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2. Clinical and humoral response after SARS-CoV-2 breakthrough infection in patients receiving immunosuppressant therapy

Author

Stalman, Eileen W., Wieske, Luuk, Keijser, Jim B.D., van Dam, Koos P.J., Kummer, Laura Y.L., Wilbrink, Maarten F., van Kempen, Zoé L.E., Killestein, Joep, Volkers, Adriaan G., Tas, Sander W., Boekel, Laura, Wolbink, Gerrit J., van der Kooi, Anneke J., Raaphorst, Joost, Löwenberg, Mark, Takkenberg, R. Bart, D’Haens, Geert R.A.M., Spuls, Phyllis I., Bekkenk, Marcel W., Musters, Annelie H., Post, Nicoline F., Bosma, Angela L., Hilhorst, Marc L., Vegting, Yosta, Bemelman, Frederique J., Voskuyl, Alexandre E., Broens, Bo, Parra Sanchez, Agner, van Els, Cécile A.C. M., de Wit, Jelle, Rutgers, Abraham, de Leeuw, Karina, Horváth, Barbara, Verschuuren, Jan J.G.M., Ruiter, Annabel M., van Ouwerkerk, Lotte, van der Woude, Diane, Allaart, Renée C.F., Onno Teng, Y.K., van Paassen, Pieter, Busch, Matthias H., Brusse, Esther, van Doorn, Pieter A., Baars, Adája E., Hijnen, Dirkjan, Schreurs, Corine R.G., van der Pol, W. Ludo, Goedee, H. Stephan, Steenhuis, Maurice, Keijzer, Sofie, Cristianawati, Olvi, Brinke, Anja ten, Verstegen, Niels J.M., Zwinderman, Koos A.H., van Ham, S. Marieke, Rispens, Theo, Welkers, Matthijs R., Jonges, Marcel, Eftimov, Filip, and Kuijpers, Taco W.

Published
2024
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4. Primary SARS-CoV-2 infection in patients with immune-mediated inflammatory diseases: long-term humoral immune responses and effects on disease activity

Author

van Dam, Koos P. J., Volkers, Adriaan G., Wieske, Luuk, Stalman, Eileen W., Kummer, Laura Y. L., van Kempen, Zoé L. E., Killestein, Joep, Tas, Sander W., Boekel, Laura, Wolbink, Gerrit J., van der Kooi, Anneke J., Raaphorst, Joost, Takkenberg, R. Bart, D’Haens, Geert R. A. M., Spuls, Phyllis I., Bekkenk, Marcel W., Musters, Annelie H., Post, Nicoline F., Bosma, Angela L., Hilhorst, Marc L., Vegting, Yosta, Bemelman, Frederike J., Voskuyl, Alexandre E., Broens, Bo, Sanchez, Agner Parra, van Els, Cécile A. C. M., de Wit, Jelle, Rutgers, Abraham, de Leeuw, Karina, Horváth, Barbara, Verschuuren, Jan J. G. M., Ruiter, Annabel M., van Ouwerkerk, Lotte, van der Woude, Diane, Allaart, Renée C. F., Teng, Y. K. Onno, van Paassen, Pieter, Busch, Matthias H., Jallah, Papay B. P., Brusse, Esther, van Doorn, Pieter A., Baars, Adája E., Hijnen, Dirk Jan, Schreurs, Corine R. G., van der Pol, W. Ludo, Goedee, H. Stephan, Steenhuis, Maurice, Keijzer, Sofie, Keijser, Jim B. D., Cristianawati, Olvi, ten Brinke, Anja, Verstegen, Niels J. M., van Ham, S. Marieke, Rispens, Theo, Kuijpers, Taco W., Löwenberg, Mark, and Eftimov, Filip

Published
2023
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5. mRNA-1273 vaccinated inflammatory bowel disease patients receiving TNF inhibitors develop broad and robust SARS-CoV-2-specific CD8+ T cell responses

Author

van Allaart, Renée CF., Baars, Adája E., Bekkenk, Marcel W., Bemelman, Frederike J., Boekel, Laura, Bos, Amélie V., Bosma, Angela L., Broens, Bo, Brusse, Esther, Busch, Matthias H., Cristianawati, Olvi, van Doorn, Pieter A., Elias, George, van Els, Cécile ACM., van Gils, Marit J., Goedee, H Stephan, Hijnen, Dirk Jan, Hilhorst, Marc L., Horváth, Barbara, Jallah, Papay BP., de Jongh, Rivka, Mirfazeli, Elham S., Musters, Annelie H., Keijser, Jim BD., van Kempen, Zoé LE., Killestein, Joep, Kreher, Christine, de Leeuw, Karina, van der Kooi, Anneke J., van Ouwerkerk, Lotte, van Paassen, Pieter, Cabeza, Virginia Palomares, Parra Sanchez, Agner R., Ludo van der Pol, W., Post, Nicoline F., Raaphorst, Joop, Ruiter, Annabel M., Rutgers, Abraham, Schreurs, Corine RG., Spuls, Phyllis I., Takkenberg, R Bart, Tas, Sander W., Teng, YK Onno, Vegting, Yosta, Verschuuren, Jan JGM., Voskuyl, Alexandre E., de Wit, Jelle, Wolbink, Gerrit J., van der Woude, Diane, Zwinderman, Koos AH., van den Dijssel, Jet, Duurland, Mariël C., Konijn, Veronique AL., Kummer, Laura YL., Hagen, Ruth R., Kuijper, Lisan H., Wieske, Luuk, van Dam, Koos PJ., Stalman, Eileen W., Steenhuis, Maurice, Geerdes, Dionne M., Mok, Juk Yee, Kragten, Angela HM., Menage, Charlotte, Koets, Lianne, Veldhuisen, Barbera, Verstegen, Niels JM., van der Schoot, C Ellen, van Esch, Wim JE., D'Haens, Geert RAM., Löwenberg, Mark, Volkers, Adriaan G., Rispens, Theo, Kuijpers, Taco W., Eftimov, Filip, van Gisbergen, Klaas PJM., van Ham, S Marieke, ten Brinke, Anja, and van de Sandt, Carolien E.

