4,622 results on '"intestinal inflammation"'
Search Results
2. Use of GI BIOTICS 100B UFC to Improve Intestinal Health in Older Adults (GIBIOTICS)
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Borja Martínez Tellez, Principal Investigator
- Published
- 2024
3. Abelmoschus manihot polysaccharide fortifies intestinal mucus barrier to alleviate intestinal inflammation by modulating Akkermansia muciniphila abundance.
- Author
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Wang, Yumeng, Li, Chengxi, Li, Jianping, Zhang, Shu, Zhang, Qinyu, Duan, Jinao, and Guo, Jianming
- Abstract
The intestinal mucus barrier is an important line of defense against gut pathogens. Damage to this barrier brings bacteria into close contact with the epithelium, leading to intestinal inflammation. Therefore, its restoration is a promising strategy for alleviating intestinal inflammation. This study showed that Abelmoschus manihot polysaccharide (AMP) fortifies the intestinal mucus barrier by increasing mucus production, which plays a crucial role in the AMP-mediated amelioration of colitis. IL-10-deficient mouse models demonstrated that the effect of AMP on mucus production is dependent on IL-10. Moreover, bacterial depletion and replenishment confirmed that the effects of AMP on IL-10 secretion and mucus production were mediated by Akkermansia muciniphila. These findings suggest that plant polysaccharides fortify the intestinal mucus barrier by maintaining homeostasis in the gut microbiota. This demonstrates that targeting mucus barrier is a promising strategy for treating intestinal inflammation. Herbal-derived polysaccharide Abelmoschus manihot polysaccharide (AMP) fortifies intestinal mucus barrier by maintaining A. muciniphila homeostasis. [Display omitted] [ABSTRACT FROM AUTHOR]
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- 2024
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4. Gut microbiota dysbiosis contributes to α-synuclein-related pathology associated with C/EBPβ/AEP signaling activation in a mouse model of Parkinson's disease.
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Xiaoli Fang, Sha Liu, Muhammad, Bilal, Mingxuan Zheng, Xing Ge, Yan Xu, Shu Kan, Yang Zhang, Yinghua Yu, Kuiyang Zheng, Deqin Geng, and Chun-Feng Liu
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- 2024
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5. Integration of Gut Mycobiota and Oxidative Stress to Decipher the Roles of C-Type Lectin Receptors in Inflammatory Bowel Diseases.
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Yang, Liu, Hu, Min, and Shao, Jing
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INFLAMMATORY bowel diseases , *PATTERN perception receptors , *OXIDATIVE stress , *IMMUNE recognition , *THERAPEUTICS - Abstract
BackgroundMethods and ResultsConclusionUlcerative colitis (UC) and Crohn’s disease (CD) are two subtypes of inflammatory bowel disease (IBD) with rapidly increased incidence worldwide. Although multiple factors contribute to the occurrence and progression of IBD, the role of intestinal fungal species (gut mycobiota) in regulating the severity of these conditions has been increasingly recognized. C-type lectin receptors (CLRs) on hematopoietic cells, including Dectin-1, Dectin-2, Dectin-3, Mincle and DC-SIGN, are a group of pattern recognition receptors (PRRs) that primarily recognize fungi and mediate defense responses, such as oxidative stress. Recent studies have demonstrated the indispensable role of CLRs in protecting the colon from intestinal inflammation and mucosal damage.This review provides a comprehensive overview of the role of CLRs in the pathogenesis of IBD. Given the significant impact of mycobiota and oxidative stress in IBD, this review also discusses recent advancements in understanding how these factors exacerbate or ameliorate IBD. Furthermore, the latest developments in CLR-guided IBD therapy are examined to highlight the modulation of CLRs in fungal recognition and oxidative burst during the IBD process.This review emphasizes the importance of CLRs in IBD, offering new perspectives on the etiology and therapeutic approaches for this disease. [ABSTRACT FROM AUTHOR]
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- 2024
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6. Siraitia grosvenorii Extract Protects Lipopolysaccharide-Induced Intestinal Inflammation in Mice via Promoting M2 Macrophage Polarization.
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Wu, Huining, Guo, Mengru, Zhao, Linlu, Zhang, Jin, He, Jieyi, Xu, Anning, Yu, Zhichao, Ma, Xingbin, Yong, Yanhong, Li, Youquan, Ju, Xianghong, and Liu, Xiaoxi
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PROTEIN expression , *INFLAMMATION , *LIPOPOLYSACCHARIDES , *INTESTINES , *MACROPHAGES - Abstract
Siraitia grosvenorii has anti-inflammatory, antioxidant, and immune-regulating effects, while macrophages play an important role in reducing inflammation. However, it is still unclear whether Siraitia grosvenorii extract (SGE) is effective in reducing inflammation by regulating macrophages. This study investigated the regulatory effect of SGE on macrophage polarization in a lipopolysaccharide (LPS)-induced intestinal inflammation model after establishing the model in vitro and in vivo. The results from the in vivo model showed that, compared with the LPS group, SGE significantly improved ileal morphology, restored the ileal mucosal barrier, and reduced intestinal and systemic inflammation by increasing CD206 and reducing iNOS proteins. In the in vitro model, compared with the LPS group, SGE significantly reduced the expression of iNOS protein and cytokines (TNF-α, IL-1β, and IFN-γ) while significantly increasing the protein expression of CD206 in RAW264.7 cells. In conclusion, SGE can alleviate intestinal inflammation, protect the mucus barrier, and block the systemic immunosuppressive response by increasing M2 macrophages. [ABSTRACT FROM AUTHOR]
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- 2024
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7. Chicken Meal as a Fishmeal Substitute: Effects on Growth, Antioxidants, and Digestive Enzymes in Lithobates catesbeianus.
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Zhu, Bo, Xu, Wenjie, Dai, Zhenyan, Shao, Chuang, Hu, Yi, and Chen, Kaijian
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BULLFROG , *GROWTH disorders , *SUSTAINABLE aquaculture , *FISH meal , *CHICKENS , *DIGESTIVE enzymes - Abstract
Simple Summary: Fishmeal is a vital protein in aquafeed; however, its high cost and limited availability pose significant challenges. This study investigates the efficacy of chicken meal, an animal protein with a higher yield and lower cost, as a substitute for fishmeal. After feeding bullfrogs with different feed formulations for eight weeks, it was found that replacing 50% of fishmeal with chicken meal increased the survival rate of bullfrogs, thus enhancing their overall weight. Additionally, this partial substitution improved the antioxidant capacity and intestinal digestive enzyme activity of the bullfrogs. Conversely, a 100% replacement of fishmeal showed no significant effect on survival rate and resulted in reduced weight gain and lower amino acid content in muscle tissues, indicating that complete replacement is unsuitable. In pursuit of sustainable aquaculture, this study was performed to evaluate chicken meal as a substitute for fishmeal in bullfrog diets. Three experimental groups were established: a control group (FM) with 20% fishmeal, a CM50 group with 50% replacement (10% fishmeal), and a CM100 group with 100% replacement (0 fishmeal). Bullfrogs were fed for 56 days. The CM50 group exhibited significant increases in total weight gain and survival rate and a notable decrease in feed coefficient (p < 0.05). However, the CM100 group showed contrary effects. Increasing chicken meal substitution correlated with decreased amino acid content in muscle. Notably, the CM50 group demonstrated enhanced activities of antioxidant enzymes (CAT, T-AOC) and elevated gene expression levels (cat, sod, gst, etc.) in muscle and the intestine (p < 0.05), improved intestinal morphology, enhanced digestive enzyme activities (amylase, lipase), and reduced expression of inflammatory factors (il-1β, il-8, il-17, etc.). Conversely, the CM100 group's indicators regressed to levels similar to or worse than those of the FM group. Therefore, a 50% substitution of fishmeal with chicken meal effectively promoted bullfrog survival, protected the intestines, and enhanced antioxidant capacity, supporting its potential as a fishmeal alternative. However, the adverse outcomes of the CM100 strategy, including growth retardation and reduced amino acid content in muscle, indicate that complete replacement is unsuitable. [ABSTRACT FROM AUTHOR]
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- 2024
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8. The Impact of Non-Dysentery Shigella Infection on the Growth and Health of Children over Time (INSIGHT)—A Prospective Case–Control Study Protocol.
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Chakraborty, Subhra, Dash, Sampa, Antara, Nowrin Akbar, Roy, Bharati Rani, Mamun, Shamim Al, Ali, Mohammad, Naz, Farina, Johura, Fatema-Tuz, Lewis, Jade, Afroze, Farzana, Hasan, ABM Ali, Sack, David A., Ram, Malathi, Tofail, Fahmida, Ahmed, Tahmeed, and Faruque, A. S. G.
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SHIGELLOSIS ,RAPID diagnostic tests ,HOSPITAL care of children ,DYSENTERY ,COGNITIVE development - Abstract
(1) Shigella spp. (Shigella) is known for causing dysentery with blood in stool, but most children infected with Shigella have non-dysentery Shigella-associated diarrhea (NDSD). The World Health Organization recommends the use of antibiotics when diarrhea is bloody, leaving most NDSD cases untreated. The absence of dysentery may not indicate a low risk of death and severe morbidity. Rapid diagnosis and treatment of shigellosis in vulnerable, young children may be lifesaving. INSIGHT aims to determine the potential benefit of identifying NDSD cases (n = 296) and their outcomes compared to cases of Shigella dysentery [DS (n = 148)] and non-Shigella watery diarrhea [WD (n = 148)]. (2) Children seeking care at hospitals in Bangladesh will be enrolled and followed for 1 year (NDSD and DS) or 90 days (WD). We will determine the impact of NDSD on morbidity, mortality, gut health, nutritional status, and cognitive development compared to DS and WD in children and whether the simple "Rapid LAMP-based Diagnostic Test (RLDT)" can accelerate the detection and treatment of shigellosis in the clinical settings of rural Bangladesh. (3) INSIGHT will determine the impact of NDSD in children and determine if the treatment guidelines of shigellosis need to be revisited to improve clinical outcomes and the development of these children. [ABSTRACT FROM AUTHOR]
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- 2024
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9. Characterization of idiopathic chronic diarrhea and associated intestinal inflammation and preliminary observations of effects of vagal nerve stimulation in a non‐human primate.
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Populin, Luis C., Rajala, Abigail Z., Matkowskyj, Kristina A., Saha, Sumona, Zeng, Weifeng, Christian, Bradley, McVea, Andrew, Tay, Emmy Xue, Mueller, Ellie M., Malone, Margaret E., Brust‐Mascher, Ingrid, McMillan, Alan B., Ludwig, Kip A., Suminski, Aaron J., Reardon, Colin, and Furness, John B.
