1. Isolation and identification of Candida species in patients with orogastric cancer: susceptibility to antifungal drugs, attributes of virulence in vitro and immune response phenotype.
- Author
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de Sousa, Lourimar Viana Nascimento F., Santos, Vera Lúcia, de Souza Monteiro, Andrea, Dias-Souza, Marcus Vinicíus, Marques, Sirlei Garcia, de Faria, Elaine Speziali, de Oliveira Assunção, Elaine Alves, dos Santos, Simone Gonçalves, Zonis, Juan Moises, de Alvarenga, Daniel Gomes, de Holanda, Rodrigo Assunção, de Sousa, Jaqueline Gontijo, dos Santos, Kênia Valßria, de Resende Stoianoff, Maria Aparecida, Assunção, Elaine Alves de Oliveira, Dos Santos, Kênia Valéria, and Stoianoff, Maria Aparecida de Resende
- Subjects
CANDIDA diagnosis ,CANDIDIASIS treatment ,LIVER cancer ,CANCER invasiveness ,GENETICS of disease susceptibility ,GENETICS ,ANTIFUNGAL agents ,CANDIDA ,CYTOKINES ,LARYNGEAL tumors ,MASS spectrometry ,MICROBIAL sensitivity tests ,MOUTH tumors ,OPPORTUNISTIC infections ,ORAL mucosa ,PHENOTYPES ,THRUSH (Mouth disease) ,GASTROINTESTINAL tumors ,CASE-control method ,DISEASE complications ,PHARMACODYNAMICS ,DIAGNOSIS - Abstract
Background: Because of the inherent immunosuppression of cancer patients opportunistic infections by Candida spp, occur frequently. This study aimed to identify Candida species in the oral mucosa of 59 patients with orogastric cancer (OGC) and to analyze the immunological phenotype of these patients.Methods: The yeasts were identified by MALDI-TOF mass spectrometry (MS). For all isolates, we performed phospholipases and proteinases assays, in vitro adherence to buccal epithelial cells (BEC), minimum inhibitory concentration of antifungal drugs and determined the cytokine profile by Cytometric Bead Array flow citometry assay.Results: C. albicans was the most prevalent species in OGC patients (51.6 %) and control group (66.7 %). Candida spp. strains isolated from OGC patients exhibited better adherence to BEC (p = 0.05) than did the control group. Phospholipases production by Candida strains from OGC patients was lower (51.6 %) than in the control group (61.9 %). Proteinases were detected in 41.9 % and 4.8 % of the yeasts from OGC patients and control group, respectively. Significant differences were found in the serum of OGC patients compared to the control group for IL-2, IL-10, TNF-α, IFN-γ and IL-17.Conclusions: The results of this work suggest increased virulence of yeasts isolated from OGC patients and, that this may interfere with the immune phenotype. [ABSTRACT FROM AUTHOR]- Published
- 2016
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