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Unlike wild giraffe that primarily consume low starch browse, the preference of zoo‐housed giraffe for consuming supplemental feeds over forage could increase the risk of digestive disorders such as ruminal acidosis. Our objective was to evaluate the effects of modifying a supplemental feed's non‐fibre carbohydrate profile and physical form on nutritional, behavioural, and blood measures of giraffe in a zoological setting. Six non‐lactating, adult, female reticulated giraffes were used in a two‐pen modified reversal study using two dietary treatments in seven 21‐day periods with data collected on days 15–21. Dietary treatments were a control feed comprised of commercially available products used at the time as the giraffe feed (GF) and an unpelleted experimental feed (EF). On a dry matter basis, GF and EF, respectively, contained 17.0% and 17.4% crude protein, 14.2% and 1.5% starch, 14.9% and 21.3% ethanol‐soluble carbohydrates, 22.9% and 26.0% acid detergent fibre (ADF) and 9.50% and 14.9% ND‐soluble fibre (NDSF), with modulus of fineness values of 3.62 and 4.82. Supplemental feeds, alfalfa hay, salt, and water were available for ad libitum consumption. Significance was declared at p ≤ 0.05. Intakes of hay, supplemental feeds, and total feed did not differ by diet (p > 0.28), though intakes of starch (0.93 and 0.12 kg; p = 0.05) and ADF (1.83 and 2.23 kg; p = 0.04) differed between GF and EF respectively. Giraffe behaviour values (min/48 h) were greater with EF for total eating (p = 0.04); diets were not detected as different for engagement in oral stereotypes (GF = 433, EF = 318 min/48 h; p = 0.22). Blood glucose was higher on GF than EF (99.0 and 82.3 mg/dL; p = 0.03). The lower EF blood glucose value is more similar to ranges reported for domesticated ruminants. No differences were detected for changes in body weight or body condition score in the 21‐day periods (p > 0.32). Modification of supplemental feed carbohydrate profile and physical form can influence behaviour and blood glucose values of zoo‐housed giraffe. [ABSTRACT FROM AUTHOR]

Giraffe is the tallest terrestrial mammal in the world, which has high ornamental value in the zoo. In recent years, the size and number of giraffes have dropped sharply, and they have been rated as vulnerable (VU) species by International Union for Conservation of Nature (IUCN). Taiyuan Zoo has accumulated some successful experiences in the process of raising giraffes for many years. Based on the long-term observation of giraffe's daily behavior, the key technologies of giraffe's daily feeding and disease prevention were summarized, and how to feed giraffe better was discussed, so as to provide references for giraffe's breeding and disease prevention in zoo. [ABSTRACT FROM AUTHOR]

Lumpy skin disease virus (LSDV) has recently undergone rapid spread, now being reported from more than 80 countries, affecting predominantly cattle and to a lesser extent, water buffalo. This poxvirus was previously considered to be highly host-range restricted. However, there is an increasing number of published reports on the detection of the virus from different game animal species. The virus has not only been shown to infect a wide range of game species under experimental conditions, but has also been naturally detected in oryx, giraffe, camels and gazelle. In addition, clinical lumpy skin disease has previously been described in springbok (Antidorcas marsupialis), an African antelope species, in South Africa. This report describes the characterization of lumpy skin disease virus belonging to cluster 1.2, from field samples from springbok, impala (Aepyceros melampus) and a giraffe (Giraffa camelopardalis) in South Africa using PCR, Sanger and whole genome sequencing. Most of these samples were submitted from wild animals in nature reserves or game parks, indicating that the disease is not restricted to captive-bred animals on game farms or zoological gardens. The potential role of wildlife species in the transmission and maintenance of LSDV is further discussed and requires continuing investigation, as the virus and disease may pose a serious threat to endangered species. [ABSTRACT FROM AUTHOR]

The diurnal activity budgets of giraffes were effectively studied from June 2012 until August 2013, in the Kalahari Region of southern Africa. Direct field observations identified how the daily activity budgets varied amongst the different subgroups of giraffes and the effects of seasonal changes and the time of the day. Feeding was the highest-performed activity whilst non-feeding activities were, however, also prominent. This study aimed to determine why giraffes are willing to sacrifice time on nonfeeding activities. A deeper look into the social and non-feeding-related behaviour of giraffes might prove that giraffes devote significant time to various other activities to improve their health and comfort. It unveils trade-offs between digestion efficiency and foraging, impacting energy gain. Understanding this balance informs conservation strategies, habitat management and their well-being. Investigating the motivations behind these behavioural choices unveils adaptive mechanisms, shedding light on the intricate interplay between physiological and ecological needs, ultimately contributing to a holistic comprehension of giraffe's ecological roles and promoting their coexistence with their environment. Such investigations also aid in highlighting why giraffes' social structures should be considered as complex and why further research is needed to better understand these relationships, amongst subspecies. [ABSTRACT FROM AUTHOR]

Consumers tend to buy meat based on visual physical characteristics, which are affected by the chemical composition of the meat, and there is very little known about the chemical composition of the meat of giraffe. This study therefore aims to broaden the knowledge base on the chemical composition of giraffe meat, rib bone and liver. Eight different muscles from 15 giraffes were analyzed to determine the chemical composition, yielding an average moisture of 77.2 ± 0.09 g/100 g meat, an average protein of 20.8 ± 0.09 g/100 g meat, an average intramuscular fat (IMF) of 1.4 ± 0.03 g/100 g meat and an average ash of 1.1 ± 0.01 g/100 g meat. There was a significant interaction between sex and muscle for the moisture, protein and ash contents, while only muscle had an effect on the fat content. The mineral content of the bone, liver and Longissimus thoracis et lumborum muscle was also analyzed, and bone was found to be a rich source of calcium (highest concentration), whilst the liver had the highest concentration of iron. The chemical composition of the giraffe meat was such that it could be classified as lean meat. [ABSTRACT FROM AUTHOR]

Immunohistochemistry staining is an essential method in pathological diagnoses. Podoplanin (PDPN) is a specific maker of alveolar epithelium, lymphatic vessels, and glomeruli. In this study, we established a novel anti-giraffe PDPN (girPDPN) mAb, PMab-301, using the Cell-Based Immunization and Screening (CBIS) method. PMab-301 (mouse IgG1, kappa) detected girPDPN in various applications, such as flow cytometry, western blot, and immunohistochemistry. PMab-301 specifically stained type-I alveolar cells using formalin-fixed paraffin-embedded giraffe lung tissues. Our findings suggest the potential usefulness of PMab-301 for the pathophysiological analyses of giraffe tissues. [ABSTRACT FROM AUTHOR]

