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Liver cell regeneration is an important biological process that allows mammals to maintain liver function while recovering from liver damage. Liver cell proliferation serves as the primary mode of liver regeneration, which in hepatocytes is activated by the transition from the G0 to G1 phase of the cell cycle. Proliferation is also promoted by non-parenchymal liver cells among which include Ito cells, Kupffer cells, and endothelial cells of hepatic sinusoids. In a comprehensive analysis of key signaling pathways, it was clearly demonstrated that the Wnt/β catenin, Notch, Hippo, NF-κB, and Hedgehog signaling pathways play a key role in the regulation of liver cell proliferation and differentiation during regeneration. The regenerative potential of the liver is influenced by various factors such as age, extent of damage and health conditions. Additionally, the remarkable regenerative capacity of the liver has clinical implications in the context of liver transplantation, partial hepatectomy and the treatment of liver diseases such as cirrhosis, hepatitis and hepatocellular or cholangiocellular carcinoma. Modulation of key signaling pathways and identification of novel molecular targets can improve the clinical outcomes of patients with liver diseases or even accelerate the entire process of liver...

v Abstract Author: Jan Pavlíček Title: A case report of physiotherapeutic treatment provided to a patient following liver transplantation and colon resection Objectives: This bachelor thesis aimed to introduce theoretical information concerning issues with a liver transplant, and large intestine resection and elaborate on a case report of a patient that underwent the above-mentioned procedures. Methods: This bachelor's thesis is divided into two parts. The liver and large intestine's anatomy, physiology, and pathophysiology are discussed in the first general section. Following chapters are devoted to the main treatment options for advanced liver diseases, i.e. liver transplantation, and indication and possibilities of colon resection. Physiotherapeutic approaches used in the mentioned procedures and further options of treatment for these patients are mentioned in the later chapters. The second specialized part describes a case report of a patient post-liver transplantation and large intestine resection. Included is a report of therapies that the patient underwent as part of ongoing professional practice in IKEM in January and the beginning of February 2023. Results: The patient was not showing any signs of improvement after the therapeutic interventions. This was due to many factors including the general...

Author: Barbora Mahenová Supervisor: Mgr. Michaela Stupková Title: Case report of physiotherapeutic care of an adult patient after liver transplantation Objectives: The main aim of this bachelor thesis is to summarize the theoretical information about liver transplantation and to prepare a detailed case report of an adult patient who underwent this surgery. Methods: The theoretical part summarizes the knowledge about liver transplantation, specifically about indications and contraindications for transplantation, waiting list, organ donation, complications of the procedure, immunosuppressive therapy and quality of life of patients after liver transplantation. Furthermore, the theoretical part covers the general principles of rehabilitation after transplantation and physiotherapeutic examination and therapeutic methods and procedures. The special part includes a detailed case study of physiotherapeutic care of a patient who has undergone liver transplantation. The case report also includes a description of the therapies that took place as part of a continuous professional practice at the Institute of Clinical and Experimental Medicine in Prague between January and February 2023. Results: The patient achieved an improvement in fitness and a significant reduction in pain in the surgical wound area over...

The circadian system influences almost all physiological processes in the mammalian body. Among other things, detoxication processes in the liver are under circadian control. Both central and peripheral clock in the liver regulate the expression of genes involved in the detoxication of xenobiotic substances and drugs. The first part of this thesis summarizes the main characteristics of the central and peripheral circadian clocks, including their molecular basis. The second part focuses on the main functions of liver tissue with a focus on detoxification processes. The emphasis of the third section is on circadian rhythms in the expression and activity of enzymes called cytochromes P450, which are the most important system catalyzing phase I detoxication, and also briefly discusses the influence of the circadian system on the expression of nuclear receptors and PAR bZIP transcription factors involved in the regulation of cytochrome P450 transcription. The last section describes the influence of the circadian system on the efficacy and toxicity of selected drugs, on paracetamol metabolism with the main purpose of describing how paracetamol-induced hepatotoxicity is affected by time of administration. Key words: circadian system, liver, detoxication, cytochrome, paracetamol

The present thesis investigated the impact of a ten-week oral exposure to BPS (50 ?g/kg live weight/day) in female outbred Wistar Han rats. The effect of BPS exposure on live weight, absolute and relative kidney and liver weights, morphometric parameters of kidney and renal corpuscles, histopathological changes of kidney and liver were evaluated. In addition, the concentration of total protein, triacylglycerols, cholesterol, urea and alanine aminotransferase activity were determined in blood serum.

