1. Effects of first-generation in utero exposure to diesel engine exhaust on second-generation placental function, fatty acid profiles and foetal metabolism in rabbits: preliminary results
- Author
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Rousseau-Ralliard, Delphine, Valentino, Sarah A, Aubrière, Marie-Christine, Dahirel, Michèle, Lallemand, Marie-Sylvie, Archilla, Catherine, Jouneau, Luc, Fournier, Natalie, Richard, Christophe, Aioun, Josiane, Vitorino Carvalho, Anaïs, Jérôme, Lecardonnel, Slama, Rémy, Duranthon, Véronique, Cassee, Flemming R, Chavatte-Palmer, Pascale, Couturier-Tarrade, Anne, dIRAS RA-1, Sub RIVM, Rousseau-Ralliard, Delphine, Valentino, Sarah A., Fournier, Natalie, Slama, Rémy, Cassee, Flemming R., Chavatte-Palmer, Pascale, Couturier-Tarrade, Anne, Biologie du Développement et Reproduction (BDR), École nationale vétérinaire d'Alfort (ENVA)-Institut National de la Recherche Agronomique (INRA), Université Paris Saclay (COMUE), PremUp Foundation, Institut National de la Santé et de la Recherche Médicale (INSERM)-Sorbonne Université (SU)-Université Paris Descartes - Paris 5 (UPD5)-CHI Créteil-Institut de Recherche pour le Développement (IRD)-Université Paris-Sud - Paris 11 (UP11)-Université Pierre et Marie Curie - Paris 6 (UPMC)-Université Paris Diderot - Paris 7 (UPD7), EA 4041/4529 Lip (Sys)2, UFR de Pharmacie, Université Paris Sud (Paris 11), Laboratoire de Biochimie, UF Cardio-Vasculaire, Hôpital Européen Georges Pompidou [APHP] (HEGP), Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Hôpitaux Universitaires Paris Ouest - Hôpitaux Universitaires Île de France Ouest (HUPO)-Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Hôpitaux Universitaires Paris Ouest - Hôpitaux Universitaires Île de France Ouest (HUPO), Génétique Animale et Biologie Intégrative (GABI), AgroParisTech-Institut National de la Recherche Agronomique (INRA), Inserm, CNRS, Team of Environmental Epidemiology Applied to Reproduction and Respiratory Health, Institut Albert Bonniot, Université Grenoble Alpes (COMUE) (UGA), Centre for Sustainability, Environment and Health, National Institute for Public Health and the Environment [Bilthoven] (RIVM), Institute of Risk Assessment Sciences, Utrecht University [Utrecht], ANR EPAPP, European Project: 311765,EC:FP7:ERC,ERC-2012-StG_20111109,E-DOHAD(2013), dIRAS RA-1, Sub RIVM, Biologie du développement et reproduction (BDR), École nationale vétérinaire d'Alfort (ENVA)-Institut National de la Recherche Agronomique (INRA)-Centre National de la Recherche Scientifique (CNRS), Institut National de la Santé et de la Recherche Médicale (INSERM)-Sorbonne Universités-Université Paris Descartes - Paris 5 (UPD5)-CHI Créteil-Institut de Recherche pour le Développement (IRD)-Université Paris-Sud - Paris 11 (UP11)-Université Pierre et Marie Curie - Paris 6 (UPMC)-Université Paris Diderot - Paris 7 (UPD7), Hôpital Européen Georges Pompidou, Assistance Publique – Hôpitaux de Paris (AP-HP), Institut National de la Recherche Agronomique (INRA)-AgroParisTech, École nationale vétérinaire - Alfort (ENVA)-Institut National de la Recherche Agronomique (INRA), Institut de Recherche pour le Développement (IRD)-Université Paris-Sud - Paris 11 (UP11)-Université Pierre et Marie Curie - Paris 6 (UPMC)-Université Paris Diderot - Paris 7 (UPD7)-CHI Créteil-Université Paris Descartes - Paris 5 (UPD5)-Institut National de la Santé et de la Recherche Médicale (INSERM), and Université Grenoble Alpes [2016-2019] (UGA [2016-2019])
- Subjects
Male ,0301 basic medicine ,[SDV]Life Sciences [q-bio] ,air pollution ,lcsh:Medicine ,chemistry.chemical_compound ,0302 clinical medicine ,Pregnancy ,intergenerational transmission ,gestational exposure ,foeto-placental phenotype ,membrane lipids ,lcsh:Science ,Vehicle Emissions ,2. Zero hunger ,chemistry.chemical_classification ,Air Pollutants ,Inhalation Exposure ,Multidisciplinary ,Fatty Acids ,Proteasome complex ,3. Good health ,medicine.anatomical_structure ,Liver ,chorion ,Maternal Exposure ,In utero ,Prenatal Exposure Delayed Effects ,Female ,Arachidonic acid ,Rabbits ,diesel motor ,Polyunsaturated fatty acid ,placenta ,Offspring ,moteur diésel ,Context (language use) ,Article ,Andrology ,03 medical and health sciences ,Fetus ,Placenta ,Developmental biology ,medicine ,Animals ,micromass ,plasma ,lcsh:R ,Fatty acid ,030104 developmental biology ,chemistry ,13. Climate action ,polluant atmosphérique ,lcsh:Q ,Transcriptome ,030217 neurology & neurosurgery - Abstract
Atmospheric pollution has major health effects on directly exposed subjects but intergenerational consequences are poorly characterized. We previously reported that diesel engine exhaust (DE) could lead to structural changes in the placenta of in utero exposed rabbits (first generation, F1). The effects of maternal exposure to DE were further studied on second-generation (F2) rabbits. Pregnant F0 females were exposed to filtered, diluted DE (1 mg/m3, median particle diameter: 69 nm) or clean filtered air (controls) for 2 h/day, 5 days/week by nose-only exposure during days 3–27 post-conception (dpc). Adult female offspring (F1) were mated to control males: F1 tissues and F2 foeto-placental units were collected at 28 dpc and placental structure and gene expression (microarray) analysed. Fatty acid profiles were determined in foetal and maternal plasma, maternal liver and placenta. In F1, compared to controls, hepatic neutral lipid contents were increased in exposed animals without change in the blood biochemistry. In F2, the placental lipid contents were higher, with higher monounsaturated fatty acids and reduced pro-inflammatory arachidonic acid (AA), without placental structural changes. Conversely, the proportion of anti-inflammatory n-3 polyunsaturated fatty acids in F2 plasma was increased while that of AA was decreased. Gene set enrichment analyses (GSEA) of F2 placenta transcriptomic data identified that the proteasome complex and ubiquitin pathways genes were over-represented and ion channel function and inflammation pathways genes were under-represented in exposed animals. These preliminary results demonstrate that diesel engine exhaust exposure and in utero indirect exposure should be considered as a programming factor within the context of the DOHaD (Developmental Origins of Health and Disease) with a probable intergenerational transmission.
- Published
- 2019