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VAGUS nerve stimulation , *INFLAMMATORY bowel diseases , *IDIOPATHIC diseases , *IRRITABLE colon , *DIARRHEA , *VAGUS nerve - Abstract
Background Methods Key Results Conclusions and Inferences Diarrhea is commonly associated with irritable bowel syndrome, inflammatory bowel disease, microscopic colitis, and other gastrointestinal dysfunctions. Spontaneously occurring idiopathic chronic diarrhea is frequent in rhesus macaques, but has not been used as a model for the investigation of diarrhea or its treatment. We characterized this condition and present preliminary data demonstrating that left vagal nerve stimulation provides relief.Stool consistency scores were followed for up to 12 years. Inflammation was assessed by plasma C‐reactive protein, [18F]fluorodeoxyglucose (FDG) uptake, measured by positron emission tomography (PET), multiplex T cell localization, endoscopy and histology. The vagus was stimulated for 9 weeks in conscious macaques, using fully implanted electrodes, under wireless control.Macaques exhibited recurrent periods of diarrhea for up to 12 years, and signs of inflammation: elevated plasma C‐reactive protein, increased bowel FDG uptake and increased mucosal T helper1 T‐cells. The colon and distal ileum were endoscopically normal, and histology revealed mild colonic inflammation. Application of vagal nerve stimulation to conscious macaques (10 Hz, 30 s every 3 h; 24 h a day for 9 weeks) significantly reduced severity of diarrhea and also reduced inflammation, as measured by FDG uptake and C‐reactive protein.These macaques exhibit spontaneously occurring diarrhea with intestinal inflammation that can be reduced by VNS. The data demonstrate the utility of this naturally occurring primate model to study the physiology and treatments for chronic diarrhea and the neural control circuits influencing diarrhea and inflammation that are not accessible in human subjects. [ABSTRACT FROM AUTHOR]
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- 2024
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10. Impact of Microbiota and Metabolites on Intestinal Integrity and Inflammation in Severe Obesity.
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Custers, Emma, Vreeken, Debby, Schuren, Frank, van den Broek, Tim J., van Dongen, Lieke, Geenen, Bram, de Blaauw, Ivo, Wiesmann, Maximilian, Hazebroek, Eric J., Kleemann, Robert, and Kiliaan, Amanda J.
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CYTOTOXIC T cells , *GUT microbiome , *TIGHT junctions , *CELL junctions , *MAST cells - Abstract
Obesity is a multifactorial disease associated with low-grade inflammation. The gut is thought to be involved in obesity-related inflammation, as it is continuously exposed to antigens from food, microbiota and metabolites. However, the exact underlying mechanisms are still unknown. Therefore, we examined the relation between gut pathology, microbiota, its metabolites and cytokines in adults with severe obesity. Individuals eligible for bariatric surgery were included. Fecal and plasma samples were collected at surgery timepoint, to assess microbiota and metabolite composition. Jejunal biopsies were collected during surgery and stained for cytotoxic T cells, macrophages, mast cells and tight junction component zonula occludens-1. Based on these stainings, the cohort was divided into four groups: high versus low intestinal inflammation and high versus low intestinal integrity. We found no significant differences in microbiota diversity between groups, nor for individual bacterial species. No significant differences in metabolites were observed between the intestinal inflammatory groups. However, some metabolites and cytokines differed between the intestinal integrity groups. Higher plasma levels of interleukin-8 and tauro-chenodeoxycholic acid were found, whereas isovaleric acid and acetic acid were lower in the high intestinal integrity group. As the results were very subtle, we suggest that our cohort shows very early and minor intestinal pathology. [ABSTRACT FROM AUTHOR]
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- 2024
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11. Intestinal microbiome and metabolome signatures in patients with chronic granulomatous disease.
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Chandrasekaran, Prabha, Han, Yu, Zerbe, Christa, Heller, Theo, DeRavin, Suk, Kreuzberg, Samantha, Marciano, Beatriz, Siu, Yik, Jones, Drew, Abraham, Roshini, Stephens, Michael, Tsou, Amy, Snapper, Scott, Conlan, Sean, Subramanian, Poorani, Quinones, Mariam, Grou, Caroline, Calderon, Virginie, Deming, Clayton, Leiding, Jennifer, Arnold, Danielle, Logan, Brent, Griffith, Linda, Petrovic, Aleksandra, Mousallem, Talal, Kapoor, Neena, Heimall, Jennifer, Barnum, Jessie, Kapadia, Malika, Wright, Nicola, Rayes, Ahmad, Chandra, Sharat, Broglie, Larisa, Chellapandian, Deepak, Deal, Christin, Grunebaum, Eyal, Lim, Stephanie, Mallhi, Kanwaldeep, Marsh, Rebecca, Murguia-Favela, Luis, Parikh, Suhag, Touzot, Fabien, Cowan, Morton, Dvorak, Christopher, Haddad, Elie, Kohn, Donald, Notarangelo, Luigi, Pai, Sung-Yun, Puck, Jennifer, Pulsipher, Michael, Torgerson, Troy, Kang, Elizabeth, Malech, Harry, Segre, Julia, Bryant, Clare, Holland, Steven, and Falcone, Emilia
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CGD ,Chronic granulomatous disease ,IBD ,NADPH oxidase ,dysbiosis ,inborn errors of immunity ,inflammatory bowel disease ,intestinal inflammation ,metabolome ,microbiome ,primary immune deficiency ,Humans ,Granulomatous Disease ,Chronic ,Gastrointestinal Microbiome ,NADPH Oxidases ,Cross-Sectional Studies ,Inflammatory Bowel Diseases - Abstract
BACKGROUND: Chronic granulomatous disease (CGD) is caused by defects in any 1 of the 6 subunits forming the nicotinamide adenine dinucleotide phosphate oxidase complex 2 (NOX2), leading to severely reduced or absent phagocyte-derived reactive oxygen species production. Almost 50% of patients with CGD have inflammatory bowel disease (CGD-IBD). While conventional IBD therapies can treat CGD-IBD, their benefits must be weighed against the risk of infection. Understanding the impact of NOX2 defects on the intestinal microbiota may lead to the identification of novel CGD-IBD treatments. OBJECTIVE: We sought to identify microbiome and metabolome signatures that can distinguish individuals with CGD and CGD-IBD. METHODS: We conducted a cross-sectional observational study of 79 patients with CGD, 8 pathogenic variant carriers, and 19 healthy controls followed at the National Institutes of Health Clinical Center. We profiled the intestinal microbiome (amplicon sequencing) and stool metabolome, and validated our findings in a second cohort of 36 patients with CGD recruited through the Primary Immune Deficiency Treatment Consortium. RESULTS: We identified distinct intestinal microbiome and metabolome profiles in patients with CGD compared to healthy individuals. We observed enrichment for Erysipelatoclostridium spp, Sellimonas spp, and Lachnoclostridium spp in CGD stool samples. Despite differences in bacterial alpha and beta diversity between the 2 cohorts, several taxa correlated significantly between both cohorts. We further demonstrated that patients with CGD-IBD have a distinct microbiome and metabolome profile compared to patients without CGD-IBD. CONCLUSION: Intestinal microbiome and metabolome signatures distinguished patients with CGD and CGD-IBD, and identified potential biomarkers and therapeutic targets.
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- 2023
12. Abelmoschus manihot polysaccharide fortifies intestinal mucus barrier to alleviate intestinal inflammation by modulating Akkermansia muciniphila abundance
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Yumeng Wang, Chengxi Li, Jianping Li, Shu Zhang, Qinyu Zhang, Jinao Duan, and Jianming Guo
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Plant polysaccharide ,Abelmoschus manihot ,Intestinal inflammation ,Mucus barrier ,Interleukin 10 ,Gut microbiota ,Therapeutics. Pharmacology ,RM1-950 - Abstract
The intestinal mucus barrier is an important line of defense against gut pathogens. Damage to this barrier brings bacteria into close contact with the epithelium, leading to intestinal inflammation. Therefore, its restoration is a promising strategy for alleviating intestinal inflammation. This study showed that Abelmoschus manihot polysaccharide (AMP) fortifies the intestinal mucus barrier by increasing mucus production, which plays a crucial role in the AMP-mediated amelioration of colitis. IL-10-deficient mouse models demonstrated that the effect of AMP on mucus production is dependent on IL-10. Moreover, bacterial depletion and replenishment confirmed that the effects of AMP on IL-10 secretion and mucus production were mediated by Akkermansia muciniphila. These findings suggest that plant polysaccharides fortify the intestinal mucus barrier by maintaining homeostasis in the gut microbiota. This demonstrates that targeting mucus barrier is a promising strategy for treating intestinal inflammation.
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- 2024
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13. Progress of Research on Mechanism of Intestinal Nervous System Regulating Intestinal Inflammation Based on Intestinal Stem Cells
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CHEN Siqi, XIAO Jin, TIAN Siyu, ZHANG Jia, WANG Shuting, ZHANG Xindan, ZHU Yan, CHEN Min
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intestinal inflammation ,inflammatory bowel diseases ,stem cell ,enteric nervous system ,therapy ,review ,Medicine - Abstract
Intestinal stem cells are regulated by the intestinal nervous system, and both of them are closely related to intestinal inflammation. Relevant studies have shown the existence of intestinal nerve dysfunction in intestinal inflammation. By summarizing the relationship among intestinal stem cells, intestinal nervous system and intestinal inflammation, this paper discusses that intestinal nervous system injury related to intestinal inflammation may be the basis for persistent alteration of intestinal function, while enteric neurons provide feasible targets for improving intestinal nerve dysfunction in inflammatory bowel diseases, so as to apply various types of stem cells to the improvement of intestinal nerve dysfunction in intestinal inflammation.
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- 2024
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14. Discovering the Nutrition-Microbiota Interplay in Inflammatory Bowel Disease: Are We There Yet?