Published
2024
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6. Humoral and cellular immunogenicity, effectiveness and safety of COVID-19 mRNA vaccination in patients with pediatric rheumatic diseases: A prospective cohort study

Author

Hamad Saied, Mohamad, van Straalen, Joeri W., de Roock, Sytze, Verduyn Lunel, Frans M., de Wit, Jelle, de Rond, Lia G.H., Van Nieuwenhove, Erika, Vastert, Bas J., van Montfrans, Joris M., van Royen-Kerkhof, Annet, de Joode-Smink, Gerrie C.J., Swart, Joost F., Wulffraat, Nico M., and Jansen, Marc H.A.

Published
2024
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8. Distinct T cell responsiveness to different COVID-19 vaccines and cross-reactivity to SARS-CoV-2 variants with age and CMV status

Author

Brummelman, Jolanda, primary, Suárez-Hernández, Sara, additional, de Rond, Lia, additional, Bogaard-van Maurik, Marjan, additional, Molenaar, Petra, additional, van Wijlen, Emma, additional, Oomen, Debbie, additional, Beckers, Lisa, additional, Rots, Nynke Y., additional, van Beek, Josine, additional, Nicolaie, Mioara A., additional, van Els, Cécile A. C. M., additional, Boer, Mardi C., additional, Kaaijk, Patricia, additional, Buisman, Anne-Marie, additional, and de Wit, Jelle, additional

Published
2024
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9. Breakthrough SARS-CoV-2 infections with the delta (B.1.617.2) variant in vaccinated patients with immune-mediated inflammatory diseases using immunosuppressants: a substudy of two prospective cohort studies

Author

de Jongh, Rivka, van de Sandt, Carolien, Kuijper, Lisan, Duurland, Mariel, Hagen, Ruth, van den Dijssel, Jet, Kreher, Christine, Bos, Amelie, Palomares Cabeza, Viriginia, Konijn, Veronique, Elias, George, Vallejo, Juan, van Gils, Marrit, Ashhurst, Tom, Nejentsev, Sergey, Mirfazeli, Elham, Boekel, Laura, Stalman, Eileen W, Wieske, Luuk, Hooijberg, Femke, van Dam, Koos P J, Besten, Yaëlle R, Kummer, Laura Y L, Steenhuis, Maurice, van Kempen, Zoé L E, Killestein, Joep, Volkers, Adriaan G, Tas, Sander W, van der Kooi, Anneke J, Raaphorst, Joost, Löwenberg, Mark, Takkenberg, R Bart, D'Haens, Geert R A M, Spuls, Phyllis I, Bekkenk, Marcel W, Musters, Annelie H, Post, Nicoline F, Bosma, Angela L, Hilhorst, Marc L, Vegting, Yosta, Bemelman, Frederike J, Voskuyl, Alexandre E, Broens, Bo, Parra Sanchez, Agner, van Els, Cécile A C M, de Wit, Jelle, Rutgers, Abraham, de Leeuw, Karina, Horváth, Barbara, Verschuuren, Jan J G M, Ruiter, Annabel M, van Ouwerkerk, Lotte, van der Woude, Diane, Allaart, Cornelia F, Teng, Y K Onno, van Paassen, Pieter, Busch, Matthias H, Jallah, Papay B P, Brusse, Esther, van Doorn, Pieter A, Baars, Adája E, Hijnen, Dirk Jan, Schreurs, Corine R G, van der Pol, W Ludo, Goedee, H Stephan, Vogelzang, Erik H, Leeuw, Maureen, Atiqi, Sadaf, van Vollenhoven, Ronald, Gerritsen, Martijn, van der Horst-Bruinsma, Irene E, Lems, Willem F, Nurmohamed, Mike T, Boers, Maarten, Keijzer, Sofie, Keijser, Jim, Boogaard, Arend, Cristianawati, Olvi, ten Brinke, Anja, Verstegen, Niels J M, Zwinderman, Koos A H, van Ham, S Marieke, Rispens, Theo, Kuijpers, Taco W, Wolbink, Gertjan, and Eftimov, Filip

Published
2022
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10. Humoral responses after second and third SARS-CoV-2 vaccination in patients with immune-mediated inflammatory disorders on immunosuppressants: a cohort study