Measurement of zoo animal welfare states enables improvement to husbandry and management to be evidence-based and implemented according to species’ needs. Theoretical welfare concepts are often discussed, and whilst it is helpful to ensure wide consensus across all stakeholders on what welfare comprises, practical application of such evidence-based information is as equally important. All species housed in zoos will have specific needs that must be met by their housing, husbandry, and care to enable them to thrive. Therefore, this paper examined how to identify key animal care needs and an animal’s responses to them to form a basis for species-specific welfare assessment approaches. There are examples of familiar-to-the-zoo species that still pose challenges regarding delivery of optimal husbandry and management. As such, the identification and evaluation of core concepts of the biology, behaviour, “needs and wants” of these species is required to support validation and refinement of physical, behavioural, and psychological welfare measures. This article evaluated the use of evidence to build capacity in welfare measurement for a familiar zoo-housed species, the giraffe (Giraffa camelopardalis, Linnaeus 1758), by outlining seven key needs and requirements that must be provided in the zoo (“giraffe W-E-L-F-A-R-E” = Warmth, Enrichment, Leaves, Feeding, Alfalfa, Rumination, Exercise). Provision of these inputs, and opportunities for all giraffes in a herd to engage with them, provides the foundation for further welfare assessment to be implemented. Specifically, the validation and measurement of mental states that are more likely to be positive if key behavioural and ecological needs have already been met. This paper advocated for this evidence-based approach to “welfare-focussed husbandry”, with distillation of key information that supports species-relevant care, to be developed for other zoo-housed species as support for their welfare assessment protocols. Such welfare-focussed husbandry is layered on top of the basic animal care requirements of the species in the zoo to ensure all individuals have the best opportunity to attain positive welfare states. In this way, and once validated, foundational welfare assessment can be easily completed by busy animal care staff, capacity is built into zoo operations as all stakeholders are aware of exactly what each species needs, and deeper dive welfare assessment (especially concerning animal mental states) can be targeted more effectively. Further evolution of these seven steps for the giraffe is suggested and extrapolation of this approach, to aid identification of key welfare indicators across all zoo-housed species, is encouraged.

Unsustainable hunting, both illegal and legal, has led to the extirpation of many species. In the last 35 years giraffe Giraffa spp. populations have declined precipitously, with extinctions documented in seven African countries. Amongst the various reasons for these population declines, poaching is believed to play an important role in some areas. Giraffes are primarily hunted for consumption and for the use of their body parts as trophies and in traditional medicine. However, the socio-economic factors that correlate with the use of giraffe body parts are not well understood. We conducted our study in Tsavo Conservation Area, Kenya, which experiences high levels of poaching. We used semi-structured surveys amongst 331 households to document how giraffe body parts are typically acquired and their intended use (i.e. trophy, medicinal or consumptive). We then used logistic regression models to assess the correlations between nine socio-economic factors and the use of giraffe body parts. We found that giraffe body parts had mostly consumptive and trophy uses. One-time suppliers, opportunistic access and widely known markets were the most common means of acquiring giraffe body parts. Results from our models showed that three variables (gender: men, occupation: tourism worker, and land ownership) were correlated significantly and positively with the use of giraffe body parts. We describe the complex links between socio-economic factors and the use of giraffe body parts and highlight the importance of implementing mitigation measures adapted to local contexts to combat a challenge that many species of conservation concern are facing.

Measurement of zoo animal welfare states enables improvement to husbandry and management to be evidence-based and implemented according to species' needs. Theoretical welfare concepts are often discussed, and whilst it is helpful to ensure wide consensus across all stakeholders on what welfare comprises, practical application of such evidence-based information is as equally important. All species housed in zoos will have specific needs that must be met by their housing, husbandry, and care to enable them to thrive. Therefore, this paper examined how to identify key animal care needs and an animal's responses to them to form a basis for species-specific welfare assessment approaches. There are examples of familiar-to-the-zoo species that still pose challenges regarding delivery of optimal husbandry and management. As such, the identification and evaluation of core concepts of the biology, behaviour, "needs and wants" of these species is required to support validation and refinement of physical, behavioural, and psychological welfare measures. This article evaluated the use of evidence to build capacity in welfare measurement for a familiar zoo-housed species, the giraffe (Giraffa camelopardalis, Linnaeus 1758), by outlining seven key needs and requirements that must be provided in the zoo ("giraffe W-E-L-F-A-R-E" = Warmth, Enrichment, Leaves, Feeding, Alfalfa, Rumination, Exercise). Provision of these inputs, and opportunities for all giraffes in a herd to engage with them, provides the foundation for further welfare assessment to be implemented. Specifically, the validation and measurement of mental states that are more likely to be positive if key behavioural and ecological needs have already been met. This paper advocated for this evidence-based approach to "welfare-focussed husbandry", with distillation of key information that supports species-relevant care, to be developed for other zoo-housed species as support for their welfare assessment protocols. Such welfare-focussed husbandry is layered on top of the basic animal care requirements of the species in the zoo to ensure all individuals have the best opportunity to attain positive welfare states. In this way, and once validated, foundational welfare assessment can be easily completed by busy animal care staff, capacity is built into zoo operations as all stakeholders are aware of exactly what each species needs, and deeper dive welfare assessment (especially concerning animal mental states) can be targeted more effectively. Further evolution of these seven steps for the giraffe is suggested and extrapolation of this approach, to aid identification of key welfare indicators across all zoo-housed species, is encouraged. [ABSTRACT FROM AUTHOR]

The distinctive long neck of the giraffe (Giraffa camelopardalis) entails functional difficulties brought about by the extended distance between the heart and the head. Blood must be circulated over 2 m from the heart to the brain against gravitational force. The natural movement of the head to ground level would result in a large volume of blood moving toward the brain with the force of gravity. Large blood volumes also rush to the brain during bulls’ fighting (necking), rendering the giraffe susceptible to possible brain damage. The natural movement of the head from ground level to fully erect would result in blood moving away from the brain with gravitational force. The lack of blood perfusing the brain can cause fainting. The giraffe, however, suffers neither brain damage nor fainting. What adaptations do giraffes have to counteract these challenges? The aim of this study was to investigate the functionality of the rostral epidural rete mirabile situated just beneath the brain and its possible contribution to successful circulation in long-necked giraffes. The unique rostral epidural rete mirabile structure significantly contributes to counteract physiological challenges. Turns and bends characterize this structural arterial meshwork and subsequently an increased artery length through which blood flow must proceed before entrance into the brain, exerting resistance to blood racing to the brain when the head is lowered to the ground. The brain is supplied mainly by the maxillary artery through the carotid rete, with a rudimentary basilar artery not contributing to the brain’s blood supply. The resistance to blood flow due to the structure and position of the rostral epidural rete mirabile when the head is in the upright position is counteracted by the unique carotid-vertebral anastomosis allowing immediate cerebral blood supply. The rostral epidural rete mirabile structure in giraffes is an essential feature balancing physiological difficulties arising due to the extensive heart-to-head distance and might fulfill the same function in other long-necked artiodactyls.

Unsustainable hunting, both illegal and legal, has led to the extirpation of many species. In the last 35 years giraffe Giraffa spp. populations have declined precipitously, with extinctions documented in seven African countries. Amongst the various reasons for these population declines, poaching is believed to play an important role in some areas. Giraffes are primarily hunted for consumption and for the use of their body parts as trophies and in traditional medicine. However, the socio-economic factors that correlate with the use of giraffe body parts are not well understood. We conducted our study in Tsavo Conservation Area, Kenya, which experiences high levels of poaching. We used semi-structured surveys amongst 331 households to document how giraffe body parts are typically acquired and their intended use (i.e. trophy, medicinal or consumptive). We then used logistic regression models to assess the correlations between nine socio-economic factors and the use of giraffe body parts. We found that giraffe body parts had mostly consumptive and trophy uses. One-time suppliers, opportunistic access and widely known markets were the most common means of acquiring giraffe body parts. Results from our models showed that three variables (gender: men, occupation: tourism worker, and land ownership) were correlated significantly and positively with the use of giraffe body parts. We describe the complex links between socio-economic factors and the use of giraffe body parts and highlight the importance of implementing mitigation measures adapted to local contexts to combat a challenge that many species of conservation concern are facing. [ABSTRACT FROM AUTHOR]