Title: A Case Study of Physiotherapy on a Patient after Liver Transplantation Author: Lukas Hettiaratchi Objectives: The purpose of this work is to review in-depth theory concerning liver transplantations along with the rehabilitation process that entails it. Moreover, create an adequate case study of a patient after liver transplantation. Methods: This work is divided into two sections, the theoretical part, and the special part. The first part consists of information gathered through scientific literature, it includes the anatomy, physiology and pathophysiology of the liver. Furthermore, it dives into the specifics of liver transplantations and recommended physiotherapy. The second section exhibits a complete case study of early post-operative physiotherapy on a patient after liver transplantation. The case study was conducted during a month of clinical practice at Institut Klinické a Experimentální Medicíny (IKEM) from the 10/01/2022 - 04/02/2022. Results: The patient significantly progressed throughout the physiotherapeutic care. Their overall physical condition improved, that led to the ability of independent verticalization and gait. Keywords: Liver, transplantation, alcohol liver disease, portal hypertension, ascites, physiotherapy

The diploma thesis deals with endoglin, a transmembrane glycoprotein that can be cleaved to form so-called soluble endoglin, which then circulates in the blood. The theoretical part of the thesis is devoted to cardiovascular and liver diseases related to changes in the expression or level of endoglin in the blood and summarizes current knowledge about endoglin and its relation to various diseases. The experimental part of the thesis describes the selection of a suitable antibody against endoglin intended for western blot (WB). We have four anti-endoglin primary antibodies available from three manufacturers. The purpose was to test the antibodies and determine, which one had the best ability to detect endoglin in mouse tissue samples such as liver or aorta on WB. Affiblot was used to verify their ability to bind endoglin and also to compare their affinities to this protein. Newly developed patented dot-blot-based device. The binding of the used antibodies to endoglin was verified using the WB method and then the results of both methods were compared. The obtained results showed that the Abcam antibody has the most suitable parameters regarding specificity and sensitivity for the identification of endoglin in mouse tissue on WB. Keywords: endoglin, soluble endoglin, NASH, cirrhosis, non-alcoholic...

The liver is a very important parenchymatous organ for humans. Their function can be impaired by various influences. Common noxins include hepatotropic viruses and other microorganisms, alcohol and other toxins, including drugs, metabolic disorders, biliary obstruction, tumors, genetic abnormalities, and others. The work focuses on the comparison of histological images and clinical diagnosis of healthy individuals with steatosis and cirrhosis. The theoretical part describes the anatomy, physiology and histology of the liver with the perihepatic area. The next chapters focus on the clinic of steatosis and cirrhosis. The practical part describes the procedures for the preparation of healthy and affected liver preparations, as well as the histological methods used for staining individual cell structures. The result of comparing diagnoses of histological specimens and clinical diagnoses of individuals is 94.23 % agreement. Although the found discrepancy of diagnoses is small (5.77 %), it is a reason to think about the development and use of new or modified methods, including the creation of various calculators, including clinical data. Key words: histology, liver, steatosis, cirrhosis

Author: Kateřina Čemusová Title: A case study of physiotherapy treatment of a patient after ALPPS for leiomyosarcoma. Aims: This thesis aims to record physiotherapy care for the patient after undergoing ALPPS treatment for leiomyosarcoma, determination, management and recording of individual therapeutic units, and evaluation of their effect. Methods: The thesis consist of a theoretical and practical part. The theoretical part consist of general information about the composition and physiology of the liver. Leiomyosarcoma and other liver diseases are also described. Lastly, the ALPPS method is mentioned. All information is cited from academic literature and other professional sources. The practical part contains a case report of a patient, who has undergone ALPPS treatment for leiomyosarcoma. It also contains the record of individual therapies, that have been performed based on a kinesiological analysis. The analysis has been done as a part of a physiotherapy intership at the Institute of clinical and experimental medicine in Prague from 18th January to 12th February 2021. Results: The treatment and the effect of therapeutic care were significantly slowed down due to the division of the surgery into two stages. In general, the dysbalances of muscle strength and hypertonia have been corrected. The...

