on several factors such as biochar dosage, application method, soil type, and plant species, underscoring the need for further research to elucidate the mechanisms at play and optimize its use for sustainable soil management in saline conditions. [ABSTRACT FROM AUTHOR]

The Sénoufo, a people from the north of Côte d'Ivoire, are renowned for their consumption of leafy vegetables, particularly in the little-known 'TCHONRON' sauce. The aim of this study is to draw up a list of the leafy vegetables used in the preparation of this dish, in order to enhance their value and contribute to the sustainable management of these plant genetic resources. To this end, an ethnobotanical survey was carried out among 640 Senufo indigenous people, using a questionnaire. A total of 40 leafy vegetable species belonging to 28 genera and 20 families were identified. In fact, the most represented families are Solanaceae, Amaranthaceae and Asteraceae. The majority of these species are herbs (58%). Of these species, 25% are introduced (exotic); they are used as substitutes for wild leafy vegetables by the population in times of scarcity. On the other hand, introduced species contribute to the diversity of leafy vegetables used in the preparation of "TCHONRON" sauce. In addition, the leafy vegetables used to prepare this local product are dominated by Guinean-Congolese and Sudanese-Zambezian (GC-SZ) species, representing 55% of all species inventoried. This study reveals that the leafy vegetables used to prepare the "TCHONRON" sauce are diversified, and that the consumption of this sauce, a product of the Senoufo region, is not only cultural, but also contributes to the prevention of several diseases. [ABSTRACT FROM AUTHOR]

The nitrate and nitrite content of four leafy vegetables (parsley, garden cress, coriander, and dill) from different regions of Tehran province, Iran (north, south, east, and west) were determined by spectrophotometer method at 538 nm. The results indicated that the dill had the highest nitrate concentration (402.944 mg kg-1), while the coriander had the highest nitrite concentration (0.672 mg kg-1), and these values were significantly higher than other vegetables (P<0.05). Garden cress had the lowest nitrate and nitrite concentrations, 288.556 and 0.378 mg kg-1, respectively. The estimated daily intake (EDI) and target hazard quotient (THQ) of both nitrate and nitrite were below the standard threshold. The EDI values for nitrate were 0.2216, 0.1980, 0.1746, and 0.1587 (mg kg-1 body weight/day) for dill, coriander, parsley, and garden cress, respectively, indicating that the health risk associated with consuming these vegetables was lower than the standard limit (3.7 mg kg-1 body weight /day). The THQ results for nitrate and nitrite samples revealed that they were all lower than 1, implying that the risk level is acceptable for chronic risk health exposure to leafy vegetables. In conclusion, our results showed that the studied vegetables were safe to consumption based on nitrate and nitrite concentrations. [ABSTRACT FROM AUTHOR]

Soil fertility is declining in low-input agriculture due to insufficient fertilizer application by small-scale farmers. On the other hand, concerns are rising regarding the environmental pollution of both air and water in high-input agriculture due to the excessive use of N fertilizers in short growing seasons for vegetable crops, which is directly linked to the health of human beings and environmental safety. This study aimed to determine genotypic differences in the Nitrogen Use Efficiency (NUE) levels of different leafy vegetable species (Arugula, Spinach, Cress, Parsley, and Dill) grown hydroponically under two different N rates, low N (0.3 mM) and high N (3.0 mM), and to identify the plant traits that are contributing to NUE. A nutrient solution experiment was conducted between March and April 2024 by using an aerated Deep-Water Culture (DWC) technique in a fully automated climate room with a completely randomized block design (CRBD) with three replications for five weeks. The results indicated that shoot growth, as well as root morphological and leaf physiological responses, was significantly (p < 0.001) affected by genotype, the N rate, and genotype–N rate interactions. Shoot growth in some vegetable species (Arugula, Spinach, and Cress) was significantly higher under a low N than a high N rate, illustrating that they have a great capability for NUE under low N stress conditions. Similar results were also recorded for the root growth of the N-efficient species under low N rates. The NUE levels of these species were closely associated with leaf physiological (leaf area, leaf chlorophyll index (SPAD), photosynthesis, and total leaf chlorophyll (a + b) and carotenoids) and root morphological (root length, root volume, and average root diameter) characteristics. These plant traits could be useful indicators for the selection and breeding of 'N-efficient' leafy vegetable species for sustainable low-input agriculture systems in the future. However, further investigation should be carried out at the field level to confirm their commercial production viability. [ABSTRACT FROM AUTHOR]

Leafy vegetables are very healthy for human beings having high contents of phyto- and physico-chemicals and are low in fat and calories. Mountainous regions worldwide have diverse agroclimatic conditions conducive for the cultivation of different leafy vegetables. Present research study assessed six leafy vegetables for their composition at fifteen different agroecological sites. Fresh leaves of six leafy vegetables, i.e. alfalfa, water clover, spinach, leaf mustard, pennywort, and fenugreek, were collected from the mountainous region. Procured leaf samples were brought to analytical laboratory and were subjected to physicochemical and phytochemical analysis, i.e. moisture content, pH, TSS, ascorbic acid, carotenoids, phenolic content, flavonoids, and antioxidant potential. Among all the sampling sites and vegetables, a significant variation was observed for the physicochemical and phytochemical parameters. In samples procured from different agroecological sites, the better retention of quality parameters was observed among the leaf sample collected from fifteen different agroecological sites. The highest values were recorded for moisture content in spinach at site 1, for pH in leaf mustard at sites 4, 5 and 6, for TSS in spinach at sites 6 and 9, for ascorbic acid in alfalfa at site 6, for total phenolics in spinach at site 5, for carotenoid in leaf mustard at site 5, for flavonoids in fenugreek at site 5, and for antioxidant in spinach at site 6. It can be concluded that mountainous regions are rich in agroecological conditions suitable for producing leafy vegetables carrying healthful constituents for the consumption by human beings.

BACKGROUND: The first step in the contamination of leafy vegetables by human pathogens is their attachment to the leaf surface. The success of this is influenced strongly by the physical and chemical characteristics of the surface itself (number and size of stomata, presence of trichomes and veins, epicuticular waxes, hydrophobicity, etc.). This study evaluated the attachment of Salmonella enterica to 30 baby‐leaf salads and tested whether the differences found among them were related to the following leaf traits: hydrophobicity, roughness, and epicuticular waxes. RESULTS: Differences in susceptibility to contamination by S. enterica were found between the 30 baby‐leaf salads investigated. The lowest attachment was found in wild lettuce (Lactuca serriola L.) and lamb's lettuce 'Trophy F1' (Valerianella locusta [L.] Laterr.), with values of 1.63 ± 0.39 Log(CFU/cm2) and 1.79 ± 0.54 Log(CFU/cm2), respectively. Attachment was correlated with hydrophobicity (measured as contact angle) (r = −0.39) and epicuticular waxes (r = −0.81) but not with roughness (r = 0.24). The most important wax components for attachment were alcohols and, in particular, the three‐dimensional (3D) wax crystals of C26 alcohol, but fatty acids probably also had a role. Both these compounds increased hydrophobicity. The presence of thymol, whose antimicrobial properties are well known, was found in lamb's lettuce. CONCLUSIONS: The findings of this study can help to predict and control the attachment and contamination of leafy salads by enterobacteria. They also provide useful information for breeding programs aiming to develop cultivars that are less susceptible to human pathogens, enhancing the food safety of vegetables. © 2024 The Author(s). Journal of the Science of Food and Agriculture published by John Wiley & Sons Ltd on behalf of Society of Chemical Industry. [ABSTRACT FROM AUTHOR]

Gibberellin (GA₃) is a growth hormone that is essential in plant physiological processes. At present, gibberellins are employed in commerce to enhance the physical traits and yield of various vegetable, horticultural, ornamental, and medicinal crops. This research aimed to study the effects of gibberellin application on the morphological characteristics and yield of lettuce plants. The study was arranged in a Completely Randomized Design (CRD) with six treatments of gibberellins concentration with a hydroponic system, namely: G0 = control, G1 = 20 ppm, G2 = 40 ppm, G3 = 60 ppm, G4 = 80 ppm, and G5 = 100 ppm, and each treatment consisted of four replications. This research was conducted in May–July 2023 at the Kemuning Greenhouse, Belu Regency, East Nusa Tenggara. GA₃s were sprayed twice at 2 weeks after sowing and 1 week after transplanting. The results showed that the highest GA₃ concentration (100 ppm) had a negative impact on plant morphology and yield, producing the smallest number of leaves, too long stem, pale green leaves, and the lowest fresh weight. Meanwhile, GA₃ concentration of 40 ppm resulted in the highest number of leaves. However, this increase is not directly proportional to the fresh weight of the leaves. GA₃ concentration of 40 ppm resulted in the best and most efficient yield of leaves and fresh weight, making it highly recommended.

