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Kvaliteta sjemena od presudnog je značaja za poljoprivrednu proizvodnju. Osnovni pokazatelji kvalitete sjemena su vlaga, masa 1000 sjemenki, čistoća, energija klijanja, klijavost i zdravstveno stanje. Energija klijanja i klijavost sjemena ispituju se prema točno propisanoj metodici ovisno o biljnoj vrsti. Za svaku biljnu vrstu propisana je podloga za naklijavanje, temperatura u procesu klijanja, broj dana potrebnih za očitavanje energije i klijavosti sjemena., te predtretman ako se radi o sjemenu koje ima izraženo svojstvo dormantnosti. Temperature ispitivanja klijavosti prilagođene su optimalnim temperaturama u vrijeme sjetve određene biljne vrste, no posljednjih godina zbog klimatskih prilika često se dogodi da su temperature u vrijeme sjetve zrnatih mahunarki osjetno niže u odnosu na višegodišnji prosjek. Cilj istraživanja je utvrditi utjecaj različitih temperatura na energiju klijanja i klijavost sjemena zrnatih mahunarki. Ispitivanje energije klijanja i klijavosti sjemena graška (Pisum sativum L.), stočnog graška jarog i ozimog (Pisum arvense L.), graha zrnaša visokog i niskog (Phaseolus vulgaris L.), graha mahunara visokog i niskog (Phaseolus vulgaris L.), boba (Vicia faba L.) i soje (Glycine max L.) provedeno je standardnom metodom u pijesku na tri temperature 15 °C, 20 °C i 25 °C. Propisana temperatura ispitivanja klijavosti kod svih vrsta je 25 °C, osim kod boba i graška 20 °C. Rezultati istraživanja pokazuju da je klijavost sjemena na temperaturi od 15 °C niska kod svih ispitivanih vrsta, osim kod boba i ozimog stočnog graška, dok je energija klijanja sjemena graha zrnaša, graha mahunara i soje 0 %. Temperatura od 20 °C negativno je utjecala na energiju klijanja i klijavost sjemena graha zrnaša, graha mahunara i jarog stočnog graška, dok je kod boba, ozimog stočnog graška i soje utjecaj temperature bio pozitivan na oba pokazatelja kvalitete sjemena. Sjeme graška, jarog stočnog graška, graha zrnaša i graha mahunara imalo je najvišu energiju klijanja i klijavost na temperaturi od 25 °C. Niske temperature u vrijeme nicanja mogu izazvati značajne štete na usjevima zrnatih mahunarki jer je kod većine vrsta energija klijanja 0 %, a klijavost sjemena vrlo niska., Seed quality is crucial for agricultural production. The basic indicators of seed quality are moisture, weight of 1000 seeds, purity, germination energy, germination and health. Germination energy and seed germination are tested according to a precisely prescribed methodology depending on the plant species. For each plant species, the substrate for germination is prescribed, the temperature in the process of germination, the number of days required to read the energy and germination of seeds, and pre-treatment if the seed has a pronounced dormant property. Germination testing temperatures are adjusted to optimal temperatures at the time of sowing a particular plant species, but in recent years due to climatic conditions it often happens that temperatures at the time when grain legumes are sown are significantly lower than the multi-year average. The aim of the research is to determine the influence of different temperatures on germination energy and germination of grain legume seeds. Testing of germination energy and germination of seeds of peas, fodder peas (spring and winter), grain beans (high and low), green beans (high and low), broad beans and soybeans was performed by the standard method in sand at three temperatures 15 °C, 20 °C and 25 °C. The prescribed germination testing temperature for all species is 25 °C, except for broad beans which is 20 °C. Research results show that seed germination at a temperature of 15 ° C is low in all tested species, except for spring peas and broad beans, while the germination energy of grain bean seeds, green beans and soybeans is 0%. The temperature of 20 °C had a negative effect on the germination energy and germination of seeds of grain beans, green beans and spring fodder peas, while in broad beans, winter fodder peas and soybeans the effect of temperature was positive on both seed quality indicators. Seeds of peas, grain beans and green beans had the highest germination energy and germination at a temperature o f 25 ° C. Low t temperatures a t t he time of germination can cause significant damage to grain legume crops because in most species the germination energy is 0% and the germination of seeds is very low.