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Florio, Marilina, Crudele, Lucilla, Moschetta, Antonio, Gadaleta, Raffaella M., Lenzi, Andrea, Series Editor, Jannini, Emmanuele A., Series Editor, Federici, Massimo, editor, and Menghini, Rossella, editor
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- 2024
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15. the Prevalence of Chronic Rhinosinusitis Associated With Inflammatory Bowel Disease and Association With Biomarkers of Epithelial Barrier Damage (PRIME)
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- 2023
16. Dysregulated brain-gut axis in the setting of traumatic brain injury: review of mechanisms and anti-inflammatory pharmacotherapies
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Mahmoud G. El Baassiri, Zachariah Raouf, Sarah Badin, Alejandro Escobosa, Chhinder P. Sodhi, and Isam W. Nasr
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TBI ,Brain-gut axis ,Intestinal inflammation ,Microbiome ,Enteroendocrine cell ,Neurology. Diseases of the nervous system ,RC346-429 - Abstract
Abstract Traumatic brain injury (TBI) is a chronic and debilitating disease, associated with a high risk of psychiatric and neurodegenerative diseases. Despite significant advancements in improving outcomes, the lack of effective treatments underscore the urgent need for innovative therapeutic strategies. The brain-gut axis has emerged as a crucial bidirectional pathway connecting the brain and the gastrointestinal (GI) system through an intricate network of neuronal, hormonal, and immunological pathways. Four main pathways are primarily implicated in this crosstalk, including the systemic immune system, autonomic and enteric nervous systems, neuroendocrine system, and microbiome. TBI induces profound changes in the gut, initiating an unrestrained vicious cycle that exacerbates brain injury through the brain-gut axis. Alterations in the gut include mucosal damage associated with the malabsorption of nutrients/electrolytes, disintegration of the intestinal barrier, increased infiltration of systemic immune cells, dysmotility, dysbiosis, enteroendocrine cell (EEC) dysfunction and disruption in the enteric nervous system (ENS) and autonomic nervous system (ANS). Collectively, these changes further contribute to brain neuroinflammation and neurodegeneration via the gut-brain axis. In this review article, we elucidate the roles of various anti-inflammatory pharmacotherapies capable of attenuating the dysregulated inflammatory response along the brain-gut axis in TBI. These agents include hormones such as serotonin, ghrelin, and progesterone, ANS regulators such as beta-blockers, lipid-lowering drugs like statins, and intestinal flora modulators such as probiotics and antibiotics. They attenuate neuroinflammation by targeting distinct inflammatory pathways in both the brain and the gut post-TBI. These therapeutic agents exhibit promising potential in mitigating inflammation along the brain-gut axis and enhancing neurocognitive outcomes for TBI patients.
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- 2024
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17. 抗菌肽CC34 对小鼠肠炎的缓解作用.
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董立强, 李云鹤, and 张春华
- Abstract
To reveal the alleviating effect and mechanism of antimicrobial peptide CC34 on lipopolysaccharide (LPS) - induced intestinal inflammation in mice, the experiment selected 40 SPF mice, randomly divided into four groups, with ten mice in each group. Mice in the CC34 group and the LPS+CC34 group were intraperitoneally injected with 9 mg/kg CC34, while mice in the LPS group and Control group were injected with an equal volume of saline, for a duration of seven days. The mice in the LPS group and LPS+CC34 group were injected with 7.5 mg/kg LPS, the CC34 group and Control group injected with equal volume normal saline. The results showed that compared to the Control group, there were no significant changes in the liver, spleen, and heart indices of mice in the CC34 group (P>0.05), but the colon length extremely increased (P<0.01). Compared to the LPS group, the bacterial load in the liver and the content of inflammatory factors in the duodenum and ileum of mice in the LPS+CC34 group extremely decreased (P<0.01), and the gene expression levels of ZO-1, Claudin-1, and Occludin in the intestinal tissue extremely increased (P<0.01), and the villus height/crypt depth ratio (V/C) in the duodenum significantly increased (P<0.05). The gene expression level of myeloperoxidase (MPO) in the intestinal tissue extremely decreased (P<0.01). The gene expression and activity of superoxide dismutase (SOD) and catalase (CAT) in the intestinal tissue extremely increased (P<0.01), the content of malondialdehyde (MDA) extremely decreased (P<0.01), and the total antioxidant capacity (T-AOC) extremely enhanced (P<0.01). The gene expression levels of toll-like receptor-4 (TLR-4) and myeloid differentiation factor 88 (MyD88) in the intestinal tissue extremely down-regulated (P<0.01), and the phosphorylation level of p65 in the duodenum extremely decreased (P<0.01). The study indicates that CC34 alleviates intestinal inflammation in mice by down-regulating the TLR-4/NF-κB signaling pathway. [ABSTRACT FROM AUTHOR]
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- 2024
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18. Gut Microbiota Alleviates Intestinal Injury Induced by Extended Exposure to Light via Inhibiting the Activation of NLRP3 Inflammasome in Broiler Chickens.
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Ma, Dandan, Zhang, Minhong, and Feng, Jinghai
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GUT microbiome , *NLRP3 protein , *BROILER chickens , *INTESTINAL injuries , *INFLAMMASOMES - Abstract
Light pollution is a potential risk for intestinal health in humans and animals. The gut microbiota is associated with the development of intestinal inflammation induced by extended exposure to light, but the underlying mechanism is not yet clear. The results of this study showed that extended exposure to light (18L:6D) damaged intestinal morphology, downregulated the expression of tight junction proteins, and upregulated the expression of the NLRP3 inflammasome and the concentration of pro-inflammatory cytokines. In addition, extended exposure to light significantly decreased the abundance of Lactobacillus, Butyricicoccus, and Sellimonas and increased the abundance of Bifidobacterium, unclassified Oscillospirales, Family_XIII_UCG-001, norank_f__norank_o__Clostridia_vadinBB60_group, and Defluviitaleaceae_UCG-01. Spearman correlation analysis indicated that gut microbiota dysbiosis positively correlated with the activation of the NLRP3 inflammasome. The above results indicated that extended exposure to light induced intestinal injury by NLRP3 inflammasome activation and gut microbiota dysbiosis. Antibiotic depletion intestinal microbiota treatment and cecal microbiota transplantation (CMT) from the 12L:12D group to 18L:6D group indicated that the gut microbiota alleviated intestinal inflammatory injury induced by extended exposure to light via inhibiting the activation of the NLRP3 inflammasome. In conclusion, our findings indicated that the gut microbiota can alleviate intestinal inflammation induced by extended exposure to light via inhibiting the activation of the NLRP3 inflammasome. [ABSTRACT FROM AUTHOR]
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- 2024
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19. Genistein Promotes M2 Macrophage Polarization via Aryl Hydrocarbon Receptor and Alleviates Intestinal Inflammation in Broilers with Necrotic Enteritis.
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Quan, Shuli, Huang, Jingxi, Chen, Guiqin, Zhang, Anrong, Yang, Ying, and Wu, Zhenlong
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ARYL hydrocarbon receptors , *NECROTIC enteritis , *TRANSCRIPTION factors , *GENISTEIN , *INTESTINES , *MACROPHAGES - Abstract
The aryl hydrocarbon receptor (AhR) is a transcription factor that regulates the immune system through complicated transcriptional programs. Genistein, an AhR ligand, exhibits anti-inflammatory properties. However, its role in modulating immune responses via the AhR signaling pathway remains unclear. In this study, 360 male Arbor Acre broilers (1-day-old) were fed a basal diet supplemented with 40 or 80 mg/kg genistein and infected with or without Clostridium perfringens (Cp). Our results demonstrated that genistein ameliorated Cp-induced intestinal damage, as reflected by the reduced intestinal lesion scores and improved intestinal morphology and feed-to-gain ratio. Moreover, genistein increased intestinal sIgA, TGF-β, and IL-10, along with elevated serum IgG, IgA, and lysozyme levels. Genistein improved intestinal AhR and cytochrome P450 family 1 subfamily A member 1 (CYP1A1) protein levels and AhR+ cell numbers in Cp-challenged broilers. The increased number of AhR+CD163+ cells in the jejunum suggested a potential association between genistein-induced AhR activation and anti-inflammatory effects mediated through M2 macrophage polarization. In IL-4-treated RAW264.7 cells, genistein increased the levels of AhR, CYP1A1, CD163, and arginase (Arg)-1 proteins, as well as IL-10 mRNA levels. This increase was attenuated by the AhR antagonist CH223191. In summary, genistein activated the AhR signaling pathway in M2 macrophages, which enhanced the secretion of anti-inflammatory cytokines and attenuated intestinal damage in Cp-infected broilers Cp. [ABSTRACT FROM AUTHOR]
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- 2024
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20. Intestinal Epithelial Creatine Transporter SLC6A8 Dysregulation in Inflammation and in Response to Adherent Invasive E. coli Infection.
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Sawant, Harshal, Selvaraj, Rajesh, Manogaran, Prasath, and Borthakur, Alip
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ESCHERICHIA coli , *INFLAMMATORY bowel diseases , *CREATINE , *INTESTINES , *INFLAMMATION - Abstract
Creatine transporter (CrT1) mediates cellular uptake of creatine (Cr), a nutrient pivotal in maintaining energy homeostasis in various tissues including intestinal epithelial cells (IECs). The impact of CrT1 deficiency on the pathogenesis of various psychiatric and neurological disorders has been extensively investigated. However, there are no studies on its regulation in IECs in health and disease. Current studies have determined differential expression of CrT1 along the length of the mammalian intestine and its dysregulation in inflammatory bowel disease (IBD)-associated inflammation and Adherent Invasive E. coli (AIEC) infection. CrT1 mRNA and protein levels in normal intestines and their alterations in inflammation and following AIEC infection were determined in vitro in model IECs (Caco-2/IEC-6) and in vivo in SAMP1/YitFc mice, a model of spontaneous ileitis resembling human IBD. CrT1 is differentially expressed in different regions of mammalian intestines with its highest expression in jejunum. In vitro, CrT1 function (Na+-dependent 14C-Cr uptake), expression and promoter activity significantly decreased following TNFα/IL1β treatments and AIEC infection. SAMP1 mice and ileal organoids generated from SAMP1 mice also showed decreased CrT1 mRNA and protein compared to AKR controls. Our studies suggest that Cr deficiency in IECs secondary to CrT1 dysregulation could be a key factor contributing to IBD pathogenesis. [ABSTRACT FROM AUTHOR]
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- 2024
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21. <italic>Inonotus obliquus</italic> aqueous extract inhibits intestinal inflammation and insulin metabolism defects in <italic>Drosophila</italic>.
