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This diploma thesis entitled "The impact of acne on everyday life of students in a selected high school" is divided into two main parts. The first theoretical part is focused on the characteristics of the disease and on the impact of this disease on the quality of life of patients. There is a brief characterization of skin structure, it provides information about pathogenesis of acne and triggering factors, its clinical manifestations and clinical picture. In this part we can also find the diagnosis and treatment options for acne and an overview of other selected facial dermatoses. This part includes the effect of acne on patients' quality of life and an overview of the most common myths about acne. The second part is practically oriented. I used an online questionnaire to find out the impact of acne on everyday life of students in a selected high school. The results of my questionnaire survey show that 12 students (37,5 %) out of 32 respondents suffering from acne (100 %) reached the limit set by me, which determined whether their lives are affected by this disease or not. The first partial goal of the work was to determine the students' knowledge of this disease. I found out that the success rate (evaluated by the number of correct answers) of students in the knowledge part of the questionnaire...

First and last name: Karel Fošumpaur Supervisor: Mgr. Jakub Jeníček, Ph.D. Opponent of the thesis: Name of the thesis: Effect of electrical stimulation on soft tissue healing Abstract of the thesis: This bachelor thesis summarizes the current state of knowledge regarding the effectiveness of electrical stimulation on soft tissue healing by providing an overview of the issue. The aim of the thesis is to describe the current level of research on the subject in terms of its completeness, current stage and limitations. Fifteen peer-reviewed, relevant articles were included and analyzed through a systematic search. Through analytical analysis and evaluation of the studies, it is determined whether and under what conditions electrical stimulation is effective for soft tissue healing. The articles presented include studies testing the effectiveness of electrical stimulation for healing structurally damaged muscle, tendon, skin and ligament. The work provides a largely positive conclusion regarding the healing effect of electrical stimulation. Key words: electrical stimulation, healing, soft tissue, muscle, tendon, skin, connective tissue, ligament, regeneration, reparation, electrotherapy

Author: Daniela Stoklasová Institution: Department of non-medical studies Faculty od Medicine in Hradec Králové Title: Quality of life in vitiligo patients Supervisor: prof. PhDr. Jiří Mareš, CSc. Number of pages: 106 Year of defense: 2022 Keywords: vitiligo, quality of life, skin, multifactorial disease, nursing care The bachelor's thesis deals with the quality of life of patients with vitiligo. The theoretical part deals with the anatomy and function of the skin. Furthermore, the problems of vitiligo, the causes of this disease, the clinical picture, diagnosis, treatment, similar diseases and autoimmune diseases associated with vitiligo are explained. It also deals with nursing care for these patients and their self-care. Lastly, it clarifies the concept of quality of life, its history, how we generally evaluate quality of life and how we can evaluate it in patients with vitiligo, and also what effect this disease has on the patient's quality of life. The empirical part describes the results of quantitative research, which took place in 52 patients using the Czech version of the VitiQoL questionnaire, which was translated into Czech with the consent of foreign authors. Besides the overall quality of life, this bachelor's thesis focuses on specific areas of quality of life that are highly relevant...

The topic of my bachelor's thesis is "Issues of atopic dermatitis in context of nutrition." Atopic dermatitis is a type of skin inflammation in moist areas (back of the knee, inner elbow, groin). Skin health is affected by factors including the weather, textile and diet. Data were gathered using a qualitative research and snowball sampling. A survey was conducted among nine respondents, each providing information on the food they ate for a week. The goal was to map atopic dermatitis in the context of nutrition. The practical part of the thesis focused on what foods seem to be the most problematic for respondents. The survey showed these include citrus fruit, sweets, highly processed foods and especially alcohol. Respondents said that wine was the most problematic among alcoholic beverages. A few respondents mentioned nuts, soya and dairy. Seasons also have an impact on the atopic dermatitis incidence, with winter posing the biggest problem and summer following. A link between atopic dermatitis and the weather has not been properly studied and cannot be confirmed. The food analysis was recorded in the Nutriservis app. Data were entered into an Excel table that includes energy and macronutrient values of individual meals. The analysis shows that most respondents eat bread/rolls with ham or cheese, or cereal for breakfast. Snacks are similar but smaller than breakfasts. Lunches and dinners are often home-made meals consisting of good polysaccharides (rice, pasta, potatoes), meat (mostly chicken) and vegetables. The analysis reveals often unbalanced energy intake over the week, mostly because of alcohol consumption. Most respondents eat enough fruit and vegetables.