Bisphenol S (BPS) is an organic compound widely used in industrial production as a substitute for bisphenol A (BPA) for which negative impacts on the human body have been demonstrated and have therefore been significantly regulated. However, as time progresses, the negative properties of BPS are also shown, especially for the endocrine and reproductive systems. The liver is an organ in which xenobiotics are detoxified and thus BPS and BPA. The aim of the bachelor thesis was to verify whether 10-week oral exposure to BPS (4 g, 50 g, 100 mg/kg bw/day) and BPA (100 mg/kg bw/day) will lead to a negative impact on body weight, weight gain, weight and morphological liver structure and on the concentration of triglycerides, cholesterol, total white-vinas, urea and alkaline phosphatase activity in blood serum. The experiment involved 24 rats. BPA and BPS were administered daily by gastric tube in sunflower oil. The results of our research clearly did not show a negative effect of BPS and BPA in rats on body weight, absolute gain, absolute and relative liver weight in rats. There was also no evidence of histopathological changes in rat liver. In contrast, bio-chemical parameters were altered compared to the control group. Decreases in trigly-cerides, total protein content, urea, alkaline phosphatase activity and increases in serum cholesterol were noted. In the 50 g/kg body weight/day group of rats, the highest and lowest decreases in biochemical parameters were observed, respectively. The exposure dose of 100 mg BPS and BPA in our experiment did not cause liver histopathological changes or changes in biochemical parameters according to the hy-pothesis. A possible explanation could be given by the carrier for which sunflower oil with natural antioxidant content was used.

Tryptophanis anaminoacidwhichhasmanyfunctionsinthebody. Besidesitsparticipationin theproductionofproteins,itactsasasubstrateforthekynurenineandmethoxyindolemetabolic pathways. The kynurenine pathway ends with the production of nikotinamid e adenin dinukleotide ( NAD + ) ,whichisneededfortheproductionofcellularenergy. Thus,withincreased energy demand during immune system activation, the activity of the kynurenine pathway is increased. Dueto increasedactivity,itproduces more immunoactiveandneuroactivemetab olites suchaskynurenicacidandquinolinicacid.Thesemetabolitesareinvolvedinmanyprocessesin the body and affect the pathology of many diseases. Studies show that regulation of these metabolites could be a key innovation in the treatment of cance r, cardiovascular or neurodegenerative diseases. The methoxyindole pathway is another important tryptophan processingpathway.Itsbest - knownmetabolitesareserotonin,whichactsasaneurotransmitter, andmelatonin,ahormonewithimmunomodulatoryeffect sregulatedby thecircadianclock . This workdeals with thecircadian rhythmicityofenzymeexpressionofthesetwo metabolic pathways.Italsodescribestheeffectofsystemicadministrationoflipopolysaccharideendotoxin ontheexpressionofgenesoft heseenzymes.OurexperimentsusedWistarratsat30daysofage. The lipopolysaccharide was administered...

Charles University Faculty of Pharmacy in Hradec Králové Department of Pharmacology & Toxicology Student: Veronika Šulová Supervisor: doc. PharmDr. Lukáš Červený, Ph.D. External supervisor: pplk. doc. MUDr. Jaroslav Pejchal, Ph.D. et Ph.D. Title of diploma thesis: Changes of internal organs after percutaneous exposure to sulfur mustard Sulfur mustard is a chemical warfare agent belonging to the group of blistering agents. The theoretical section of the thesis is mainly focused on the description of acute toxic effects, the mechanism of action, and deals with the current possibilities of poisoning therapy. The experimental section is focused on monitoring the effect of sulfur mustard poisoning in the liver, lung, and kidney of female C57BL/6J mice after the percutaneous administration. This work aimed to evaluate markers of oxidative stress and histopathological changes of the selected organs at 3, 5, and 7 days after the poisoning. Ferric reducing antioxidant power (FRAP) and thiobarbituric acid reactive substances (TBARS) methods were used to determine markers of oxidative stress. Histopathological changes were evaluated microscopically using the hematoxylin-eosin staining method. The airness of the lung parenchyma was also assessed by computer image analysis. First, the LD50 of sulfur mustard was...

