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Biosurfactants are ampliphlic compounds which can reduce the surface and interfacial tension between the compounds and have multiuses in various industries and agriculture. The isolated lipopeptides and lipoprotein producing microbes are useful in removal of heavy metals from industrial effluents using bioremediation, pesticides and hydrocarbon contaminated sites. Lipopeptides and lipoproteins used as bio-control agent to protect plant against different diseases, leads in increases crop yield. The objective was to isolate potential bio surfactant producing microbes from hydrocarbons contaminated soil. Initially 21 microbes were isolated amidst them ten bio surfactant producers were screened based on the surface tension, emulsification index, drop collapse method and oil displacement method. The isolate KLEF21 was the most successful bio surfactant producer as it showed the minimum surface tension (32.12 dyne/cm) with an emulsification index of 68.97% and oil displacement activity 3.5 mm. The microbe was identified based on the partial sequence of 16S rRNA and it belongs to Alcaligenes and was indicated as Alcaligenes sp.KLEF21.The stain was also competent of employ polycyclic aromatic hydrocarbon composed of three fused benzene rings as the highest microbes biomass was produced by this strain in polycyclic aromatic hydrocarbon alter MSM media compared to other isolates.

Background: Achromobacter xylos strain GSR21 plays a crucial role in bioremediation of fossil fuel contamination, biopharmaceutical, cosmetics, chemical, petroleum refining, petrochemical, food industries and tertiary oil recovery (MEOR). Aim: within the present paper, to reinforce the censorious medium constituents for the assembly of Achromobacter xylos strain GSR21 by using response surface quadratic models (RSQM). Materials and methods: Response surface method (RSM) was utilized to make your mind up the best degrees of cycle factors (agar powder, yeast concentrate, FeSO4.7H2O, and KH2PO4). CCD design of RSM was utilized to contemplate the four factors at five levels, and Achromobacter xylos strain GSR21 fixation was estimated as reaction. Results: Relapse coefficients were dictated by relapse examination, and therefore the model condition was settled. R2 regard for bio-surfactant (g/L) was attempted to be 0.88, showing that the model fitted well with the exploratory results. Affirmation of the mathematical model was driven by playing out the examination with the foreseen updated values, and bio-surfactant yield was found to be 9.88 g/L. Endorsement of the foreseen model was fitted 98.8% with the test outcomes coordinated under the perfect conditions. Conclusion: In light of the above outcomes agar powder and yeast separate was perceived as compelling fragments for Achromobacter xylos GSR21 creation.

Achromobacter xylos strain GSR21 plays a crucial role in bioremediation of fossil fuel contamination, biopharmaceutical, cosmetics, chemical, petroleum refining, petrochemical, food industries and tertiary oil recovery (Microbial enhanced oil recovery). Response surface quadratic models (RSQM) was applied to reinforce the censorious operating conditions for the assembly of Achromobacter xylos strain GSR21. The Response surface method (RSM) was application to determine the best degrees of cycle factors (Temperature, Concentration, RPM, pH). Central composite design (CCD) of RSM was used to contemplate the four factors at five levels, and strain GSR21 Achromobacter xylos fixation was approximate as a reaction. Relapse coefficients predicted by examination and therefore the model was settled. R2 value regard for bio-surfactant (mN/m) attempted to be 0.81, showing that the model fitted well with the explorative results. The mathematical model predicted by simulation of the foreseen updated values, and bio-surfactant surface tension was found 50 mN/m. The foreseen model was matched at 98.8% with the test outcomes coordinated under the perfect conditions. Based on the finding research, temperature-40°C, concentration-1.8 g/l, RPM-180 rev/mint and pH-4 was perceived as compelling fragments for Achromobacter xylos GSR21.