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Yu, Shichao, Lai, Zhixian, Xue, Hongmei, Zhu, Jiahua, Yue, Guanhua, Wang, Jiewei, and Jin, LiHua
- Abstract
AbstractIn biomedical research, the fruit fly (
Drosophila melanogaster ) is among the most effective and flexible model organisms. Through the use of theDrosophila model, molecular mechanisms of human diseases can be investigated and candidate pharmaceuticals can be screened. White rot fungusInonotus obliquus is a member of the familyHymenochaetaceae . Due to its multifaceted pharmacological effects, this fungus has been the subject of scientific investigation. Nevertheless, the precise mechanisms by whichInonotus obliquus treats diseases remain unclear. In this study, we prepared an aqueous extract derived fromInonotus obliquus and demonstrated that it effectively prevented the negative impacts of inflammatory agents on flies, including overproliferation and overdifferentiation of intestinal progenitor cells and decreased survival rate. Furthermore, elevated reactive oxygen species levels and cell death were alleviated byInonotus obliquus aqueous extract, suggesting that this extract inhibited intestinal inflammation. Additionally,Inonotus obliquus aqueous extract had an impact on the insulin pathway, as it alleviated growth defects in flies that were fed a high-sugar diet and inchico mutants. In addition, we determined the composition ofInonotus obliquus aqueous extract and conducted a network pharmacology analysis in order to identify prospective key compounds and targets. In brief,Inonotus obliquus aqueous extract exhibited considerable potential as a therapeutic intervention for human diseases. Our research has established a foundational framework that supports the potential clinical implementation ofInonotus obliquus . [ABSTRACT FROM AUTHOR]- Published
- 2024
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22. Milk and Lacticaseibacillus paracasei BL23 effects on intestinal responses in a murine model of colitis.
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Bui, Glory, Torres-Fuentes, Cristina, Pusceddu, Matteo M., Gareau, Mélanie G., and Marco, Maria L.
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GOAT milk , *COLITIS , *WEIGHT loss , *GUT microbiome , *FERMENTED foods , *ION transport (Biology) - Abstract
Probiotic-containing fermented dairy foods have the potential to benefit human health, but the importance of the dairy matrix for efficacy remains unclear. We investigated the capacity of Lacticaseibacillus paracasei BL23 in phosphate-buffered saline (BL23-PBS), BL23-fermented milk (BL23-milk), and milk to modify intestinal and behavioral responses in a dextran sodium sulfate (DSS, 3% wt/vol) mouse model of colitis. Significant sex-dependent differences were found such that female mice exhibited more severe colitis, greater weight loss, and higher mortality rates. Sex differences were also found for ion transport ex vivo, colonic cytokine and tight junction gene expression, and fecal microbiota composition. Measurements of milk and BL23 effects showed BL23-PBS consumption improved weight recovery in females, whereas milk resulted in better body weight recovery in males. Occludin and Claudin-2 gene transcript levels indicated barrier function was impaired in males, but BL23-milk was still found to improve colonic ion transport in those mice. Proinflammatory and anti-inflammatory gene expression levels were increased in both male and female mice fed BL23, and to a more variable extent, milk, compared with controls. The female mouse fecal microbiota contained high proportions of Akkermansia (average of 18.1%) at baseline, and females exhibited more changes in gut microbiota composition following BL23 and milk intake. Male fecal microbiota harbored significantly more Parasutterella and less Blautia and Roseburia after DSS treatment, independent of BL23 or milk consumption. These findings show the complex interplay between dietary components and sex-dependent responses in mitigating inflammation in the digestive tract. NEW & NOTEWORTHY: Sex-dependent responses to probiotic Lacticaseibacillus paracasei and milk and the potential of the dairy matrix to enhance probiotic protection against colitis in this context have not been previously explored. Female mice were more sensitive than males to colonic injury, and neither treatment effectively alleviated inflammation in both sexes. These sex-dependent responses may result from differences in the higher baseline proportions of Akkermansia in the gut microbiome of female mice. Listen to this article's corresponding podcast at https://ajpgi.podbean.com/e/got-guts-the-micro-version-effects-of-l-casei-fermented-milk-on-mice-with-dss-colitis/. [ABSTRACT FROM AUTHOR]
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- 2024
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23. Cynaroside ameliorates methotrexate-induced enteritis in rats through inhibiting NLRP3 inflammasome activation.
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Wuying Lang, Xin Wen, Shuangqi Zhang, Xuhua Liang, Lin Chen, Dezhu Zhang, Ruina Zhou, Ihsan Ali, Xuansheng Hu, Haihua Zhang, and Min Cheng
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WEIGHT loss ,NLRP3 protein ,INFLAMMASOMES ,ENTERITIS ,RATS ,INTESTINAL ischemia - Abstract
Introduction: Cynaroside exhibits various biological properties, including anti-inflammatory, antiviral, antitumor, and cardioprotective effects. However, its involvement in methotrexate (MTX)-induced intestinal inflammation remains inadequately understood. Thus, we investigated the impact of cynaroside on MTX-induced intestinal inflammation and its potential mechanisms. Methods: To assess the protective potential of cynaroside against intestinal inflammation, Sprague-Dawley rats were subjected to a regimen of 7 mg/kg MTX for 3 days, followed by treatment with cynaroside at varying doses (10, 20, or 40 mg/kg). Histopathological evaluations were conducted alongside measurements of inflammatory mediators to elucidate the involvement of the NLRP3 inflammasome in alleviating intestinal inflammation. Results: Administration of 7 mg/kg MTX resulted in decreased daily food intake, increased weight loss, and elevated disease activity index in rats. Conversely, treatment with cynaroside at 20 or 40 mg/kg ameliorated the reductions in body weight and daily food intake and suppressed the MTX-induced elevation in the disease activity index. Notably, cynaroside administration at 20 or 40 mg/kg attenuated inflammatory cell infiltration, augmented goblet cell numbers and lowered serum levels of tumor necrosis factor-α, interleukin (IL)-1β, and IL-18, as well as the CD68-positive cell rate in the intestines of MTX-induced rats. Furthermore, cynaroside downregulated the expression levels of NLRP3, cleaved caspase 1, and cleaved IL-1β in MTX-induced rats. Discussion: Collectively, our findings indicated that cymaroside alleviates intestinal inflammatory injury by inhibiting the activation of NLRP3 inflammasome in MTX-induced rats. [ABSTRACT FROM AUTHOR]
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- 2024
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24. Endoplasmic reticulum stress: A possible connection between intestinal inflammation and neurodegenerative disorders.
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Vivacqua, Giorgio, Mancinelli, Romina, Leone, Stefano, Vaccaro, Rosa, Garro, Ludovica, Carotti, Simone, Ceci, Ludovica, Onori, Paolo, Pannarale, Luigi, Franchitto, Antonio, Gaudio, Eugenio, and Casini, Arianna
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ENDOPLASMIC reticulum , *INFLAMMATORY bowel diseases , *NEURODEGENERATION , *INTESTINES , *INFLAMMATION - Abstract
Background: Different studies have shown the key role of endoplasmic reticulum (ER) stress in autoimmune and chronic inflammatory disorders, as well as in neurodegenerative diseases. ER stress leads to the formation of misfolded proteins which affect the secretion of different cell types that are crucial for the intestinal homeostasis. Purpose: In this review, we discuss the role of ER stress and its involvement in the development of inflammatory bowel diseases, chronic conditions that can cause severe damage of the gastrointestinal tract, focusing on the alteration of Paneth cells and goblet cells (the principal secretory phenotypes of the intestinal epithelial cells). ER stress is also discussed in the context of neurodegenerative diseases, in which protein misfolding represents the signature mechanism. ER stress in the bowel and consequent accumulation of misfolded proteins might represent a bridge between bowel inflammation and neurodegeneration along the gut‐to‐brain axis, affecting intestinal epithelial homeostasis and the equilibrium of the commensal microbiota. Targeting intestinal ER stress could foster future studies for designing new biomarkers and new therapeutic approaches for neurodegenerative disorders. [ABSTRACT FROM AUTHOR]
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- 2024
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25. Supercritical fluid carbon dioxide extract of Alpinia oxyphylla ameliorates dextran sulfate sodium-induced intestinal barrier damage in mice.
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Fan Yang, Yongqing Tao, Chen Chen, Wei Zhao, Juan Wang, Beibei Peng, Ke Lv, Erah, Patrick O., and Hui Zhao
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- *
DEXTRAN sulfate , *SUPERCRITICAL carbon dioxide , *PLANT extracts , *ALPINIA , *INTESTINAL barrier function , *INTESTINES , *OILSEEDS , *FLUORESCEIN isothiocyanate - Abstract
Purpose: Inflammation and oxidative stress are the leading causes of intestinal barrier dysfunction and a wide range of diseases. The purpose of this study was to explore the protective effect and elucidate potential mechanisms of supercritical carbon dioxide extract from Alpinia oxyphylla Miq. (AOE) on intestinal inflammation. Methods: The AOE was extracted by supercritical carbon dioxide extraction and its components were determined. Cytotoxicity of the extract (0.05 to 20µg/mL) was determined on Caco-2 cells. In vitro assessment of whether AOE protected against LPS-induced intestinal barrier dysfunction was done on Caco-2 cells pretreated with non-cytotoxic concentrations (0.5µg/mL and 1 µg/mL) of the extract. Experimental colitis of mouse model was induced by drinking water containing 3% dextran sulfate sodium (DSS). The intestinal permeability was assessed by TEER and fluorescein isothiocyanate conjugated dextran (FITC). Pathological evidence and possible mechanism were verified by tissue staining and molecular methods including quantitative real-time polymerase chain reaction (qRT-PCR) and western blot. Results: TEER and FITC testing indicated that AOE pretreatment significantly improved intestinal barrier hemostasis (p<0.05). Furthermore, AOE pretreatment counteracted DSS-induced upregulation of pro-inflammatory cytokines and oxidative stress (p<0.05). Lastly, two crucial signal pathways regarding hemostasis of intestinal barrier, NF-κB and NOX1-LCN2, were significantly attenuated upon AOE pretreatment (p<0.05). Conclusion: This study sheds lights on the protective effect of AOE for intestinal hemostasis and supports the development of AOE-based intestinal protective agents. [ABSTRACT FROM AUTHOR]
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- 2024
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26. Epithelial-neuronal-immune cell interactions: Implications for immunity, inflammation, and tissue homeostasis at mucosal sites.
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Emanuel, Elizabeth, Arifuzzaman, Mohammad, and Artis, David
- Abstract
The epithelial lining of the respiratory tract and intestine provides a critical physical barrier to protect host tissues against environmental insults, including dietary antigens, allergens, chemicals, and microorganisms. In addition, specialized epithelial cells communicate directly with hematopoietic and neuronal cells. These epithelial-immune and epithelial-neuronal interactions control host immune responses and have important implications for inflammatory conditions associated with defects in the epithelial barrier, including asthma, allergy, and inflammatory bowel diseases. In this review, we discuss emerging research that identifies the mechanisms and impact of epithelial-immune and epithelial-neuronal cross talk in regulating immunity, inflammation, and tissue homeostasis at mucosal barrier surfaces. Understanding the regulation and impact of these pathways could provide new therapeutic targets for inflammatory diseases at mucosal sites. [ABSTRACT FROM AUTHOR]
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- 2024
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27. Pharmacological effects of ginseng and ginsenosides on intestinal inflammation and the immune system.