Kallikrein-related peptidases (KLKs) are a subgroup of serine proteases of undisputable importance for a variety of functions, whose dysregulation has been linked to several pathological phenotypes. Among those pathologies, the Netherton syndrome stands out, since it is one of the very few that has its mechanism directly linked to KLK proteases as the main culprit of the disease, namely KLK5, KLK7 and to a lesser degree, KLK14. In this case, a mutation in the SPINK5 gene leads to uncontrolled hyperactivity of those proteases, which results in epidermal barrier breach due to excessive epidermal desquamation and severe inflammation of the skin. Inflammation mechanisms of NS are still relatively poorly understood, with important roles being attributed to the activities of KLKs in the processing of immune system molecules and also to the dysregulation of the cutaneous microbiome. TNFα signalling plays a key role in the homeostasis and immune response in the skin. Chronic skin infections may lead to deleterious effects with strong participation of TNFα signalling. To address the degree of its effects on the pathogenesis of NS, we have created a mouse model where the TNFR1 is disrupted by knockout of the Tnfr1 gene on the background of a previously established mouse model of the Netherton syndrome. We...

Helminth neuroinfections represent a serious health issue, but the mechanisms of the host immune response often remain neglected despite the fact they might contribute to pathogenesis. This is partly due to the unavailability of clinical samples and the lack of suitable laboratory models. Herein, I focused on the characterization of several aspects of the immune response of mice infected with the neuropathogenic avian schistosome Trichobilharzia regenti. After the percutaneous infection of mice (accidental hosts), most T. regenti schistosomula are entrapped and eliminated in the skin, but the parasite antigens initiating the protective immune reaction are not known. Our in vitro experiments revealed that T. regenti cathepsin B2, a cysteine peptidase used for the skin penetration, activates bone marrow-derived dendritic cells much stronger than the parasite homogenate, suggesting its role in initiating the mixed type1/2 host immune response. However, some schistosomula manage to escape from the skin and continue their migration to the spinal cord. Here they crawl preferentially within the white matter which we demonstrated by the robust 3D imaging techniques, ultramicroscopy and micro-CT. The invasion of the spinal cord is accompanied by striking hypertrophy of astrocytes and microglia. We showed...

The surface of the human body is colonized by a large number of microorganisms whose composition depends not only on external and internal factors, but is also significantly influenced by the topography of human skin. The complex skin microbiota is an essential part of physiological and protective mechanisms of the skin. The change in the dynamics of microbial communities on the skin or in the gastrointestinal tract is currently considered to be part of triggering mechanisms of many skin diseases. Some of the skin inflammatory diseases are directly associated with a shift of skin microbiota composition - for instance atopic dermatitis, acne vulgaris or psoriasis. Gaining and perceiving knowledge about interspecies interactions and their effect on a host could lead to the development of new diagnostic and therapeutic approaches which could make the prevention or treatment of some skin disorders more effective. Key words: skin, skin diseases, skin microbiota, immunity, psoriasis, atopic dermatitis, vitiligo

Skin is a natural and effective barrier of the body against the adverse effects of the external environment. Which, however, requires its constant regeneration and, in the event of damage, repair. Stem cells stored in the epidermis, hair follicles and dermis contribute to this. As with other stem cells, these are characterized by so-called stemness, ie the ability to self-renew and differentiate into other cell types, thus providing a source of cells for skin renewal. During aging, due to internal and external factors (mainly due to oxidative stress and DNA damage), the integrity and functionality of the skin barrier are lost. This process is related, among other things, to a reduction in the number and function of skin stem cells. Today several therapeutic approaches are being developed that use stem cells, but at the same time, it is clear that their origin also significantly affects their use. Therefore is necessary to have a good understanding of the specific properties of the function of skin stem cells to modulate the properties of the skin. This work aims to create a review of scientific literature, which covers the topic of skin stem cells, their role in the processes of regeneration and repair and their role in aging. The work will also address the issue of the skin or other types of stem...