Abstract Background Food-associated antibiotic-resistant bacteria can cause infections that may critically impact human health. The objectives of this study were to determine the microbial contamination level of green leafy vegetables and their antibiotic resistance pattern. Methods Sixty-three samples of leafy vegetables were collected from Dammam Central Fruit and Vegetables Market from January to June 2023. The vegetables included lettuce (Lactuca sativa), parsley (Petroselinum crispum), and watercress (Nasturtium officinale). Samples were tested by standard microbiological techniques for identification and antibiotic susceptibility testing. Result Eight types of bacteria belonging to six different genera were detected. Enterobacteriaceae family was represented by four genera: Klebsiella, Proteus, Morganella, and Enterobacter. The other two genera were Pseudomonas and Aeromonas. Enterobacter cloacae was the most abundant organism, followed by Pseudomonas putida and Aeromonas sobria. On the other hand, Morganella morganii, Aeromonas hydrophila, and Proteus mirabilis were the least abundant. The three vegetable types had different levels of bacterial contamination. All isolated organisms were sensitive to penicillin, cephalosporin, aminoglycoside, and fluoroquinolone. However, Klebsiella oxytoca, M. morganii, and K. pneumonia showed resistance to ampicillin. A. hydrophila, Morganella morganii, and E. cloacae showed resistance to amoxicillin. M. morganii and E. cloacae were found to be resistant to cefalotin. Moreover, A. hydrophila, M. morganii, and E. cloacae were resistant to cefoxitin. Again, A. hydrophila was found to be resistant to imipenem. Only M. morganii was resistant to Ciprofloxacin. Two isolates, P. mirabilis and M. morganii were resistant to tigecycline. Another two, M. morganii and P. mirabilis were resistant to Nitrofurantoin. Only M. morganii was found to be resistant to trimethoprim. Conclusion This study aligns with the broad consensus in the literature about the significance of bacterial contamination in vegetables and the public health implications. The unique focus on antibiotic resistance patterns adds an essential dimension to the existing body of knowledge.

Background: Food-associated antibiotic-resistant bacteria can cause infections that may critically impact human health. The objectives of this study were to determine the microbial contamination level of green leafy vegetables and their antibiotic resistance pattern. Methods: Sixty-three samples of leafy vegetables were collected from Dammam Central Fruit and Vegetables Market from January to June 2023. The vegetables included lettuce (Lactuca sativa), parsley (Petroselinum crispum), and watercress (Nasturtium officinale). Samples were tested by standard microbiological techniques for identification and antibiotic susceptibility testing. Result: Eight types of bacteria belonging to six different genera were detected. Enterobacteriaceae family was represented by four genera: Klebsiella, Proteus, Morganella, and Enterobacter. The other two genera were Pseudomonas and Aeromonas. Enterobacter cloacae was the most abundant organism, followed by Pseudomonas putida and Aeromonas sobria. On the other hand, Morganella morganii, Aeromonas hydrophila, and Proteus mirabilis were the least abundant. The three vegetable types had different levels of bacterial contamination. All isolated organisms were sensitive to penicillin, cephalosporin, aminoglycoside, and fluoroquinolone. However, Klebsiella oxytoca, M. morganii, and K. pneumonia showed resistance to ampicillin. A. hydrophila, Morganella morganii, and E. cloacae showed resistance to amoxicillin. M. morganii and E. cloacae were found to be resistant to cefalotin. Moreover, A. hydrophila, M. morganii, and E. cloacae were resistant to cefoxitin. Again, A. hydrophila was found to be resistant to imipenem. Only M. morganii was resistant to Ciprofloxacin. Two isolates, P. mirabilis and M. morganii were resistant to tigecycline. Another two, M. morganii and P. mirabilis were resistant to Nitrofurantoin. Only M. morganii was found to be resistant to trimethoprim. Conclusion: This study aligns with the broad consensus in the literature about the significance of bacterial contamination in vegetables and the public health implications. The unique focus on antibiotic resistance patterns adds an essential dimension to the existing body of knowledge. [ABSTRACT FROM AUTHOR]

Gibberellin (GA3) is a growth hormone that is essential in plant physiological processes. At present, gibberellins are employed in commerce to enhance the physical traits and yield of various vegetable, horticultural, ornamental, and medicinal crops. This research aimed to study the effects of gibberellin application on the morphological characteristics and yield of lettuce plants. The study was arranged in a Completely Randomized Design (CRD) with six treatments of gibberellins concentration with a hydroponic system, namely: G0 = control, G1 = 20 ppm, G2 = 40 ppm, G3 = 60 ppm, G4 = 80 ppm, and G5 = 100 ppm, and each treatment consisted of four replications. This research was conducted in May-July 2023 at the Kemuning Greenhouse, Belu Regency, East Nusa Tenggara. GA3s were sprayed twice at 2 weeks after sowing and 1 week after transplanting. The results showed that the highest GA3 concentration (100 ppm) had a negative impact on plant morphology and yield, producing the smallest number of leaves, too long stem, pale green leaves, and the lowest fresh weight. Meanwhile, GA3 concentration of 40 ppm resulted in the highest number of leaves. However, this increase is not directly proportional to the fresh weight of the leaves. GA3 concentration of 40 ppm resulted in the best and most efficient yield of leaves and fresh weight, making it highly recommended. [ABSTRACT FROM AUTHOR]

In the U.S., baby spinach is mostly produced in Arizona (AZ) and California (CA). Characterizing the impact of growing region on the bacterial quality of baby spinach can inform quality management practices in industry. Between December 2021 and December 2022, baby spinach was sampled after harvest and packaging for microbiological testing, including shelf-life testing of packaged samples that were stored at 4°C. Samples were tested to (i) determine bacterial concentration, and (ii) obtain and identify bacterial isolates. Packaged samples from the Salinas, CA, area (n = 13), compared to those from the Yuma, AZ, area (n = 9), had a significantly higher bacterial concentration, on average, by 0.78 log10 CFU/g (P < 0.01, based on aerobic, mesophilic plate count data) or 0.67 log10 CFU/g (P < 0.01, based on psychrotolerant plate count data); the bacterial concentrations of harvest samples from the Yuma and Salinas areas were not significantly different. Our data also support that an increase in preharvest temperature is significantly associated with an increase in the bacterial concentration on harvested and packaged spinach. A Fisher's exact test and linear discriminant analysis (effect size), respectively, demonstrated that (i) the genera of 2,186 bacterial isolates were associated (P < 0.01) with growing region and (ii) Pseudomonas spp. and Exiguobacterium spp. were enriched in spinach from the Yuma and Salinas areas, respectively. Our findings provide preliminary evidence that growing region and preharvest temperature may impact the bacterial quality of spinach and thus could inform more targeted strategies to manage produce quality. [ABSTRACT FROM AUTHOR]

Simple Summary: The impact of various soil disinfestation methods applied to soil was evaluated in a commercial field that has been repeatedly used for lettuce cultivation for two successive years. The populations of four soilborne pathogens, evaluated via RT-qPCR before and after the implementation of the specific methods, showed that all the evaluated methods significantly reduced the populations of the specific soilborne pathogens. More specifically, soil solarization was observed to be the most effective method. Amplicon sequencing analysis of 16S rRNA-encoding genes showed that the soil bacterial microbiomes were influenced by the implementation of the various soil treatments. Lettuce is the most cultivated leafy vegetable in Greece; however, due to the adopted intensive cropping system, its cultivation is susceptible to many soilborne pathogens that cause significant yield and quality losses. In the current study, the impact of various soil disinfestation methods such as solarization, chemical disinfestation, and application of a biofungicide were evaluated in a commercial field that has been repeatedly used for lettuce cultivation. The populations of soilborne pathogens Rhizoctonia solani, Pythium ultimum, Fusarium oxysporum, and Fusarium equiseti were measured via qPCR before and after the implementation of the specific disinfestation methods. Although all the tested methods significantly reduced the population of the four soilborne pathogens, soil solarization was the most effective one. In addition, solarization reduced the number of lettuce plants affected by the pathogens R. solani and F. equiseti, and at the same time, significantly influenced the growth of lettuce plants. Amplicon sequence analysis of 16S rRNA-encoding genes used to study the soil bacterial community structure showed that Firmicutes, Proteobacteria, and Actinobacteria were the predominant bacterial phyla in soil samples. In general, solarization had positive effects on Firmicutes and negative effects on Proteobacteria and Actinobacteria; soil fumigation with dazomet increased the relative abundance of Firmicutes and Proteobacteria and reduced the corresponding values of Actinobacteria; and biofungicide had no significant effects on the three predominant bacterial phyla. The bacterial community composition and structure varied after the application of the soil disinfestation treatments since they imposed changes in the α- and β-diversity levels. The results of this study are expected to contribute towards implementing the most effective control method against the most common soilborne pathogens in intensively cultivated fields, such as those cultivated with leafy vegetables. [ABSTRACT FROM AUTHOR]