The purpose of this study was to explore the different roles of heat shock proteins especially in immunity and infections. Heat shock proteins were discovered 56 years ago, and much of the work has focused on the role of these proteins that play in protecting cells from stress. Heat shock proteins are highly conserved class of proteins present in all species from bacteria to humans. Several experimental studies have been successful in pointing to the role of heat shock protein as a clinical biomarker and therapeutic target in a variety of diseases. It will also highlight features of HSP family, and will discuss future implications of HSPs in the diagnosis and prognosis of clinical and therapeutic significance. The development of membrane-interacting drugs that modify specific membrane domains, modulating heat shock response, may also be of significant therapeutic benefit. These proteins function as molecular chaperones, assisting in the refolding of misfolded proteins or their elimination if they become irreversibly damaged. Proteomic studies have identified several different HSPs in various disease types that may be clinical biomarkers or molecular targets for various therapies.

Thermo bodily residences of binary liquid combos are very beneficial in biotechnology, pharmaceutical, chemical and petroleum refining industries for improved favoured products from diverse raw materials. The physical property data on mixed solvents are important for the theoretical and applied areas of research and are frequently used in many chemical and industrial processes such as design of new process and process equipment (fluid flow, mass transfer or heat transfer calculation) and designing of bioreactor/fermenter. The objective of the current paper is to determine the density, viscosity, ultrasonic velocity, refractive index and surface tension of acetone (1) + water (2) at temperatures of 303.15K to 318.15K for the complete composition degrees and atmospheric pressure. These experimental values were used to calculate the respective excess homes in conjunction with a few acoustic properties. The experimental facts and excess residences have been used to calculate the interacting coefficients and well known deviations from different existing models. Also the new model equations have been evolved with the aid of the use of Design Expert application for density, viscosity, ultrasonic velocity, refractive indices and surface tension. Experimental consequences have been analysed on the premise of molecular interactions among element molecules with the assistance of FT-IR spectrum. Acetone-water binary structures of the prevailing observer have positive values of excess molar volumes and deviations in isentropic compressibility over whole composition range and at all temperatures which suggests sturdy interactions among the components of binary mixtures. Thermodynamic observations of acetone and water mixtures were made. Negative viscosity deviation of acetone-water combinations suggests robust dipole-dipole interactions within the device. Fourier remodel infrared spectroscopy also indicates the sturdy interaction made at 3589 cm-1 and based on the response surface method outcomes, more interplay takes area at zero. Five and zero six moles of acetone and water combination and the consequences are conformed with reference to the R2 cost (0.89). Excess molar extent and isentropic compressibility were decided and kinds of interactions have been discussed primarily based on the derived properties.

Biosurfactants plays a key role in tertiary recovery (EOR), and production, excessive minerals discharge from water during petroleum refinery process, environmental utilization and eco-friendly. Biosurfactant producing bacteria found to be sufficient in hydrocarbon-polluted soil samples; it is expected to more amounts of agrichemicals contemporary in the clay. These bacteria establish itself soil and region specific. So, in this study we made an attempt to identify and characterize biosurfactant produced by achromobacter xylos strain GSR21 from hydrocarbon polluted soil in Andhrapradesh, India. A battery of biosurfactant screening methods engaged were haemolytic activity, oil spreading technique, lipase activity, emulsification index (E24), emulsification assay, tilting glass slide, blood haemolysis test, drop-collapsed assay, and foaming activity. The organism isolate was studied based on molecular, phenotypic, and biochemical methods. Thin-layer chromatography I(TLC), Fourier transforms infrared spectroscopy (FTIR) and gas chromatography-mass spectrometry (GC–MS) investigates were used to identify and characterize the biosurfactant produced. The isolated biosurfactant was applied on chosen hydrocarbons to measure its emulsifying capacity.The phylogeny study of the 16S rRNA classified the isolate as A chromobacter xylos strain GSR21. The sequence secured from the isolate has been accumulated in GenBank covered by the accession number JQ746488. The result obtained from the study acknowledge high biosurfactant action with a maximum emulsification index (E24) of 62 % compared to emulsification index (E24) of 72% by sodium lauryl sulfate (SLS). Moreover, the biosurfactant appear emulsifying activity against the following hydrocarbons: diesel, methylbenzene, kerosene, dimethylbenzene, and petrol. The optimum cultural conditions (incubation time, carbon, pH, hydrocarbon, inoculum concentration, nitrogen, and temperature) for growth and biosurfactant produced by A chromobacter xylos GSR21 were analysed. The biosurfactant was characterized as a glycolipid using thin layer chromatography (TLC), while the gas chromatography-mass spectrometry (GC–MS) technique analyzed the glycolipid as dodecanoic acid-undecyl ester.Present study has exhibited the magnitude of Achromobacter xylos strain GSR21 isolated from hydrocarbon-polluted soil to produce biosurfactant and the effectiveness of the produced biosurfactant in emulsifying different hydrocarbons. Moreover, the biosurfactant produced was established to be held by the class, glycolipid based on the thin layer chromatography (TLC) and gas chromatography-mass spectrometry (GC–MS) analyses.