The Bachelor thesis will deal with subculture of tattoo artist, its lifestyle, political opinions and methods of tattoo and their meaning. theoretical part will include a clarification of the basic terms related to the issue. Further is the thesis focused on brief description of tattoo´s technics its source of finding with examples. In practical part of bachelor htesis is made qualitative research via interview with tattoo artist. The aim of my work will be to map and clarify the value and representative system the subcultures around Tábor The aim will be achieved thanks to interpretation of interviews with tattoo artists. I will make these interviews with chosen respondents who live around Tábor and who tattoo professionally or it is just their hobby. In these interviews I will be especially interested in style of tattooing and what is motivation for tattooing. Other areas will be political and value orientations that are generally closely related to subcultures.

Wound healing of skin in mammals and its regeneration in Amphibians are crucial biomedicine topics in the last few decades. The most important aspect in humans is the scrarring proccess and the effort to substitute it with the regeneration producing functional and differentiated tissues. To modulate the formation of scar it is neccessary to compare both proccesses. The core animal model is axolotl (Caudata) where regeneration takes place during whole lifespam. On the other hand, in frogs (Anurans) this phenomenon is restricted up to metamorphosis. After metamorphosis, the immune system of Anurans is similar if compared to mammals. Similarly, the transition from early embryonic development in mammals, where the fetus was able to completely regenerate damaged tissue to an adult type of healing, goes hand-in-hand with the development of the immune system and structural differentiation of damaged tissue. Thus, the inflammatory cells and their regulation, the formation of ECM, which includes fibroblast proliferation, and the production of appropriate cytokines are key factors that distinguish the process of healing with and without scarring.

The doctoral thesis MD. Liubov Kastnerova (previous name Kyrpychova) is focused on the histomorphological and molecular biologic features of selected cutaneous epithelial and nonepithelial tumors and is structured as a commentary to the 20 articles published during four years, representing the completed scientific projects in the Ph.D. course. In eight papers, the author of the thesis is the first author, whereas she coauthored in the remaining 12 papers. The thesis is composed of the commented files of authors own publications and it is divided into cutaneous epithelial and nonepithelial tumors. The first section, «Cutaneous epithelial tumors», includes 14 articles that are subdivided into two parts: adnexal tumors (9 articles) and lesions of anogenital mammary-like glands (5 articles). Of the nine articles on adnexal tumors, there are 5 articles focused on various benign and malignant adnexal lesions with apocrine or eccrine differentiation. Novel findings in this part include the identification of hitherto unreported alterations of the MYBL1 gene in adenoid cystic carcinoma of the skin and lack of deletion of the 1p36 locus in this neoplasm; the lack of a correlation between cellular composition and the presence CRTC1-MAML2 fusions in hidradenoma, the absence of CRTC3-MAML2 fusions in this tumor,...