The aim of the research was to evaluate the effect of year and seed hybrids on the most significant indicators of the seed quality of cucumber. Testing the quality of seeds of three different hybrids of cucumber: Centauro F1, Edona F1 and Solatio F1 was carried out during a five-year period (2018 - 2022). Analysis of germination energy, seed germination, abnormal seedlings and dead seeds cucumber showed highly significant differences (r

This study investigated changes in the germination rate and germination percentage of seeds obtained from closed pine cones (Pinus brutia Ten.) of different ages collected at different altitudes. The seeds used in the study were obtained from closed cones (3/control, 4, 5, 6, and 7 years old) in the lower (0-200 m) and higher (800-1000 m) altitude zones of the Mersin-Anamur region in a section from the sea to the interior. The seeds germinated in the dark at a constant temperature of 20 °C for 28 days. At the end of the germination test, a two-way analysis of variance was performed on the germination percentage data on the 10th, 14th, and 28th days, and the interactions between altitude and cone age were found to be significant (p = 0.05). As a result of the significant interaction between the two groups, the mean separation test (Tukey test) showed that the highest germination rate was 89.0% in the control group, and the lowest was 77.5% in the 7-year-old cones. In the higher altitude zone, the highest germination rate was 74.0% and the lowest was 71.0%, obtained from 5- and 7-year-old seeds, respectively. In general, germination values in the lower altitude zone were higher than those in the higher altitude zone. However, the decrease in germination values from the control to the 7-year-old seeds was greater in the lower altitude zone than in the higher altitude zone. The study found that altitude was more effective than cone age on the germination rate and percentage., U ovom istraživanju analizirane su promjene u stopi klijavosti i postotku klijavosti sjemena iz zatvorenih češera Pinus brutia Ten. različite starosti, sakupljenih na različitim nadmorskim visinama. Sjeme korišteno u istraživanju dobiveno je iz zatvorenih češera (3/kontrola, 4, 5, 6 i 7 godina starosti) u nižim (0-200 m) i višim (800-1000 m) visinskim zonama Mersin- Anamurske regije rasprostranjenih od mora prema unutrašnjosti. Sjeme je klijalo u mraku pri konstantnoj temperaturi od 20 °C 28 dana. Na kraju testa klijavosti provedena je dvostruka analiza varijance o postotku klijavosti 10., 14. i 28. dana, te je utvrđeno da su interakcije između nadmorske visine i starosti češera značajne (p = 0,05). Kao rezultat značajne interakcije između dviju skupina, srednji test razdvajanja (Tukey test) pokazao je da je najveća klijavost bila 89,0% u kontrolnoj skupini, a najniža 77,5% u 7-godišnjim češerima. U zoni viših nadmorskih visina najveća klijavost iznosila je 74,0%, a najmanja 71,0%. Dobivena je iz sjemena starih 5 odnosno 7 godina. Općenito, vrijednosti klijavosti u zoni niže nadmorske visine bile su veće od onih u zoni više nadmorske visine. Međutim, smanjenje vrijednosti klijavosti od kontrolnog do 7-godišnjeg sjemena bilo je veće u zoni niže nadmorske visine nego u zoni više nadmorske visine. Studija je pokazala da je na stopu klijanja i postotak, nadmorska visina učinkovitija od starosti češera.