Author

de Jongh, R., van de Sandt, C.E., Kuijper, L., Duurland, M., Hagen, R.R., van den Dijssel, J., Kreher, C., Bos, A., Palomares Cabeza, V., Konijn, V.A.L., Elias, G., Vallejo, J.G., van Gils, M.J., Ashhurst, T.M., Nejentsev, S., Mirfazeli, E.S., Wieske, Luuk, van Dam, Koos P J, Steenhuis, Maurice, Stalman, Eileen W, Kummer, Laura Y L, van Kempen, Zoé L E, Killestein, Joep, Volkers, Adriaan G, Tas, Sander W, Boekel, Laura, Wolbink, Gerrit J, van der Kooi, Anneke J, Raaphorst, Joost, Löwenberg, Mark, Takkenberg, R Bart, D'Haens, Geert R A M, Spuls, Phyllis I, Bekkenk, Marcel W, Musters, Annelie H, Post, Nicoline F, Bosma, Angela L, Hilhorst, Marc L, Vegting, Yosta, Bemelman, Frederike J, Voskuyl, Alexandre E, Broens, Bo, Sanchez, Agner Parra, van Els, Cécile A C M, de Wit, Jelle, Rutgers, Abraham, de Leeuw, Karina, Horváth, Barbara, Verschuuren, Jan J G M, Ruiter, Annabel M, van Ouwerkerk, Lotte, van der Woude, Diane, Allaart, Renée C F, Teng, Y K Onno, van Paassen, Pieter, Busch, Matthias H, Jallah, Papay B P, Brusse, Esther, van Doorn, Pieter A, Baars, Adája E, Hijnen, Dirk Jan, Schreurs, Corine R G, van der Pol, W Ludo, Goedee, H Stephan, Keijzer, Sofie, Keijser, Jim B D, Boogaard, Arend, Cristianawati, Olvi, ten Brinke, Anja, Verstegen, Niels J M, Zwinderman, Koos A H, van Ham, S Marieke, Kuijpers, Taco W, Rispens, Theo, and Eftimov, Filip

Published
2022
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11. Humoral and cellular immunogenicity, effectiveness and safety of COVID-19 mRNA vaccination in patients with pediatric rheumatic diseases: A prospective cohort study

Author

Immuno/reuma onderzoek 7 (Swart), Infection & Immunity, Immuno/reuma onderzoek 1 (Vastert), Child Health, MMB Medische Staf, Immuno/reuma patientenzorg, CTI Van Loosdregt, Immunologie/Reumatologie, Cluster B, Geneeskunde, Immuno/reuma ERN, Hamad Saied, Mohamad, van Straalen, Joeri W, de Roock, Sytze, Verduyn Lunel, Frans M, de Wit, Jelle, de Rond, Lia G H, Van Nieuwenhove, Erika, Vastert, Bas J, van Montfrans, Joris M, van Royen-Kerkhof, Annet, de Joode-Smink, Gerrie C J, Swart, Joost F, Wulffraat, Nico M, Jansen, Marc H A, Immuno/reuma onderzoek 7 (Swart), Infection & Immunity, Immuno/reuma onderzoek 1 (Vastert), Child Health, MMB Medische Staf, Immuno/reuma patientenzorg, CTI Van Loosdregt, Immunologie/Reumatologie, Cluster B, Geneeskunde, Immuno/reuma ERN, Hamad Saied, Mohamad, van Straalen, Joeri W, de Roock, Sytze, Verduyn Lunel, Frans M, de Wit, Jelle, de Rond, Lia G H, Van Nieuwenhove, Erika, Vastert, Bas J, van Montfrans, Joris M, van Royen-Kerkhof, Annet, de Joode-Smink, Gerrie C J, Swart, Joost F, Wulffraat, Nico M, and Jansen, Marc H A

Published
2024

12. Contribution of SARS-CoV-2 infection preceding COVID-19 mRNA vaccination to generation of cellular and humoral immune responses in children

Author

Verheul, Marije K., primary, Vos, Martijn, additional, de Rond, Lia, additional, De Zeeuw-Brouwer, Mary-Lène, additional, Nijhof, Kim H., additional, Smit, Debbie, additional, Oomen, Debbie, additional, Molenaar, Petra, additional, Bogaard, Marjan, additional, van Bergen, Rianne, additional, Middelhof, Irene, additional, Beckers, Lisa, additional, Wijmenga-Monsuur, Alienke J., additional, Buisman, Anne-Marie, additional, Boer, Mardi C., additional, van Binnendijk, Rob, additional, de Wit, Jelle, additional, and Guichelaar, Teun, additional

Published
2023
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15. Contribution of SARS-CoV-2 infection preceding COVID-19 mRNA vaccination to generation of cellular and humoral immune responses in children.

Author

Verheul, Marije K., Vos, Martijn, de Rond, Lia, De Zeeuw-Brouwer, Mary-Lène, Nijhof, Kim H., Smit, Debbie, Oomen, Debbie, Molenaar, Petra, Bogaard, Marjan, van Bergen, Rianne, Middelhof, Irene, Beckers, Lisa, Wijmenga-Monsuur, Alienke J., Buisman, Anne-Marie, Boer, Mardi C., van Binnendijk, Rob, de Wit, Jelle, and Guichelaar, Teun

Subjects
HUMORAL immunity, COVID-19 vaccines, SARS-CoV-2, VACCINATION of children, T cells, COVID-19 pandemic
Abstract

Primary COVID-19 vaccination for children, 5-17 years of age, was offered in the Netherlands at a time when a substantial part of this population had already experienced a SARS-CoV-2 infection. While vaccination has been shown effective, underlying immune responses have not been extensively studied. We studied immune responsiveness to one and/or two doses of primary BNT162b2 mRNA vaccination and compared the humoral and cellular immune response in children with and without a preceding infection. Antibodies targeting the original SARS-CoV-2 Spike or Omicron Spike were measured by multiplex immunoassay. B-cell and Tcell responses were investigated using enzyme-linked immunosorbent spot (ELISpot) assays. The activation of CD4+ and CD8+ T cells was studied by flowcytometry. Primary vaccination induced both a humoral and cellular adaptive response in naive children. These responses were stronger in those with a history of infection prior to vaccination. A second vaccine dose did not further boost antibody levels in those who previously experienced an infection. Infection-induced responsiveness prior to vaccination was mainly detected in CD8+ T cells, while vaccineinduced T-cell responses were mostly by CD4+ T cells. Thus, SARS-CoV-2 infection prior to vaccination enhances adaptive cellular and humoral immune responses to primary COVID-19 vaccination in children. As most children are now expected to contract infection before the age of five, the impact of infection-induced immunity in children is of high relevance. Therefore, considering natural infection as a priming immunogen that enhances subsequent vaccine-responsiveness may help decision-making on the number and timing of vaccine doses. [ABSTRACT FROM AUTHOR]

