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2 results on '"layer 6 neurons"'

1. Local Connections of Pyramidal Neurons to Parvalbumin-Producing Interneurons in Motor-Associated Cortical Areas of Mice.

Author

Kuramoto E, Tanaka YR, Hioki H, Goto T, and Kaneko T

Subjects
Animals, Interneurons physiology, Mice, Mice, Transgenic, Pyramidal Cells physiology, Dendrites physiology, Parvalbumins
Abstract

Parvalbumin (PV)-producing neurons are the largest subpopulation of cortical GABAergic interneurons, which mediate lateral, feedforward, and feedback inhibition in local circuits and modulate the activity of pyramidal neurons. Clarifying the specific connectivity between pyramidal and PV neurons is essential for understanding the role of PV neurons in local circuits. In the present study, we visualized somas and dendrites of PV neurons using transgenic mice in which PV neurons specifically express membrane-targeted GFP, and intracellularly labeled local axons of 26 pyramidal neurons in layers 2-6 in acute slices of the motor-associated cortex from transgenic mice. We mapped morphologically distribution of inputs from a pyramidal neuron to PV neurons based on contact sites (appositions) between the axons from an intracellularly filled pyramidal neuron and the dendrites of PV neurons. Layer 6 corticothalamic (CT)-like pyramidal neurons formed appositions to PV neurons at a significantly higher rate than other pyramidal neurons. The percentage of apposed varicosities to all the labeled varicosities of layer 6 CT-like neurons was 28%, and that of the other pyramidal neurons was 12-19%. Layer 6 CT-like neurons preferentially formed appositions with PV neurons in layers 5b-6, while other pyramidal neurons uniformly formed appositions with PV neurons in all layers. Furthermore, both layer 6 CT-like and corticocortical-like neurons more frequently formed compound appositions, where two or more appositions were located on a dendritic branch, than other pyramidal neurons. Layer 6 CT neurons may contribute to intracortical information processing through preferential connections with PV neurons in layers 5b-6., (Copyright © 2022 Kuramoto et al.)

Published
2022
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2. A Circuit for Integration of Head- and Visual-Motion Signals in Layer 6 of Mouse Primary Visual Cortex

Author

Lee Cossell, Troy W. Margrie, Sepiedeh Keshavarzi, Stephen C. Lenzi, Mateo Vélez-Fort, Charly V. Rousseau, Molly Strom, and Edward F. Bracey

Subjects
Male, 0301 basic medicine, Visual perception, genetic structures, Computer science, Nerve net, Motion Perception, Mice, Transgenic, Angular velocity, Visual system, Article, 2P imaging, mouse primary visual cortex, Mice, 03 medical and health sciences, 0302 clinical medicine, Retrosplenial cortex, medicine, Animals, Visual Pathways, head-velocity signals, Motion perception, Visual Cortex, Vestibular system, go/no go task, General Neuroscience, Bayesian approach, whole-cell patch clamp, layer 6 neurons, Mice, Inbred C57BL, 030104 developmental biology, medicine.anatomical_structure, Visual cortex, Neuropixels dense silicon probe, Head Movements, egocentric framework, Female, Nerve Net, Neuroscience, Photic Stimulation, 030217 neurology & neurosurgery
Abstract

Summary To interpret visual-motion events, the underlying computation must involve internal reference to the motion status of the observer’s head. We show here that layer 6 (L6) principal neurons in mouse primary visual cortex (V1) receive a diffuse, vestibular-mediated synaptic input that signals the angular velocity of horizontal rotation. Behavioral and theoretical experiments indicate that these inputs, distributed over a network of 100 L6 neurons, provide both a reliable estimate and, therefore, physiological separation of head-velocity signals. During head rotation in the presence of visual stimuli, L6 neurons exhibit postsynaptic responses that approximate the arithmetic sum of the vestibular and visual-motion response. Functional input mapping reveals that these internal motion signals arrive into L6 via a direct projection from the retrosplenial cortex. We therefore propose that visual-motion processing in V1 L6 is multisensory and contextually dependent on the motion status of the animal’s head., Highlights • Inputs onto V1 L6 neurons convey head-motion information • These L6 signals are widespread and provide a reliable estimate of angular velocity • In L6 neurons, vestibular and visual inputs sum during sensory processing • At least in part, V1 L6 head-motion signals are conveyed via an RSP-V1 pathway, V1 layer 6 neurons receive a widespread head-motion signal that is integrated with visual input during visual-motion processing. These V1 head-motion inputs project from the retrosplenial cortex, a multisensory area involved in spatial navigation and contextual processing.

Published
2018
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