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Linxian Zhao, Tongbo Zhang, and Kai Zhang
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GINSENOSIDES ,INFLAMMATORY bowel diseases ,IRRITABLE colon ,GINSENG ,INTESTINES ,IMMUNE system - Abstract
Intestinal inflammatory imbalance and immune dysfunction may lead to a spectrum of intestinal diseases, such as inflammatory bowel disease (IBD) and gastrointestinal tumors. As the king of herbs, ginseng has exerted a wide range of pharmacological effects in various diseases. Especially, it has been shown that ginseng and ginsenosides have strong immunomodulatory and antiinflammatory abilities in intestinal system. In this review, we summarized how ginseng and various extracts influence intestinal inflammation and immune function, including regulating the immune balance, modulating the expression of inflammatory mediators and cytokines, promoting intestinal mucosal wound healing, preventing colitis-associated colorectal cancer, recovering gut microbiota and metabolism imbalance, alleviating antibiotic-induced diarrhea, and relieving the symptoms of irritable bowel syndrome. In addition, the specific experimental methods and key control mechanisms are also briefly described. [ABSTRACT FROM AUTHOR]
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- 2024
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28. Ketogenic Diet Protects from Experimental Colitis in a Mouse Model Regardless of Dietary Fat Source.
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Luiskari, Lotta, Lindén, Jere, Lehto, Markku, Salmenkari, Hanne, and Korpela, Riitta
- Abstract
While ketogenic diets (KDs) may have potential as adjunct treatments for gastrointestinal diseases, there is little knowledge on how the fat source of these diets impacts intestinal health. The objective of this study was to investigate how the source of dietary fat of KD influences experimental colitis. We fed nine-week-old male C57BL/6J mice (n = 36) with a low-fat control diet or KD high either in saturated fatty acids (SFA-KD) or polyunsaturated linoleic acid (LA-KD) for four weeks and then induced colitis with dextran sodium sulfate (DSS). To compare the diets, we analyzed macroscopic and histological changes in the colon, intestinal permeability to fluorescein isothiocyanate−dextran (FITC–dextran), and the colonic expression of tight junction proteins and inflammatory markers. While the effects were more pronounced with LA-KD, both KDs markedly alleviated DSS-induced histological lesions. LA-KD prevented inflammation-related weight loss and the shortening of the colon, as well as preserved Il1b and Tnf expression at a healthy level. Despite no significant between-group differences in permeability to FITC–dextran, LA-KD mitigated changes in tight junction protein expression. Thus, KDs may have preventive potential against intestinal inflammation, with the level of the effect being dependent on the dietary fat source. [ABSTRACT FROM AUTHOR]
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- 2024
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29. Early and aggressive treatment for Crohn disease using biologics and immunomodulators.
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Martin, Erin and Sadowski, Catherine K.
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BIOTHERAPY ,PHYSICAL diagnosis ,RISK assessment ,CROHN'S disease ,EARLY medical intervention ,COMPUTED tomography ,SEVERITY of illness index ,ENDOSCOPIC surgery ,INFLAMMATORY bowel diseases ,IMMUNOMODULATORS ,ENDOSCOPY ,DISEASE progression ,SYMPTOMS - Abstract
Traditionally, medical providers have used the step-up approach to manage patients with Crohn disease, starting with 5-aminosalicylic acid derivatives, progressing to corticosteroids, and eventually to immunomodulators and biologics. However, a new top-down approach focuses on early and aggressive therapy with biologics and immunomodulators to reduce the rate of mucosal and intestinal damage. This article describes early and aggressive biologic and immunomodulator therapies and new therapeutic parameters compared with traditional step-up treatment for patients with Crohn disease. [ABSTRACT FROM AUTHOR]
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- 2024
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30. Arginine alleviates Clostridium perfringens α toxin-induced intestinal injury in vivo and in vitro via the SLC38A9/mTORC1 pathway.
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Xiaohui Wang, Tong Zhang, Wenli Li, Heliang Wang, Lei Yan, Xiaowen Zhang, Lianwen Zhao, Nianxue Wang, and Beibei Zhang
- Subjects
CLOSTRIDIUM perfringens ,ARGININE ,INTESTINAL injuries ,RIBOSOMAL proteins ,GENE expression ,CYTOTOXINS - Abstract
Introduction: Clostridium perfringens α toxin is a main virulence factor responsible for gut damage in animals. Arginine is a functional amino acid exhibiting significant immunoregulatory activities. However, the effects and immunoregulatory mechanisms of arginine supplementation on α toxin-induced intestinal injury remain unclear. Methods: In vivo, 256 male Arbor Acres chickens were randomly assigned to a 2x2 factorial arrangement, involving diet treatments (with or without 0.3% arginine supplementation) and immunological stress (with or without α toxin challenge). In vitro, IEC-6 cells were treated with or without arginine in the presence or absence of α toxin. Moreover, IEC-6 cells were transfected with siRNA targeting mTOR and SLC38A9 to explore the underlying mechanisms. Results and discussion: The results showed that in vivo, arginine supplementation significantly alleviated the α toxin-induced growth performance impairment, decreases in serum immunoglobulin (Ig)A and IgG levels, and intestinal morphology damage. Arginine supplementation also significantly reduced the α toxin-induced increase in jejunal proinflammatory cytokines interleukin (IL)-1β, IL-6 and IL-17 mRNA expression. Clostridium perfringens α toxin significantly decreased jejunal mechanistic target of rapamycin (mTOR) and solute carrier family 38 member 9 (SLC38A9) mRNA expression, while arginine supplementation significantly increased mTOR and SLC38A9 mRNA expression. In vitro, arginine pretreatment mitigated the α toxin-induced decrease in cell viability and the increase in cytotoxicity and apoptosis. Arginine pretreatment also alleviated the α toxin-induced upregulation of mRNA expression of inflammation-related cytokines IL-6, C-X-C motif chemokine ligand (CXCL)10, CXCL11 and transforming growth factor-β (TGF-β), as well as apoptosis-related genes B-cell lymphoma-2 associated X protein (Bax), B-cell lymphoma-2 (Bcl-2), B-cell lymphoma-extra large (Bcl-XL) and cysteinyl aspartate specific proteinase 3 (Caspase-3) and the ratio of Bax to Bcl-2. Arginine pretreatment significantly increased the α toxin-induced decrease in mTOR, SLC38A9, eukaryotic translation initiation factor 4E (eIF4E)-binding protein 1 (4EBP1) and ribosomal protein S6 kinase (S6K) mRNA expression. Knockdown SLC38A9 and mTOR largely abrogated the positive effects of arginine pretreatment on α toxin-induced intracellular changes. Furthermore, SLC38A9 silencing abolished the increased mTOR mRNA expression caused by arginine pretreatment. In conclusion, arginine administration attenuated α toxin-induced intestinal injury in vivo and in vitro, which could be associated with the downregulation of inflammation via regulating SLC38A9/mTORC1 pathway. [ABSTRACT FROM AUTHOR]
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- 2024
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31. m6A Methylated Long Noncoding RNA LOC339803 Regulates Intestinal Inflammatory Response.
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Olazagoitia‐Garmendia, Ane, Rojas‐Márquez, Henar, Sebastian‐delaCruz, Maialen, Agirre‐Lizaso, Aloña, Ochoa, Anne, Mendoza‐Gomez, Luis Manuel, Perugorria, Maria J, Bujanda, Luis, Madrigal, Alain Huerta, Santin, Izortze, and Castellanos‐Rubio, Ainara
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LINCRNA , *INFLAMMATION , *RNA modification & restriction , *INTESTINES , *INFLAMMATORY mediators , *DISEASE susceptibility - Abstract
Cytokine mediated sustained inflammation increases the risk to develop different complex chronic inflammatory diseases, but the implicated mechanisms remain unclear. Increasing evidence shows that long noncoding RNAs (lncRNAs) play key roles in the pathogenesis of inflammatory disorders, while inflammation associated variants are described to affect their function or essential RNA modifications as N6‐methyladenosine (m6A) methylation, increasing predisposition to inflammatory diseases. Here, the functional implication of the intestinal inflammation associated lncRNA LOC339803 in the production of cytokines by intestinal epithelial cells is described. Allele‐specific m6A methylation is found to affect YTHDC1 mediated protein binding affinity. LOC339803‐YTHDC1 interaction dictates chromatin localization of LOC339803 ultimately inducing the expression of NFκB mediated proinflammatory cytokines and contributing to the development of intestinal inflammation. These findings are confirmed using human intestinal biopsy samples from different intestinal inflammatory conditions and controls. Additionally, it is demonstrated that LOC339803 targeting can be a useful strategy for the amelioration of intestinal inflammation in vitro and ex vivo. Overall, the results support the importance of the methylated LOC339803 lncRNA as a mediator of intestinal inflammation, explaining genetic susceptibility and presenting this lncRNA as a potential novel therapeutic target for the treatment of inflammatory intestinal disorders. [ABSTRACT FROM AUTHOR]
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- 2024
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32. Vitamin C Alleviates Intestinal Inflammation Caused by Aeromonas hydrophila in Juvenile Blunt Snout Bream (Megalobrama amblycephala).
- Author
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Muhammad, Abdullateef Mukhtar, Yang, Chang, Wang, Jingyuan, Ge, Xianping, Liu, Bo, Miao, Linghong, Gao, Guodong, and Zhou, Qunlan
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- *
VITAMIN C , *SEBASTES marinus , *NATURAL immunity , *FEED utilization efficiency , *INFLAMMATION , *IMMUNE serums , *AEROMONAS hydrophila - Abstract
Vitamin C (VC) can be used to increase disease resistance in practice in intensive aquaculture. But it is still unconfirmed whether VC could alleviate inflammation and what dosage is suitable. This study investigated the effects of dietary VC on the immunity and enteritis of juvenile blunt snout bream (Megalobrama amblycephala) challenged with Aeromonas hydrophila. The fish were fed with VC levels ranging from 25.35 to 2231.98 mg/kg for 60 days. After that, fish fed with 150.65 mg/kg, 573.79 mg/kg, and 2231.98 mg/kg VC were challenged with A. hydrophila orally to simulate enteritis. The results showed that 285.39 and 573.79 mg/kg dietary VC significant improved growth performance and feed utilization. Dietary VC (573.79 and 1133.79 mg/kg) significantly enhanced the serum immune parameters, antioxidant enzymes' activities, and relative gene expressions of ikbα and cat in the liver in the 60-day feeding trail. The survival rate was significantly higher in 573.79 mg/kg dietary VC group than the other two treatments. Cytokines were activated after the bacteria challenge. Dietary 573.79 mg/kg VC caused significantly higher TNF-α level at 12 hpi and maintained a high level of IL-8 at 48 to 72 h compared to other treatments. In conclusion, more than 500 mg/kg VC was needed to maintain the health of blunt snout bream juveniles. Suitable VC would activate cytokines to increase disease resistance along with antioxidant enzymes. [ABSTRACT FROM AUTHOR]
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- 2024
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33. Whole genome sequencing analysis of Limosilactobacillus reuteri from the intestinal tract of mice recovering from ulcerative colitis and preliminary study on anti-inflammatory effects of its derived peptides.