Published
2024
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16. Disease activity in patients with immune-mediated inflammatory diseases after SARS-CoV-2 vaccinations

Author

van Dam, Koos P.J., primary, Wieske, Luuk, additional, Stalman, Eileen W., additional, Kummer, Laura Y.L., additional, Roosen, Jesse, additional, van Kempen, Zoé L.E., additional, Killestein, Joep, additional, Volkers, Adriaan G., additional, Boekel, Laura, additional, Wolbink, Gerrit J., additional, van der Kooi, Anneke J., additional, Raaphorst, Joost, additional, Löwenberg, Mark, additional, Takkenberg, R. Bart, additional, D'Haens, Geert R.A.M., additional, Spuls, Phyllis I., additional, Bekkenk, Marcel W., additional, Musters, Annelie H., additional, Post, Nicoline F., additional, Bosma, Angela L., additional, Hilhorst, Marc L., additional, Vegting, Yosta, additional, Bemelman, Frederike J., additional, Voskuyl, Alexandre E., additional, Broens, Bo, additional, Sanchez, Agner Parra, additional, van Els, Cécile A.C.M., additional, de Wit, Jelle, additional, Rutgers, Abraham, additional, de Leeuw, Karina, additional, Horváth, Barbara, additional, Verschuuren, Jan J.G.M., additional, Ruiter, Annabel M., additional, van Ouwerkerk, Lotte, additional, van der Woude, Diane, additional, Allaart, Renée C.F., additional, Teng, Y.K. Onno, additional, van Paassen, Pieter, additional, Busch, Matthias H., additional, Jallah, Papay B.P., additional, Brusse, Esther, additional, van Doorn, Pieter A., additional, Baars, Adája E., additional, Hijnen, Dirk Jan, additional, Schreurs, Corine R.G., additional, van der Pol, W.Ludo, additional, Goedee, H. Stephan, additional, Steenhuis, Maurice, additional, Keijzer, Sofie, additional, Keijser, Jim B.D., additional, Cristianawati, Olvi, additional, Rispens, Theo, additional, Brinke, Anja ten, additional, Verstegen, Niels J.M., additional, Marieke van Ham, S., additional, Tas, Sander W., additional, Kuijpers, Taco W., additional, and Eftimov, Filip, additional

Published
2023
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17. Primary SARS-CoV-2 infection in patients with immune-mediated inflammatory diseases:long-term humoral immune responses and effects on disease activity

Author

van Dam, Koos P.J., Volkers, Adriaan G., Wieske, Luuk, Stalman, Eileen W., Kummer, Laura Y.L., van Kempen, Zoé L.E., Killestein, Joep, Tas, Sander W., Boekel, Laura, Wolbink, Gerrit J., van der Kooi, Anneke J., Raaphorst, Joost, Takkenberg, R. Bart, D’Haens, Geert R.A.M., Spuls, Phyllis I., Bekkenk, Marcel W., Musters, Annelie H., Post, Nicoline F., Bosma, Angela L., Hilhorst, Marc L., Vegting, Yosta, Bemelman, Frederike J., Voskuyl, Alexandre E., Broens, Bo, Sanchez, Agner Parra, van Els, Cécile A.C.M., de Wit, Jelle, Rutgers, Abraham, de Leeuw, Karina, Horváth, Barbara, Verschuuren, Jan J.G.M., Ruiter, Annabel M., van Ouwerkerk, Lotte, van der Woude, Diane, Allaart, Renée C.F., Teng, Y. K.Onno, van Paassen, Pieter, Busch, Matthias H., Jallah, Papay B.P., Brusse, Esther, van Doorn, Pieter A., Baars, Adája E., Hijnen, Dirk Jan, Schreurs, Corine R.G., van der Pol, W. Ludo, Goedee, H. Stephan, Steenhuis, Maurice, Keijzer, Sofie, Keijser, Jim B.D., Cristianawati, Olvi, Eftimov, Filip, van Dam, Koos P.J., Volkers, Adriaan G., Wieske, Luuk, Stalman, Eileen W., Kummer, Laura Y.L., van Kempen, Zoé L.E., Killestein, Joep, Tas, Sander W., Boekel, Laura, Wolbink, Gerrit J., van der Kooi, Anneke J., Raaphorst, Joost, Takkenberg, R. Bart, D’Haens, Geert R.A.M., Spuls, Phyllis I., Bekkenk, Marcel W., Musters, Annelie H., Post, Nicoline F., Bosma, Angela L., Hilhorst, Marc L., Vegting, Yosta, Bemelman, Frederike J., Voskuyl, Alexandre E., Broens, Bo, Sanchez, Agner Parra, van Els, Cécile A.C.M., de Wit, Jelle, Rutgers, Abraham, de Leeuw, Karina, Horváth, Barbara, Verschuuren, Jan J.G.M., Ruiter, Annabel M., van Ouwerkerk, Lotte, van der Woude, Diane, Allaart, Renée C.F., Teng, Y. K.Onno, van Paassen, Pieter, Busch, Matthias H., Jallah, Papay B.P., Brusse, Esther, van Doorn, Pieter A., Baars, Adája E., Hijnen, Dirk Jan, Schreurs, Corine R.G., van der Pol, W. Ludo, Goedee, H. Stephan, Steenhuis, Maurice, Keijzer, Sofie, Keijser, Jim B.D., Cristianawati, Olvi, and Eftimov, Filip