Heavy metal contamination in leafy vegetables poses significant health risks, highlighting the urgent need for stringent monitoring and intervention measures to ensure food safety and mitigate potential adverse effects on public health. This study investigates the levels of heavy metals, including cadmium (Cd), chromium (Cr), nickel (Ni), lead (Pb), zinc (Zn), and copper (Cu), in locally grown and commercially available leafy vegetables, comparing them to the safety limits established by WHO/FAO. The results revealed that levels of Cd, Cr, Ni, and Pb in the vegetables exceeded WHO/FAO limits, while Zn and Cu remained within permissible bounds. Marketed vegetables exhibited higher metal concentrations than those from nearby farms. For Cu (0.114–0.289 mg/kg) and Zn (0.005–0.574 mg/kg), the daily intake of metals (DIM) was below the dietary intake (DI) and upper limit (UL). Cd's DIM (0.031–0.062 mg/kg) remained below the UL but exceeded the DI. Marketed kale and mint surpassed both DI and UL limits for Ni, while local produce only exceeded the DI. All vegetables had DIM below the DI, except for mint and kale. For Pb, every vegetable exceeded DI limits, with market samples contributing significantly. Cr's DIM ranged from 0.028 to 1.335 mg/kg, for which no set maximum daily intake exists. The health risk index (HRI) values for Zn, Cd, Cu, Ni, and Pb suggested potential health risks associated with leafy greens, while Cr's HRI was below 1. The study underscores the need for stringent monitoring and intervention measures to mitigate the health risks posed by heavy metal contamination in leafy vegetables. These findings suggest that consuming these leafy greens may put consumers at considerable risk for health problems related to Cd, Cu, Ni, Pb, and Zn exposure. [ABSTRACT FROM AUTHOR]

Over two billion people worldwide suffer from micronutrient deficiencies. Biofortifying vegetables can enhance micronutrient intake through the diet. This study assessed the biofortification of indoor-grown baby-leaf lettuce using aeroponics. Four experiments, two each, were conducted by adding different concentrations of Zn (from 10 to 450 µM) or Cu (from 3 to 250 µM) into a nutrient solution. A fifth experiment was conducted by simultaneously adding to the nutrient solution the optimal concentration of I (5 µM) and Se (13 µM), chosen on the basis of previous works, and the optimal concentration of Zn (250 µM) and Cu (150 µM), chosen on the basis of the results obtained in the first four experiments. Leaf biomass, mineral concentrations, chlorophylls, carotenoids, phenols, flavonoids, nitrates, and antioxidant capacity were measured 21 days after transplanting. Higher concentrations of Cu, Zn, I, or Se in the nutrient solution led to an increase in their concentrations in lettuce leaves, without affecting the growth or leaf quality of lettuce plants. The simultaneous application of I with the other elements induced a higher accumulation in leaves compared to when I is applied alone. One hundred grams of lettuce leaves biofortified with Se, I, Cu, and Zn would provide the 6.1%, 35.3%, and 263.0% of Adequate Intake for Cu, Se, and I, respectively, and 4.5% of Population Reference Intake for Zn. Our results suggest that simultaneously biofortifying baby-leaf lettuce with these four minerals is a practical and convenient way to integrate these micronutrients into the diet without reducing the yield or quality of lettuce. [ABSTRACT FROM AUTHOR]

The use of biostimulants in agriculture has been rapidly increasing in recent years, because of their positive effects on crop yield, product quality, and tolerance to abiotic stresses. In the present study the efficacy of multiple applications of a plant-derived biostimulant, obtained from the aqueous maceration of borage (Borago officinalis, L.) flowers on two lettuce cultivars, namely a green (Lactuca sativa L. cv. Expertise RZ) and red (Lactuca sativa L. cv. Codex RZ) Salanova® was evaluated. The treatment was applied at 10 mL L−1 as foliar spray three times, once a week starting from two weeks after transplant. Control plants were treated with water. Non-destructive measurements (pigments, leaf nitrogen index, chlorophyll a fluorescence) were taken during plant growth after at each treatment application. At the end of the experiment, destructive analyses were performed to assess qualitative traits. The research work was focused on the evaluation of physiological parameter changes during plant growth, and on primary and secondary metabolism. Foliar applications did not affect the accumulation of total sugars (4.56 mg g−1 in Expertise, 3.5 mg g−1 in Codex) in either cultivar. However, the lettuce head weight was negatively affected by the extract application in red cultivar (-10 g/plant), while no changes were observed in the green lettuce. The nitrogen-flavonol index (NFI) increased after the third application of borage extract in green cultivar (+67 %), suggesting an improvement of nitrogen nutrition status or a reduced stress condition. A different response resulted in term of maximum quantum efficiency of PSII (FV/FM), performance index (PI), nitrate, and anthocyanin accumulation in leaves. The FV/FM ratio significantly increased in green cultivar after the first application (from 0.80 to 0.84) and at harvest (from 0.79 to 0.84). The PI showed a slight but not significant increase at the same time points. On the contrary, the PI was significantly higher in red cultivar after the third application (+9.4 %). Interestingly, the borage extract induced a significant decrease of nitrate accumulation in lettuce leaves of the red cultivar (from 4149.7 to 2711.6 mg/kg, −34 %). At the same time a positive variation of anthocyanin content was observed in red lettuce (+24.7 %). The application of biostimulant products might improve the quality of some lettuce varieties as regards the accumulation of metabolites useful for the plant to overcome stress conditions and fundamental in human healthy diet, increasing the leaf concentration of Ca, Na, and Mg.

IntroductionUrea is one of the nitrogen chemical fertilizers for vegetable production in soil. But it is seldom used in soilless cultures. Leafy vegetables such as Lettuce (Lactuca sativa L.) contain high levels of nitrate and attempts have been made to reduce the nitrate concentration in this crop for human consumption. Using reduced forms of nitrogen, i.e. urea, is one of the applied strategies for reducing nitrate accumulation in lettuce. Little information is available concerning urea as a source of nitrogen for production of leafy vegetables such as lettuce in soilless culture. This experiment was conducted to investigate the effect of different ratios of urea:nitrate in nutrient solution on the growth indices, yield and nitrate accumulation of red French lettuce (Lactuca sativa L. cv. Lolla Rossa) in soilless culture. Materials and MethodsA hydroponic experiment using completely randomized design was carried out with seven ratios of urea:nitrate in nutrient solution and four replications in the research greenhouse of Shahrekord University. Urea:nitrate ratios in nutrient solution were: 0:100, 10:90, 20:80, 30:70, 40:60, 50:50 and 60:40. Lettuce seedlings were grown in 2 L plastic pots (one plant per pot) containing mixture of cocopeat + perlite at the ratio of 2:1 (v/v) and were manually fertigated with nutrient solutions on a daily basis. Four weeks after transplanting, lettuce plants were harvested and fresh weights of shoot and root were determined. Plant growth indices including of plant height, plant diameter, leaf length, leaf width, leaf number, leaf greenness index and leaf brix level were measured. After measuring the growth indices, the leaves were grouped separately according to leaf numbers 1-10=outer leaves, >11= inner leaves. The samples were dried in an oven at 60 °C and were ground. Nitrate concentrations in samples were determined calorimetrically using a spectrophotometer at a wavelength of 410 nm. Analysis of variance was performed using SAS software version 9.4. Means comparison was conducted using least significant difference test at 0.05 probability level. Results and DiscussionThe results indicated that application of different ratios of urea to nitrate in nutrient solution had not significant effect on the lettuce growth indices including of plant diameter, leaf length, leaf width, leaf number, leaf greenness index and leaf brix level in comparison with 0:100 of urea:nitrate ratio. Also, root and shoot fresh weights were not affected by urea:nitrate ratio in nutrient solution. The greatest quantity of shoot fresh weight (141 g per plant) was obtained with a 50:50 urea:nitrate ratio. However, this was not significantly different from the shoot fresh weight (125 g per plant) observed when urea was not included in the nutrient solution. Shoot nitrogen concentration (except for plants nourished with a 50:50 urea:nitrate ratio) was not affected by increasing the urea:nitrate ratio in the nutrient solution. The results revealed that application of urea in nutrient solution effectively provided the nitrogen requirement of lettuce. This indicates that lettuce plants can efficiently hydrolyze urea and use it efficiently as a nitrogen source. Application of urea in the nutrient solution led to significant decrease in the nitrate concentration of lettuce root (P< 0.05). Moreover, increasing urea:nitrate ratio in nutrient solution resulted in significant decrease of the nitrate concentration of outer leaves, inner leaves and all leaves of lettuce (P< 0.01). The highest and lowest nitrate concentration in inner, outer and all leaves of lettuce were obtained in plants nourished with 0:100 and 50:50 urea:nitrate ratio in nutrient solution, respectively. Application of urea:nitrate ratio of 50:50 led to the meaningful decrease of nitrate concentration in root (43%), outer leaves (41%), inner leaves (44%) and all leaves (43%) of lettuce in comparison with 0:100 of urea:nitrate ratio. Urea had a repressive effect on nitrate influx and decreased its uptake by plants. Also, after urea uptake by plant root, it is first degraded by cytosolic ureases and then ammonium is incorporated via the GS-GOGAT (Glutamine Synthetase- Glutamine α-OxoGlutarate Amino Transferaze) cycle. Therefore, application of urea in nutrient solution can lead to the reduction of nitrate accumulation in plants. ConclusionBased on the shoot fresh weight and nitrate concentration in lettuce leaves, replacing 50% of nitrate in nutrient solution with urea is recommended for red French lettuce production in hydroponic culture under the conditions of the present study. Compared to other nitrogen fertilizers, urea has a lower price and its application in nutrient solution is useful in reducing production costs.