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Wang, Ziyan, Zhang, Zhixuan, Shi, Qiuyue, Liu, Songyi, Wu, Qiaoli, Wang, Ze, saiding, Emilaguli, Han, Jiaojiao, Zhou, Jun, Wang, Rixin, and Su, Xiurong
- Abstract
Limosilactobacillus reuteri is an indigenous inhabitant of the animal gut known for its probiotic effects on the host. In our previous study, a large number of L. reuteri strains were isolated from the gastrointestinal tract of mice recovering from ulcerative colitis, from which we randomly selected L. reuteri RE225 for whole genome sequencing to explore its probiotic properties. The results of next-generation sequencing and third-generation single molecule sequencing showed that L. reuteri RE225 contained many genes encoding functional proteins associated with adhesion, anti-inflammatory and pathogen inhibition. And compared to other L. reuteri strains in NCBI, L. reuteri RE225 has unique gene families with probiotic functions. In order to further explore the probiotic effect of the L. reuteri RE225, the derived peptides were identified by LC-MS/MS, and the peptides with tumor necrosis factor-α binding ability were screened by reverse molecular docking and microscale thermophoresis. Finally, cell experiments demonstrated the anti-inflammatory ability of the peptides. Western blotting and qPCR analyses confirmed that the selected peptides might alleviate LPS-induced inflammation in NCM460 cells by inhibiting JAK2/STAT3 pathway activation. [ABSTRACT FROM AUTHOR]
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- 2024
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34. Intestinal Fibrogenesis in Inflammatory Bowel Diseases: Exploring the Potential Role of Gut Microbiota Metabolites as Modulators.
- Author
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Cicchinelli, Sara, Gemma, Stefania, Pignataro, Giulia, Piccioni, Andrea, Ojetti, Veronica, Gasbarrini, Antonio, Franceschi, Francesco, and Candelli, Marcello
- Subjects
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INFLAMMATORY bowel diseases , *GUT microbiome , *INTESTINES , *PATHOLOGICAL physiology , *EPITHELIAL-mesenchymal transition - Abstract
Fibrosis, sustained by the transformation of intestinal epithelial cells into fibroblasts (epithelial-to-mesenchymal transition, EMT), has been extensively studied in recent decades, with the molecular basis well-documented in various diseases, including inflammatory bowel diseases (IBDs). However, the factors influencing these pathways remain unclear. In recent years, the role of the gut microbiota in health and disease has garnered significant attention. Evidence suggests that an imbalanced or dysregulated microbiota, along with environmental and genetic factors, may contribute to the development of IBDs. Notably, microbes produce various metabolites that interact with host receptors and associated signaling pathways, influencing physiological and pathological changes. This review aims to present recent evidence highlighting the emerging role of the most studied metabolites as potential modulators of molecular pathways implicated in intestinal fibrosis and EMT in IBDs. These studies provide a deeper understanding of intestinal inflammation and fibrosis, elucidating the molecular basis of the microbiota role in IBDs, paving the way for future treatments. [ABSTRACT FROM AUTHOR]
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- 2024
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35. Effect of dietary aspirin eugenol ester on the growth performance, antioxidant capacity, intestinal inflammation, and cecal microbiota of broilers under high stocking density
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Haojie Zhang, Yi Zhang, Dongying Bai, Jiale Zhong, Xiaodi Hu, Ruilin Zhang, Wenrui Zhen, Koichi Ito, Bingkun Zhang, Yajun Yang, Jianyong Li, and Yanbo Ma
- Subjects
broiler ,high stocking density ,aspirin eugenol ester ,antioxidant capacity ,intestinal inflammation ,intestinal microbiota ,Animal culture ,SF1-1100 - Abstract
ABSTRACT: This study was designed to examine the impact of aspirin eugenol ester (AEE) on the growth performance, serum antioxidant capacity, jejunal barrier function, and cecal microbiota of broilers raised under stressful high density (HD) stocking conditions compared with normal density broilers (ND). A total of 432 one-day-old AA+ male broilers were randomly divided into 4 groups: normal density (ND, 14 broilers /m2), high density (HD, 22 broilers /m2), ND + AEE, and HD + AEE. The results of the study revealed a significant decrease in the growth performance of broiler chickens as a result of HD stress (P < 0.05). The total antioxidant capacity (T-AOC) in serum demonstrated a significant decrease (P < 0.05) at both 28 and 35 d. Conversely, the serum level of malondialdehyde (MDA) exhibited a significant increase (P < 0.05). Dietary supplementation of AEE resulted in a significant elevation (P < 0.05) of serum GSH-PX, SOD and T-AOC activity at both 28 and 35 d. Moreover, exposure to HD stress resulted in a considerable reduction in the height of intestinal villi and mRNA expression of tight junction proteins in the jejunum, along with, a significant elevation in the mRNA expression of inflammatory cytokines (P < 0.05). However, the administration of AEE reversed the adverse effects of HD-induced stress on villus height and suppressed the mRNA expression of the pro-inflammatory genes, COX-2 and mPGES-1. Additionally, the exposure to HD stress resulted in a substantial reduction in the α-diversity of cecal microbiota and disruption in the equilibrium of intestinal microbial composition, with a notable decrease in the relative abundance of Bacteroides and Faecalibacterium (P < 0.05). In contrast, the addition of AEE to the feed resulted in a notable increase in the relative abundance of Phascolarctobacterium and enhanced microbial diversity (P < 0.05). The inclusion of AEE in the diet has been demonstrated to enhance intestinal integrity and growth performance of broilers by effectively mitigating disruptions in gut microbiota induced by HD stress.
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- 2024
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36. Insights into the effects of chronic combined chromium-nickel exposure on colon damage in mice through transcriptomic analysis and in vitro gastrointestinal digestion assay
- Author
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Shuangyan Zheng, Zilong Wang, Xianhong Cao, Luqi Wang, Xiaona Gao, Yufan Shen, Jun Du, Ping Liu, Yu Zhuang, and Xiaoquan Guo
- Subjects
Intestinal inflammation ,Goblet cell ,Tight junction ,RNA-seq ,In vitro gastrointestinal digestion ,Environmental pollution ,TD172-193.5 ,Environmental sciences ,GE1-350 - Abstract
Heavy metals interact with each other in a coexisting manner to produce complex combined toxicity to organisms. At present, the toxic effects of chronic co-exposure to heavy metals hexavalent chromium [Cr(VI)] and divalent nickel [Ni(II)] on organisms are seldom studied and the related mechanisms are poorly understood. In this study, we explored the mechanism of the colon injury in mice caused by chronic exposure to Cr or/and Ni. The results showed that, compared with the control group, Cr or/and Ni chronic exposure affected the body weight of mice, and led to infiltration of inflammatory cells in the colon, decreased the number of goblet cells, fusion of intracellular mucus particles and damaged cell structure of intestinal epithelial. In the Cr or/and Ni exposure group, the activity of nitric oxide synthase (iNOS) increased, the expression levels of MUC2 were significantly down-regulated, and those of ZO-1 and Occludin were significantly up-regulated. Interestingly, factorial analysis revealed an interaction between Cr and Ni, which was manifested as antagonistic effects on iNOS activity, ZO-1 and MUC2 mRNA expression levels. Transcriptome sequencing further revealed that the expression of genes-related to inflammation, intestinal mucus and tight junctions changed obviously. Moreover, the relative contents of Cr(VI) and Ni(II) in the Cr, Ni and Cr+Ni groups all changed with in-vitro gastrointestinal (IVG)digestion, especially in the Cr+Ni group. Our results indicated that the chronic exposure to Cr or/and Ni can lead to damage to the mice colon, and the relative content changes of Cr(VI) and Ni(II) might be the main reason for the antagonistic effect of Cr+Ni exposure on the colon damage.
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- 2024
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37. Bowel Sounds Analysis in Paediatric Inflammatory Bowel Disease: Relationship With wPCDAI (SONO-MICI)
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- 2023
38. Effects of multiple pro-inflammatory stimuli in utero on the ileum of extremely premature ovine fetuses.
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Heiter, Julia, Kemp, Matthew W, Spiller, Owen B, Singer, Dominique, Newnham, John P, Kallapur, Suhas G, Jobe, Alan H, and Kramer, Boris W
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Fetus ,Animals ,Sheep ,Sheep ,Domestic ,Humans ,Chorioamnionitis ,Premature Birth ,Inflammation ,Lipopolysaccharides ,Pregnancy ,Infant ,Infant ,Newborn ,Female ,chorioamnionitis ,enteric nervous system ,intestinal inflammation ,necrotizing enterocolitis ,preterm fetal ileum ,Infectious Diseases ,Digestive Diseases ,Preterm ,Low Birth Weight and Health of the Newborn ,Infant Mortality ,Perinatal Period - Conditions Originating in Perinatal Period ,Neurosciences ,Pediatric ,Good Health and Well Being ,Immunology ,Medical Microbiology - Abstract
IntroductionChorioamnionitis is common in preterm birth and associated with a higher risk of intestinal inflammation and necrotizing enterocolitis. The intestinal inflammation influences the enteric nervous system development. We hypothesized that inflammation and innervation in the fetal ileum may be modified by chorioamnionitis induced by repeated challenge with lipopolysaccharide and/or preexisting Ureaplasma parvum infection at very low gestational age equivalent to 60% of term.Materials and methodsTime mated ovine fetuses were exposed by intraamniotic injections to chronic Ureaplasma parvum for 24 days and/or lipopolysaccharide for 7 days, 2 days, or 7 & 2 days before delivery at 94 +/-2 days of gestational age (term at approximately 150 days). Intestinal inflammation as well as structural changes of the enteric nervous system were assessed.ResultsLipopolysaccharide exposure increased CD3 and myeloperoxidase-positive cells (p < 0.05). Repetitive exposure to lipopolysaccharide or combined Ureaplasma parvum & lipopolysaccharide exposure increased intestinal inflammation (p < 0.05). The reduction of nuclei of neurons was most significant with repetitive lipopolysaccharide exposures but could be detected in all other intervention groups compared to the control group. Astrocyte-like glial cells increased if exposure to lipopolysaccharide was only 2 days before delivery or chronic exposure to Ureaplasma parvum existed beforehand (p < 0.05).DiscussionAfter exposure to chorioamnionitis induced by Ureaplasma parvum and/or lipopolysaccharide, inflammatory responses as well as structural changes of the enteric nervous system were more pronounced the longer and the more frequent the exposure to pro-inflammatory stimuli before birth. These changes may cause functional effects of clinical importance.