Abstract

Background: Patients with immune-mediated inflammatory diseases (IMIDs) on immunosuppressants (ISPs) may have impaired long-term humoral immune responses and increased disease activity after SARS-CoV-2 infection. We aimed to investigate long-term humoral immune responses against SARS-CoV-2 and increased disease activity after a primary SARS-CoV-2 infection in unvaccinated IMID patients on ISPs. Methods: IMID patients on active treatment with ISPs and controls (i.e. IMID patients not on ISP and healthy controls) with a confirmed SARS-CoV-2 infection before first vaccination were included from an ongoing prospective cohort study (T2B! study). Clinical data on infections and increased disease activity were registered using electronic surveys and health records. A serum sample was collected before first vaccination to measure SARS-CoV-2 anti-receptor-binding domain (RBD) antibodies. Results: In total, 193 IMID patients on ISP and 113 controls were included. Serum samples from 185 participants were available, with a median time of 173 days between infection and sample collection. The rate of seropositive IMID patients on ISPs was 78% compared to 100% in controls (p < 0.001). Seropositivity rates were lowest in patients on anti-CD20 (40.0%) and anti-tumor necrosis factor (TNF) agents (60.5%), as compared to other ISPs (p < 0.001 and p < 0.001, respectively). Increased disease activity after infection was reported by 68 of 260 patients (26.2%; 95% CI 21.2–31.8%), leading to ISP intensification in 6 out of these 68 patients (8.8%). Conclusion: IMID patients using ISPs showed reduced long-term humoral immune responses after primary SARS-CoV-2 infection, which was mainly attributed to treatment with anti-CD20 and anti-TNF agents. Increased disease activity after SARS-CoV-2 infection was reported commonly, but was mostly mild. Trial registration: NL74974.018.20, Trial ID: NL8900. Register

Published
2023

19. Primary SARS-CoV-2 infection in patients with immune-mediated inflammatory diseases: long-term humoral immune responses and effects on disease activity

Author

Dam, Koos van, primary, Volkers, Adriaan, additional, Wieske, Luuk, additional, Stalman, Eileen, additional, Kummer, Laura, additional, kempen, Zoe van, additional, Killestein, Joep, additional, Tas, Sander, additional, Boekel, Laura, additional, Wolbink, Gertjan, additional, Kooi, Anneke van der, additional, Raaphorst, Joost, additional, Takkenberg, Bart, additional, D'Haens, Geert, additional, Spuls, Phyllis, additional, Bekkenk, Marcel, additional, Musters, Annelie, additional, Post, Nicoline, additional, Bosma, Angela, additional, Hilhorst, Marc, additional, Vegting, Yosta, additional, Bemelman, Frederike, additional, Voskuyl, Alexandre, additional, Broens, Bo, additional, Sanchez, Agner Parra, additional, Els, Cecile van, additional, de Wit, Jelle, additional, Rutgers, Abraham, additional, de Leeuw, Karina, additional, Horvath, Barbara, additional, Verschuuren, Jan, additional, Ruiter, Annabel, additional, Ouwerkerk, Lotte van, additional, Woude, Diane van der, additional, Allaart, Renee, additional, Teng, Onno, additional, Paassen, Pieter van, additional, Busch, Matthias, additional, Jallah, Papay, additional, Brusse, Esther, additional, Doorn, Pieter van, additional, Baars, Adaja, additional, Hijnen, DirkJan, additional, Schreurs, Corine, additional, Pol, Ludo van der, additional, Goedee, Stephan, additional, Steenhuis, Maurice, additional, Keijzer, Sofie, additional, Keijser, Jim, additional, Cristianawati, Olvi, additional, Brinke, Anja ten, additional, Verstegen, Niels, additional, Ham, Marieke van, additional, Rispens, Theo, additional, Kuijpers, Taco, additional, Lowenberg, Mark, additional, and Eftimov, Filip, additional

Published
2023
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20. Longitudinal Dynamics of the SARS-CoV-2 Antibody Repertoire after SARS-CoV-2 Delta and Omicron Breakthrough Infections in Patients with Immune-Mediated Inflammatory Diseases

