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Context: Butternut squash seed is rich in ethereal extract with high quality long chain polyunsaturated fatty acids. Knowledge gap: It is unknown how inbreeding influences the ethereal extract character in butternut squash seeds. Purpose: The objective was to evaluate the performance of ethereal extract content and its associated seed characters of butternut squash C. moschata genotypes in three generations of inbreeding S0, S1 and S2 and to identify the predominant fatty acids.Methodology: A randomized complete block design was employed in six (6) genotypes, four (4) treatments and five (5) plants per experimental unit, inbreeding depression (ID) was assessed between generations for the variables ethereal extract (EE), seed weight per fruit (SWF), seed unit weight (SUW) and number of seeds per fruit (NSPF); for the identification of the fatty acids present in the EE, the technique of gas chromatography coupled to mass spectrometry (GC/MC) was used. Results and conclusions: In genotypes 129 and 160 when passing from generation S0 to S1, they did not present DE in the EE character and when passing from generation S1 to generation S2, only genotype 129 presented -DE, the other genotypes increased the EE content. Fluctuations between genotypes were presented for the variables SWF, SUW and NSPF in which a differentiating factor between generations was not identified. When analyzing the EE, it was identified that 66.98% belong to monounsaturated and polyunsaturated fatty acids, where 60.15% belong to oleic acid, and the compound squalene, which is scarcely present in oilseeds, was found among the components., Contextualización: La semilla de zapallo es rica en extracto etéreo con alta calidad de ácidos grasos polinsaturados de cadena larga. Vacío de conocimiento: se desconoce, como influye la endocria con respecto al carácter extracto etéreo en semillas de zapallo.  Propósito: El objetivo fue evaluar el comportamiento del contenido de extracto etéreo y sus caracteres asociados a la semilla de genotipos de zapallo C. moschata en tres generaciones de endocría S0, S1 y S2 e identificar los ácidos grasos predominantes. Metodología: Se empleo un diseño de bloques completos al azar en seis (6) genotipos, cuatro (4) tratamientos y cinco (5) plantas por unidad experimental, se valuó la depresión por endocria (DE) entre generaciones para las variables extracto etéreo (EE), peso de semilla por fruto (PSPF), peso unidad de semilla (PUS) y número de semillas por fruto (NSPF); para la identificación de los ácidos grasos presente en el EE se empleó la técnica de cromatografía de gases acoplada a espectrometría de masas (GC/MC). Resultados y conclusiones: En los genotipos 129 y 160 al pasar de la generación S0 a S1, no presentaron DE en el carácter EE y al pasar de la generación S1 a la generación S2, solo el genotipo 129 presentó -DE, los demás genotipos aumentaron el contenido de EE. Se presentaron fluctuaciones entre genotipos para las variables PSPF, PUS y NSPF en las cuales no se identificó un factor diferenciador entre generaciones. Al analizar el EE se identificó que el 66.98% corresponde a ácidos grasos monoinsaturados y poliinsaturados donde el 60.15% corresponde al ácido oleico, y se encontró dentro de los componentes el compuesto escualeno de escasa presencia en semillas oleaginosas.

This study analysed the HPLC of 14 NBR rubbers and found that there are three important points for sustainable compounding. It is critical to have exact knowledge of the mechanisms of the reactions and interactions of chemicals during rubber mixing, vulcanisation, ageing and usage. The extraction and elution behaviour of ingredients (also of small traces) must be known, together with the impurities of ingredients of the compound., This study analysed the HPLC of 14 NBR rubbers and found that there are three important points for sustainable compounding. It is critical to have exact knowledge of the mechanisms of the reactions and interactions of chemicals during rubber mixing, vulcanisation, ageing and usage. The extraction and elution behaviour of ingredients (also of small traces) must be known, together with the impurities of ingredients of the compound.