This bachelor thesis deals with one of the most frequent complications of patients undergoing radiotherapy. Despite all the progress and development of this treatment, unfortunately, the radiation influences not only the tumour itself but has an effect on surrounding healthy tissue as well. Post-radiational reaction on skin arises. The aim of this thesis is to discover particularities of nursing care of patients with post-radiational changes on skin, to discover practical experience of medical personnel with products for prevention and treatment of post-radiational changes on skin and discover the role of nurses in the issues of skin treatment of patients undergoing the radiotherapy. The thesis is divided in theoretical part and empirical part. The theoretical part deals with types of tumours, diagnostics and treatment, undesirable effects of ionizing radiation and nursing care of patients undergoing the radiotherapy. A qualitative research inquiry was accomplished by means of semi structured interviews in the empirical part. The research sample was intentionally chosen and the criterion for the choice was a nurse working at an oncological inpatient department or at a radiotherapeutic outpatient department. The research group was created by 6 nurses from different medical facilities in the Czech Republic. Four categories and seven subcategories were created while assessing the interviews. The results of the research inquiry show that the most recommended product for preventive skin treatment is a Cavilon spray. It is important to use water-based products instead of oil-based ones. Under the principles of skin treatment, it is most frequently recommended to wear cotton and loose-fitting clothes without laces and shave only with electric razor not with razor blades. Furthermore, the careful hygiene with cold water without using a soap or scented soap is also important. It is important for every medical staff member to approach individually to every patient's needs. One of the main roles of a nurse is the right patient instruction, right evaluation of skin damage and skin reaction treatment. We recommend to lay stress on the prevention in the form of instruction of the patient already before the start of the radioactive treatment.

Diploma thesis is devoted to the analysis of triacylglycerol estolides (TG-est) by HPLC/MS. These lipids contain four ester bonds. TG-est are part of some vegetable oils and in mammals function as a storage form of metabolically important fatty acid estolides. The aim of this thesis was to prove the existence of TG-est in vernix caseosa and explore the possibilities of analysis in this material. Standards of TG-est were studied with using high-resolution and tandem mass spectrometry. Was suggested and optimized HPLC method for TG-est in a reverse nonaqueous system with the use of column Kinetex C18 and gradient elution with acetonitrile and 2-propanol. This method was used for analysis vernix caseosa fraction, and it was shown that TG-est are present in the sample. Attempts to simplify the lipid sample before analysis HPLC/MS by TLC fractionation were not very successful.

The aim of this diploma thesis was to summarize the basic knowledge of human skin and it's biophysical parameters. Attention was focused on influence of cosmetic products with different pH on the change of skin parameters especially. The first part describes the anatomical and histological structure of the skin, it's basic functions and development. In the following chapters of this work is mentioned physiological representation of microorganisms found on the skin surface and general characteristics of cosmetic products. There are used in everyday skin care that affect the natural skin surface pH value. Optimum surface pH is important for the proper functioning of human skin. The final chapters of the described the characteristics of the basic biophysical parameters of the skin (hydration, TEWL, pH, sebum) and methods for their measurement. Key words: skin, transepidermal water loss, pH, hydration, sebum, microbiome

Methods of analytical chemistry are widely used in lipidomics. Separation techniques coupled to mass spectrometry or nuclear magnetic resonance are used very often. They make it possible to identify lipids present in the matrix in very small quantities. This work summarizes the application of modern analytical methods and instrumentation for identifying and characterizing lipids in vernix caseosa. It is shown how I contributed during the Ph.D. studies to the elucidation of the structure and characterization of unknown lipid classes followed by more detailed description of those lipid classes already identified in vernix caseosa. An integral part of my work was the application of the method enabling the localization of double bonds developed by our laboratory in triacylglycerols and 1,2-diol diesters in vernix caseosa. This analytical method is based on the formation of an acetonitrile adduct in an ionization source of a mass spectrometer enabling atmospheric pressure ionization. The complexity of the triacylglycerol class did not allow a complete characterization of the double bonds. However, the fragmentation mostly showed that double bonds up to n-12 position are present, but small peaks in some spectra also indicated double bonds at more distant positions from the chain termini. I have also...