2-metoksi-1,4-naftokinon (2-MNQ) je prirodni derivat 1,4-naftokinona koji je u dosad provedenim ispitivanjima pokazao antipruritično, protuupalno, antialergijsko, antimikrobno i antikancerogeno djelovanje. U ovom istraživanju ispitan je utjecaj 2-MNQ na klijanje sjemenki i razinu oksidacijskog stresa u klijanaca. Kao biljni model korištene su sjemenke dviju biljnih vrsta: bijele gorušice (Sinapis alba L.) i rotkvice (Raphanus sativus L.). Sjemenke su bile izložene 2-MNQ u koncentracijama 1, 5, 10, 20, 30 i 40 μg/ml kroz 3 dana. U pokus su bile uključene negativna kontrola (sjemenke izložene 3 dana destiliranoj vodi) i pozitivna kontrola (sjemenke izložene 3 dana 0,02M otopini CuSO₄). Nakon 3-dnevne izloženosti, za obje biljne vrste određena je klijavost (%) i duljina korjenčića (mm) te je u homogenatu klijanaca određena katalitička aktivnost SOD (U/ml). Podaci su statistički obrađeni pomoću jednosmjerne analize varijance (ANOVA test) te primjenom Duncan's New Multiple Range Test-a. Također, određena je korelacija između mjerenih parametara te analiza glavnih komponenti. Statistički značajnim smatrane su vrijednosti koje se razlikuju na razini p 0,05. Povećanjem koncentracije 2-MNQ opao je broj proklijalih sjemenki bijele gorušice i sukladno tome padala je duljina korjenčića, a 2-MNQ u koncentraciji 30 i 40 μg/ml u potpunosti je inhibirao klijavost sjemenki. To ukazuje da 2-MNQ inhibira klijanje sjemenki bijele gorušice. S druge strane 2-MNQ nije imao utjecaja na klijanje rotkvice. U klijanaca bijele gorušice s koncentracijom 2-MNQ rasla je aktivnost SOD-a te je najveća aktivnost SOD-a izmjerena pri koncentracijama 2-MNQ od 1 i 10 μg/ml. Iz navedenog se može zaključiti kako je biljka tada bila izložena najvećem oksidacijskom stresu te je bilo potrebno proizvesti veliku količinu SOD-a kako bi se od njega obranila i samim time omogućila sjemenkama da prokliju. Katalitička aktivnost SOD-a u rotkvici pri svim koncentracijama otopine 2-MNQ približno je bila ista te nisu uočena odstupanja. Utjecaj 2-MNQ na klijavost sjemenki je različit te ovisi i o koncentraciji 2-MNQ i o samoj biljnoj vrsti. Bijela gorušica osjetljivija je na djelovanje 2-MNQ, dok 2-MNQ u ispitivanim koncentracijama nije imao utjecaja na klijavost rotkvice. Mehanizam toksičnog učinka 2-MNQ može se povezati s oksidacijskim stresom s obzirom da je tretman s 2-MNQ doveo do promjene aktivnosti SOD-a u bijeloj gorušici. 2-metoxy-1,4-naphtoquinone (2-MNQ) is a natural derivate of 1,4-naphthoquinone which has shown antipruritic, anti-inflammatory, anti-allergic, antimicrobial and anti-cancer effects.In this study, the influence of 2-MNQ on seed germination and the level of oxidative stress was investigated. As a plant model the seeds of two plant species were used: white mustard (Sinapis alba L.) and radish (Raphanus sativus L.). The seeds were exposed to 2-MNQ in concentrations of 1, 5, 10, 20, 30 and 40 μg/ml for 3 days. The study included a negative control (seeds exposed to distilled water for 3 days) and a positive control (seeds exposed to 0.02M CuSO₄ solution for 3 days). After a 3-day exposure, germination (%) and root length (mm) were measured, and in seedlings homogenate catalytic activity of SOD (U/ml) was determined. Data were statistically processed using one-way analysis of variance (ANOVA test) and Duncan's New Multiple Range Test as well as tested for correlation and Principal Component Analysis. Significance level was set at p ≤ 0.05. With the increase of 2-MNQ concentration the number of germinated seeds of white mustard decreased and 2-MNQ in concentrations of 30 and 40 μg/ml completely ceased germination of white mustard. In parallel the decrease in the length of the radicle with the increase of 2-MNQ concentration was recorded. Thus, 2-MNQ inhibits the germination of white mustard seeds. On contrary, 2-MNQ had no impact on germination of radish. In white mustard seedlings with the increase of 2-MNQ concentration the increase of SOD activity is observed, and the highest SOD activity is recorded at 2-MNQ concentrations 1 and 10 μg/ml. Therefore, it can be concluded that the plant was then exposed to the greatest oxidative stress, and it was necessary to produce a large amount of SOD in order to defend against it and at the same time enable germination. In radish seedlings the catalytic activity of SOD at all concentrations of the 2-MNQ was approximately the same, with no deviations. The impact of 2-MNQ on the seed germination is different and depends on the concentration of 2-MNQ and the plant species itself. White mustard is more sensitive to 2-MNQ, while 2-MNQ in concentrations tested had no impact on radish. The mechanism of the toxic effect of 2-MNQ can be related to oxidative stress, considering that treatment with 2-MNQ led to a change in SOD activity in white mustard.