- Published
- 2023
39. Oral Treatment with Saccharomyces cerevisiae CNCM I-3856 Mitigates the Inflammatory Response Experimentally Induced by Salmonella enterica subsp. enterica Serovar Typhimurium in Mice
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Campos, Lara L., Oliveira, Samantha R. M., Amaral, Maisa N. S., Gallotti, Bruno, Oliveira, Aline F., Arantes, Rosa M. E., Ribeiro-Souza, Samantha, Vital, Katia D., Fernandes, Simone O. A., Cardoso, Valbert N., Nicoli, Jacques R., and Martins, Flaviano S.
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- 2024
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40. A systematic review of preclinical studies targeted toward the management of co-existing functional gastrointestinal disorders, stress, and gut dysbiosis
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Pal, Shreyashi, Arisha, Ruhi, and Mazumder, Papiya Mitra
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- 2024
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41. Relationship between heart failure and intestinal inflammation in infants with congenital heart disease
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Qi-Liang Zhang, Xiu-Hua Chen, Si-Jia Zhou, Yu-Qing Lei, Qiang Chen, and Hua Cao
- Subjects
Congenital heart disease ,Heart failure ,Intestinal microbiota ,Intestinal inflammation ,Infant ,Microbiology ,QR1-502 - Abstract
Abstract Objective The association between heart failure (HF) and intestinal inflammation caused by a disturbed intestinal microbiota in infants with congenital heart disease (CHD) was investigated. Methods Twenty infants with HF and CHD who were admitted to our hospital between October 2021 and March 2022 were included in this study. Twenty age- and sex-matched infants without HF at our hospital were selected as the control group. Faecal samples were obtained from each participant and analysed by enzyme-linked immunoassay and 16 S rDNA sequencing to assess intestinal inflammatory factors and the microbiota. Results The levels of intestinal inflammatory factors, including IL-1β, IL-4, IL-6, IL-17 A and TNF-α, were greatly increased, while the levels of IL-10 were significantly decreased in the HF group compared to the control group (p
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- 2024
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42. Microbiota-derived indoles alleviate intestinal inflammation and modulate microbiome by microbial cross-feeding
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Gang Wang, Yuxin Fan, Guolong Zhang, Shuang Cai, Yonghang Ma, Lijie Yang, Yuming Wang, Haitao Yu, Shiyan Qiao, and Xiangfang Zeng
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Microbial tryptophan metabolites ,Indole derivatives ,Intestinal inflammation ,Lactobacillus ,Microbial ecology ,QR100-130 - Abstract
Abstract Background The host–microbiota interaction plays a crucial role in maintaining homeostasis and disease susceptibility, and microbial tryptophan metabolites are potent modulators of host physiology. However, whether and how these metabolites mediate host–microbiota interactions, particularly in terms of inter-microbial communication, remains unclear. Results Here, we have demonstrated that indole-3-lactic acid (ILA) is a key molecule produced by Lactobacillus in protecting against intestinal inflammation and correcting microbial dysbiosis. Specifically, Lactobacillus metabolizes tryptophan into ILA, thereby augmenting the expression of key bacterial enzymes implicated in tryptophan metabolism, leading to the synthesis of other indole derivatives including indole-3-propionic acid (IPA) and indole-3-acetic acid (IAA). Notably, ILA, IPA, and IAA possess the ability to mitigate intestinal inflammation and modulate the gut microbiota in both DSS-induced and IL-10−/− spontaneous colitis models. ILA increases the abundance of tryptophan-metabolizing bacteria (e.g., Clostridium), as well as the mRNA expression of acyl-CoA dehydrogenase and indolelactate dehydrogenase in vivo and in vitro, resulting in an augmented production of IPA and IAA. Furthermore, a mutant strain of Lactobacillus fails to protect against inflammation and producing other derivatives. ILA-mediated microbial cross-feeding was microbiota-dependent and specifically enhanced indole derivatives production under conditions of dysbiosis induced by Citrobacter rodentium or DSS, but not of microbiota disruption with antibiotics. Conclusion Taken together, we highlight mechanisms by which microbiome-host crosstalk cooperatively control intestinal homoeostasis through microbiota-derived indoles mediating the inter-microbial communication. These findings may contribute to the development of microbiota-derived metabolites or targeted “postbiotic” as potential interventions for the treatment or prevention of dysbiosis-driven diseases. Video Abstract
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- 2024
- Full Text
- View/download PDF
43. Ginseng-derived nanoparticles alleviate inflammatory bowel disease via the TLR4/MAPK and p62/Nrf2/Keap1 pathways
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Song Yang, Wenjing Li, Xueyuan Bai, Giada Di Nunzio, Liangliang Fan, Yueming Zhao, Limei Ren, Ronghua Zhao, Shuai Bian, Meichen Liu, Yuchi Wei, Daqing Zhao, and Jiawen Wang
- Subjects
Ginseng-derived nanoparticles ,Intestinal inflammation ,Intestinal flora ,Oxidative stress ,Biotechnology ,TP248.13-248.65 ,Medical technology ,R855-855.5 - Abstract
Abstract Inflammatory bowel disease (IBD) is closely linked to the homeostasis of the intestinal environment, and exosomes can be used to treat IBD due to their high biocompatibility and ability to be effectively absorbed by the intestinal tract. However, Ginseng-derived nanoparticles (GDNPs) have not been studied in this context and their mechanism of action remains unclear. Here, we investigated GDNPs ability to mediate intercellular communication in a complex inflammatory microenvironment in order to treat IBD. We found that GDNPs scavenge reactive oxygen species from immune cells and intestinal epithelial cells, inhibit the expression of pro-inflammatory factors, promote the proliferation and differentiation of intestinal stem cells, as well as enhancing the diversity of the intestinal flora. GDNPs significantly stabilise the intestinal barrier thereby promoting tissue repair. Overall, we proved that GDNPs can ameliorate inflammation and oxidative stress in vivo and in vitro, acting on the TLR4/MAPK and p62/Keap1/Nrf2 pathways, and exerting an anti-inflammatory and antioxidant effect. GDNPs mitigated IBD in mice by reducing inflammatory factors and improving the intestinal environment. This study offers new evidence of the potential therapeutic effects of GDNPs in the context of IBD, providing the conceptual ground for an alternative therapeutic strategy. Graphical Abstract
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- 2024
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44. Strain specificity of lactobacilli with promoted colonization by galactooligosaccharides administration in protecting intestinal barriers during Salmonella infection
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Yujun Wu, Xiangyu Zhang, Xiaoyi Liu, Yi Li, Dandan Han, Yu Pi, Melanie A. Whitmore, Xingmiao Lu, Guolong Zhang, Jinkai Zheng, and Junjun Wang
- Subjects
Galactooligosaccharides ,Lactobacillus ,Salmonella ,Intestinal inflammation ,Medicine (General) ,R5-920 ,Science (General) ,Q1-390 - Abstract
Introduction: Galactooligosaccharides (GOS) are lactogenic prebiotics that exert health benefits by stimulating the growth of different Lactobacillus strains in the gastrointestinal (GI) tract. Objectives: This study aimed to investigate the mechanism of action of different GOS-enriched lactobacilli in intestinal health. Methods: Piglets and mice were supplemented with GOS to identify specific enrichment of Lactobacillus. The protective effects of individual GOS-enriched lactobacilli were investigated in Salmonella-infected mice. Macrophage depletion and transcriptome analysis were further performed to assess the involvement of macrophages and the underlying mechanisms of individual lactobacilli. An in vitro cell co-culture system was also used to evaluate the anti-adhesive and anti-invasive activities of lactobacilli against Salmonella in epithelial cells. Results: GOS markedly increased the relative abundance of three lactobacilli including L. delbrueckii, L. johnsonii, and L. reuteri in both piglets and mice. Supplementation with GOS further alleviated Salmonella infection in mice. L. delbrueckii (ATCC®BAA 365™), but not L. johnsonii or L. reuteri, enhanced propionate production in the intestinal tract and ameliorated Salmonella-induced intestinal inflammation and barrier dysfunction by suppressing the JAK2-STAT3 signaling and M1 macrophage polarization. L. johnsonii (BNCC 186110), on the other hand, inhibited Salmonella adhesion and invasion of epithelial cells through competitive exclusion. However, L. reuteri (BNCC 186135) failed to protect mice against Salmonella infection. Conclusion: GOS-enriched lactobacilli show a differential role in protecting against Salmonella-induced intestinal barrier dysfunction and inflammation. Our results provide novel insights into the mechanism of action of GOS and individual Lactobacillus strains in the control and prevention of intestinal inflammatory disorders.
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- 2024
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45. Epithelially Restricted Interferon Epsilon Protects Against ColitisSummary
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Eveline D. de Geus, Jennifer S. Volaric, Antony Y. Matthews, Niamh E. Mangan, Janet Chang, Joshua D. Ooi, Nicole A. de Weerd, Edward M. Giles, and Paul J. Hertzog
- Subjects
Type I Interferons ,Interferon Epsilon ,Intestinal Inflammation ,Colitis ,Diseases of the digestive system. Gastroenterology ,RC799-869 - Abstract
Background & Aims: Type I interferon (T1IFN) signalling is crucial for maintaining intestinal homeostasis. We previously found that the novel T1IFN, IFNε, is highly expressed by epithelial cells of the female reproductive tract, where it protects against pathogens. Its function has not been studied in the intestine. We hypothesize that IFNε is important in maintaining intestinal homeostasis. Methods: We characterized IFNε expression in mouse and human intestine by immunostaining and studied its function in the dextran sulfate sodium (DSS) colitis model using both genetic knockouts and neutralizing antibody. Results: We demonstrate that IFNε is expressed in human and mouse intestinal epithelium, and expression is lost in inflammation. Furthermore, we show that IFNε limits intestinal inflammation in mouse models. Regulatory T cell (Treg) frequencies were paradoxically decreased in DSS-treated IFNε-/- mice, suggesting a role for IFNε in maintaining the intestinal Treg compartment. Colitis was ameliorated by transfer of wild-type Tregs into IFNε-/- mice. This demonstrates that IFNε supports intestinal Treg function. Conclusions: Overall, we have shown IFNε expression in intestinal epithelium and its critical role in gut homeostasis. Given its known role in the female reproductive tract, we now show IFNε has a protective role across multiple mucosal surfaces.