The image of a giraffe was added to both wheelmade and handmade pottery, faience plaques, temple walls and rock art in the Meroitic period of the Kingdom of Kush (c. 570 BC–AD550), located in modern-day Sudan. However, giraffes do not appear in contemporary royal and elite art and architecture. This article explores the giraffe motif in Kush and compares this to its use earlier in Sudanese history as well as in neighbouring Egypt. The use of this motif on specific media suggests it was important in the symbolic language shared in the Middle Nile Region, but not in the elite canon, meaning it may be a window into understanding oral traditional practices of this time, that perhaps evolved and circulated over a long time period. [ABSTRACT FROM AUTHOR]

Congenital deformities and neoplasia are poorly documented in wildlife, owing to the difficulty of detection in wild populations. Congenital deformities may lead to premature mortality, thus reducing the chances of thorough documentation. Importantly, neoplasia diagnoses depend on either sampling suspicious lesions from living individuals or access to fresh, undisturbed carcasses, which can prove challenging. We describe five cases of suspected congenital cranial deformities (midfacial cleft, wry nose, and brachygnathia inferior) and two possible cases of cranial neoplasia (orbital bone mass and a soft tissue mass) opportunistically observed in wild giraffe (Giraffa spp.) across their range in Africa. Although cases are largely limited to subjective description because physical examination is often not possible, it is critical to document such observations to help identify and track potential health concerns in wild giraffe populations. [ABSTRACT FROM AUTHOR]

The distinctive long neck of the giraffe (Giraffa camelopardalis) entails functional difficulties brought about by the extended distance between the heart and the head. Blood must be circulated over 2 m from the heart to the brain against gravitational force. The natural movement of the head to ground level would result in a large volume of blood moving toward the brain with the force of gravity. Large blood volumes also rush to the brain during bulls' fighting (necking), rendering the giraffe susceptible to possible brain damage. The natural movement of the head from ground level to fully erect would result in blood moving away from the brain with gravitational force. The lack of blood perfusing the brain can cause fainting. The giraffe, however, suffers neither brain damage nor fainting. What adaptations do giraffes have to counteract these challenges? The aim of this study was to investigate the functionality of the rostral epidural rete mirabile situated just beneath the brain and its possible contribution to successful circulation in long-necked giraffes. The unique rostral epidural rete mirabile structure significantly contributes to counteract physiological challenges. Turns and bends characterize this structural arterial meshwork and subsequently an increased artery length through which blood flow must proceed before entrance into the brain, exerting resistance to blood racing to the brain when the head is lowered to the ground. The brain is supplied mainly by the maxillary artery through the carotid rete, with a rudimentary basilar artery not contributing to the brain's blood supply. The resistance to blood flow due to the structure and position of the rostral epidural rete mirabile when the head is in the upright position is counteracted by the unique carotid-vertebral anastomosis allowing immediate cerebral blood supply. The rostral epidural rete mirabile structure in giraffes is an essential feature balancing physiological difficulties arising due to the extensive heart-to-head distance and might fulfill the same function in other long-necked artiodactyls. [ABSTRACT FROM AUTHOR]

When 0.1 Reticulated Giraffe (Giraffa camelopardalis reticulata) began displaying signs of lameness and a persistent swelling of the front left pastern joint, an operant based training program was created for front foot radiographs to diagnose and better manage lameness. Using a protected contact set up, behaviour was shaped using a positive reinforcement procedure. A series of cued behaviors were trained and used to obtain dorsolateral palmar medial oblique front foot radiographs to aid vets in diagnosing the cause of lameness. By training giraffes for foot radiographs, potential causes of lameness could be identified and better managed. Long-term, the results may help zoos identify best practices for managing and preventing lameness in giraffe and subsequently improve welfare. Training animals to participate in their own medical care can improve welfare by giving them a sense of control within the situation. Training for medical procedures helps to reduce the stress imposed on the giraffe and the associated risks compared to alternative methods.

Lumpy skin disease virus (LSDV) has recently undergone rapid spread, now being reported from more than 80 countries, affecting predominantly cattle and to a lesser extent, water buffalo. This poxvirus was previously considered to be highly host-range restricted. However, there is an increasing number of published reports on the detection of the virus from different game animal species. The virus has not only been shown to infect a wide range of game species under experimental conditions, but has also been naturally detected in oryx, giraffe, camels and gazelle. In addition, clinical lumpy skin disease has previously been described in springbok (Antidorcas marsupialis), an African antelope species, in South Africa. This report describes the characterization of lumpy skin disease virus belonging to cluster 1.2, from field samples from springbok, impala (Aepyceros melampus) and a giraffe (Giraffa camelopardalis) in South Africa using PCR, Sanger and whole genome sequencing. Most of these samples were submitted from wild animals in nature reserves or game parks, indicating that the disease is not restricted to captive-bred animals on game farms or zoological gardens. The potential role of wildlife species in the transmission and maintenance of LSDV is further discussed and requires continuing investigation, as the virus and disease may pose a serious threat to endangered species.

Consumers tend to buy meat based on visual physical characteristics, which are affected by the chemical composition of the meat, and there is very little known about the chemical composition of the meat of giraffe. This study therefore aims to broaden the knowledge base on the chemical composition of giraffe meat, rib bone and liver. Eight different muscles from 15 giraffes were analyzed to determine the chemical composition, yielding an average moisture of 77.2 ± 0.09 g/100 g meat, an average protein of 20.8 ± 0.09 g/100 g meat, an average intramuscular fat (IMF) of 1.4 ± 0.03 g/100 g meat and an average ash of 1.1 ± 0.01 g/100 g meat. There was a significant interaction between sex and muscle for the moisture, protein and ash contents, while only muscle had an effect on the fat content. The mineral content of the bone, liver and Longissimus thoracis et lumborum muscle was also analyzed, and bone was found to be a rich source of calcium (highest concentration), whilst the liver had the highest concentration of iron. The chemical composition of the giraffe meat was such that it could be classified as lean meat.

The Masai giraffe (Giraffa camelopardalis tippelskirchi) is the largest-bodied giraffe and the world's tallest terrestrial animal. With its extreme size and height, the giraffe's unique anatomical and physiological adaptations have long been of interest to diverse research fields. Giraffes are also critical to ecosystems of sub-Saharan Africa, with their long neck serving as a conduit to food sources not shared by other herbivores. Although the genome of a Masai giraffe has been sequenced, the assembly was highly fragmented and suboptimal for genome analysis. Herein we report an improved giraffe genome assembly to facilitate evolutionary analysis of the giraffe and other ruminant genomes. Using SOAPdenovo2 and 170 Gbp of Illumina paired-end and mate-pair reads, we generated a 2.6-Gbp male Masai giraffe genome assembly, with a scaffold N50 of 3 Mbp. The incorporation of 114.6 Gbp of Chicago library sequencing data resulted in a HiRise SOAPdenovo + Chicago assembly with an N50 of 48 Mbp and containing 95% of expected genes according to BUSCO analysis. Using the Reference-Assisted Chromosome Assembly tool, we were able to order and orient scaffolds into 42 predicted chromosome fragments (PCFs). Using fluorescence in situ hybridization, we placed 153 cattle bacterial artificial chromosomes onto giraffe metaphase spreads to assess and assign the PCFs on 14 giraffe autosomes and the X chromosome resulting in the final assembly with an N50 of 177.94 Mbp. In this assembly, 21,621 protein-coding genes were identified using both de novo and homology-based predictions. We have produced the first chromosome-scale genome assembly for a Giraffidae species. This assembly provides a valuable resource for the study of artiodactyl evolution and for understanding the molecular basis of the unique adaptive traits of giraffes. In addition, the assembly will provide a powerful resource to assist conservation efforts of Masai giraffe, whose population size has declined by 52% in recent years.