The present study focused on detecting bacteria of the Xanthomonas spp. complex (X. euvesicatoria, X. vesicatoria, X. perforans and X. gardneri) and examining their influence on certain quality parameters of pepper seed collected from the territory of Smederevska Palanka (Serbia). The analysis included 27 non-commercial pepper seed genotypes (including chili and sweet pepper) collected in 2021. Several parameters of the quality of analyzed pepper seed (germination energy, total germination, moisture and seed health) were determined. The results showed that out of a total of 27 analyzed samples of pepper seed, the presence of X. euvesicatoria was detected in 13 of them. The presence of X. vesicatoria, X. gardneri and X. perforans was not confirmed. Germination energy of infected seed was 52-84%, and of bacteria-free seed 63-90%; total germination of infected seed was 66-91%, and of bacteria-free seed 80-95%. Seed moisture of infected seed samples was 6.1-12%, and of bacteria-free seed 6.2-8.1%. These parameters did not show significant statistical difference (p>0.05). The presence of seed-borne fungi Fusarium sp. accounted for up to 3% in 25 samples, while it was up to 6% in the remaining two; Alternaria sp. ranked from 1-4% in 25 samples, and up to 5% in only two samples. The results led to a conclusion that the bacterium X. euvesicatoria is the predominant pathogen of the Xanthomonas spp. complex, but it did not affect the quality parameters of the tested pepper seed. U ovom radu izvršena je detekcija bakterija iz kompleksa Xanthomonas (X. euvesicatoria, X. vesicatoria, X. perforans i X. gardneri) i ispitan uticaj prisustva bakterija na određene parametre kvaliteta semena paprike poreklom sa teritorije Smederevske Palanke (Srbija). Analiza je obuhvatila 27 nekomercijalnih genotipova semena paprike (uključujući čili i slatku papriku) prikupljenih u sezoni 2021. godine. U radu su ocenjeni parametri kvaliteta semena paprike i to energija klijanja, ukupna klijavost, vlaga i zdravstvena ispravnost. Rezultati su pokazali da je od ukupno 27 analiziranih uzoraka semena paprike, prisustvo X. euvesicatoria detektovano kod ukupno 13 uzoraka. Prisustvo X. vesicatoria, X. gardneri i X. perforans nije utvrđeno ni u jednom uzorku semena paprike. Energija klijanja zaraženog semena je iznosila od 52-84%, a kod semena bez prisustva bakterija od 63-90%; ukupna klijavost u zaraženom semenu je bila od 66-91%, a u semenu bez prisustva bakterija 80-95%. Vlažnost semena u zaraženim uzorcima je iznosila 6,1-12%, a u uzorcima bez prisustva bakterija između 6,2-8,1%. Utvrđivani parametri kvaliteta se nisu značajno razlikovali na statističkom nivou (p>0,05). Prisustvo fitopatogenih gljiva koje se prenose semenom je utvrđeno u svim uzorcima semena paprike, i to Fusarium sp. do 3% kod ukupno 25 uzoraka, dok je kod dva uzorka zaraza bila i do 6%; prisustvo Alternaria sp. je bilo od 1-4% kod ukupno 25, a kod dva uzorka više, do 5%. Dobijeni rezultati ukazuju da je bakterija X. euvesicatoria dominantan patogen iz kompleksa Xanthomonas vrsta, ali da utvrđeno prisustvo nije značajno uticalo na parametre kvaliteta semena paprike