Author: Tereza Šťastná Title: Case study of physiotherapy treatment of a patient after liver transplantation Aims: The aim of this bachelor's thesis is to process theoretical knowledge about liver, liver transplantation and to perform a case report of a patient who underwent transplantation. Methods: This thesis is created on the basis of continuous monthly practise at the Institute of clinical and experimental medicine at the department of KARIP, which took place in the period from 6th to 31st January 2020. Thesis is divited on theoretical and practical part. In theoretical part are compiled informations about anatomy and physiology of liver, than about liver diseases and liver transplantation. In the end of this part are informations about physiotherapic care after liver transplantation. Practical part conteins case study of physiotherapy treatment of patient after liver transplantation and daily records of individual therapies. The bachelor thesis is written from the point of view of a physiotherapist. Results: The patient was in coma almost whole time of therapies, that fact greatly affects their process. The greatest success was increase of muscle strength and deepening of tidal volume during therapies. In the last days of practice, the patient's health deteriorated rapidly and unfortunately...

Author: Veronika Sosnovcová Title: The case study of physiotherapy treatment of a patient after liver transplantation Objectives: The main aim of this bachelor thesis is to elaborate theoretical knowledge of liver transplantation and to introduce the case study of physiotherapeutic treatment of the patient who has undergone a liver transplant. Methods: The bachelor thesis consists of two parts. The first part is the theoretical part, which summarizes basic information about anatomy, physiology and patology of the liver. The next chapter is about one of the main treatment options in advanced liver disease, what is the liver transplantation. Physiotherapeutic procedures that can improve patient's condition are mentioned in the last chapter of the theoretical part. The special part describes the case study of the patient after liver transplantation. It also includes the record of individual therapies, which was applied during the practice in IKEM in January 2020. Results: The patient improved after the therapies. The most important progress was that patient could walk unassisted along the corridor and even up and down the stairs. The patient became completely independent and was discharged home. Keywords: liver, transplantation, cirrhosis of the liver, physiotherapy

Title: The case study of physiotherapy treatment of a patient after liver transplantation. Author: Jana Švejdová Head of the thesis: Mgr. Michaela Stupková Objectives: The aim of this bachelor thesis is to summarize the facts about liver transplantation including an example of physiotherapeutic treatment on a specific patient after liver transplantation. The thesis is written from a physiotherapeutical point of view. Methods: The bachelor thesis consists of two parts - theoretical part and special part. The theoretical part contains information about anatomy, physiology and pathology of the liver, including liver diseases, description of the transplantation process and physiotherapeutic techniques used after the surgery. All of this information was obtained from scientific literature. The special part of this thesis describes the case study of a specific patient after undergoing liver transplantation including a description of physiotherapeutic care, examinations and therapeutic units. This part was created during my practice in the Institute of Clinical and Experimental Medicine in Prague between 6th and 31st January 2019. Results: The main improvement was in muscle strenght and overal physical condition being noticeable mostly in the gait ability and activities of daily living. Keywords: liver,...

"Vitamin D blood level, factors that affect it, and its relationship to reccurent infections" is topic repeatedly discussed in domestic and foreign literature. This topic is a multidisciplinary issue, but it mainly relates to nutrition and sun exposure. The aim of this study is to map relationship of vitamin blood levels in relation to diseases of the respiratory system, vitamin D suplementation, recurrent infections, usage of sunscreen with a protective factor, nutrition and outdoor stay in winter months. The study was conducted on a research sample of preschool children. Two sets of data were used to evaluate objectives and research questions. The first research group consisted of 48 children and had 2 groups. The first group of 21 children was established in the Waldorf kindergarten in Rudolfov and the second group of 17 children was created in a non-state medical facility in Trebon. The first research consisted of 48 children who were tested on vitamin D blood level and filled survey questionnaire. The second research consisted of 61 children who were tested on vitamin D blood level in a non-state medical facility. Some results of this study are contradictory because of inadequate methodology. Positive results have been shown with vitamin D supplementation, higher consumption of liver, eggs and daily intake of vegetable oils.

