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2 results on '"heat shock protein 70kd"'

1. Immunogenicity of the Recombinant Cryptococcus neoformans HSP70, a Potential Candidate for Developing an ELISA Kit

Author

Shima Safaei, Hossein Rezvan, Roohollah Fateh, and Mohammad Khalifeh Gholi

Subjects
cryptococcus neoformans, enzyme-linked immunosorbent assay, heat shock protein 70kd, Pathology, RB1-214
Abstract

Introduction: Cryptococcus neoformans is an encapsulated fungal pathogen that causes life-threatening meningoencephalitis in immunocompromised patients. This yeast secretes several potent immunogenic proteins by secretory vesicular mechanisms, such as HSP70 chaperone. Methods: The PCR-amplified HSP70 gene was cloned into a PET-28a(+) expression vector. The purified recombinant HSP70 (rHSP70) was evaluated by western blotting using an anti-His Tag-HRP antibody and then used for immunization of a rabbit. The serum of the immunized rabbit was tested against the whole lysate of C. neoformans in ELISA. Results: The antibodies in the rabbit’s serum recognized lysate of C. neoformans yeast. The highest antibody levels were achieved after the third booster injection. Conclusion: The rHSP70 showed to be a reliable candidate for the designing and development of an ELISA kit for early detection of cryptococcosis, and to screen a large number of specimens.

Published
2020

2. Immunogenicity of the Recombinant Cryptococcus neoformans HSP70, a Potential Candidate for Developing an ELISA Kit

Author

Roohollah Fateh, Mohammad Khalifeh Gholi, Hossein Rezvan, and Shima Safaei

Subjects
Cryptococcus neoformans, cryptococcus neoformans, Animal health, Immunogenicity, Potential candidate, heat shock protein 70kd, Biology, biology.organism_classification, Virology, Hsp70, law.invention, Elisa kit, law, Recombinant DNA, lcsh:Pathology, Vector (molecular biology), enzyme-linked immunosorbent assay, lcsh:RB1-214
Abstract

Introduction: Cryptococcus neoformans is an encapsulated fungal pathogen that causes life-threatening meningoencephalitis in immunocompromised patients. This yeast secretes several potent immunogenic proteins by secretory vesicular mechanisms, such as HSP70 chaperone. Methods: The PCR-amplified HSP70 gene was cloned into a PET-28a(+) expression vector. The purified recombinant HSP70 (rHSP70) was evaluated by western blotting using an anti-His Tag-HRP antibody and then used for immunization of a rabbit. The serum of the immunized rabbit was tested against the whole lysate of C. neoformans in ELISA. Results: The antibodies in the rabbit’s serum recognized lysate of C. neoformans yeast. The highest antibody levels were achieved after the third booster injection. Conclusion: The rHSP70 showed to be a reliable candidate for the designing and development of an ELISA kit for early detection of cryptococcosis, and to screen a large number of specimens.

Published
2020

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