Cilj istraživanja bio je utvrditi alelopatski potencijal lucerne (Medicago sativa L.) na klijavost sjemena i rast klijanaca rotkvice (Raphanus sativus L.), radiča (Cichorium intybus L. var. foliosum) i rajčice (Lycopersicon esculentum Mill.). Vodeni ekstrakti pripremljeni od suhe nadzemne mase lucerne u tri koncentracije (1 %, 2,5 % i 5 %) testirani su u laboratorijskim uvjetima. Alelopatski potencijal lucerne ovisio je o test vrsti, koncentraciji vodenog ekstrakta te mjerenom parametru. Najslabije djelovanje zabilježeno je na klijavost te rast klijanaca rajčice. Povećanjem koncentracije vodenog ekstrakta povećavao se i negativni alelopatski potencijal. Najmanji utjecaj vodenih ekstrakata zabilježen je na suhu masu klijanaca test vrsta., The aim of the study was to determine the allelopathic potential of alfalfa (Medicago sativa L.) on seed germination and seedling growth of radish (Raphanus sativus L.), radicchio (Cichorium intybus L. var. foliosum) and tomato (Lycopersicon esculentum Mill.). Water extracts prepared from dry alfalfa biomass in three concentrations (1%, 2.5% and 5%) were tested under laboratory conditions. The allelopathic potential of alfalfa depended on the test species, the water extract concentration, and the measured parameter. The weakest effect was recorded on germination and growth of tomato seedlings. As the concentration of the water extract increased, the negative allelopathic potential also increased. Dry weight of seedlings of test species was the least affected.

Cilj rada bio je odrediti utjecaj hidrolata na inhibiciju klijanja sjemena invazivne korovne vrste, običnog dvozuba (Bidens tripartita L.). Istraživanje je provedeno u kontroliranim laboratorijskim uvjetima. U svrhu istraživanja korišteni su hidrolati lavande (Lavandula angustifolia) i smilja (Helichrysum italicum) pomiješani s destiliranom vodom u različitim omjerima (1:1), (1:4) i (1:8). Klijavost je provjerena nakon 4. i 6. dana od postavljanja pokusa. Najbolji inhibicijski utjecaj na klijanje sjemena običnog dvozuba imala je otopina hidrolata lavande u omjeru 1:1. Nakon 4. dana postotak klijavosti iznosio je 1.20%, a nakon 6. dana 8.20%. Dobiveni rezultati u laboratorijskim uvjetima sugeriraju mogućnost korištenja hidrolata lavande kao bioherbicida. Međutim, za potvrdu ovih rezultata potrebno je obaviti i terenska istraživanja. The aim of the work was to determine the influence of hydrolates on the inhibition off seed germination of the invasive weed species Three-lobed Beggarticks (Bidens tripartita L.). The research was performed in controlled laboratory conditions. Hydrolates of lavander (Lavandula angustifolia) and immortelle (Helichrysum italicum) mixed with distilled water in different proportions were used for the purpose of research work. Germination of seeds took place under sterile, laboratory conditions. Seeds were placed in Petri dishes on filter paper. For the purpose of research, 5 control Petri dishes (seed soaked in distilled water) were placed, 5 Petri dishes each for hydrolates of lavander and immortelle with their dilutions in the ratios (1:1), (1:4) and (1:8). The best inhibitory effect on the seed germination of Three-lobed Beggarticks was the lavander hydrolate solution in a ratio of 1:1. After the 4th day, the percentage of germination was 1.20%, and after the 6th day, 8.20%. The results obtained in laboratory conditions suggest the possibility of using lavander hydrolate as a bioherbicide. However, to confirm these results, it is necessary to carry out field research.