Title: The case study of physiotherapy treatment of a patient after liver transplantation. Author: Veronika Jelínková Head of the thesis: Doc. PaeDr. Dagmar Pavlů, Csc. Objectives: The aim of this bachelor thesis is to summarize the theoretical knowledge about the issue: liver transplantation and include an example of physiotherapeutic treatment on specific case of a patient after liver transplantation. The thesis is written from a physiotherapeutical point of view. Methods: The bachelor thesis consists of two parts - theoretical part and special part. The theoretical part contains information about the anatomy, physiology and pathology of the liver, including liver diseases, defect of the liver function and clinical presentation. All of this information was obtained from scientific literature. The special part of this thesis describes the case study of specific patient after liver transplantation. This part was created during my practice in the Institute of Clinical and Experimental Medicine in Prague between 7th January 2019 and 1st February and includes a description of physiotherapeutic care, examinations and therapeutic units.. Results: During therapies, the patient mainly improved muscle strength and overall physical condition. She was able to rise to sitting position without assistance of...

Use the contrast-enhanced ultrasound in the monitoring of the effect of liver tumors treatment by radiofrequency ablation. Summary High quality imaging is crucial for the treatment of liver tumors by the percutaneous radiofrequency ablation. Imaging methods are used for planning, navigation of the intervention and monitoring after treatment. An early detection of residual tumor tissue or recurrence affect significantly the quality of life and life expectancy of the patients. Contrast enhanced ultrasound (CEUS) seems to be a suitable method for monitoring the locoregional treatment mainly because zero radiation impact on patient and allows the highly accurate real-time assessment of vascularization. The aim of my work was to verify the ability of CEUS to detect residual tumor tissue or tumor recurrence in the field of changes after radiofrequency ablation. We evaluated the group of 73 patients, who were treated by radiofrequency ablation (RFA) for liver cancer. CEUS reached in our group the overal sensitivity 77.27 %, and in the group of colorectal cancer metastases 83.33 %. We compared CEUS with results of computed tomography (CT), which is the most commonly used method for monitoring the treatment. CEUS and CT results are similar. Based on this study, the CEUS appears to be a suitable method for monitoring...

Title: Case of physiotherapy care of a patient after liver transplantation Aims: The aim of this bachelor thesis is to summarize the theoretical knowledge about liver transplantation and to introduce a possible example of physiotherapeutic care for a particular case report of a patient after liver transplantation. Methodology: The bachelor thesis is divided into two parts: theoretical and special. The theoretical part contains general knowledge of liver anatomy and physiology, problems of pathophysiology, transplantation and physiotherapeutic procedures and methods concerning liver transplantation. All information in the theoretical part was taken from the literature. A special part of this bachelor thesis was processed on the basis of continuous professional practice in the period 7. 1. 2019 - 1. 2. 2019 in IKEM Prague. It is processed as a case report of a certain patient. It contains input kinesiological analysis, recording of the course of therapies, kinesiological analysis and evaluation of the effect of therapy. Results: During the therapy, the patient experienced a shift in general health. At the beginning it was a patient with a qualitative impairment of consciousness without any cooperation on therapy, and with disgraceful time her condition was adjusted in such a way that she could...

Author: Marika Šepsová Title: Genetic diseases of the liver Form: Bachelor Thesis University: Charles University, Faculty of Pharmacy in Hradec Králové Degree: Medical Laboratory Technician Liver function may be affected by various factors including genetic diseases. The aim of this bachelor thesis is to collect and summarize current information about genetic diseases of the liver. Diseases with the highest incidence in the population are Dubin-Johnson syndrome, Rotor syndrome, Crigler-Najjar syndrome and Gilbert's syndrome. They are known as inherited hyperbilirubinemia characterized by an impairment in bilirubin metabolism. These genetic diseases are very rare with exception of Gilbert's syndrome. However, despite low prevalence and incidence it is necessary not to prolong their diagnosis. Most of them do not have any complications and do not require any treatment. The exception is Crigler-Najjar's syndrome, as untreated can have fatal consequences. Wilson's disease and hereditary hemochromatosis are inherited disorders of metal metabolism. Wilson's disease is a rare disease caused by an impairment in copper metabolism. Inherited hemochromatosis causes excessive iron deposition in the liver and other organs. Alpha-1 antitrypsin deficiency is characterized by impaired alpha-1 antitrypsin protein...