Author

Stalman, Eileen, primary, Wieske, Luuk, additional, Keijser, Jim, additional, van Dam, Koos, additional, Kummer, Laura, additional, Wilbrink, Maarten F., additional, van Kempen, Zoé, additional, Killestein, Joep, additional, Volkers, Adriaan G., additional, Tas, Sander, additional, Boekel, Laura, additional, Wolbink, Gertjan, additional, van der Kooi, Anneke J., additional, Raaphorst, Joost, additional, Löwenberg, Mark, additional, Takkenberg, R. Bart, additional, D&apos;Haens, Geert R.A.M., additional, Spuls, Phyllis I., additional, Bekkenk, Marcel W., additional, Musters, Annelie H., additional, Post, Nicoline F., additional, Bosma, Angela L., additional, Hilhorst, Marc L., additional, Vegting, Yosta, additional, Bemelman, Frederike J., additional, Voskuyl, Alexandre, additional, Broens, Bo, additional, Sanchez, Agner Parra, additional, van Els, Cecile, additional, de Wit, Jelle, additional, Rutgers, Abraham, additional, de Leeuw, Karina, additional, Horváth, Barbara, additional, Verschuuren, Jan J.G.M., additional, Ruiter, Annabel M., additional, van Ouwerkerk, Lotte, additional, van der Woude, Diane, additional, Allaart, Cornelia F., additional, Teng, Onno YK, additional, van Paassen, Pieter, additional, Busch, Matthias, additional, Brusse, Esther, additional, van Doorn, Pieter, additional, Baars, Adája E., additional, Hijnen, Dirk Jan, additional, Schreurs, Corine R.G., additional, van der Pol, Ludo, additional, Goedee, H. Stephan, additional, Steenhuis, Maurice, additional, Keijzer, Sofie, additional, Cristianawati, Olvi, additional, ten Brinke, Anja, additional, Verstegen, Niels, additional, Zwinderman, Aeilko H., additional, van Ham, Marieke, additional, Rispens, Theo, additional, Welkers, Matthijs R.A., additional, Jonges, Marcel, additional, Eftimov, Filip, additional, and Kuijpers, Taco, additional

Published
2023
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23. Additional file 1 of Primary SARS-CoV-2 infection in patients with immune-mediated inflammatory diseases: long-term humoral immune responses and effects on disease activity

Author

van Dam, Koos P. J., Volkers, Adriaan G., Wieske, Luuk, Stalman, Eileen W., Kummer, Laura Y. L., van Kempen, Zoé L. E., Killestein, Joep, Tas, Sander W., Boekel, Laura, Wolbink, Gerrit J., van der Kooi, Anneke J., Raaphorst, Joost, Takkenberg, R. Bart, D’Haens, Geert R. A. M., Spuls, Phyllis I., Bekkenk, Marcel W., Musters, Annelie H., Post, Nicoline F., Bosma, Angela L., Hilhorst, Marc L., Vegting, Yosta, Bemelman, Frederike J., Voskuyl, Alexandre E., Broens, Bo, Sanchez, Agner Parra, van Els, Cécile A. C. M., de Wit, Jelle, Rutgers, Abraham, de Leeuw, Karina, Horváth, Barbara, Verschuuren, Jan J. G. M., Ruiter, Annabel M., van Ouwerkerk, Lotte, van der Woude, Diane, Allaart, Renée C. F., Teng, Y. K. Onno, van Paassen, Pieter, Busch, Matthias H., Jallah, Papay B. P., Brusse, Esther, van Doorn, Pieter A., Baars, Adája E., Hijnen, Dirk Jan, Schreurs, Corine R. G., van der Pol, W. Ludo, Goedee, H. Stephan, Steenhuis, Maurice, Keijzer, Sofie, Keijser, Jim B. D., Cristianawati, Olvi, ten Brinke, Anja, Verstegen, Niels J. M., van Ham, S. Marieke, Rispens, Theo, Kuijpers, Taco W., Löwenberg, Mark, and Eftimov, Filip

Abstract

Additional file 1.

Published
2023
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27. Breakthrough SARS-CoV-2 infections with the delta (B.1.617.2) variant in vaccinated patients with immune-mediated inflammatory diseases using immunosuppressants: a substudy of two prospective cohort studies

Author

Boekel, Laura, primary, Stalman, Eileen W, additional, Wieske, Luuk, additional, Hooijberg, Femke, additional, van Dam, Koos P J, additional, Besten, Yaëlle R, additional, Kummer, Laura Y L, additional, Steenhuis, Maurice, additional, van Kempen, Zoé L E, additional, Killestein, Joep, additional, Volkers, Adriaan G, additional, Tas, Sander W, additional, van der Kooi, Anneke J, additional, Raaphorst, Joost, additional, Löwenberg, Mark, additional, Takkenberg, R Bart, additional, D'Haens, Geert R A M, additional, Spuls, Phyllis I, additional, Bekkenk, Marcel W, additional, Musters, Annelie H, additional, Post, Nicoline F, additional, Bosma, Angela L, additional, Hilhorst, Marc L, additional, Vegting, Yosta, additional, Bemelman, Frederike J, additional, Voskuyl, Alexandre E, additional, Broens, Bo, additional, Parra Sanchez, Agner, additional, van Els, Cécile A C M, additional, de Wit, Jelle, additional, Rutgers, Abraham, additional, de Leeuw, Karina, additional, Horváth, Barbara, additional, Verschuuren, Jan J G M, additional, Ruiter, Annabel M, additional, van Ouwerkerk, Lotte, additional, van der Woude, Diane, additional, Allaart, Cornelia F, additional, Teng, Y K Onno, additional, van Paassen, Pieter, additional, Busch, Matthias H, additional, Jallah, Papay B P, additional, Brusse, Esther, additional, van Doorn, Pieter A, additional, Baars, Adája E, additional, Hijnen, Dirk Jan, additional, Schreurs, Corine R G, additional, van der Pol, W Ludo, additional, Goedee, H Stephan, additional, Vogelzang, Erik H, additional, Leeuw, Maureen, additional, Atiqi, Sadaf, additional, van Vollenhoven, Ronald, additional, Gerritsen, Martijn, additional, van der Horst-Bruinsma, Irene E, additional, Lems, Willem F, additional, Nurmohamed, Mike T, additional, Boers, Maarten, additional, Keijzer, Sofie, additional, Keijser, Jim, additional, van de Sandt, Carolien, additional, Boogaard, Arend, additional, Cristianawati, Olvi, additional, ten Brinke, Anja, additional, Verstegen, Niels J M, additional, Zwinderman, Koos A H, additional, van Ham, S Marieke, additional, Rispens, Theo, additional, Kuijpers, Taco W, additional, Wolbink, Gertjan, additional, Eftimov, Filip, additional, de Jongh, Rivka, additional, Kuijper, Lisan, additional, Duurland, Mariel, additional, Hagen, Ruth, additional, van den Dijssel, Jet, additional, Kreher, Christine, additional, Bos, Amelie, additional, Palomares Cabeza, Viriginia, additional, Konijn, Veronique, additional, Elias, George, additional, Vallejo, Juan, additional, van Gils, Marrit, additional, Ashhurst, Tom, additional, Nejentsev, Sergey, additional, and Mirfazeli, Elham, additional

