1. Lymphocyte G-protein-coupled receptor kinase-2 is upregulated in patients with Alzheimer's disease
- Author
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V. Canonico, Emma Sanzari, Guido Iaccarino, Franco Rengo, Massimo Marchese, Dario Leosco, Walter J. Koch, Luca Golino, Francesca Fortunato, Giuseppe Rengo, Carmela Zincarelli, Leosco, Dario, Fortunato, F, Rengo, Giuseppe, Iaccarino, Guido, Sanzari, E, Golino, L, Zincarelli, Carmela, Canonico, Vincenzo, Marchese, M, Koch, Wj, and Rengo, Franco
- Subjects
Male ,medicine.medical_specialty ,G-Protein-Coupled Receptor Kinase 2 ,Lymphocyte ,diagnosis/enzymology ,Messenger ,genetics/metabolism ,Cell Separation ,diagnosis/enzymology/physiopathology ,Receptors, G-Protein-Coupled ,G-Protein-Coupled ,Alzheimer Disease ,Predictive Value of Tests ,Internal medicine ,Aged ,Biological Markers ,metabolism ,Cognition Disorders ,Disease Progression ,Female ,Humans ,Lymphocytes ,enzymology/metabolism ,RNA ,Receptors ,Up-Regulation ,genetics ,beta-Adrenergic Receptor Kinases ,medicine ,RNA, Messenger ,Cognitive decline ,Receptor ,G protein-coupled receptor ,G protein-coupled receptor kinase ,biology ,General Neuroscience ,Beta adrenergic receptor kinase ,medicine.disease ,Endocrinology ,medicine.anatomical_structure ,Immunology ,biology.protein ,Alzheimer's disease ,Signal transduction ,Biomarkers - Abstract
Alterations in signal transduction pathway of G-protein-coupled receptors (GPCRs) have been found in the cerebrocortex and in the peripheral cultured tissues of patients with Alzheimer's disease (AD). The G-protein-coupled receptor kinase-2 (GRK2) plays an important role in regulating the GPCRs signaling: its increased expression is associated with receptor desensitization. The aim of this study was to explore GRK2 levels in peripheral lymphocytes of AD patients and to establish a correlation between lymphocyte protein concentrations and the degree of cognitive impairment. GRK2 mRNA and protein expression were evaluated in the lymphocytes of AD patients with mild or moderate/severe cognitive impairment and in age-matched healthy subjects. Both GRK2 mRNA and protein expression were higher in AD patients lymphocytes compared to controls. Furthermore, lymphocyte GRK2 levels were significantly correlated to the degree of cognitive decline. Our preliminary data suggest that GRK2 is involved in GPCRs coupling dysfunction observed in AD patients. Further studies are needed in order to verify whether the lymphocyte GRK2 might be utilized as a novel biomarker in AD diagnosis and clinical monitoring.
- Published
- 2007