Title: Case study of physiotherapy care of a polymorbid patient after liver transplantation Objectives: The aim of this work is a conclusion of theoretical knowledge about physioterapeutic care after the liver transplantation, systematic leading and recording of terapeutic units, tracking the results and overal apreciation of all proposed therapies of polymorbid patient after liver transplantation. Methods: This thesis is split into two parts, first part is research of resources about given topic, second part is case report of polymorbid pacient after the liver transplantation with whom I worked in the time of 18.1. 2019 - 30.1. 2019 in IKEM in Prague. First theoretical part contains anatomical and fyziologial description of liver and gallbladder, types of illnesses that affects these organs, the diagnostics of those illnesses and treatment. Second - special - part of this thesis is case report of the patient. Results: Due to serious condition of the patient was the main goal of the physioterapeutic care to keep the biggest possible condition and improve actual physical state of the patient after frequent revision operations. These goals were accomplished in the highest possible degree, after the termination of therapies, is the patient able to breath spontaneously, and partly able to self-help by...

1 Abstract In this thesis I have focused on the role of lipids in the pathogenesis of liver diseases, specifically on cholestasis and non-alcoholic fatty liver disease (NAFLD). The first major aim was to clarify the changes in liver ganglioside metabolism in various types of cholestasis and to elucidate the role of heme oxygenase-1 (HMOX1) and associated oxidative stress. The second objective was to determine the effects of n-3 polyunsaturated fatty acids (n-3 PUFA) administration on NAFLD development in a rodent dietary model of NAFLD and in patients with metabolic syndrome and NAFLD. Our results suggest that increased ganglioside biosynthesis and their re-distribution might represent a general protective mechanism of hepatocytes under cholestatic conditions (both estrogen-induced and obstructive aetiology). These changes are closely related to oxidative stress and might protect hepatocytes against deleterious effect of accumulated bile acids. The lack of HMOX1 activity and subsequent oxidative stress potentiate pathological changes in the liver and resulted in tissue-specific modulation of synthesis and re-distribution of gangliosides (in vivo and in vitro). Contrary to it, HMOX1 activation has an opposite effect and may represent a general hepatoprotective mechanism. We have proven that observed changes...

Author: Nikola Jozefčeková Title: Endoglin and liver Form: Diploma Thesis University: Charles University, Faculty of Pharmacy in Hradec Králové Degree: Pharmacy This diploma thesis concludes the available information about endoglin, its isoforms in the liver and its impact on the liver during various pathological conditions. In the first part of the thesis describes morphology and physiology of the liver, its structure, histology and metabolic functions. Second part contains an information about endoglin, its isoforms and role in TGF-β signaling complex, expression of endoglin and regulation of the expression. In the third part are described liver diseases during which is expression of endoglin changed. This part deals with the significance of endoglin as a diagnostic and prognostic marker. Endoglin (CD105, TGF-β receptor III) is a homodimeric transmembrane glycoprotein and a membrane co-receptor TGF-β with high expression in endothelial cells. Endoglin plays an important role in the vascular development. Endoglin is involved in the processes of angiogenesis, vascular homeostasis and TGF-β signalization. It affects activity of TGF-βRII, ALK1 and ALK5 receptors. Due to the interactions with TGF-β complex, modulation of activity of ALK receptors and Smads, endoglin controls fibrotic and anti-...

The aim of this thesis was to evaluate the examined samples of liver tissue to find out the amount of aflatoxin in deer game. A total of 221 samples were examined for analysis purposes using the RIA method. Samples were obtained from dead and captured heads of deer game coming from private game reserves and game reserves belonging to the Forests of the Czech Republic. Samples of deer game were divided into groups according to their types, sex, and age. Sex was determined visually, and age via the development and grinding of the teeth. The highest concentration of B1 aflatoxin in liver was measured in a 6 year-old dead female (7,39 g.kg-1). According to the statistical evaluation (P