The critically endangered Nubian giraffe (Giraffa camelopardalis camelopardalis) is distributed in small, fragmented populations across East Africa. Safe immobilisation to facilitate animal monitoring, care, and translocation is important for management directed at ensuring long term survival. Due to a high incidence of reported complications, including mortality during giraffe immobilisations, there is a need for developing and refining techniques and sharing information to facilitate widespread application. This retrospective study utilised immobilisation data acquired during wire snare removals from 80 Nubian giraffe induced with intramuscular etorphine hydrochloride. Recorded data included age (adult, subadult), sex, estimated weight, body condition score, induction and reversal drug dosage, induction time, quality of induction, duration of the procedure, time to reversal administration, and snare wound characteristics. There were no statistically significant differences between males and females for induction quality (p > 0.99), induction time (p = 0.72), and procedure time (p = 0.18). No significant differences were noted between adults and subadults for induction quality (p = 0.16) and procedure time (p = 0.35). There was a significant difference in induction time between adults (7.58 ± 0.42 minutes) and subadults (5.65 ± 0.56 minutes) (p < 0.01). On average, adults received 12.4 mg etorphine while subadults received 11.6 mg. Wound severity did not have a significant impact on induction quality. No mortality was observed. Based on these data, etorphine hydrochloride, followed by rapid reversal, was safe for induction of Nubian giraffe presenting for snare removal and should be considered in similar circumstances. [ABSTRACT FROM AUTHOR]

Browsing enrichment may aid in developing species‐specific behaviors for giraffes managed in zoos as a means of improving animal welfare. By nature, giraffes are tree‐feeding animals, including tree bark, but the extent of food other than leaves as a form of browsing enrichment has not been well investigated. Therefore, to investigate the effectiveness of non‐leaf foraging, three giraffes at the Kyoto City Zoo in Japan were observed for 228 h from May 2019 to February 2020. In conjunction with behavioral instantaneous sampling, tree use (landscape tree or enrichment branch) and plant part (leaves, twigs, or barks) were recorded by the 1‐0 sampling method. There was no significant change in the foraging behavior on the leaves of enriched branches, nor was there any significant change in the foraging behavior of the giraffes, except for one animal in the deciduous phase. No significant changes were observed in rumination or other behaviors between the two phases. Although vegetation foraging behavior significantly decreased, except for one animal, dry hay foraging behavior significantly increased in all the animals during the deciduous phase. Some individuals also showed a significant increase in the foraging behavior for non‐leafy parts of the enrichment branches (twigs and bark) during the deciduous phase. This suggests that in some tree species, giraffes forage on the bark and twigs to compensate for the loss of leaves during the deciduous phase, similar to feeding on hay or hay cubes as a substitute for tree leaves. [ABSTRACT FROM AUTHOR]

When 0.1 Reticulated Giraffe (Giraffa camelopardalis reticulata) began displaying signs of lameness and a persistent swelling of the front left pastern joint, an operant based training program was created for front foot radiographs to diagnose and better manage lameness. Using a protected contact set up, behaviour was shaped using a positive reinforcement procedure. A series of cued behaviors were trained and used to obtain dorsolateral palmar medial oblique front foot radiographs to aid vets in diagnosing the cause of lameness. By training giraffes for foot radiographs, potential causes of lameness could be identified and better managed. Long-term, the results may help zoos identify best practices for managing and preventing lameness in giraffe and subsequently improve welfare. Training animals to participate in their own medical care can improve welfare by giving them a sense of control within the situation. Training for medical procedures helps to reduce the stress imposed on the giraffe and the associated risks compared to alternative methods. [ABSTRACT FROM AUTHOR]

Simple Summary: Long-necked animals like giraffes face the challenge of adequately supplying blood to the brain. The heart of such tall animals must work harder to pump blood over a 2 m head-to-heart distance against the force of gravity compared to a short-necked animal. Does a mechanism exist to assist tall animals in overcoming this challenge? We found that there is a possibility of a mechanism that can enhance blood flow to the brain and a subsequent increase in oxygen supply, which will reduce the heart's workload to pump blood over a 2 m distance in a Giraffe. In short-necked animals, the workload on the heart is less to supply blood to the brain adequately; therefore, no additional mechanism is necessary. We, however, suggest that a similar mechanism might have been functional in dinosaurs with extremely long necks to help with adequate blood supply to the brain by comparing relevant giraffe physiology and anatomy with existing data on paleontology. Adult giraffes reach heights of 4.5 m with a heart-to-head distance of over 2 m, making cranial blood supply challenging. Ultrasound confirmed that the giraffe jugular vein collapses during head movement from ground level to fully erect, negating the possibility of a siphon mechanism in the neck. We showed that a short-length siphon structure over a simulated head-to-heart distance for a giraffe significantly influences flow in a collapsible tube. The siphon structure is determined according to brain case measurements. The short-length siphon structure in a shorter-necked ostrich showed no significant increase in flow. The shorter head-to-heart distance might be the reason for the lack of effect in ostriches. A siphon mechanism situated in the cranium is certainly possible, with a significant effect exerted on the amount of pressure the heart must generate to allow adequate cranial blood perfusion in a long-necked giraffe. The study validated that a cranial-bound siphon structure can operate and will be of significant value for adequate cranial blood perfusion in long-necked species such as giraffes and might also have existed in extinct species of long-necked dinosaurs. [ABSTRACT FROM AUTHOR]

Giraffes (Giraffa camelopardalis) are found in zoos all over the world. In recent years, numerous researchers have documented complex sociality in these mammals. They highlighted that giraffes have non-random preferences in their choices of social partners, which can depend on various factors such as age, sex, and kinship. One of the still little-known aspects is how the social structure of giraffes is formed in captivity. Moreover, the scientific literature about some aspects of the social structure of giraffes in captivity (i.e., proximity or affiliative interactions) is scarce. Our hypothesis was that there would be an association between the social network, based on affiliative reciprocal interactions, and physical proximity within a group of six giraffes (5 females and 1 male) living in a zoo. To test this hypothesis, in addition to the ethological observations, we also used a Geographical Information System (GIS) to study the position of the individuals within the daytime installation. Most of the giraffes had a high number of mutual dyadic interactions, which is connected to high group cohesion. Also, each individual actively selected social partners and formed non-random social bonds. Nevertheless, our hypothesis that there would be a social network–physical proximity association, was confirmed for one dyad and partially confirmed for the other two. The results of this study can be useful to increase the knowledge of giraffe sociality and to develop GIS as a new application in zoo studies.

