1. Fecal Protease Activity Is Associated with Compositional Alterations in the Intestinal Microbiota
- Author
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Tamar Ringel-Kulka, Jennica P. Siddle, Yehuda Ringel, Lionel Bueno, Michael C. Wu, Ian M. Carroll, Laurent Ferrier, North Carolina State University, Center for High Performance Simulation and Department of Chemical and Biomolecular Engineering, ToxAlim (ToxAlim), Université Toulouse III - Paul Sabatier (UT3), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Institut National Polytechnique (Toulouse) (Toulouse INP), Université Fédérale Toulouse Midi-Pyrénées-Ecole Nationale Vétérinaire de Toulouse (ENVT), Institut National Polytechnique (Toulouse) (Toulouse INP), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Ecole d'Ingénieurs de Purpan (INPT - EI Purpan), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Institut National de la Recherche Agronomique (INRA), University of North Carolina, young investigator grant for probiotics research, global probiotics committee, National Institutes of Health [DK084294, DK075621], and [DK092330]
- Subjects
Male ,IRRITABLE-BOWEL-SYNDROME ,[SDV]Life Sciences [q-bio] ,medicine.medical_treatment ,STREPTOCOCCUS-PNEUMONIAE ,lcsh:Medicine ,PROTEINASE-ACTIVATED RECEPTOR-2 ,ULCERATIVE-COLITIS ,PERMEABILITY ,DISEASES ,PAIN ,SENSITIVITY ,PROTEOLYSIS ,MICROFLORA ,Feces ,0302 clinical medicine ,Lactobacillales ,RNA, Ribosomal, 16S ,lcsh:Science ,0303 health sciences ,Multidisciplinary ,biology ,Intestines ,population characteristics ,030211 gastroenterology & hepatology ,Female ,geographic locations ,Research Article ,Adult ,DNA, Bacterial ,Proteases ,Streptococcaceae ,Microbiology ,03 medical and health sciences ,parasitic diseases ,medicine ,Humans ,Microbiome ,030304 developmental biology ,Protease ,Ruminococcus ,Lachnospiraceae ,lcsh:R ,social sciences ,biology.organism_classification ,lcsh:Q ,human activities ,Peptide Hydrolases - Abstract
Objective Intestinal proteases carry out a variety of functions in the gastrointestinal (GI) tract. Studies have reported that elevated enteric proteases in patients with GI disease can alter intestinal physiology, however the origin (human vs. microbial) of elevated proteases in patients with GI disease is unclear. Aim The aim of this study was to investigate the association between protease activity and the microbiota in human fecal samples. Design In order to capture a wide range of fecal protease (FP) activity stool samples were collected from 30 IBS patients and 24 healthy controls. The intestinal microbiota was characterized using 454 high throughput pyro-sequencing of the 16S rRNA gene. The composition and diversity of microbial communities were determined and compared using the Quantitative Insights Into Microbial Ecology (QIIME) pipeline. FP activity levels were determined using an ELISA-based method. FP activity was ranked and top and bottom quartiles (n=13 per quartile) were identified as having high and low FP activity, respectively. Results The overall diversity of the intestinal microbiota displayed significant clustering separation (p = 0.001) between samples with high vs. low FP activity. The Lactobacillales, Lachnospiraceae, and Streptococcaceae groups were positively associated with FP activity across the entire study population, whilst the Ruminococcaceae family and an unclassified Coriobacteriales family were negatively associated with FP activity. Conclusions These data demonstrate significant associations between specific intestinal bacterial groups and fecal protease activity and provide a basis for further causative studies investigating the role of enteric microbes and GI diseases.
- Published
- 2013
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