Muškatna kadulja je dvogodišnja ljekovita i aromatična biljka iz porodice Lamiaceae. Eterično ulje se koristi u prehrambenoj i farmaceutskoj industriji, aromaterapiji i tradicionalnoj medicini. Ima antioksidativno, antimikrobno i citotoksično djelovanje. U Kolekciji ljekovitog i aromatičnog bilja Zavoda za sjemenarstvo Sveučilišta u Zagrebu Agronomskog fakulteta čuva se sjeme primki muškatne kadulje s različitih lokacija Republike Hrvatske, kao i onih iz drugih dijelova Europe, a neke su stare preko 20 godina. S obzirom na starost sjemena, u ovom je radu ispitivana klijavost i energija klijanja sjemena. U istraživanju je korišteno sjeme 32 primke muškatne kadulje iz Kolekcije ljekovitog i aromatičnog bilja. Na kraju provedenog istraživanja izračunata je klijavost (G, %) i prosječno vrijeme klijanja (MGT, dan). Najveći postotak klijavosti utvrđen je kod primki 2386 (94 %) s Dugog Otoka i 2393 (90 %) iz Tribunja koje se čuvaju od 2009. godine, zatim primki 2202 (76 %) s Korčule, 2205 (70 %) s Brača, 2201 (68 %) s Korčule, 2199 (68 %) sa Šolte, 2219 (62 %) s Dugog otoka i 2198 (62 %) sa Šolte koje se čuvaju od 2008. godine. Ostale primke imale su klijavost oko 50 % ili manje od toga. Na temelju provedenog istraživanja utvrđeno je da sjeme muškatne kadulje ima vrlo dobru klijavost što znači da dobro podnosi čuvanje, odnosno da je čuvano u dobrim uvjetima te se regeneracije u bankama biljnih gena ne trebaju često provoditi. Clary sage (Salvia sclarea L.) is a biannual aromatic plant of the Lamiaceae family. The essential oil is used in the food and pharmaceutical industries, aromatherapy and traditional medicine. It has antioxidant, antimicrobial and cytotoxic effects. In the Collection of Medicinal and Aromatic Plants of the Department of Seed Science, University of Zagreb Faculty of Agriculture, seeds of clary sage from various locations in the Republic of Croatia and Europe are kept, some of which are over 20 years old. Given the age of the seeds, the germination capacity and germination energy were determined. In the research seed of 32 accessions of clary sage from the Collection of Medicinal and Aromatic Plants was used. At the end of the research, germination (G, %) and mean germination time (MGT, day) were calculated. The highest percentage of germination was observed in accessions 2386 (94 %) from Dugi otok and 2393 (90 %) from Tribunj, which have been conserved since 2009, then 2202 (76 %) from Korčula, 2205 (70 %) from Brač, 2201 (68 %) from Korčula, 2199 (62 %) from Šolta, 2219 (62 %) from Dugi otok and 2198 (62 %) from Šolta conserved since 2008. Other accessions had germination rates of about 50 % or less. Based on the research carried out, it was conducted that clary sage seeds have very good germination, which means that they tolerate storage very well, that they have been stored in good conditions and that regeneration in plant gene banks should not be carried out often.