Animal color pattern is a phenotypic trait that may mediate assortative mixing (also known as homophily), whereby similar looking individuals have stronger social associations. Masai giraffe (Giraffa camelopardalis tippelskirchi) coat spot patterns show high variation and some spot traits appear to be heritable. Giraffes also have high visual acuity, which may facilitate intraspecific communication and recognition based on spot patterns. Giraffe groupings are dynamic, merging and splitting throughout the day, but females form long‐term associations. We predicted that adult female giraffes show stronger associations with other females that have similar spot pattern traits. We quantified the spot pattern characteristics of 399 adult female Masai giraffes and determined the pattern similarity among pairs (dyads) in their social network. We then tested for an association between coat pattern similarity (spot size, shape, and orientation) and dyadic association strength, and quantified assortative mixing. The strength of social associations was positively correlated with similarity in spot shape. Our results are compatible with assortativity by coat patterns that are similar between mother and offspring, potentially reflecting an effect of relatedness on both pattern similarity and female social associations. These results offer evidence that spot pattern could function as a visual cue for intraspecific communication and kin or individual recognition in a fission‐fusion species. [ABSTRACT FROM AUTHOR]

While investigating a giraffe's death in a Vietnamese zoo, we successfully identified and isolated Lumpy skin disease virus (LSDV) from skin nodule biopsies and ruptured nodule wound swab samples. Phylogenetic analysis indicated that the isolate obtained in this study was closely related to the previous Vietnamese and Chinese LSDV strains from cattle. This is the first report on the genome detection and isolation of LSDV in a diseased giraffe in Vietnam. Further study is needed to better understand the epidemiology of this disease in wildlife. [ABSTRACT FROM AUTHOR]

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Abstract Background The majority of structural variation in genomes is caused by insertions of transposable elements (TEs). In mammalian genomes, the main TE fraction is made up of autonomous and non-autonomous non-LTR retrotransposons commonly known as LINEs and SINEs (Long and Short Interspersed Nuclear Elements). Here we present one of the first population-level analysis of TE insertions in a non-model organism, the giraffe. Giraffes are ruminant artiodactyls, one of the few mammalian groups with genomes that are colonized by putatively active LINEs of two different clades of non-LTR retrotransposons, namely the LINE1 and RTE/BovB LINEs as well as their associated SINEs. We analyzed TE insertions of both types, and their associated SINEs in three giraffe genome assemblies, as well as across a population level sampling of 48 individuals covering all extant giraffe species. Results The comparative genome screen identified 139,525 recent LINE1 and RTE insertions in the sampled giraffe population. The analysis revealed a drastically reduced RTE activity in giraffes, whereas LINE1 is still actively propagating in the genomes of extant (sub)-species. In concert with the extremely low activity of the giraffe RTE, we also found that RTE-dependent SINEs, namely Bov-tA and Bov-A2, have been virtually immobile in the last 2 million years. Despite the high current activity of the giraffe LINE1, we did not find evidence for the presence of currently active LINE1-dependent SINEs. TE insertion heterozygosity rates differ among the different (sub)-species, likely due to divergent population histories. Conclusions The horizontally transferred RTE/BovB and its derived SINEs appear to be close to inactivation and subsequent extinction in the genomes of extant giraffe species. This is the first time that the decline of a TE family has been meticulously analyzed from a population genetics perspective. Our study shows how detailed information about past and present TE activity can be obtained by analyzing large-scale population-level genomic data sets.

Trichuris spp. are nematode parasites infecting wild ruminants in zoological institutions worldwide. These helminths cause significant morbidity in giraffe (Giraffa camelopardalis) and other hoofstock located in zoological institutions throughout the United States. Historically, studies and institutions have used a variety of nematode detection methods with various flotation solutions. Optimization of Trichuris egg detection is necessary for monitoring collections. Fecal and soil optimized protocols were generated in this study using samples containing Trichuris eggs from multiple semi free-ranging zoological institutions. First, Sheather's sugar (specific gravity (SG) 1.27), sucrose (SG 1.40), magnesium sulfate (SG 1.26), and zinc sulfate (SG 1.18) were compared as flotation solutions by quantitative eggs per gram using a modified Stoll method. Then a soil recovery method was optimized comparing Tween 20, sodium hydroxide, Dawn™ (Procter and Gamble) detergent, and sodium chloride as liberating solutions to free eggs from the soil. We found that Sheather's sugar and sucrose solutions were the most effective for Trichuris egg detection, and either sodium hydroxide or sodium chloride liberated eggs from soil.

Abstract Background Brucellosis is a contagious zoonotic disease of great public health and economic significance especially in developing countries. The disease affects humans and several species of livestock and wildlife. Studies on Brucellosis in wildlife in Uganda have been limited to single populations particularly in Queen Elizabeth National Park. This study aimed at estimating the percentage of positive samples of Brucella spp. in wildlife in four major national parks of Uganda. This was a retrospective survey which utilized archived samples collected from wildlife during the annual disease surveillance activities between 2013 and 2017. Results A total of 241 samples from seven species namely African buffalo (Syncerus caffer, n = 109), African elephant (Loxodonta africana, n = 22), giraffe (Giraffa camelopardalis rothschildi, n = 41), Uganda kob (Kobus kob thomasi, n = 36), lion (Panthera leo, n = 6), plain zebra (Equus quagga, n = 25), and bushbuck (Tragelaphus scriptus, n = 2), were tested for antibodies using the Rose Bengal Plate Test. The overall percentage of positive samples in the four national parks was 31.1% (75/241; 95% CI: 25.6–37.2). Kidepo Valley National Park had a significantly higher percentage of positive samples of 55.9% (19/34; 95% CI: 39.5–71.1) compared to other sampled national parks (p

Patterns of association in animal societies vary through space and time. Understanding such variation is key to predicting inter- and intra-population variation across factors as diverse as gene flow, disease transmission, and resilience to climate change. Here, we use 3.5 years of observational data, coupled with static and dynamic methods in social network analysis, to investigate patterns of social association in wild giraffe in the northern Namib Desert, Namibia (a habitat of extreme aridity for the species). Our static analyses reveal similar robust nested social communities, but less distinct community structures, than those found in giraffe populations inhabiting less arid environments. Furthermore, results of our dynamic social network analyses show increased social connectivity in this population during the morning and hot-dry season. These temporal patterns align with patterns of thermoregulatory and sociosexual behaviour in this population. However, they differ from temporal patterns of connectivity revealed in populations in less arid environments. Combined, results of both our static and temporal analyses suggest that while the major characteristics of giraffe society persist through space and time, there is substantial variation in the strength of patterns of social connectivity both within and between populations at multiple spatiotemporal resolutions. Significance statement: Animal social networks are inherently dynamic. Patterns of connectivity can be shaped by both social factors and habitat variation across space and time. We reveal that a population of uniquely desert-dwelling giraffe maintains a robust, albeit weaker, community structure in a habitat at the extreme edge of its natural range. We also reveal variation in intra-population temporal patterns of connectivity at both daily and seasonal resolutions. Combined, these results suggest that while the major characteristics of giraffe society persist across habitats and through time, the strength of patterns of social connectivity markedly varies in line with habitat factors both within and between populations. [ABSTRACT FROM AUTHOR]

Abstract Objective Skeletal dysplasias, cartilaginous or skeletal disorders that sometimes result in abnormal bone development, are seldom reported in free-ranging wild animals. Here, we use photogrammetry and comparative morphometric analyses to describe cases of abnormal appendicular skeletal proportions of free-ranging giraffe in two geographically distinct taxa: a Nubian giraffe (Giraffa camelopardalis camelopardalis) in Murchison Falls National Park, Uganda and an Angolan giraffe (Giraffa giraffa angolensis) on a private farm in central Namibia. Results These giraffe exhibited extremely shortened radius and metacarpal bones relative to other similarly aged giraffe. Both giraffe survived to at least subadult life stage. This report documents rare occurrences of these apparent skeletal dysplasias in free-ranging wild animals and the first records in giraffe.

