1. 气相色谱-质谱法测定食用植物油中多种植物甾醇含量.
- Author
-
黄何何
- Abstract
[Objective] A method for simultaneous determination of ẞ-sitosterol, stigmasterol, campesterol and brassicasterol in edible oil was established by gas chromatography-mass (GC-MS). [Method] Sample was saponified by potassium hydroxide-ethanol solution, n-hexane extraction, concentration, derivatization, and then diluted with n-hexane.GC-MS was used to select ion scanning mode (SIM) and external standard method was used for quantification. [Result] There were good linear relationships for ẞ-sitosterol, stigmasterol, brassicasterol in 5-500 µg/mL, and campesterol in 2.5-250.0 µg/mL, respectively. Linear correlation coefficients (R²) were greater than 0.999. Limits of detection were 0.05- 0.10 mg/kg and limits of quantification were 0.1-0.3 mg/kg, respectively. Adding standard recovery rate was 80.5%-107.8%, relative standard deviation (RSD) was 2.3%-9.8%. [Conclusion] This method is easy to operate, efficient, highly sensitive, and has good repeatability, which is suitable for the determination of ẞ-sitosterol, stigmasterol, campesterol and brassicasterol contents in edible vegetable oil samples. [ABSTRACT FROM AUTHOR]
- Published
- 2024
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