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- 2024
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46. CRIg+ macrophages deficiency enhanced inflammation damage in IBD due to gut extracellular vesicles containing microbial DNA
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Shangshu Nie, Zhongchao Zhang, Yudong Ji, Qiang Ding, Jin Gong, Fang Xiao, Liping Chen, Dean Tian, Mei Liu, and Zhenlong Luo
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Microbial DNA ,extracellular vesicle ,CRIg+ macrophage ,IBD ,intestinal epithelial cells ,intestinal inflammation ,Diseases of the digestive system. Gastroenterology ,RC799-869 - Abstract
Gut microbiota-derived extracellular vesicles (mEVs) are reported to regulate inflammatory response by delivering bacterial products into host cells. The complement receptor of the immunoglobulin superfamily macrophages (CRIg+ Mφ) could clear invading bacteria and their derivatives. Here, we investigate the role of CRIg+ Mφ and the mechanism by which mEVs regulate intestinal inflammation. We found that it is exacerbated in IBD patients and colitis mice by mEVs’ leakage from disturbed gut microbiota, enriching microbial DNA in the intestinal mucosa. CRIg+ Mφ significantly decrease in IBD patients, allowing the spread of mEVs into the mucosa. The microbial DNA within mEVs is the key trigger for inflammation and barrier function damage. The cGAS/STING pathway is crucial in mEVs-mediated inflammatory injury. Blocking cGAS/STING signaling effectively alleviates inflammation caused by mEVs leakage and CRIg+ Mφ deficiency. Microbial DNA-containing mEVs, along with CRIg+ Mφ deficiency, stimulate inflammation in IBD, with the cGAS/STING pathway playing a crucial role.
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- 2024
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47. Relationship between heart failure and intestinal inflammation in infants with congenital heart disease.
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Zhang, Qi-Liang, Chen, Xiu-Hua, Zhou, Si-Jia, Lei, Yu-Qing, Chen, Qiang, and Cao, Hua
- Subjects
- *
CONGENITAL heart disease , *HEART failure , *INTESTINES , *GUT microbiome , *INFANTS , *BIFIDOBACTERIUM - Abstract
Objective: The association between heart failure (HF) and intestinal inflammation caused by a disturbed intestinal microbiota in infants with congenital heart disease (CHD) was investigated. Methods: Twenty infants with HF and CHD who were admitted to our hospital between October 2021 and March 2022 were included in this study. Twenty age- and sex-matched infants without HF at our hospital were selected as the control group. Faecal samples were obtained from each participant and analysed by enzyme-linked immunoassay and 16 S rDNA sequencing to assess intestinal inflammatory factors and the microbiota. Results: The levels of intestinal inflammatory factors, including IL-1β, IL-4, IL-6, IL-17 A and TNF-α, were greatly increased, while the levels of IL-10 were significantly decreased in the HF group compared to the control group (p < 0.05). The intestinal microbial diversity of patients in the HF group was markedly lower than that in the control group (p < 0.05). The abundance of Enterococcus was significantly increased in the HF group compared to the control group (p < 0.05), but the abundance of Bifidobacterium was significantly decreased in the HF group compared to the control group (p < 0.05). The diversity of the intestinal microbiota was negatively correlated with the levels of IL-1β, IL-4, IL-6 and TNF-α in the intestinal tract but was positively correlated with that of IL-10. The abundance of Enterococcus was positively associated with the levels of IL-1β, IL-4, IL-6 and TNF-α in the intestinal tract but was negatively correlated with that of IL-10. NT-proBNP was positively associated with the levels of IL-1β, IL-4, IL-6 and TNF-α in the HF group but was negatively correlated with that of IL-10. The heart function score was positively associated with the levels of IL-1β, IL-4, IL-6 and TNF-α in the HF group but was negatively correlated with that of IL-10. Conclusions: Infants with CHD-related HF had a disordered intestinal microbiota, decreased diversity of intestinal microbes, increased levels of pathogenic bacteria and decreased levels of beneficial bacteria. The increased abundance of Enterococcus and the significant decrease in the diversity of the intestinal microbiota may exacerbate the intestinal inflammatory response, which may be associated with the progression of HF. [ABSTRACT FROM AUTHOR]
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- 2024
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48. Microbiota-derived indoles alleviate intestinal inflammation and modulate microbiome by microbial cross-feeding.
- Author
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Wang, Gang, Fan, Yuxin, Zhang, Guolong, Cai, Shuang, Ma, Yonghang, Yang, Lijie, Wang, Yuming, Yu, Haitao, Qiao, Shiyan, and Zeng, Xiangfang
- Subjects
MICROBIAL metabolites ,SYNTROPHISM ,TRYPTOPHAN ,INDOLE compounds ,INTESTINES ,BACTERIAL enzymes ,INDOLE derivatives - Abstract
Background: The host–microbiota interaction plays a crucial role in maintaining homeostasis and disease susceptibility, and microbial tryptophan metabolites are potent modulators of host physiology. However, whether and how these metabolites mediate host–microbiota interactions, particularly in terms of inter-microbial communication, remains unclear. Results: Here, we have demonstrated that indole-3-lactic acid (ILA) is a key molecule produced by Lactobacillus in protecting against intestinal inflammation and correcting microbial dysbiosis. Specifically, Lactobacillus metabolizes tryptophan into ILA, thereby augmenting the expression of key bacterial enzymes implicated in tryptophan metabolism, leading to the synthesis of other indole derivatives including indole-3-propionic acid (IPA) and indole-3-acetic acid (IAA). Notably, ILA, IPA, and IAA possess the ability to mitigate intestinal inflammation and modulate the gut microbiota in both DSS-induced and IL-10
−/− spontaneous colitis models. ILA increases the abundance of tryptophan-metabolizing bacteria (e.g., Clostridium), as well as the mRNA expression of acyl-CoA dehydrogenase and indolelactate dehydrogenase in vivo and in vitro, resulting in an augmented production of IPA and IAA. Furthermore, a mutant strain of Lactobacillus fails to protect against inflammation and producing other derivatives. ILA-mediated microbial cross-feeding was microbiota-dependent and specifically enhanced indole derivatives production under conditions of dysbiosis induced by Citrobacter rodentium or DSS, but not of microbiota disruption with antibiotics. Conclusion: Taken together, we highlight mechanisms by which microbiome-host crosstalk cooperatively control intestinal homoeostasis through microbiota-derived indoles mediating the inter-microbial communication. These findings may contribute to the development of microbiota-derived metabolites or targeted "postbiotic" as potential interventions for the treatment or prevention of dysbiosis-driven diseases. FABU1xM6Tj-QxfSJJUCcEn Video Abstract [ABSTRACT FROM AUTHOR]- Published
- 2024
- Full Text
- View/download PDF
49. Postbiotics from Lactobacillus delbrueckii Alleviate Intestinal Inflammation by Promoting the Expansion of Intestinal Stem Cells in S. Typhimurium-Induced Mice.
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Wang, Mengting, Ren, Yuting, Guo, Xin, Ye, Yanxin, Zhu, Haining, Zhang, Jiaqi, Huang, Zan, and Yu, Kaifan
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LACTOBACILLUS delbrueckii ,INTESTINAL barrier function ,STEM cells ,INTESTINES ,BIFIDOBACTERIUM ,MICROBIAL diversity ,LACTOBACILLUS - Abstract
Previous studies have demonstrated that L. delbrueckii plays beneficial roles in modulating the gut microbiota, enhancing the intestinal barrier, and promoting animal growth. Postbiotics have a similar or even superior effect in protecting intestinal health compared to probiotics due to their excellent stability, extended shelf life, and safety. However, the protective effects and underlying mechanism of postbiotics from L. delbrueckii in intestinal inflammation remain unclear. In this study, we demonstrated the beneficial impact of postbiotics from L. delbrueckii on intestinal health by establishing a S. Typhimurium-induced intestinal inflammation model in mice, which included inactivated bacteria and supernatant. The results revealed that the probiotics and postbiotics from L. delbrueckii increased the survival rate and body weight of S. Typhimurium-induced mice, increased the level of IL-10, and decreased the levels of TNF-α and IL-6, thereby alleviating intestinal inflammation. Meanwhile, treatment with postbiotics decreased the levels of D-LA, DAO, and LPS and promoted the expression of Occludin, ZO-1, and Claudin-1 in the serum and jejunum, suggesting an improvement in intestinal barrier function by postbiotics. Additionally, the postbiotics modulated gut microbial diversity, increased the ratio of Firmicutes and Bacteroidetes, and restored the abundance of Muribaculaceae, Lachnospiraceae_NK4a136_groups, and Alloprevotella in S. Typhimurium-infected mice. Moreover, postbiotics from L. delbrueckii promoted the expansion of intestinal stem cells (ISCs) and increased the numbers of Paneth and Goblet cells. Taken together, these data revealed the beneficial role of postbiotics from L. delbrueckii in protecting against intestinal inflammation by promoting the expansion of ISCs. [ABSTRACT FROM AUTHOR]
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- 2024
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50. Pyroptosis: a new insight into intestinal inflammation and cancer.
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Limin Chao, Wenjing Zhang, Yuchao Feng, Pei Gao, and Jinyou Ma
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INTESTINAL cancer ,PYROPTOSIS ,INTESTINAL diseases ,INFLAMMASOMES ,IMMUNE response - Abstract
Pyroptosis is an innate immune response triggered by the activation of inflammasomes by various influencing factors, characterized by cell destruction. It impacts the immune system and cancer immunotherapy. In recent years, the roles of pyroptosis and inflammasomes in intestinal inflammation and cancer have been continuously confirmed. This article reviews the latest progress in pyroptosis mechanisms, new discoveries of inflammasomes, mutual regulation between inflammasomes, and their applications in intestinal diseases. Additionally, potential synergistic treatment mechanisms of intestinal diseases with pyroptosis are summarized, and challenges and future directions are discussed, providing new ideas for pyroptosis therapy. [ABSTRACT FROM AUTHOR]
- Published
- 2024
- Full Text
- View/download PDF
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