1. Dispersal is a critical process that shapes the structure of wild animal populations. In species that form multi-level societies, natal dispersal might be social (associating with a different social community while remaining near the natal area), spatial (moving away from the natal area while continuing to associate with the same community) or both social and spatial (associating with a different community and moving away from the natal area). 2. For such species, classical spatial measures of dispersal, such as distance moved, might not capture social dispersal. 3. We examined dispersal outcomes for 67 male and 70 female giraffe calves over 7 years in a large, unfenced, ecologically heterogeneous landscape. We tested predictions about the influence of sex, food availability, low-and high-impact human settlements, and local giraffe population density on social or spatial dispersal, dispersal distance, and age of dispersal. 4. We found that dispersal is sex-specific, with females being predominately philopatric. When dispersing, both sexes did so at a mean of 4 years of age. Most (69% of total) young males dispersed, with 84% of male dispersers associating with a different adult female social community than that of their mother, but one in four of these dispersers remained spatially near to their natal area. For adolescent males that dispersed socially but not spatially, overlapping female social communities may represent a potential pool of unrelated mating partners without the risks of travelling to unfamiliar areas. Just 26% of young females dispersed and half of these continued to associate with the adult female social community into which they were born, confirming the importance of maintaining ties among females from calf to adulthood. Furthermore, individuals born farther from high-impact human settlements were more likely to spatially or socially-and-spatially disperse, move greater distances from their natal areas, and disperse at a younger age. 5. Our study highlights the potential importance of social structure in dispersal decisions, and of tracking social structure when studying dispersal in multi-level societies, as effective dispersal can be attained without large-scale spatial displacements. [ABSTRACT FROM AUTHOR]

Abstract Background Congenital goiter is a common thyroid metabolic disorder characterized by low levels of thyroid hormone, subsequent secretion of excess thyroid-stimulating hormone (TSH) from the pituitary gland, and compensatory hyperplasia of the glands. The presence of signet ring cells (SRCs) does not provide sufficient evidence for the diagnosis of a thyroid tumor, making histopathological diagnosis challenging. In addition, SRCs can also appear in congenital goiter. Therefore, a comprehensive diagnosis of congenital goiter is warranted based on clinical symptoms, autopsy, histopathology, and laboratory examination. Case presentation A juvenile giraffe at the Ordos Zoo in Ordos presented with symptoms of loss of appetite, serious salivation, and slow growth rate since birth. Its height and weight were significantly lower than those of other giraffes of the same age. The animal ultimately died at 17 months of age. Autopsy revelaed that the thyroids were hard, with an uneven surface and with the presence of many small raised follicles, and dense in cross-section. Other organs were visibly atrophic. Histopathologically, diffuse follicles were irregular in size and shape in the hyperplastic goiter. Some follicles were collapsed due to lack of colloids. The follicles were lined by single or multiple layers of hyperplastic follicular cells (HFCs), some of which were exfoliated in the lumen. The HFCs were either cuboidal with eosinophilic cytoplasm and many red small granules or showed SRC differentiation, with nuclei pressed to one edge of the cell and distorted by cytoplasmic mucin that appeared as a single clear vacuole HFCs and as a foamy, multivesicular cytoplasmic material in others. Scattered necrosis of myocardial cells and hepatocytes, cerebral hemorrhage, necrosis of intestinal villi, and obvious atrophy of organs were also observed. Immunohistochemical tests were strongly positive for thyroglobulin and thyroid transcription factor-1 (TTF-1) in the cytoplasm of HFCs. Conclusions Here we present a case of congenital goiter with areas of SRC differentiation in the thyroid of a juvenile giraffe.

Abstract Swabbing scat has proved to be an effective noninvasive method to collect DNA from mammals in the field. Previously, this method has relied on preservative liquids or freezing to preserve the DNA collected on swabs. In this study, we determine the effectiveness of using silica to simply dry the swab in field as an alternative way to prevent DNA degredation. Four species were included in the study; reticulated giraffe, impala, fringe‐eared oryx, and lion. Swabs were taken at multiple time points for giraffe and impala scat samples, with the lion and oryx sampled opportunistically. Mitochondrial DNA was successfully amplified and sequenced from scat swabs from all species; however, effectiveness varied between species, with 81.8% amplification success rate from swabs taken from impala scat compared to 25% amplification success rate in giraffe. This variation in success rate was overcome by taking multiple swabs, thus increasing the probability of a successful amplification. The true merit of this method is in its simplicity and cheapness; no preservative liquids were required to be brought into the field, at no stage in the 2 weeks of field sampling were samples frozen, and no commercial kits were used for DNA extraction.

Abstract This study presents the first findings on nocturnal behavior patterns of wild Angolan giraffe. We characterized their nocturnal behavior and analyzed the influence of ecological factors such as group size, season, and habitat use. Giraffe were observed using night vision systems and thermal imaging cameras on Okapuka Ranch, Namibia. A total of 77 giraffe were observed during 24 nights over two distinct periods—July–August 2016 (dry season) and February–March 2017 (wet season). Photoperiod had a marked influence on their activity and moving behavior. At dusk, giraffe reduced the time spent moving and increasingly lay down and slept at the onset of darkness. Body postures that likely correspond to rapid eye movement (REM) sleep posture (RSP) were observed 15.8 ± 18.3 min after giraffe sat down. Season had a significant effect with longer RSP phases during the dry season (dry: 155.2 ± 191.1 s, n = 79; wet: 85.8 ± 94.9 s, n = 73). Further analyses of the influence of social behavior patterns did not show an effect of group size on RSP lengths. When a group of giraffe spent time at a specific resting site, several individuals were alert (vigilant) while other group members sat down or took up RSP. Simultaneous RSP events within a group were rarely observed. Resting sites were characterized by single trees or sparse bushes on open areas allowing for good visibility in a relatively sheltered location.

In the wild, giraffes live complex social lives exhibiting fission-fusion social systems. They have sophisticated communication which likely forms a crucial component regulating subgroup dynamics. They spend a large part of their day browsing and traveling over large distances. In captivity, lack of continuous browsing opportunities and limited space can lead to various abnormal and stereotypic behaviours. These stereotypic behaviours can have cascading detrimental health consequences. A behavioural analysis of stereotypic behaviours in giraffes under human care was conducted to evaluate sources of variation within a population and provide management recommendations. The aim of this investigation was threefold: 1. to examine current behaviour of giraffes in Zoological Garden Alipore, Kolkata to advise on their enhanced management; 2. to highlight any behavioural abnormalities and recommend enrichment mechanisms; and 3. to compare the observed stereotypic behaviours with behaviour described in other zoological institutions and in the wild to provide a focal trajectory in the development of guidelines. Four individuals (two adult males, one adult female, and one male calf) were observed outdoors for seven days, three times a day for 30 minutes by instantaneous scan sampling method. During the observation period, the giraffe exhibited oral stereotypy more than any other behaviour recorded, though this was recorded disproportionally between individuals. The giraffe spent a larger amount of time exhibiting oral stereotypy compared to feeding/foraging activities. The study suggests incorporating diet and feeding strategies with provision of natural browse as well as offering enrichment methods to increase the foraging time using various time-engaged feeding devices to mitigate the observed abnormal stereotypic behaviour. Additionally, recommendations are made for expanding the size of the open enclosure to meet guidelines by the Central Zoo Authority, as a minimum.