Mekelle is one of the Ethiopian cities suitable for urban and peri-urban agriculture for cultivating leafy vegetables using wastewater. The consumption of unprocessed and processed leafy vegetables is also very high in the city. Wastewater samples collected from four experimental sites (ESs) in Hadnet district of the city had higher concentrations in 4 (i.e., Cd, Cr, Cu, and Mn) of the 10 heavy metals tested than the permissible limit established by pertinent standards. Spring water samples collected from another site called Kallamino, designated as a comparison site, also had higher concentrations in 4 (i.e., Al, As, Cd, and Cu) of the 10 heavy metals tested. However, the leafy vegetables grown in the least contaminated ES had higher concentrations in 6-7 of the 10 heavy metals tested. The lettuce and spinach samples had elevated concentrations of As and Al, respectively. The wastewater used to irrigate vegetable farms in Hadnet district is not safe enough. More importantly, the soils of the farms might have accumulated far more heavy metals. The cultivated lettuce, spinach, and cauliflower are highly contaminated. Thus, the use of wastewater for irrigating urban and peri-urban farms needs to be regulated. HIGHLIGHTS Tests of wastewaters used in urban agriculture for heavy metal contents.; Tests of three leafy vegetables irrigated with wastewater for heavy metal contents.; Contamination of the three leafy vegetables with high level of 6 to 7 heavy metals tested.;

Purpose: The effectiveness of the evaporative clay pot coolers was studied to preserve the postharvest quality of leafy vegetables, including kankun (Ipomoea aquatica), gotukola (Centella asiatica), lettuce (Lactuca sativa) and thampala (Amaranthus spp.). Research method: The bundles of leafy vegetables weighed about 200 g and were stored for seven days under room temperature storage, refrigerated storage, and clay pot cooler storage. Findings: The average temperatures were 27.7 °C, 7.1 °C, and 25.6 °C, and relative humidity values were 76.1%, 58.2%, and 93.6% in room temperature storage, refrigerated storage, and clay pot cooler, respectively. The average cooling efficiency of clay pot coolers was 66.7%. The physiological weight losses of leafy vegetables were significantly reduced during clay pot cooler storage. The chlorophyll content, soluble solids content, color changes, and visual quality of leafy vegetables were significantly maintained in clay pot cooler storage compared to room temperature storage. Research limitations: The main limitation of this study was the seepage of water into the inner pot of the clay pot cooler which enhanced the decay of leafy vegetables. This was successfully controlled by avoiding overwatering the sand that was used as the lining material of the clay pot cooler. Originality/Value: The clay pot cooler is one of the alternative low-cost storage methods to preserve the quality of leafy vegetables during storage.

IntroductionUrea is one of the nitrogen chemical fertilizers for vegetable production in soil. But it is seldom used in soilless cultures. Leafy vegetables such as Lettuce (Lactuca sativa L.) contain high levels of nitrate and attempts have been made to reduce the nitrate concentration in this crop for human consumption. Using reduced forms of nitrogen, i.e. urea, is one of the applied strategies for reducing nitrate accumulation in lettuce. Little information is available concerning urea as a source of nitrogen for production of leafy vegetables such as lettuce in soilless culture. This exfaiment was conducted to investigate the effect of different ratios of urea:nitrate in nutrient solution on the growth indices, yield and nitrate accumulation of red French lettuce (Lactuca sativa L. cv. Lolla Rossa) in soilless culture. Materials and MethodsA hydroponic exfaiment using completely randomized design was carried out with seven ratios of urea:nitrate in nutrient solution and four replications in the research greenhouse of Shahrekord University. Urea:nitrate ratios in nutrient solution were: 0:100, 10:90, 20:80, 30:70, 40:60, 50:50 and 60:40. Lettuce seedlings were grown in 2 L plastic pots (one plant fa pot) containing mixture of cocopeat + falite at the ratio of 2:1 (v/v) and were manually fertigated with nutrient solutions on a daily basis. Four weeks after transplanting, lettuce plants were harvested and fresh weights of shoot and root were determined. Plant growth indices including of plant height, plant diameter, leaf lenth, leaf width, leaf number, leaf greenness index and leaf brix level were measured. After measuring the growth indices, the leaves were grouped separately according to leaf numbers 1-10=outer leaves, >11= inner leaves. The samples were dried in an oven at 60 °C and were ground. Nitrate concentrations in samples were determined calorimetrically using a spectrophotometer at a wavelenth of 410 nm. Analysis of variance was faformed using SAS software version 9.4. Means comparison was conducted using least significant difference test at 0.05 probability level. Results and DiscussionThe results indicated that application of different ratios of urea to nitrate in nutrient solution had not significant effect on the lettuce growth indices including of plant diameter, leaf lenth, leaf width, leaf number, leaf greenness index and leaf brix level in comparison with 0:100 of urea:nitrate ratio. Also, root and shoot fresh weights were not affected by urea:nitrate ratio in nutrient solution. The greatest quantity of shoot fresh weight (141 g fa plant) was obtained with a 50:50 urea:nitrate ratio. However, this was not significantly different from the shoot fresh weight (125 g fa plant) observed when urea was not included in the nutrient solution. Shoot nitrogen concentration (except for plants nourished with a 50:50 urea:nitrate ratio) was not affected by increasing the urea:nitrate ratio in the nutrient solution. The results revealed that application of urea in nutrient solution effectively provided the nitrogen requirement of lettuce. This indicates that lettuce plants can efficiently hydrolyze urea and use it efficiently as a nitrogen source. Application of urea in the nutrient solution led to significant decrease in the nitrate concentration of lettuce root (P< 0.05). Moreover, increasing urea:nitrate ratio in nutrient solution resulted in significant decrease of the nitrate concentration of outer leaves, inner leaves and all leaves of lettuce (P< 0.01). The highest and lowest nitrate concentration in inner, outer and all leaves of lettuce were obtained in plants nourished with 0:100 and 50:50 urea:nitrate ratio in nutrient solution, respectively. Application of urea:nitrate ratio of 50:50 led to the meaningful decrease of nitrate concentration in root (43%), outer leaves (41%), inner leaves (44%) and all leaves (43%) of lettuce in comparison with 0:100 of urea:nitrate ratio. Urea had a repressive effect on nitrate influx and decreased its uptake by plants. Also, after urea uptake by plant root, it is first degraded by cytosolic ureases and then ammonium is incorporated via the GS-GOGAT (Glutamine Synthetase- Glutamine α-OxoGlutarate Amino Transferaze) cycle. Therefore, application of urea in nutrient solution can lead to the reduction of nitrate accumulation in plants. ConclusionBased on the shoot fresh weight and nitrate concentration in lettuce leaves, replacing 50% of nitrate in nutrient solution with urea is recommended for red French lettuce production in hydroponic culture under the conditions of the present study. Compared to other nitrogen fertilizers, urea has a lower price and its application in nutrient solution is useful in reducing production costs. [ABSTRACT FROM AUTHOR]

