1. Glucuronide-sulfate Diconjugate as a Novel Metabolite of Glycyrrhetic Acid in Rat Bile
- Author
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Qiaoling Yu, Ning Jing, Guangji Wang, Jing Jing, Xijing Chen, Weichao Ren, Jiping Wang, Andrew K. Davey, Yue Wang, Jing, Jing, Ren, Weichao, Chen, Xijing, Wang, Yue, Qiaoling, Yu, Wang, Guangji, Davey, Andrew Keith, Wang, Jiping, and Jing, Ning
- Subjects
Male ,Spectrometry, Mass, Electrospray Ionization ,Metabolite ,Electrospray ionization ,Anti-Inflammatory Agents ,Pharmaceutical Science ,bile ,Mass spectrometry ,High-performance liquid chromatography ,Rats, Sprague-Dawley ,Pharmaceutical Sciences ,chemistry.chemical_compound ,Glucuronides ,Sulfate conjugate ,glycyrrhetic acid ,Liquid chromatography–mass spectrometry ,Animals ,Bile ,Pharmacology (medical) ,metabolites ,Biotransformation ,Chromatography, High Pressure Liquid ,Pharmacology ,Chromatography ,Molecular Structure ,biology ,Sulfates ,Chemistry ,Pharmacology and Pharmaceutical Sciences not elsewhere classified ,Mass chromatogram ,LC-MS ,Rats ,Injections, Intravenous ,biology.protein ,Glycyrrhetinic Acid ,Glucuronide ,diconjugate - Abstract
To study the metabolites of glycyrrhetic acid (GA) in rat bile, an analytical method was developed to identify GA and its metabolites by liquid chromatography mass spectrometry (LC-MS). Rat bile was collected after i.v. injection of GA. Three major GA-related peaks were detectable in rat bile by high-performance liquid chromatography (HPLC) analysis at 254 nm. LC-MS spectra showed their protonated molecular ions at m/z 727, 647, and 551. Furthermore, the three metabolites were also confirmed to exist in rat bile on LC-MS total ion chromatogram (TIC). Taken together with the susceptible nature to beta-glucuronidase digestion and alkaline conditions, they were identified to be a novel sulfate-glucuronide diconjugate and the known monoglucuronide and sulfate conjugate, respectively.
- Published
- 2008