Background: The majority of structural variation in genomes is caused by insertions of transposable elements (TEs). In mammalian genomes, the main TE fraction is made up of autonomous and non-autonomous non-LTR retrotransposons commonly known as LINEs and SINEs (Long and Short Interspersed Nuclear Elements). Here we present one of the first population-level analysis of TE insertions in a non-model organism, the giraffe. Giraffes are ruminant artiodactyls, one of the few mammalian groups with genomes that are colonized by putatively active LINEs of two different clades of non-LTR retrotransposons, namely the LINE1 and RTE/BovB LINEs as well as their associated SINEs. We analyzed TE insertions of both types, and their associated SINEs in three giraffe genome assemblies, as well as across a population level sampling of 48 individuals covering all extant giraffe species. Results: The comparative genome screen identified 139,525 recent LINE1 and RTE insertions in the sampled giraffe population. The analysis revealed a drastically reduced RTE activity in giraffes, whereas LINE1 is still actively propagating in the genomes of extant (sub)-species. In concert with the extremely low activity of the giraffe RTE, we also found that RTE-dependent SINEs, namely Bov-tA and Bov-A2, have been virtually immobile in the last 2 million years. Despite the high current activity of the giraffe LINE1, we did not find evidence for the presence of currently active LINE1-dependent SINEs. TE insertion heterozygosity rates differ among the different (sub)-species, likely due to divergent population histories. Conclusions: The horizontally transferred RTE/BovB and its derived SINEs appear to be close to inactivation and subsequent extinction in the genomes of extant giraffe species. This is the first time that the decline of a TE family has been meticulously analyzed from a population genetics perspective. Our study shows how detailed information about past and present TE activity can be obtained by analyzing large-scale population-level genomic data sets. [ABSTRACT FROM AUTHOR]

The extinction of 80% of megaherbivore (>1,000 kg) species towards the end of the Pleistocene altered vegetation structure, fire dynamics and nutrient cycling world‐wide. Ecologists have proposed (re)introducing megaherbivores or their ecological analogues to restore lost ecosystem functions and reinforce extant but declining megaherbivore populations. However, the effects of megaherbivores on smaller herbivores are poorly understood.We used long‐term exclusion experiments and multispecies hierarchical models fitted to dung counts to test (a) the effect of megaherbivores (elephant and giraffe) on the occurrence (dung presence) and use intensity (dung pile density) of mesoherbivores (2–1,000 kg), and (b) the extent to which the responses of each mesoherbivore species was predictable based on their traits (diet and shoulder height) and phylogenetic relatedness.Megaherbivores increased the predicted occurrence and use intensity of zebras but reduced the occurrence and use intensity of several other mesoherbivore species. The negative effect of megaherbivores on mesoherbivore occurrence was stronger for shorter species, regardless of diet or relatedness.Megaherbivores substantially reduced the expected total use intensity (i.e. cumulative dung density of all species) of mesoherbivores, but only minimally reduced the expected species richness (i.e. cumulative predicted occurrence probabilities of all species) of mesoherbivores (by <1 species).Simulated extirpation of megaherbivores altered use intensity by mesoherbivores, which should be considered during (re)introductions of megaherbivores or their ecological proxies. Species' traits (in this case shoulder height) may be more reliable predictors of mesoherbivores' responses to megaherbivores than phylogenetic relatedness, and may be useful for predicting responses of data‐limited species. [ABSTRACT FROM AUTHOR]

A 10-y-old giraffe (Giraffa camelopardalis reticulata) bull developed colic after a 3-mo history of reduced feed consumption. Physical examination and management were performed with 2 standing sedations. The giraffe developed metabolic alkalosis and progressive pre-renal azotemia followed by compensatory respiratory acidosis and paradoxical aciduria. A metallic "ping" sound was auscultated on the left side near ribs 10–12. The giraffe was euthanized given the grave prognosis, and postmortem examination confirmed left displacement of the abomasum (LDA) with fluid sequestration (150–190 L [40–50 gal]) within the rumen. Dental disease was evident at postmortem examination and perimortem skull computed tomography. To ensure cases of LDA are not overlooked, the position of the abomasum must be noted during postmortem examination prior to removal of the gastrointestinal tract. The risk factors for the development of LDA in giraffes are not known, and associations such as those of dairy cattle (hypocalcemia, high-concentrate low-fiber diet, and indoor housing) remain to be elucidated. [ABSTRACT FROM AUTHOR]

Adult giraffes reach heights of 4.5 m with a heart-to-head distance of over 2 m, making cranial blood supply challenging. Ultrasound confirmed that the giraffe jugular vein collapses during head movement from ground level to fully erect, negating the possibility of a siphon mechanism in the neck. We showed that a short-length siphon structure over a simulated head-to-heart distance for a giraffe significantly influences flow in a collapsible tube. The siphon structure is determined according to brain case measurements. The short-length siphon structure in a shorter-necked ostrich showed no significant increase in flow. The shorter head-to-heart distance might be the reason for the lack of effect in ostriches. A siphon mechanism situated in the cranium is certainly possible, with a significant effect exerted on the amount of pressure the heart must generate to allow adequate cranial blood perfusion in a long-necked giraffe. The study validated that a cranial-bound siphon structure can operate and will be of significant value for adequate cranial blood perfusion in long-necked species such as giraffes and might also have existed in extinct species of long-necked dinosaurs.

Gut microbiota has been demonstrated to be associated with multiple gastrointestinal diseases, but information regarding the gut microbial alternations in diarrheic giraffe remains scarce. Here, 16S rDNA and ITS gene amplicon sequencing were conducted to investigate the gut microbial composition and variability in diarrheic giraffes. Results demonstrated that Firmicutes and Proteobacteria were the most dominant phyla in the gut bacterial community, whereas Ascomycota and Basidiomycota were observed to be predominant in the gut fungal community regardless of health status. However, the species and relative abundance of preponderant bacterial and fungal genera in healthy and diarrheic giraffes were different. In contrast to the relatively stabilized gut fungal community, gut bacterial community displayed a significant decrease in the alpha diversity, accompanied by distinct changes in taxonomic compositions. Bacterial taxonomic analysis revealed that the relative abundances of eight phyla and 12 genera obviously increased, whereas the relative abundances of two phyla and eight genera dramatically decreased during diarrhea. Moreover, the relative richness of five fungal genera significantly increased, whereas the relative richness of seven fungal genera significantly declined in diarrheic giraffes. Taken together, this study demonstrated that diarrhea could cause significant alternations in the gut microbial composition of giraffes, and the changes in the gut bacterial community were more significant than those in the gut fungal community. Additionally, investigating the gut microbial characteristics of giraffes in different health states is beneficial to provide a theoretical basis for establishing a prevention and treatment system for diarrhea from the gut microbial perspective.

Gut microbiota has been demonstrated to be associated with multiple gastrointestinal diseases, but information regarding the gut microbial alternations in diarrheic giraffe remains scarce. Here, 16S rDNA and ITS gene amplicon sequencing were conducted to investigate the gut microbial composition and variability in diarrheic giraffes. Results demonstrated that Firmicutes and Proteobacteria were the most dominant phyla in the gut bacterial community, whereas Ascomycota and Basidiomycota were observed to be predominant in the gut fungal community regardless of health status. However, the species and relative abundance of preponderant bacterial and fungal genera in healthy and diarrheic giraffes were different. In contrast to the relatively stabilized gut fungal community, gut bacterial community displayed a significant decrease in the alpha diversity, accompanied by distinct changes in taxonomic compositions. Bacterial taxonomic analysis revealed that the relative abundances of eight phyla and 12 genera obviously increased, whereas the relative abundances of two phyla and eight genera dramatically decreased during diarrhea. Moreover, the relative richness of five fungal genera significantly increased, whereas the relative richness of seven fungal genera significantly declined in diarrheic giraffes. Taken together, this study demonstrated that diarrhea could cause significant alternations in the gut microbial composition of giraffes, and the changes in the gut bacterial community were more significant than those in the gut fungal community. Additionally, investigating the gut microbial characteristics of giraffes in different health states is beneficial to provide a theoretical basis for establishing a prevention and treatment system for diarrhea from the gut microbial perspective. [ABSTRACT FROM AUTHOR]