Neven (Calendula officinalis L.) je poznat po svojim ljekovitim svojstvima kao i primjeni u medicinskoj, kemijskoj, kozmetičkoj i prehrambenoj industriji. Prilagođen je umjerenoj klimi te mu je optimalna temperatura klijanja 20 °C. Klijanje sjemena i rani rast sadnica su najosjetljiviji stadiji na abiotske stresove, kao što su nepovoljna temperatura i suša, a predsjetveni tretmani sjemena pozitivno utječu na svojstva klijavosti ili kvalitetu sjemena i sadnica u stresnim uvjetima. S obzirom da se sjetva nevena obavlja čim se uspostave minimalni uvjeti, a tada temperature i količine oborina mogu biti znatno niže od optimalnih, cilj ovog istraživanja bio je utvrditi utjecaj predtretmana na svojstva klijavosti sjemena nevena u uvjetima niskih temperatura i suše (-0,8 MPa PEG 6000). Laboratorijskim pokusom istražen je utjecaj temperature (14 °C, 18 °C i 22 °C) na klijavost sjemena nevena uz predtretmane dH2O i GA3 (250 ppm) u trajanju od 24 h. Netretirano sjeme je predstavljalo kontrolu. Utvrđeno je da sjeme nevena ne klije u uvjetima suše bez obzira na predsjetveni tretman i na temperaturu pri kojoj je klijalo. Ni temperatura niti predtretman sjemena ne utječu značajno na broj proklijalih sjemenki nevena, ali utječu na brzinu klijanja.

In this study the confectionery sunflower seed was tested to germination at different pH media, from 3.5 to 8.5. The experiment was set up at room temperature (22°C) and at 24 h dark conditions for 10 days. The mean germination rate was 76%. The highest germination rate was 86% at pH 3.5, whereas the lowest was 68% at pH 5.5. The ANOVA resulted with very significant influence of pH on sunflower seedlings root, stem and total length (p, Cilj ovog istraživanja bio je utvrditi klijavost proteinskog tipa suncokreta na različitim pH vrijednostima (3,5 - 8,5). Eksperiment je postavljen na sobnoj temperaturi (22°C) i to u mraku 24 h kroz 10 dana. Ukupna klijavost proteinskog suncokreta bila je 76%. Najviša klijavost iznosila je 86% pri pH 3,5, dok je najniža bila 68% pri pH 5,5. ANOVA je rezultirala vrlo značajnim utjecajem pH na korijen, stabljiku i ukupnu duljinu klijanaca proteinskog tipa suncokreta (p

The aim of this paper was to examine the influence of the electrostatic field on seeds of soybean (Glycine max L), vetch (Vicia spp. L.), pea (Pisum sativum L.), grass pea (Lathyrus sativus L.) and chickpea (Cicer arietinum L.) which were selected at the Institute of Field and Vegetable Crops in Novi Sad. The DC voltage of 9V was used. The durations of seed treatment were 0 (control), 1, 2 and 3 minutes. The trial was set up as a randomized block design with four replications. 4x100 seeds of each variant and control (untreated seed) were treated. After treatment, seed quality was examined using a germination test (optimal temperature) and a cold test (low temperature). The results of the study showed that the effect of the electrostatic field on seed quality depended on the plant species, the time of seed treatment and the temperature conditions in which the seed germinated after treatment. The increase in germination energy ranged up to 18.18% in vetch, and a decrease of up to 12% was observed in chickpea. The application of the electrostatic field had a significantly greater impact on seeds that were exposed to low temperatures in the germination process after treatment. The increase in seed germination ranged up to 82.35% in chickpeas, and the decrease amounted to 92.68% in peas. In addition, the obtained results indicate that it is not possible to talk about the universal application of a certain duration of seed treatment. Cilj rada je bio ispitivanje uticaja elektrostatičkog polja na seme soje (Glycine max L.), grahorice (Vicia spp. L.), graška (Pisum sativum L.), sastrice (Lathyrus sativus L.) i nauta (Cicer arietinum L.) koji su selekcionisani na Institutu za ratarstvo i povrtarstvo u Novom Sadu. Za tretiranje je korišćen jednosmerni napon jačine 9 V. Vreme tretiranja semena iznosilo je 0 (kontrola), 1, 2 i 3 minuta. Nakon tretiranja, ispitan je kvalitet semena primenom standardnog testa klijavosti (optimalna temperatura) i hladnog testa (niska temperatura). Rezultati istraživanja su pokazali da je efekat elektrostatičkog polja na kvalitet semena zavisio od biljne vrste, vremena tretiranja semena i temperaturnih uslova u kojima je seme klijalo nakon tretiranja. Povećanje energije klijanja se kretalo do 18,18% kod grahorice, a smanjenje do 12% kod nauta. Značajno veći uticaj primena elektrostatičkog polja imala je na semenu koje je posle tretiranja izloženo niskoj temperaturi u procesu klijanja. Povećanje klijavosti semena se kretalo do 82,35% kod nauta, a smanjenje do 92,68% kod graška. Takođe, dobijeni rezultati ukazuju na to da se ne može govoriti o univerzalnoj primeni određenog vremena tretiranja, jer se može dogoditi da ono ne odgovara određenoj biljnoj vrsti, pa može doći do pogoršanja kvaliteta semena.

