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A panel of 96 DNA samples that reflects the breadth of genetic diversity in popular Russian cattle breeds has been designed. The panel of cattle DNA contains 11 dairy breeds and 6 beef and beef-dairy breeds. The numbers of animals in each breed group vary from 4 to 8. The main criterion for selection of individual animals within each breed is to maximize the total number of unshared haploid genomes according to pedigree data. The resulting panel is equivalent to USDA MARC Beef Cattle Diversity Panel version 2.1 in the power of SNP identification (number of unshared haploid genomes = 186,1, minimum allele frequency required for its detection with 95 % probability – 1,6 %). Analysis of three SNPs shows an insignificant difference between the allele frequencies in Galloway, Hereford, Grey Ukrainian, and Black Pied herds and those in the panel. Thus, the diversity panel may be useful for identification of genetic markers associated with economically important traits in cattle, evaluation of purebred and crossbred animals, and, probably, for tentative estimation of allele frequencies in commercial populations.

A panel of 96 DNA samples (Russian Cattle Genomic Diversity Panel 1.0 or RCGDP 1.0) characterizing the breadth of genetic diversity in popular Russian cattle breeds was designed. The panel contains from four to eight animals from each of 11 dairy and six dairy-meat and meat breeds. The main criterion for inclusion of individual animals in the panel was that they had, according to the pedigree data, the greatest number of unrelated haploid genomes. The resulting panel of 96 animals was estimated to contain 186.1 unshared haploid genomes and was expected to allow a 95% probability of detecting alleles with a frequency greater than 0.016 in the group. This is comparable to the USDA MARC Beef Cattle Diversity Panel version 2.1. Analysis of three SNPs in the Galloway, Hereford, Grey Ukrainian and Black Pied breeds showed no significant differences in the allele frequencies between the animals in the panel and in the herds. Thus, the designed DNA panel may be useful for SNP selection in the genetic analysis of economically important traits, for identification of purebred and hybrid animals, and, probably, for tentative estimation of allele frequencies in the populations. [ABSTRACT FROM AUTHOR]