Trichuris spp. are nematode parasites infecting wild ruminants in zoological institutions worldwide. These helminths cause significant morbidity in giraffe (Giraffa camelopardalis) and other hoofstock located in zoological institutions throughout the United States. Historically, studies and institutions have used a variety of nematode detection methods with various flotation solutions. Optimization of Trichuris egg detection is necessary for monitoring collections. Fecal and soil optimized protocols were generated in this study using samples containing Trichuris eggs from multiple semi free-ranging zoological institutions. First, Sheather's sugar (specific gravity (SG) 1.27), sucrose (SG 1.40), magnesium sulfate (SG 1.26), and zinc sulfate (SG 1.18) were compared as flotation solutions by quantitative eggs per gram using a modified Stoll method. Then a soil recovery method was optimized comparing Tween 20, sodium hydroxide, Dawn™ (Procter and Gamble) detergent, and sodium chloride as liberating solutions to free eggs from the soil. We found that Sheather's sugar and sucrose solutions were the most effective for Trichuris egg detection, and either sodium hydroxide or sodium chloride liberated eggs from soil. Trichuris egg seen in fecal flotation of giraffe feces. [Display omitted] • Optimization of giraffe Trichuris egg detection. • Soil Trichuris recovery methods analysis. • Multi-institutional giraffe Trichuris evaluation. [ABSTRACT FROM AUTHOR]

Populations are typically defined as spatially contiguous sets of individuals, but large populations of social species can be composed of discrete social communities that often overlap in space. Masai giraffes (Giraffa camelopardalis tippelskirchi) of Tanzania live in distinct social subpopulations that overlap spatially, enabling us to simultaneously explore environmental and social factors correlated with demographic variation in a metapopulation of >1,400 adult females and calves. We considered statistically distinct communities in the social network as subpopulations and tested for variation among the 10 subpopulations in adult female survival, calf survival, and reproductive rate (calf‐to‐adult female ratio). We then related variation in demographic rates among subpopulations to differences in vegetation, soil type, proximity to 2 types of human settlements, local giraffe population density, and social metrics of relationship strength and exclusivity among adult females. We did not find any among‐subpopulation effects on adult female survival, suggesting adult female survival is buffered against environmental heterogeneity among subpopulations. Variation in calf demographic rates among subpopulations were correlated with vegetation, soils, anthropogenic factors, and giraffe population density but not with adult female relationship metrics, despite substantial spatial overlap. Subpopulations with more dense bushlands in their ranges had lower calf survival probabilities, and those closer to human settlements had higher reproductive rates, possibly because of spatial gradients in natural predation. Reproductive rates were higher in subpopulations with more volcanic soils, and calf survival probabilities were greater in subpopulations with higher local adult female densities, possibly related to higher‐quality habitat associated with fertile soils or lower predation risk, or to greater competitive ability. The variation in fitness among subpopulations suggests that giraffes do not move unhindered among resource patches to equalize reproductive success, as expected according to an ideal free distribution. The differences in calf survival and reproductive rates could rather indicate intercommunity differences in competitive ability, perception, learning, or experience. Our approach of comparing demography among spatially overlapping yet distinct socially defined subpopulations provides a biologically meaningful way to quantify environmental and social factors influencing fine‐scale demographic variation for social species. © 2021 The Wildlife Society. : We compared adult female survival, calf survival, and reproductive rates among spatially overlapping yet socially distinct subpopulations to quantify fine‐scale environmental and social factors that influence demographic variation. Adult female survival was high and constant across subpopulations, but reproductive rates were higher in subpopulations with more volcanic soils and closer to human settlements, and calf survival was greater in subpopulations with higher local adult female densities and with less dense bushlands. [ABSTRACT FROM AUTHOR]

Background: Comparative cognition has historically focused on a few taxa such as primates, birds or rodents. However, a broader perspective is essential to understand how different selective pressures affect cognition in different taxa, as more recently shown in several studies. Here we present the same battery of cognitive tasks to two understudied ungulate species with different socio-ecological characteristics, European bison (Bison bonasus) and forest buffalos (Syncerus caffer nanus), and we compare their performance to previous findings in giraffes (Giraffa camelopardalis). We presented subjects with an Object permanence task, Memory tasks with 30 and 60 s delays, two inference tasks based on acoustic cues (i.e. Acoustic inference tasks) and a control task to check for the use of olfactory cues (i.e. Olfactory task). Results: Overall, giraffes outperformed bison and buffalos, and bison outperformed buffalos (that performed at chance level). All species performed better in the Object permanence task than in the Memory tasks and one of the Acoustic inference tasks (which they likely solved by relying on stimulus enhancement). Giraffes performed better than buffalos in the Shake full Acoustic inference task, but worse than bison and buffalos in the Shake empty Acoustic inference task. Conclusions: In sum, our results are in line with the hypothesis that specific socio-ecological characteristics played a crucial role in the evolution of cognition, and that higher fission-fusion levels and larger dietary breadth are linked to higher cognitive skills. This study shows that ungulates may be an excellent model to test evolutionary hypotheses on the emergence of cognition. [ABSTRACT FROM AUTHOR]

Abstract Population numbers of Kordofan giraffe (Giraffa camelopardalis antiquorum) have declined throughout its range by more than 85% in the last three decades, including in the isolated easternmost population found in the Garamba National Park (NP) in the Democratic Republic of Congo. We provide new data on the conservation status and ecology of Kordofan giraffe in Garamba NP, specifically on the current population dynamics, distribution patterns, and spatial ecology for informed conservation management decisions. Data were gathered between September 26, 2016, and August 17, 2017, through direct observation and from eight GPS satellite collars deployed in early 2016. Movements, distribution patterns, and autocorrelated kernel density home ranges were estimated using the Continuous‐Time Movement Modeling (CTMM) framework. We then compared results with home ranges calculated using the kernel density estimation (95% KDE) method. The Garamba NP population was estimated to be 45 giraffe with a female‐dominated sex ratio (35% males; 65% females), and adult‐dominated age class ratio (11.2% juveniles; 17.7% subadults; 71.1% adults). The giraffe's distribution was limited to the south‐central sector of the Park, and giraffe were divided over different areas with some degree of connectivity. The average giraffe home range size was 934.3 km2 using AKDE and 268.8 km2 using KDE. Both methods have shown surprisingly large home ranges despite of the relatively high humidity of Garamba NP. Based on the outcomes of this research, urgent conservation action is needed to protect Garamba's remaining giraffe population.

Animals constitute a vast share of petroglyphs recorded in Dakhleh Oasis, Egypt, during the past 30 years by the Petroglyph Unit of the Dakhleh Oasis Project. In this paper I provide a detailed overview of all the animal species which can be fairly certainly recognised among the rich zoomorphic corpus of figures, which counts 1429 figures. The animals are discussed mainly in terms of techniques of production, other formal traits, and chronology. Their potential significance is here intentionally left aside. Each category of zoomorphs is considered separately, beginning with the most abundant depictions of giraffes and oryxes. The final discussion, which concludes the paper, situates Dakhleh zoomorphic rock art in a broader context, as well as touches upon issues related to faunal remains. I pose various questions regarding the analysed material, which may have also some relevance for the entire Western Desert of Egypt.