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Langusei (Ficus minahassae (Teysm.et.Vr.) Miq) is one of the endemic flora of Sulawesi which existence is increasingly threatened. Attempts to maintain the existence of Ficus minahassae species by understanding the cultivation techniques of the Langusei species, especially regarding the appropriate germination techniques, need to be addressed. This study aims to determine the level of fruit maturity and the appropriate extraction treatment for the germination of Langusei seeds. The experimental design used in this study was a complete randomization design which is arranged in a factorial pattern. The first factor is the classification of the level of seed maturity based on fruit color categories: 1) Orange-brown fruit, 2) Orange-reddish fruit, 3) Red fruit, and 4) Red-black fruit, while the second factor is the fruit extraction treatment which consists of: 1) Extraction with dry wind treatment for 24 hours, 2) Extraction with drying treatment for 12 hours, and 3) Extraction with water treatment for 24 hours. The results showed that the fruit maturity level and the appropriate extraction treatment to produce optimal Langusei seed germination were in the (orange-brown and orange-reddish fruit) (mature fruits prior to ripening) which was soaked in water for 24 hours.

The langusei plant (Ficus minhassae (Teijsm. & De Vriese) Miq.) is widely believed to have numerous health benefits and is frequently processed into traditional medicine to treat a variety of diseases. This study aimed to investigate the potential inhibition of bioactive compounds from langusei leaves against SARS-CoV-2 using an in silico approach.The compounds docked to receptors were obtained from the PubChem site, while the receptors were obtained from the Protein Data Bank site. The docking process was carried out using Autodock Vina software. The best docking result was the lowest binding free energy value (kcal/mol). The ligands used in this study have a relatively high binding affinity, particularly the β-sitosterol with the receptor-binding domain, which has a binding affinity of -8.4 kcal/mol. Additionally, the phytyl fatty acid ester has a value of -7.3 kcal/mol when bound to the spike glycoprotein (closed state). According to the findings of this study, β-sitosterol and phytyl fatty acid esters had the highest binding affinity for several SARS-CoV-2 receptors. As a result, these two compounds found in langusei leaves have the potential to be developed as anti-SARS-CoV-2 drugs.