Introduction: Biosurfactants have picked up an impressive consideration as of late due to their potential uses in an expansive scope of use territories, including environmental remediation, agriculture, biofilm formation, quorum sensing, textile, pharmaceuticals, cosmetics, and the food, oil, and petrochemical industries. Aim: In the present study, optimization of the critical medium components for biosurfactant production by Achromobacter xylos strain GSR21 using statistical experimental design was studied. Materials and Methods: Response surface methodology (RSM) was employed to determine the optimal level of the four medium variables (agar powder, yeast extract, FeSO47H2O, and KH2PO4). Central composite design of RSM was applied to study the four variables at five levels, and biosurfactant concentration was measured as a response. Results: Regression coefficients were calculated by regression analysis and the model equation was determined. R2 value for biosurfactant (g/L) was calculated as 72%, and it indicates that the model was well fitted with the experimental results. Surface plots were made, and the maximum biosurfactant production (A. xylos strain GSR21) (10.20 g/L) was predicted at the optimized values of agar powder 90 g/L, yeast extract 5 g/L, FeSO47H2O 0.05 g/L, and KH2PO4 0.15 g/L. The obtained mathematical model was verified by performing the experiment with the predicted optimized values, and the yield of bio-surfactant was found to be 9.69 g/L. Validation of the predicted model was fitted 96.9% with the experimental results conducted at the optimum conditions. Conclusion: Results of this statistical analysis showed that agar powder and yeast extract had found significant medium components for biosurfactant (A. xylos GSR21) production.

This paper, for the first time, reports the optimization of the critical medium components for bio-surfactant production from achromobacter xylos strain GSR21 using statistical experimental design. Response surface methodology (RSM) was employed to determine the optimal levels of process variables (agar powder, yeast extract, FeSO 4 7H 2 O, and KH 2 PO 4 ). Central composite design (CCD) of RSM was used to study the four variables at five levels, and bio-surfactant concentration was measured as response. Regression coefficients were calculated by regression analysis, and the model equation was determined. R 2 value for bio-surfactant (g/L) was tested to be 0.7222, indicating that the model fitted well with the experimental results. Verification of the mathematical model was conducted by performing the experiment with the predicted optimized values, and bio-surfactant yield was found to be 9.69 g/L. Validation of the predicted model was fitted 96.9% with the experimental results conducted under the optimum conditions. Agar powder and yeast extract was identified as efficient components for bio-surfactant ( achromobacter xylos GSR21) production.

Copyright: 2014. Wiley Online Library. Due to copyright restrictions, only the abstract is available. For access to the full text item, please consult the publisher's website. The definitive version of the work is published in Journal of Biomedical Materials Research Part A, Vol 102, No.12, 4616-4624 http://onlinelibrary.wiley.com/doi/10.1002/jbm.a.35136/abstract

Density, viscosity, refractive index, ultrasonic velocity and surface tension values have been determined at temperature 313.15 K for the whole compositions for the binary liquid mixtures of acetone, hexane, heptane and dodecane with water at atmospheric pressure. These experimental values have been used to estimate the respective excess properties along with some acoustic and thermodynamics properties. From the properties of these excess parameters the nature and quality of the interactions and solubilisation potential and thermodynamic stability these binary systems are discussed and finally we are identified the best organic solvent for microencapsulation technique.