1. Interval in the replacement of in vitro culture medium affects the integrity and development of equine preantral follicles
- Author
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Andressa Guidugli Lindquist, Marcelo Marcondes Seneda, Camila Bizarro-Silva, I. Búfalo, S. M. Gonzalez, and F. D. Sarapião
- Subjects
Specific time ,equine preantral follicles ,Ovary ,Equine Species ,Biology ,culture in vitro period ,Andrology ,03 medical and health sciences ,0302 clinical medicine ,Follicular phase ,medicine ,horses ,Developmental stage ,lcsh:Veterinary medicine ,030219 obstetrics & reproductive medicine ,General Veterinary ,follicular morphology ,Significant difference ,0402 animal and dairy science ,Histology ,In vitro culture ,04 agricultural and veterinary sciences ,040201 dairy & animal science ,In vitro ,medicine.anatomical_structure ,lcsh:SF600-1100 ,ovary - Abstract
The efficiency of a culture system is related to the elaboration and replacement of a medium with conditions suitable for follicular development. Recent investigations suggested that in vitro culture medium should be replaced after specific time periods in various species. However, the suitable interval for the exchange of in vitro culture medium has not yet been established in equine species. The objective of this investigation was to evaluate the effect of medium exchange intervals of 24 hours (T24) or 48 hours (T48) for in vitro culture of preantral follicles at 2 or 6 days. At the end of the culture period, the fragments were processed using classical histology. Equine preantral follicles were classified according to morphological integrity and developmental stage. Data analysis was performed using Fisher’s test with a significance level of p0.05). Compared to the medium replacement at six days of culture, there was a statistically significant difference for T24 (68.9%, p
- Published
- 2018
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