Published
2022
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29. Humoral responses after second and third SARS-CoV-2 vaccination in patients with immune-mediated inflammatory disorders on immunosuppressants: a cohort study

Author

Wieske, Luuk, primary, van Dam, Koos P J, additional, Steenhuis, Maurice, additional, Stalman, Eileen W, additional, Kummer, Laura Y L, additional, van Kempen, Zoé L E, additional, Killestein, Joep, additional, Volkers, Adriaan G, additional, Tas, Sander W, additional, Boekel, Laura, additional, Wolbink, Gerrit J, additional, van der Kooi, Anneke J, additional, Raaphorst, Joost, additional, Löwenberg, Mark, additional, Takkenberg, R Bart, additional, D'Haens, Geert R A M, additional, Spuls, Phyllis I, additional, Bekkenk, Marcel W, additional, Musters, Annelie H, additional, Post, Nicoline F, additional, Bosma, Angela L, additional, Hilhorst, Marc L, additional, Vegting, Yosta, additional, Bemelman, Frederike J, additional, Voskuyl, Alexandre E, additional, Broens, Bo, additional, Sanchez, Agner Parra, additional, van Els, Cécile A C M, additional, de Wit, Jelle, additional, Rutgers, Abraham, additional, de Leeuw, Karina, additional, Horváth, Barbara, additional, Verschuuren, Jan J G M, additional, Ruiter, Annabel M, additional, van Ouwerkerk, Lotte, additional, van der Woude, Diane, additional, Allaart, Renée C F, additional, Teng, Y K Onno, additional, van Paassen, Pieter, additional, Busch, Matthias H, additional, Jallah, Papay B P, additional, Brusse, Esther, additional, van Doorn, Pieter A, additional, Baars, Adája E, additional, Hijnen, Dirk Jan, additional, Schreurs, Corine R G, additional, van der Pol, W Ludo, additional, Goedee, H Stephan, additional, Keijzer, Sofie, additional, Keijser, Jim B D, additional, Boogaard, Arend, additional, Cristianawati, Olvi, additional, ten Brinke, Anja, additional, Verstegen, Niels J M, additional, Zwinderman, Koos A H, additional, van Ham, S Marieke, additional, Kuijpers, Taco W, additional, Rispens, Theo, additional, Eftimov, Filip, additional, de Jongh, R., additional, van de Sandt, C.E., additional, Kuijper, L., additional, Duurland, M., additional, Hagen, R.R., additional, van den Dijssel, J., additional, Kreher, C., additional, Bos, A., additional, Palomares Cabeza, V., additional, Konijn, V.A.L., additional, Elias, G., additional, Vallejo, J.G., additional, van Gils, M.J., additional, Ashhurst, T.M., additional, Nejentsev, S., additional, and Mirfazeli, E.S., additional

Published
2022
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30. Corrigendum: Children and Adults With Mild COVID-19: Dynamics of the Memory T Cell Response Up to 10 Months

Author

Kaaijk, Patricia, primary, Olivo Pimentel, Verónica, additional, Emmelot, Maarten E., additional, Poelen, Martien C. M., additional, Cevirgel, Alper, additional, Schepp, Rutger M., additional, den Hartog, Gerco, additional, Reukers, Daphne F. M., additional, Beckers, Lisa, additional, van Beek, Josine, additional, van Els, Cécile A. C. M., additional, Meijer, Adam, additional, Rots, Nynke Y., additional, and de Wit, Jelle, additional

Published
2022
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31. Children and Adults With Mild COVID-19: Dynamics of the Memory T Cell Response up to 10 Months

Author

Kaaijk, Patricia, primary, Olivo Pimentel, Verónica, additional, Emmelot, Maarten E., additional, Poelen, Martien C. M., additional, Cevirgel, Alper, additional, Schepp, Rutger M., additional, den Hartog, Gerco, additional, Reukers, Daphne F.M., additional, Beckers, Lisa, additional, van Beek, Josine, additional, van Els, Cécile A. C. M., additional, Meijer, Adam, additional, Rots, Nynke Y., additional, and de Wit, Jelle, additional

Published
2022
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32. SARS-CoV-2 Delta Breakthrough Infections in Vaccinated Patients with Immune-Mediated Inflammatory Diseases Using Immunosuppressants – Data from Two Controlled Prospective Cohort Studies