The PlantEye multispectral scanner is an optoelectrical sensor automatically applied to a mechatronic platform that allows the non-destructive, accurate, and high-throughput detection of morphological and physiological plant parameters. In this study, we describe how the advanced phenotyping platform precisely assesses changes in plant architecture and growth parameters of wild rocket salad (Diplotaxis tenuifolia L. [DC.]) under drought stress conditions. Four different irrigation supply levels from moderate to severe, required to keep 100, 70, 50, and 30% of the water-holding capacity, were adopted. Growth rate and plant architecture were recorded through the digital measure of biomass, leaf area, Canopy Light Penetration Depth, five convex hull traits, plant height, Surface Angle Average, and Voxel Volume Total. Vegetation color assessments included hue, lightness, and saturation. Vegetation and senescence indices were calculated from canopy reflectance in the red (620–645 nm), green (530–540 nm), blue (peak wavelength 460–485 nm), near-infrared (820–850 nm), and 3D laser (940 nm) ranges. The temperature, relative humidity, and solar radiation of the environment were also recorded. Overall, morphological parameters, color, multispectral data, and vegetation indices provided over 7200 data points through daily scans over three weeks of cultivation. Although a general decrease in growth parameters with increasing stress severity was observed, plants were able to maintain the same morpho-physiological performances as the control during the early growth stages, keeping both 70% and 50% of the total water-holding capacity. Among indices, the Normalized Differential Vegetation Index (NDVI) contributed the most to the differentiation between different stress levels during the cultivation cycle. Across the 3 weeks of growth, statistically significant differences were observed for all traits except for the Saturation Average. Comparisons with respect to the control highlighted the strong impact of drought stress on morphological plant traits. This study provided meaningful insights into the health status of wild rocket salad under increasing drought stress. [ABSTRACT FROM AUTHOR]

This study focused on the osmotic dehydration (OD) of ready-to-eat spinach leaves combined with the pulsed electric field (PEF) pre-treatment. Untreated and PEF-treated (0.6 kV/cm, 0–200 pulses) spinach leaves were osmotically dehydrated at room temperature for up to 120 min. The application of PEF (0.6 kV/20 pulses) prior to OD (60% glycerol, 25 °C, 60 min) lowered water activity (aw = 0.891) while achieving satisfactory product acceptability (total sensory hedonic scoring of 8). During the storage of the product (at 4, 8, 12, and 20 °C for up to 30 d), a significant reduction in total microbial count evolution was observed (9.7 logCFU/g for the untreated samples vs. 5.1 logCFU/g for the PEF-OD-treated samples after 13 d of storage at 4 °C). The selection of these PEF and OD treatment conditions enabled the extension of the product shelf life by up to 33 d under chilled storage. Osmotically treated spinach could find application in ready-to-eat salad products with an extended shelf life, which is currently not possible due to the high perishability of the specific plant tissue. [ABSTRACT FROM AUTHOR]

Vernonia amygdalina is an African traditional leafy vegetable which is a source of income for many households involved in its production in West and Central Africa. The species possesses several medicinal and nutritional properties. Despite its importance, there is limited information on farmers' access to seed, seed production practices, seed trade, and related constraints. This research aimed at investigating farmers' seed access and management practices. A non-probabilistic snowball method was used to sample and interview 164 bitter leaf seed sector actors from urban and peri-urban areas in Benin. Results indicated that vegetable farming is mainly practiced by men and it was the main occupation for 94.47% of the farmers interviewed. A significant association was found between the location and farmers' experience (pvalue = 0.01358). A diversity of Vernonia amygdalina morphotypes were found. Sixty-three percent of the farmers surveyed use seeds as planting material, 29% use purchased seedlings and only 7% use stem cuttings for Vernonia amygdalina production. Most of the seeds used (74%) were from farmer-saved seeds, harvested from isolated plants during production. The main constraints to V. amygdalina production reported by farmers were the unavailability of seeds (41% of respondents), low germination percentage (18%) and low seed longevity (12%). Findings from this study can inform solutions to improve farmers' access to high-quality seeds and enhance V. amygdalina vegetable production. [ABSTRACT FROM AUTHOR]

In recent years, much effort has been devoted to understanding the response of plants to different light properties, largely due to advances in the light-emitting diode (LED) industry. This work studied the effect of different light intensities and qualities on yield or quality of indoor hydroponic spinach (Spinacia oleracea L.). Two trials were carried out at two different times. The intensity assay was carried out with the same type of light (AP673L, Valoya Ltd., Helsinki, Finland) at different luminous intensities (150, 290, and 430 µmol m−2 s−1). In the second trial, four different luminance spectra (Valoya Ltd., Helsinki, Finland) were used (NS12, AP67, AP673L, G2). Then, the fresh and dry weight, nutritional status, and concentration of primary metabolites were determined. Both lights parameters induced changes in vegetative performance and other physiological traits, as well as their quality and nutritional composition (minerals, organic acids, sugars, and amino acids). The increase in light intensity increased Fv'/Fv', fresh weight, leaf area, chlorophyll fluorescence parameters, and potassium concentration. The light intensity effectively controlled nitrate accumulation in an inverse relationship. The effect of the light spectrum on spinach characteristics was not clearly observed when multivariate statistics were applied to the data. No linear relationship was found between the different R/B ratios. This is perhaps due to commercial lights having a complex combination of wavelengths, in addition to the main R/B proportion. Within the overall results, 6 R/B presented the best results for the indoor cultivation of spinach. More studies are needed, since breeding for controlled environments shifts the focus of the desired crop attributes towards rapid growth and harvest quality instead of stress adaptability. [ABSTRACT FROM AUTHOR]

Sulfur (S) is an essential plant nutrient that regulates plant growth and metabolism. However, S is often absent from certain one-bag hydroponic fertilizers designed to provide a complete and balanced mixture of nutrients. We quantified the effects of S supplementation on the growth, morphology, and photosynthesis of lettuce grown in a deep-water culture hydroponic system. Two lettuce (Lactuca sativa) cultivars, green butterhead Rex and red oakleaf Rouxai, were grown using a prepackaged fertilizer specially formulated for reverse osmosis (RO) and other low-alkalinity water sources. The base nutrient solution was mixed using Jack's FeED 12-4-16 fertilizer and RO water at a nitrogen concentration of 100 mg.L21 (control). Three S supplementation treatments were implemented over a 4-week production period: 10 mg.L21 supplemental S (provided using MgSO4); 20 mg.L21 supplemental S (MgSO4); and a treatment using H2SO4 (instead of nitric acid) for pH adjustment. In both lettuce cultivars, shoot fresh and dry mass, total leaf area, leaf photosynthetic rate, total chlorophyll content, and leaf S concentration with all three S supplementation treatments increased significantly compared with those of the control. In contrast, the ratio of shoot dry mass to fresh mass, root dry mass, and percentage of root dry mass (i.e., root dry mass/total shoot and root mass) were significantly higher with the control treatment. Notably, 'Rouxai' lettuce grown in the control treatment had intense red coloration with a 216.6% to 288.9% increase in the anthocyanin index. There were no statistical differences in any of the growth and morphological parameters among the three S supplementation treatments. Overall, we observed significantly enhanced lettuce growth and photosynthetic performance with S supplementation, resulting in a 144.0% to 215.9% increase in shoot fresh mass in the two cultivars compared with the control. Thus, we recommend that at least 10 mg.L21 of S should be supplemented when growing lettuce hydroponically to ensure optimal plant growth, especially when S is absent or low in the fertilizer and water source. [ABSTRACT FROM AUTHOR]

A rapid analytical method for the simultaneous determination of 550 pesticide residues in vegetable samples was developed based on ultra-high performance liquid chromatography-tandem Q/Orbitrap high-resolution mass spectrometry (UPLC-Q/Orbitrap-HRMS). To investigate the risk of exposure to pesticide residues through vegetable consumption, 704 leafy vegetable samples from Shanghai were analysed for multiple residues using this method. A total of 54 pesticide residues were identified in these vegetable samples and 302 samples contained one or more pesticide residue. The levels of the detected pesticides did not pose a health risk in the long term and were acceptable according to the results of the chronic dietary risk assessment. Risk rankings displayed that most of the pesticides were low to medium risk. The findings of this study provide a reference for future pesticide monitoring programmes. [ABSTRACT FROM AUTHOR]

