1. Natural Variation of Hazelnut Allergenicity: Is There Any Potential for Selecting Hypoallergenic Varieties?
- Author
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Gilberto Igrejas, Sandra Cabo, Ana Paula Silva, Berta Gonçalves, Mélanie Hillion, Michel Hébraud, Joana Costa, Isabel Mafra, Miguel Ribeiro, Universidade de Trás-os-Montes e Alto Douro (UTAD), Universidade do Trás-os-Montes e Alto Douro, LAQV-REQUIMTE, Universidade NOVA de Lisboa, Departamento de Quimica (REQUIMTE), Universidade de Lisboa (ULISBOA)-Centro de Quimica Fina e Biotecnologia, Centro de Investigação e de Tecnologias Agro-Ambientais e Biológicas (CITAB), University of Trás-os-Montes and Alto Douro [Portugal] (UTAD), INRAE, Plateforme d’Exploration du Métabolisme, Composante Protéomique (PFEMcp), 63122 Saint-Genès Champanelle, Microbiologie Environnement Digestif Santé (MEDIS), Université Clermont Auvergne [2017-2020] (UCA [2017-2020])-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Associate Laboratory for Green Chemistry-LAQV - Fundacao para a Ciencia e Tecnologia (FCT) UIDB 50006/2020national project AlleRiskAssess PTDC/BAA-AGR/31720/2017NORTE-01-0145-FEDER-00001national project HazelOmics (LAQV-REQUIMTE) INTERACT project-'Integrative Research in Environment, Agro-Chains and Technology' NORTE-01-0145-FEDER-000017European Union (EU), Universidade Nova de Lisboa = NOVA University Lisbon (NOVA), Universidade de Lisboa = University of Lisbon (ULISBOA)-Centro de Quimica Fina e Biotecnologia, Plateforme Exploration du Métabolisme (PFEM), Institut National de la Recherche Agronomique (INRA)-Université Blaise Pascal - Clermont-Ferrand 2 (UBP)-MetaboHUB-Clermont, and MetaboHUB-MetaboHUB
- Subjects
0301 basic medicine ,Male ,Proteomics ,medicine.medical_treatment ,0302 clinical medicine ,immunoblot ,Exclusion diet ,Food science ,Desensitization (medicine) ,Plant Proteins ,2. Zero hunger ,Nutrition and Dietetics ,genetic diversity ,respiratory system ,Middle Aged ,[SDV.MP]Life Sciences [q-bio]/Microbiology and Parasitology ,nutrition ,Hazelnut allergy ,Child, Preschool ,proteogenomics ,Female ,Nut Hypersensitivity ,lcsh:Nutrition. Foods and food supply ,Food Hypersensitivity ,Adult ,Adolescent ,lcsh:TX341-641 ,Biology ,Natural variation ,hazelnut-allergic patients’ sera ,Article ,03 medical and health sciences ,Young Adult ,Corylus ,Food allergy ,medicine ,Humans ,hazelnut allergy ,Aged ,Genetic diversity ,Genetic Variation ,Hypoallergenic ,Allergens ,Antigens, Plant ,Immunoglobulin E ,medicine.disease ,hazelnut-allergic patients' sera ,respiratory tract diseases ,030104 developmental biology ,Human nutrition ,030228 respiratory system ,Food Science - Abstract
International audience; Hazelnuts (Corylus avellanaL.) have an important role in human nutrition and health. However, they are a common cause of food allergy. Due to hazelnut varietal diversity, variety-dependent differences in the IgE-binding properties may be suspected, which could allow therapeutic strategies based on the use of hypoallergenic varieties to induce desensitization. In a proteogenomic approach, we aimed to evaluate the allergenic potential of a genetically diverse set of hazelnuts (n = 13 varieties). Minor differences were found at the level of genes encoding important allergens, namely Cor a 8, Cor a 9, and Cor a 14. Nevertheless, IgE-reactivity was similar for all varieties using sera from seven allergic individuals. The predominant IgE-reactive proteins were Cor a 9 (100%) and Cor a 1.04 (60%), with the former being the most frequently identified by a two-dimensional gel electrophoresis (2-DE)-based proteomic approach. Therefore, it seems that the conventional exclusion diet will hold its ground for the time being.
- Published
- 2020
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