Information regarding the adaptation of maize (Zea mays L.) inbred lines to various field conditions and seed longevity is essential for seed companies. The objective of this work was to evaluate different vigour tests for detecting differences among naturally aged seeds and to assess the relationship between seed quality parameters and pedigree data of maize inbred lines. A seed of 15 maize inbred lines, selected at Maize Research Institute Zemun Polje, belonging to different heterotic groups was produced during 2011. Seed quality was assessed in 2015 by standard ger mination, radical emergence, accelerated ageing, and cold test. Root length of 4-day old seedlings was measured in all applied tests. Analysis of variance showed that genotype and storage condi tions had a significant effect on all seed quality parameters. Large variation among inbred lines was detected in seed vigour determined by three tests, in particular by the accelerated ageing test. Cold test results showed very good chilling tolerance of tested genotypes, except two inbred lines with Lancaster background. The correlation between the cold test and the accelerated ageing test was significant, while the radical emergence test had a poor correlation with them. Obtained results indicate that the application of either cold test or accelerated ageing test is reliable for screening seed vigour of maize inbred lines. Semenskim kompanijama su od izuzetne važnosti informacije o adaptibilnosti samooplod- nih linija kukuruza (Zea mays L ) različitim uslovima u polju i dugovečnosti semena Cilj ovog rada bio je da se izvrši procena vigor testova za otkrivanje razlika između prirodno ostarelog semena u različitim uslovima čuvanja i da se proceni odnos između parametara kvaliteta semena i podataka o pedigreu samooplodnih linija kukuruza Seme 15 samooplodnih linija kukuruza selekcionisanih u “Institutu za kukuruz” - Zemun Polje, koje pripadaju različitim heterotičnim grupama, proizvedeno je 2011 godine Kvalitet semena je ocenjivan 2015 godine primenom standardnog testa za klijavost, testa za pojavu primarnog korena, ubrzanim starenjem i hlad- nim testom U svim primenjenim ispitivanjima merena je dužina korena klijanaca starih 4 dana Analiza varijanse je pokazala da genotip i uslovi čuvanja semena imaju značajan uticaj na sve parametre kvaliteta semena Samooplodne linije kukuruza su se značajno razlikovale u pogledu vigora semena na osnovu tri primenjena testa, a naročito testa ubrzanog starenja Rezultati hlad- nog testa su pokazali visoku tolerantnost ispitivanih genotipova na niske temperature prilikom nicanja, osim dve samooplodne linije Lancaster porekla Korelacija između hladnog testa i testa ubrzanog starenja bila je značajna, dok je test pojave primarnog korena imao slabu korelaciju sa njima Dobijeni rezultati ukazuju da je primena hladnog testa i testa ubrzanog starenja pouzdana za skrining vigora semena samooplodnih linija kukuruza