The aim of this thesis is to evaluate the influence of the seeds cakes of milk thistle (Silybum marianum L.) into feed fodder of laboratory rats. Milk thistle is one of the most famous and most frequently used herb positively influencing liver function. The aim was to verify the hypothesis that the seeds cakes of milk thistle in the feed fodder have influence on the biochemical parameters of blood. There were 24 male laboratory rats of Wistar albino species separated into 2 groups (A, B). Rats in the group A were fed with feed fodder with additional 10% of milk thistle seeds cakes. The control group (B) was fed with feed fodder without any additional milk thistle seeds cakes. The content of silimarin in the seeds cakes was 26,5mg/g. During the 30 days experiment average weight increase and biochemical values of blood was observed. Weight was measured on the 0. - 7. - 14. - 21. – 30. day of the experiment. Blood measurements were conducted by heart punction after killing 3 laboratory rats by inhalative anesthesia of Isofuran on 0. - 10. - 20. - 30. day of the experiment. The full blood sample was collected to determine biochemical blood parameters. There was no significant influence of milk thistle seeds cakes on the biochemical parameters of blood. Group fed with milk thistle seeds cakes showed higher activity of AST enzymes as well as higher concentration of HDL laboratory rats fed with milk thistle seeds cakes. Laboratory rats fed with milk thistle seeds cakes showed higher increase in daily weight measurements than the group of control. Results show that milk thistle seeds cakes have no significant influence on health indicators taken from the biochemical parameters of the blood.

The aim of this bachelor thesis is to compare the food intake in patients with metabolic syndrome, who suffer from non-alcoholic fatty liver disease (NAFLD) or non-alcoholic steatohepatitis (NASH). The main aim of this bachelor thesis is to find out differences in the diet of different groups of patients comprising patients with simple obesity, patients with metabolic syndrome without steatosis, patients with metabolic syndrome with steatosis and patients with metabolic syndrome complicated by steatohepatitis. This thesis is divided into two parts - theoretical and practical. The theoretical part introduces metabolic syndrome, its definition, occurrence, etiology, complications and its prevention and treatment. The topics about pathophysiology of the liver and the definition of non-alcoholic fatty liver disease or non-alcoholic steatohepatitis are also covered. Methods: The practical part is a quantitative research in the form of questionnaires made at the 4th Internal Department at the General University Hospital in Prague. The patients met the criteria of the mentioned symptoms or diseases. The thesis compares the data from literature with the responses of the patients. We explored the question of the diet composed for example by regular intake of fermented milk products, selenium, green tea or...

Title: Case study of physiotherapy care of a patient after liver transplantation. Aims: The aim of this bachelor thesis is to summarize the theoretical knowledge about the issue of liver transplantation and to present an example of physiotherapeutic care on specific case of a patient after liver transplantation. Methodology: This thesis is divided into theoretical and professional part. The theoretical part contains information about the anatomy, physiology and pathologic physiology of the liver, liver transplantation and physiotherapy treatment after liver transplantation. These information were obtained from the scientific sources. Professional part was created as a research with case study during my professional experience in the Institute of Clinical and Experimental Medicine in Prague between 8 January 2018 and 2 February 2018, where I worked with a patient hospitalized after liver transplantation. It is processed as a case study of physiotherapeutic care, including physical examination, recording of therapies and evaluation of the effect of therapies. Results: The patient mainly improved muscle strength, respiratory stereotype and overall physical condition, increased range of motion and improved joint play of some small joints. During the last therapeutic units, the patient was able to do a...

Při nádorovém onemocnění jater musí chirurg rozhodnout, kudy nejvhodněji vést řez, a je nucen určit odhad množství zbylé části jater. To je důležité pro následné stanovení funkční rezervy ponechaného jaterního parenchymu. Práce vznikla z běžícího projektu LISA na Katedře kybernetiky FAV ZČU ve spolupráci s Lékařskou fakultou Karlovy Univerzity v Plzni a Biomedicínského centra. Cílem je navrhnout počítačovou podporu, která pomůže chirurgům zvolit správnou léčebnou proceduru. Navrhnuté rozhraní bude realizováno formou webové stránky a bude zobrazitelné na různých platformách.