Purpose: The effectiveness of the evaporative clay pot coolers was studied to preserve the postharvest quality of leafy vegetables, including kankun (Ipomoea aquatica), gotukola (Centella asiatica), lettuce (Lactuca sativa) and thampala (Amaranthus spp.). Research method: The bundles of leafy vegetables weighed about 200 g and were stored for seven days under room temperature storage, refrigerated storage, and clay pot cooler storage. Findings: The average temperatures were 27.7 °C, 7.1 °C, and 25.6 °C, and relative humidity values were 76.1%, 58.2%, and 93.6% in room temperature storage, refrigerated storage, and clay pot cooler, respectively. The average cooling efficiency of clay pot coolers was 66.7%. The physiological weight losses of leafy vegetables were significantly reduced during clay pot cooler storage. The chlorophyll content, soluble solids content, color changes, and visual quality of leafy vegetables were significantly maintained in clay pot cooler storage compared to room temperature storage. Research limitations: The main limitation of this study was the seepage of water into the inner pot of the clay pot cooler which enhanced the decay of leafy vegetables. This was successfully controlled by avoiding overwatering the sand that was used as the lining material of the clay pot cooler. Originality/Value: The clay pot cooler is one of the alternative low-cost storage methods to preserve the quality of leafy vegetables during storage. [ABSTRACT FROM AUTHOR]

Common sowthistle (Sonchus oleraceus L.) and bristly oxtongue [Helminthotheca echioides (L.) Holub] are winter broad-leaved weeds that have gained interest for cultivation as leafy vegetables. The aim of this study was to examine the effects of frass from the yellow mealworm (Tenebrio molitor L.) on nutrient content in soil, growth parameters, and nutrient content in above-ground plant tissues of common sowthistle and bristly oxtongue. Thus, two pot experiments were carried out with 5 treatments [control, calcium ammonium nitrate (CAN) applied at a dose of 100 kg N ha−1, and insect frass applied at a rate of 3500 kg ha−1 (0.5 % w/w) 7000 kg ha−1 (1 % w/w), and 14,000 kg ha−1 (2 % w/w)]. Our results showed that the lowest values of growth parameters for both plant species were recorded in the control treatment. At the final rosette growth stage [e.g., 152 days after sowing (DAS)], the CAN treatment exhibited the highest values of rosette diameter and above-ground dry weight, followed by the highest rate of insect frass. Similarly, at 152 DAS the SPAD index values in the CAN treatment were 28.4–41.5 % higher compared to the control treatment in both species. Regarding root dry weight, the highest values were found in the CAN and insect frass 2 % treatments. In addition, the application of insect frass significantly enhanced soil fertility, with the highest levels of P and K recorded in the insect frass 2 % treatment. In contrast, the CAN treatment resulted in the highest NO3–N content in the soil (15.83 and 19.26 mg kg−1 in common sowthistle and bristly oxtongue, respectively). Moreover, both P and K content in the above-ground plant tissues had the highest values in the insect frass 2 % treatment, while the content of Mg, Mn, and Cu in plant tissues was not affected by the fertilization sources. Therefore, our findings indicate that insect frass can be an additional option in crop fertilization programs as it can improve both the soil fertility and growth of crops compared to conventional inorganic fertilizer sources. However, the effects of insect frass in mixtures with inorganic fertilizers needs to be taken into consideration in future studies.

Every year, approximately one-third of the total food produced for human consumption is lost or wasted due to various reasons. This level of wastage has substantial adverse impacts on the environment, economy, and society. Numerous studies have proposed various policies to address the issue of food waste, such as incorporating technology into existing supply chains. However, concerns about their effectiveness and unintended consequences have led researchers to emphasize market-based approaches for waste reduction. The present study was carried out to estimate waste and investigate the potential for developing different marketing channels as market-based approaches to reduce waste in the leafy vegetable supply chain in Kermanshah province. To achieve this purpose, a system dynamics modelling of the waste system in the leafy vegetable supply chain was developed by using the literature review and interviews with experts and stakeholders. The tool for collecting research data was a questionnaire. The statistical population of this study is two groups including 22 experts and 728 actors in the leafy vegetable supply chain. Based on the findings, around 31,000 tonnes (39%) of leafy vegetables are wasted annually across the supply chain. The research scenarios indicate that the establishment of processing industries will effectively decrease the overall waste of leafy vegetables from around 31,000 tons to approximately 20,000 tons annually. Therefore, government initiatives and policies in the field of leafy vegetable exchange in the study area must focus on supporting businesses associated with leafy vegetable processing industries and establishing infrastructure prerequisites for these industries.

This study assessed the effect of the steamed and boiling cooking methods on the vitamin content of six staple green vegetables in Nigeria. Vitamin levels in fresh, steamed and boiled leafy vegetables were determined using spectrophotometric and titrimetric methods. The results of vitamin analysis showed that fresh vegetable of Amaranthus hybridus contained the largest amount of ascorbic acid, thiamin and niacin (358.40 mg/100 g, 0.09 mg/100 g and 0.08 mg/100 g), while Talinum triangulare had the highest level of riboflavin (0.18 mg/100 g). The steamed Amaranthus hybridus contained the highest levels of ascorbic acid, thiamin and niacin (322.56 mg/100 g, 0.08 mg/100 g and 0.73 mg/100 g), while steamed leaves of Talinum triangulare had the highest quantity of riboflavin (0.16 mg/100 g). The boiled Amaranthus hybridus had the highest amount of ascorbic acid, thiamin and niacin (197.12 mg/100 g, 0.05 mg/100 g and 0.41 mg/100 g), whereas the boiled Talinum triangulare had the highest level of riboflavin (0.09 mg/100 g). The percentage of vitamin loss in steamed leafy vegetables varies from 9 to 11 %, 9 to 17 %, 9 to 13 % and 9 to 12 % in ascorbic acid, thiamin, riboflavin and niacin respectively. Almost 50 % of the essential vitamins were lost in boiled leafy vegetables. The results revealed that vegetables could be improved source of some important vitamins that can help humans meet their nutritional needs.

Soil fertility is declining in low-input agriculture due to insufficient fertilizer application by small-scale farmers. On the other hand, concerns are rising regarding the environmental pollution of both air and water in high-input agriculture due to the excessive use of N fertilizers in short growing seasons for vegetable crops, which is directly linked to the health of human beings and environmental safety. This study aimed to determine genotypic differences in the Nitrogen Use Efficiency (NUE) levels of different leafy vegetable species (Arugula, Spinach, Cress, Parsley, and Dill) grown hydroponically under two different N rates, low N (0.3 mM) and high N (3.0 mM), and to identify the plant traits that are contributing to NUE. A nutrient solution experiment was conducted between March and April 2024 by using an aerated Deep-Water Culture (DWC) technique in a fully automated climate room with a completely randomized block design (CRBD) with three replications for five weeks. The results indicated that shoot growth, as well as root morphological and leaf physiological responses, was significantly (p < 0.001) affected by genotype, the N rate, and genotype–N rate interactions. Shoot growth in some vegetable species (Arugula, Spinach, and Cress) was significantly higher under a low N than a high N rate, illustrating that they have a great capability for NUE under low N stress conditions. Similar results were also recorded for the root growth of the N-efficient species under low N rates. The NUE levels of these species were closely associated with leaf physiological (leaf area, leaf chlorophyll index (SPAD), photosynthesis, and total leaf chlorophyll (a + b) and carotenoids) and root morphological (root length, root volume, and average root diameter) characteristics. These plant traits could be useful indicators for the selection and breeding of ‘N-efficient’ leafy vegetable species for sustainable low-input agriculture systems in the future. However, further investigation should be carried out at the field level to confirm their commercial production viability.