The character of oncological diseases can be benign or malignant. Both variants have fundamental consequences for the patient. If the changes in the skin are malignant, treatment is necessary. In case the medical finding is benign, it depends on the patient whether the tumour would have it removed or not. In any case, the patient must be informed about all risks and especially the dispensarisation which is the most significant factor for early diagnosis and effective treatment. Oncological disease of skin entails many significant changes in the patient's life. The patient's order of values, lifestyle, as well as perception of life changes. In this difficult situation, the support of the patients from the side of family and loved ones, as well as of the medical personnel, is crucial. The patient's need of security and safety must be satisfied, as it is a significant factor in coping with the disease development from diagnostics to treatment and eventual dying. The concept of the bachelor thesis is theoretical and based on the study and analysis of information sources related to the issues of oncological disease of skin. To introduce the largest and very important organ the skin its anatomy and physiology is briefly described at the beginning. Subsequently, oncological diseases of skin, divided into benign tumours, their variants and characteristics, are outlined. The subchapter of malign tumours lists the most frequent malignant skin tumours, i.e. basal cell carcinoma (BCC), squamous cell carcinoma (SCC), and melanoma. Again, their characteristic and seriousness is described. Diagnostics and therapy of oncological diseases of skin is worth mentioning. The prognosis of the entire disease depends on early diagnostics. If it is timely, the treatment is less demanding and the patient handles it much better. In the area of therapy, the most frequent methods of treating this disease are illustrated. In connection with this disease, it is important to mention dispensarisation and prevention, oncological and palliative care as well as the impact of the disease on the patient's psychic condition. The bachelor thesis is complemented with supplements in the form of tables. One of them refers to incidence, mortality and prevalence of malignant melanoma from 1994 to 2014. Other tables serve for easier understanding of classification of skin tumours. The objective of the thesis was to map the nursing care of oncological diseases of skin. A nurse is an important element of prevention. She can be helpful and educate the population about self-monitoring of skin. During the treatment, the nurse applies medicaments and treats skin after surgical procedures or radiotherapy. The nurse plays an essential role within oncological and palliative care. That is why her relevance is described in several chapters. For many patients, the support of the nursing staff is a very important factor also in the area of psychic condition, on which the approach to the disease, as well as the development of the treatment, are based. It was necessary to acquire many findings about oncological diseases of skin to write this thesis. Many experts are engaged in this area nowadays, especially in the area of treatment. Information was obtained from both Czech and foreign expert bibliographic publications and articles. The studied information is incorporated into individual chapters that can be beneficial for anyone who is engaged in this specific area of oncology.

This article describes experimental measurements of a skin temperature in vicinity of a surgical wound. Measurements were carried out in collaboration with the orthopedic department of the University Hospital Olomouc, Czech Republic. A system used for sensing, transmission and visualization of physiological parameters was developed at the University of West Bohemia in Pilsen. A main goal of measurements was to evaluate technical parameters of the system, and also non-technical aspects associated with the use of the system. Technical parameters that was verified was a reliability of the system as a whole. This covers a temperature and motion sensing, a wireless transfer of measured data from sensors through wireless routers to the data server and data visualization in web application. To asses non-technical aspects of the system use, a sociological survey was carried out. Patients were asked to answer several questions related to the use of the system and temperature sensors. Conducted series of measurements and the survey showed that the vast majority of patients are accepting new technology positively. Measurements also verified the usability of the temperature sensor for local skin temperature measurement. Furthermore measurements revealed some issues associated with use of the sensor for monitoring of human body temperature and set a new target for further research and development.

Thesis deals with ultraviolet radiation on the human body, especially with solarium radiation. Theoretical part deals with history of solariums, effects of individual radiation for human and what are their advantages and disadvantages. It relates with body skin and that is why there are informations about that too. The theoretical part is followed by practical part for which I created a survey by my own. It helps me find out for example what people know about effects of UV radiation for human organism or how often they visit solar studio. I processed the results of the survey directly from solar studio Ergoline´s customers and online survey created on the internet. From total 60 asked people, 58 % which means 35 of them feel mentally improvement after sunbathing and 42 % of them visiting solarium for esthetical reasons.