The term "biologically active compounds" covers a wide range of substances originating from natural or synthetic origins. These compounds can enter the aquatic environment through wastewater treatment plants, manufactural discharge or they are naturally present in the aquatic plants or microorganisms. Their adverse effects on fish has been widely studied and accepted. This thesis is focussed on the adverse effect of several bioactive compounds (i.e. pharmaceuticals, phytochemicals, or complex mixture of pollutants) on fish using different experimental design as in vitro, in vivo and in situ. In the first part of this thesis, in vitro effects of pharmaceuticals and phytochemicals or their combinations on fish CYP system were investigated. Moreover, effects of standard carrier solvents used in enzyme activity assays were also investigated. An important finding in this study was the lack of effects of either dexamethasone, quercetin, or indole-3-carbinol on EROD activity; however, when these agents were combined, EROD activity was strongly inhibited. This demonstrates that combination of compounds might exert different effects than single compounds, and the effects of mixture compounds cannot be predicted from the effects of individual compounds. In the second part of the thesis, the effects of chronic exposures of rainbow trout to dexamethasone and clotrimazole were investigated. The results regarding dexamethasone showed similarities with in vitro studies, and both in vitro and in vivo exposure of dexamethasone did not alter the CYP enzyme activities. On the other hand, in vivo exposure of clotrimazole yielded conflicting results with findings from the in vitro studies. Clotrimazole induced EROD activity in chronically exposed rainbow trout, and BFCOD activity showed biphasic pattern in which it was inhibited at environmentally relevant concentrations and induced at high concentrations. Thus, the observed effects suggest that clotrimazole could negatively affect fish CYPs at environmentally relevant concentrations. However, in the environment, the effects of clotrimazole and dexamethasone on fish CYPs system might be modified because of unknown compounds in these mixtures. Therefore, further investigations were done to identify the effects of mixture compounds using an in situ model. The last part of the dissertation addresses the effects of cocktail PPCPs on common carp under natural conditions. In situ studies provide valuable information on both hepatic and intestinal CYP activities. Both EROD and BFCOD activities were affected by the PPCPs that are present in the exposed fish. Moreover, changes in intestinal CYP activities suggest that fish can ingest some of these contaminants through their feed. Therefore, the intestines might be responsible for elimination of some of these pollutants and act as the first barrier of pollutant entry in fish. Despite the extensive studies concerning aquatic pollution, further studies are necessary. Development of new pharmaceuticals, their occurrence in the aquatic environment, and their effects on non-target organisms should be continuously monitored.

Campylobacteriosis is according to European Food Safety Authority one of the most advanced worldwide foodborne disease causing gastroenteritis. The Czech republic ranks at number one in the highest incidence of campylobacteriosis. This thesis deals with the determination of thermotolerant Campylobacter spp. in samples of poultry and pork liver, in chicken and raw milk. From total number of 50 samples were 31 samples positive for Campylobacter spp. For poultry liver and chicken at the back of thighs were 92 % of positive samples. For pig liver was lower incidence, only 46 %. For fresh raw milk was the lowest incidence of just 17 %. Higher incidence in the Czech republic reflects good way of searching their subsequent reporting infections.

The consumption of dietary supplements such as flavonoids may reduce risk of many civilization diseases. Flavonoids are able to modulate the activity of cytochromes P450 (CYPs), xenobiotic-metabolising phase I enzymes of biotransformation that are involved in the activation and detoxification of food-derived carcinogens. Inhibition of CYP activities by flavonoids has been extensively studied because of their potential use as agents blocking the initiation stage of carcinogenesis. On the other hand, flavonoids have been shown to enhance the activation of carcinogens and/or influence their metabolism via induction of specific CYPs. In the first part of this study, flavonoids dihydromyricetin and α-napthoflavone were explored for their possible effects on CYP1A1 expression and activity when administered in combination with carcinogen benzo[a]pyrene (BaP). For this purpose, liver, small intestine and colon microsomal fractions were isolated from treated rats and induction of CYP1A1 was evaluated by immunodetection and EROD activity measurements. In liver and small intestine, all combinations of BaP and flavonoids led to strong induction of CYP1A1 expression. Moreover, the CYP1A1 protein levels were almost identical to levels observed when the rats were treated with BaP alone. However, in comparison...