Nickel (Ni) is a toxic metal that not only pollutes the environment but also causes harmful impacts on plant growth and human health. Therefore, it is crucial to assess the relationship between the phytoavailability of Ni in soil and its accumulation in edible and non-edible parts of vegetables. A pot experiment was conducted to investigate Ni uptake in three different leafy vegetables, spinach (Spinacia oleracea L.), lettuce (Lactuca sativa L.), and fenugreek (Trigonella foenum-graecum L.), grown in soil artificially contaminated with Ni at three different treatment levels (100 mg kg−1, 200 mg kg−1, and 300 mg kg−1). The potential dietary toxicity of these vegetables in humans was examined by using an in vitro digestion model. The lowest and highest chlorophyll contents were detected in lettuce at 300 mg kg−1 of Ni concentration and in control plants of spinach. Their values were 34.16 ± 3.01 (SPAD unit) and 53 ± 3.7673 (SPAD unit), respectively. Among the three vegetables, lettuce and spinach at 300 mg kg−1 exhibited the highest accumulation of Ni, with 43 mg kg−1 in edible parts and 182 mg kg−1 in non-edible parts. Furthermore, health risk index (HRI) values were found to be > 1 for lettuce and fenugreek at Ni concentrations of 200 and 300 mg kg−1 for both children and adults. The average bioaccessibility of Ni in lettuce, fenugreek, and spinach during the gastrointestinal phase was 32–23%, 24–10%, and 45–37%, respectively, at a Ni concentration of 300 mg kg−1. All three vegetables grown on Ni-contaminated soil may potentially contribute to food chain toxicity. The HRI values being > 1 suggest that these vegetables are unsafe for consumption. Monitoring of Ni concentration in leafy vegetables is essential to minimize human health risks associated with food chain contamination. [ABSTRACT FROM AUTHOR]

Biochar, as a soil amendment, can be applied to remediate heavy metal (HM) contaminated farmland. However, there is little research on the effect of tobacco biochar (TB) derived from tobacco waste on HM controlling in edible parts of vegetables. In this study, the impact of two TB levels on the plant growth, copper (Cu) and cadmium (Cd) accumulation in the edible parts of lettuce and chrysanthemum, and on Cu and Cd bioavailability of rhizosphere soil was investigated through in-situ field experiments. The results showed that TB has rich oxygen containing functional groups, high porosity, high nitrogen adsorption capacity. The addition of 5 t ha−1 and 10 t ha−1 TB significantly increased the shoot biomass of chrysanthemum, but had no effect on the growth of lettuce. Two levels of TB significantly increased the pH value, but decreased the available Cu and Cd concentrations of rhizosphere soil, thereby reducing the Cu and Cd accumulations in the edible parts of lettuce and chrysanthemum. The findings provided effective evidences that TB derived from tobacco waste is an efficient strategy for controlling Cu and Cd accumulation in the edible parts of vegetables to ensure agri-product safety production in HM-polluted farmland. [ABSTRACT FROM AUTHOR]

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Every year, approximately one-third of the total food produced for human consumption is lost or wasted due to various reasons. This level of wastage has substantial adverse impacts on the environment, economy, and society. Numerous studies have proposed various policies to address the issue of food waste, such as incorporating technology into existing supply chains. However, concerns about their effectiveness and unintended consequences have led researchers to emphasize market-based approaches for waste reduction. The present study was carried out to estimate waste and investigate the potential for developing different marketing channels as market-based approaches to reduce waste in the leafy vegetable supply chain in Kermanshah province. To achieve this purpose, a system dynamics modelling of the waste system in the leafy vegetable supply chain was developed by using the literature review and interviews with experts and stakeholders. The tool for collecting research data was a questionnaire. The statistical population of this study is two groups including 22 experts and 728 actors in the leafy vegetable supply chain. Based on the findings, around 31,000 tonnes (39%) of leafy vegetables are wasted annually across the supply chain. The research scenarios indicate that the establishment of processing industries will effectively decrease the overall waste of leafy vegetables from around 31,000 tons to approximately 20,000 tons annually. Therefore, government initiatives and policies in the field of leafy vegetable exchange in the study area must focus on supporting businesses associated with leafy vegetable processing industries and establishing infrastructure prerequisites for these industries. [ABSTRACT FROM AUTHOR]

[Objective] To explore the application of fermented mushroom residue and fermented corn cob in improving leaf vegetable seedling substrate. (Methods Lettuce and romaine lettuce were used as test materials. Using different volume rations of fermented mushroom residue and fermented corn cob mixed with compound substrate of turf and vermiculite mixture, and turf:vermiculite=7V:3V as control (CK), the effects of different substrate ratios on the physicochemical properties of the substrate as well as the growth and development of lettuce and romaine lettuce seedlings were studied. [Results] In the different ratios of compound substrates, the bulk density of T3 treatment was highest among all the treatments at 0.48 g/cm³. The total porosity, aeration porosity, total phosphorus content, available nitrogen content, pH value, and EC value of T6 treatment were highest among all the treatments, at 79.26%,29.60%,0.21 g/kg,1 564.76 mg/kg, 7.51, and 4.47 mS/cm, respectively. The total potassium content of T3 treatment was highest among all the treatments, at 6.44 g/kg. The effective phosphorus content of T7 treatment was highest among all the treatments, at 18.86 mg/kg. The available potassium content of T3 treatment was highest among all the treatments, at 380.68 mg/kg. The emergence rates of lettuce and romaine lettuce in the substrate of T8 treatment (21% fermented mushroom residue, 21% fermented corn cob, 28% turf, and 30% vermiculite) reached 95.59% and 95.96%, respectively. The plant height, stem diameter, single leaf area, and total dry weight of the seedlings were 8.92 cm, 2.08 mm, 20.13 cm²,93.70 mg, and 15.11 cm,3.50 mm,7.40 cm²,164.01 mg, respectively, all significantly higher than CK (P<0.05). The comprehensive evaluation coefficients of seedling growth indexes were 0.974 and 0.959, respectively, all higher than CK and other treatments. [Conclusion] The addition of fermented mushroom residue and fermented corn cob significantly improved the physicochemical properties of leafy vegetable seedling substrate. Equal volume of fermented mushroom residue and fermented corn cob mixture had significant promoting effects on the growth and development of lettuce and romaine lettuce seedlings, could partially replace turf in the seedling substrate of leafy vegetables. [ABSTRACT FROM AUTHOR]

The soil type in China are rich and diverse, and the occurrence forms of heavy metals in different regions are different. It is difficult to compare and evaluate the effectiveness of heavy metals in different regions and different types of soils. In order to screen and establish a method for evaluating the effectiveness of heavy metals in farmland soils in suitable areas, five typical farmland soils (yellow brown soil, yellow soil, red soil, lime soil and purple soil) with different properties in eastern Yunnan were selected. The extraction conditions (solid-liquid ratio, extractant pH value and oscillation time) of ABDTPA method were orthogonally combined by orthogonal test, the soil-crop Pb migration model was established to compare the goodness of fit with the total method, CaCl2 -DTPA method and AB-DTPA method. Pot experiments were carried out with Chinese cabbage and spinach as indicator plants to explore the correlation between different analysis methods and plant Pb accumulation ability, and then comprehensively evaluate the suitability of different methods.The results showed that the solidliquid ratio (g/mL) 1:3, pH 7.6 and shaking time 120 min had a great influence on the extraction amount of Pb; The optimized AB-DTPA method (solid-liquid ratio (g/mL) 1:3, pH 7.6, and oscillation time of 120 min) obtained through orthogonal experimental screening has the best effect on predicting the effective state extraction amount of Pb and the amount of Pb absorbed by crops, which can better predict the crop's ability to absorb Pb; The pot experiment showed that the available Pb content extracted by the optimized AB-DTPA method had a better correlation with the Pb content absorbed by Chinese cabbage and spinach ( R² = 0.898, R² = 0.752 ), with a Pb spiked recovery rate range of 99.5%-113.0% and high accuracy. Therefore, the optimized AB-DTPA method (Solid-liquid ratio 1:3, pH 7.6 and shaking time 120 min) is suitable for the extraction of Pb available state in Yunnan farmland soil. The method is feasible, universal and accurate. The research results provide a basis for the effect of regional farmland soil remediation technology and pollution risk assessment. [ABSTRACT FROM AUTHOR]

Field trials were executed at the University of Horticultural Sciences, Bagalkot, Karnataka during the kharif season of 2016 and 2017 to study the growth and productivity of ajwain and leafy vegetables under intercropping system including economic profitability of such cropping systems in Northern Karnataka. The experiments were carried out with thirteen treatments comprising sole ajwain and sole crops of leafy vegetables (fenugreek, coriander, dill and amaranthus) and various combinations of ajwain and leafy vegetables in 1:1 and 1:2 ratios adopting randomized complete block design with three replications. Results showed that the sole crop of ajwain exhibited superior growth and yield. However, in intercropping systems, the combination of ajwain and coriander at a 1:1 ratio demonstrated the highest growth and yield parameters. The sole crop of ajwain recorded the maximum plant height (84.64 and 84.55 cm), number of branches per plant (52.38 and 52.19), number of umbels per plant (166.54 and 164.93), number of umbellates per umbel (12.44 and 12.34), and seed yield (12.95, 12.88 q ha-1 during both the years, respectively). This performance was comparable to intercropping with ajwain + coriander (1:1). Sole crops of fenugreek, coriander, dill, and amaranthus yielded higher fresh market yields compared to different intercropping systems. Notably, growing ajwain as an intercrop with coriander at 1:1 ratio resulted in a significantly higher land equivalent ratio (1.61) and a higher benefit--cost ratio (2.99) followed by ajwain + fenugreek (1:1) and (2.98) respectively. [ABSTRACT FROM AUTHOR]