Work maps tailored health students from the harmful effects of UV radiation and traces their knowledge about the effects of UV radiation on the body. The cohort consisted of students and combined form of Health and Social Studies University of South Bohemia in Ceske Budejovice. The aim of this work is to identify students' knowledge of ZSF JU. The effects of UV radiation on the human body and mapping the extent of protection of health of students of the ZSF JU from the harmful effects of UV radiation. The theoretical part describes the general characteristics of UV radiation and its effects on the human body. Work is more geared to actual problems of skin tumors, mainly the issues already mentioned malignant melanoma, which is currently the worldwide especially among the white population serious health problem. In this work he received a lot of attention because among all tumors occupies an important place mainly due to the increase in the number of cases in recent years, due to the very difficult to estimate prognosis and often very rapid pace. The theoretical part deals with the issues of prevention and alone and prevention programs that are reticent part of health care , and that can detect the disease at a stage when it is still treatable. Has a special position then the international program of the World Health Organization WHO Intersun that assesses and quantifies health risks and develop appropriate responses by issuing various directives, recommendations and dissemination of information, while providing practical advice and information to government health and environmental issues in UV radiation. Practical work logic device monitoring students' knowledge about the positive and negative effects of UV radiation on the body, while also monitoring their attitudes toward tanning , protection from sunlight and behavior in case of suspicious skin changes.

The task of the thesis is to design a new chair design with respect to current requirements for this type of furniture. The work is divided into two parts. In the first, theoretical part is at the beginning analyzed the history of design. The following chapters are focused on the theme of sitting, its negative consequences, alternative solutions and basic types of sitting positions, an overview of main dimensions, standards and materials that were used for the design. The second, practical part deals with the design process. At the start is compiled research focused mainly on the actual situation on the market and innovative design concepts of a chair. Work continues with finding own concept by sketches and 3D visualizations. The final phase is deticated to work on the selected concept, where are specified all details of the project from the dimensions and shape to manufacturing process and versions, including a fabrication of a model in 1: 4.

The aim of this work was to evaluate international trade with Manis. spp. according to the CITES database during the period 2002-2013. Manis spp. was classified in appendix II CITES in 1995. In 2000 there was an unsuccesfull attempt to reclassified Manis spp. to appendix I CITES. In this year the zero quota was set for Asian pangolins for commercial purpose. A rapid increase of an illegal trade was noticed after the setting of zero quota. The most frequent trade with pangolins was in these forms: 1) derivatives, 2) specimens, 3) scales, 4) live pangolins and 5) skins. Asian pangolins´ species were traded mostly in forms of derivatives, specimens, scales and skins in the period 2002-2013. M. javanica and M. pentadactyla with medium and large distribution area were quantitatively the most traded species, respectively. Main importers of Asian forms of pangolins were: USA (derivatives and specimens), China (scales in kg) and Indonesia (scales in pieces) and Mexico (skins). The African pangolin M. tricuspis was the third most frequent species in the international trade and was traded especially in form of live pangolins. M. tricuspis lives in one of the largest distribution area. Main importer of live pangolins was Italy. Breeding pangolins in captivity is due to the specific feeding requirements demanding. There are about 46 individuals of pangolins in Zoos all over the world, whereas in Europe there are only 2 pangolins in the Leipzig Zoo.

Tissue engineering is an emerging interdisciplinary field developing new ways of treatment of patient's tissue defects using artificial substitutes. Skin tissue engineering is developing skin substitutes and wound dressings that would replace current treatment using autologous, allogeneic or xenogenic substitutes. There are high demands on materials which should serve as a scaffolds for dermal fibroblasts and keratinocytes. They must be non-cytotoxic and biodegradable with a rate proportional to formation of a new tissue. The materials should support adhesion and proliferation of the cells and even they could release growth factors and antimicrobial substance to enhance healing and new tissue formation. In this master thesis, the cell adhesion and proliferation were evaluated on sodium carboxymethyl cellulose (Hcel® NaT), poly-ε-caprolactone (PCL), poly-L-lactide-co-ε-caprolactone (PLA/PCL) and cellulose acetate (AC) nanofiber membranes. Primary human dermal fibroblasts and HaCaT cell line keratinocytes were selected for evaluation. The cell adhesion was observed by fluorescent microscopy, the proliferation was determined by metabolic assay (WST-1) and the material cytotoxicity was evaluated in xCELLigence® system. Materials did not show cytotoxic effects on the cells. However, the materials did...