BACKGROUND: About 35–45% of the global population is affected by iodine deficiency. Iodine intake can be increased through the consumption of biofortified vegetables. Given the increasing interest in wild edible species of new leafy vegetables due to their high nutritional content, this study aimed to evaluate the suitability of Swiss chard (Beta vulgaris ssp. vulgaris var. cicla) and its wild ancestor sea beet (Beta vulgaris ssp. maritima) to be fortified with iodine. Plants were cultivated hydroponically in a nutrient solution enriched with four different concentrations of iodine (0, 0.5, 1.0, and 1.5 mg L−1), and the production and quality of baby leaves were determined. RESULTS: Sea beet accumulated more iodine than Swiss chard. In both subspecies, increasing the iodine concentration in the nutrient solution improved leaf quality as a result of greater antioxidant capacity – the ferric reducing ability of plasma (FRAP) index increased by 17% and 28%, at 0.5 and 1.5 mg L−1 iodine, respectively – the content of flavonoids (+31 and + 26%, at 1 and 1.5 mg L−1 of iodine, respectively), and the lower content of nitrate (−38% at 1.5 mg L−1 of iodine) and oxalate (−36% at 0.5 mg L−1 of iodine). In sea beet, however, iodine levels in the nutrient solution higher than 0.5 mg L−1 reduced crop yield significantly. CONCLUSIONS: Both subspecies were found to be suitable for producing iodine‐enriched baby leaves. The optimal iodine levels in the nutrient solution were 1.0 in Swiss chard and 0.5 mg L−1 in sea beet, as crop yield was not affected at these concentrations and leaves contained enough iodine to satisfy an adequate daily intake with a serving of 100 g. © 2023 Society of Chemical Industry. [ABSTRACT FROM AUTHOR]

Vegetables are a crucial component of every diet and a good source of vitamins and minerals. Many raw vegetables, such as salads, are consumed, and consuming raw vegetables contaminated with human and animal feces can lead to a variety of intestinal parasite diseases. The goal of this cross-sectional study was to investigate the incidence of contamination of green leafy vegetables with human intestinal parasites, as well as identify the most common parasite species and any seasonal variation in parasite abundance in green leafy vegetables found and sold for human consumption in various markets and stores in Jeddah. To the best of our knowledge, no research has been conducted on the prevalence of parasite-contaminated vegetables in Jeddah. From September 2020 to July 2021, 250 leafy vegetables (coriander, watercress, lettuce, parsley, leek, and green onion) were collected from various markets and stores in Jeddah. The vegetables were sorted, labelled, and evaluated in the laboratory after being completely washed with distilled water, examined for sediment, staining, and microscopically examined. Chromatographic immunoassay tests were also used to confirm parasite identification. Intestinal parasites were discovered in 35.2% of the vegetables (88/250). Coriander was the most contaminated (64.2%), while green onion was the least contaminated (21.9%). Blastocystis spp. was the most often discovered parasite (55.7%), followed by Strongyloides spp. larva (22.7%) and Entamoeba coli cysts (6.8%). Summer has the greatest percentage of parasites, followed by autumn, while winter has the fewest. There was no statistically significant relationship between the kind of vegetable, seasonal parasite prevalence in green vegetables, and parasite type.These findings highlight the importance of inspecting vegetables grown or imported from endemic countries for parasite contamination. In Jeddah, several fresh green vegetables were tainted with intestinal parasites. This demonstrates that those who consume raw vegetables regularly may be susceptible to parasitic illnesses. These findings highlight the need of monitoring vegetables grown or imported from parasitic-endemic areas. As a result, it is critical to establish strategies and control measures to limit the likelihood of parasites in food-borne diseases. [ABSTRACT FROM AUTHOR]

Early detection of pathogenic fungi in controlled environment areas can prevent major food production losses. Grey mould caused by Botrytis cinerea is often detected as an infection on lettuce. This paper explores the use of vegetation indices for early detection and monitoring of grey mould on lettuce under different lighting conditions in controlled environment chambers. The aim was focused on the potential of using vegetation indices for the early detection of grey mould and on evaluating their changes during disease development in lettuce grown under different lighting conditions. The experiment took place in controlled environment chambers, where day/night temperatures were 21 ± 2/17 ± 2 °C, a 16 h photoperiod was established, and relative humidity was 70 ± 10% under different lighting conditions: high-pressure sodium (HPS) and light-emitting diode (LED) lamps. Lettuces were inoculated by 7-day-old fungus Botrytis cinerea isolate at the BBCH 21. As a control, non-inoculated lettuces were grown under HPS and LEDs (non-inoculated). Then, the following were evaluated: Anthocyanin Reflectance Index 2 (ARI2); Carotenoid Reflectance Index 2 (CRI2); Structure Intensive Pigment Index (SIPI); Flavanol Reflectance Index (FRI); Greenness (G); Greenness 2 (G2); Redness (R); Blue (B); Blue Green Index 2 (BGI2); Browning Index 2 (BRI2); Lichtenthaler Index 1 (LIC1); Pigment Specific Simple Ratio (PSSRa and PSSRb); Gitelson and Merzlyak (GM1 and GM2); Zarco Tejada–Miller Index (ZMI); Normalized Difference Vegetation Index (NDVI); Simple Ratio (SR); Red-Eye Vegetation Stress Index (RVSI); Photochemical Reflectance Index (PRI); Photochemical Reflectance Index 515 (PRI515); Water Band Index (WBI); specific disease index for individual study (fD); Healthy Index (HI); Plant Senescence Reflectance (PSRI); Vogelmann Red Edge Index (VREI1); Red Edge Normalized Difference Vegetation Index (RENDVI); and Modified Red Edge Simple Ratio (MRESRI). Our results showed that the PSRI and fD vegetation indices significantly detected grey mould on lettuce grown under both lighting systems (HPS and LEDs) the day after inoculation. The results conclusively affirmed that NDVI, PSRI, HI, fD, WBI, RVSI, PRI, PRI515, CRI2, SIPI, chlorophyll index PSSRb, and coloration index B were identified as the best indicators for Botrytis cinerea infection on green-leaf lettuce (Lactuca sativa L. cv Little Gem) at the early stage of inoculated lettuce's antioxidative response against grey mould with a significant increase in chlorophyll indices. [ABSTRACT FROM AUTHOR]

Digital tools to predict produce shelf life have the potential to reduce food waste and improve consumer satisfaction. To address this need, we (i) performed an observational study on the microbial quality of baby spinach, (ii) completed growth experiments of bacteria that are representative of the baby spinach microbiota, and (iii) developed an initial simulation model of bacterial growth on baby spinach. Our observational data showed that the predominant genera found on baby spinach were Pseudomonas, Pantoea and Exiguobacterium. Rifampicin-resistant mutants (rifR mutants) of representative bacterial subtypes were subsequently generated to obtain strain-specific growth parameters on baby spinach. These experiments showed that: (i) it is difficult to select rifR mutants that do not have fitness costs affecting growth (9 of 15 rifR mutants showed substantial differences in growth, compared to their corresponding wild-type strain) and (ii) based on estimates from primary growth models, the mean (geometric) maximum population of rifR mutants on baby spinach (7.6 log10 CFU/g, at 6°C) appears lower than that of the spinach microbiota (9.6 log10 CFU/g, at 6°C), even if rifR mutants did not have substantial growth-related fitness costs. Thus, a simulation model, parameterized with the data obtained here as well as literature data on home refrigeration temperatures, underestimated bacterial growth on baby spinach. The root mean square error of the simulation’s output, compared against data from the observational study, was 1.11 log10 CFU/g. Sensitivity analysis was used to identify key parameters (e.g., strain maximum population) that impact the simulation model’s output, allowing for prioritization of future data collection to improve the simulation model. Overall, this study provides a roadmap for the development of models to predict bacterial growth on leafy vegetables with strain-specific parameters and suggests that additional data are required to improve these models.