Author

Boekel, Laura, primary, Stalman, Eileen, additional, Wieske, Luuk, additional, Hooijberg, Femke, additional, van Dam, Koos, additional, Besten, Yaëlle, additional, Kummer, Laura, additional, Steenhuis, Maurice, additional, van Kempen, Zoé, additional, Killestein, Joep, additional, Volkers, Adriaan G., additional, Tas, Sander, additional, van der Kooi, Anneke J., additional, Raaphorst, Joost, additional, Löwenberg, Mark, additional, Takkenberg, R. Bart, additional, D&apos;Haens, Geert R.A.M., additional, Spuls, Phyllis I., additional, Bekkenk, Marcel W., additional, Musters, Annelie H., additional, Post, Nicoline F., additional, Bosma, Angela L., additional, Hilhorst, Marc L., additional, Vegting, Yosta, additional, Bemelman, Frederike J., additional, Voskuyl, Alexandre, additional, Broens, Bo, additional, Sanchez, Agner Parra, additional, van Els, Cecile, additional, de Wit, Jelle, additional, Rutgers, Abraham, additional, de Leeuw, Karina, additional, Horváth, Barbara, additional, Verschuuren, Jan J.G.M., additional, Ruiter, Annabel M., additional, van Ouwerkerk, Lotte, additional, van der Woude, Diane, additional, Allaart, Cornelia F., additional, Teng, Onno YK, additional, van Paassen, Pieter, additional, Busch, Matthias, additional, Jallah, Papay B.P., additional, Brusse, Esther, additional, van Doorn, Pieter, additional, Baars, Adája E., additional, Hijnen, Dirk Jan, additional, Schreurs, Corine R.G., additional, van der Pol, Ludo, additional, Goedee, H. Stephan, additional, Vogelzang, Erik, additional, Leeuw, Maureen, additional, Atiqi, Sadaf, additional, van Vollenhoven, Ronald, additional, Gerritsen, Martijn, additional, van der Horst-Bruinsma, Irene E., additional, Lems, Willem F., additional, Nurmohamed, Michael, additional, Boers, Maarten, additional, Keijzer, Sofie, additional, Keijser, Jim, additional, Boogaard, Arend, additional, Cristianawati, Olvi, additional, ten Brinke, Anja, additional, Verstegen, Niels, additional, Zwinderman, Aeilko H., additional, van Ham, Marieke, additional, Rispens, Theo, additional, Kuijpers, Taco, additional, Wolbink, Gertjan, additional, and Eftimov, Filip, additional

Published
2022
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34. RORγt inhibitors suppress TH17 responses in inflammatory arthritis and inflammatory bowel disease

Author

de Wit, Jelle, Al-Mossawi, Hussein M., Hühn, Michael H., Arancibia-Cárcamo, Carolina V., Doig, Karen, Kendrick, Benjamin, Gundle, Roger, Taylor, Peter, Mcclanahan, Terri, Murphy, Erin, Zhang, Hongjun, Barr, Ken, Miller, Richard J., Hu, Xiao, Aicher, Thomas D., Morgan, Rodney W., Glick, Gary D., Zaller, Dennis, Correll, Craig, Powrie, Fiona, and Bowness, Paul

Published
2016
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35. Naturally circulating pertactin-deficient strains induce distinct gene expression and inflammatory signatures in human dendritic cells

Author

Kroes, Michiel M, Miranda-Bedate, Alberto, Hovingh, Elise S, Jacobi, Ronald, Schot, Corrie, Pupo, Elder, Raeven, René H M, van der Ark, Arno A J, van Putten, Jos P M, de Wit, Jelle, Mariman, Rob, and Pinelli, Elena

Subjects
transcriptomics, Pathogen adaptation, proteomics, dendritic cell, human, RNAseq, innate immunity, pertactin
Abstract

Respiratory infections caused by Bordetella pertussis are reemerging despite high pertussis vaccination coverage. Since the introduction of the acellular pertussis vaccine in the late twentieth century, circulating B. pertussis strains increasingly lack expression of the vaccine component pertactin (Prn). In some countries, up to 90% of the circulating B. pertussis strains are deficient in Prn. To better understand the resurgence of pertussis, we investigated the response of human monocyte-derived dendritic cells (moDCs) to naturally circulating Prn-expressing (Prn-Pos) and Prn-deficient (Prn-Neg) B. pertussis strains from 2016 in the Netherlands. Transcriptome analysis of moDC showed enriched IFNα response-associated gene expression after exposure to Prn-Pos B. pertussis strains, whereas the Prn-Neg strains induced enriched expression of interleukin- and TNF-signaling genes, as well as other genes involved in immune activation. Multiplex immune assays confirmed enhanced proinflammatory cytokine secretion by Prn-Neg stimulated moDC. Comparison of the proteomes from the Prn-Pos and Prn-Neg strains revealed, next to the difference in Prn, differential expression of a number of other proteins including several proteins involved in metabolic processes. Our findings indicate that Prn-deficient B. pertussis strains induce a distinct and stronger immune activation of moDCs than the Prn-Pos strains. These findings highlight the role of pathogen adaptation in the resurgence of pertussis as well as the effects that vaccine pressure can have on a bacterial population.

Published
2021

36. Children and adults with mild COVID-19 symptoms develop memory T cell immunity to SARS-CoV-2

Author

Kaaijk, Patricia, primary, Pimentel, Verónica Olivo, additional, Emmelot, Maarten E., additional, Poelen, Martien, additional, Cevirgel, Alper, additional, Schepp, Rutger M., additional, den Hartog, Gerco, additional, Reukers, Daphne F.M., additional, Beckers, Lisa, additional, van Beek, Josine, additional, van Els, Cécile A.C.M., additional, Meijer, Adam, additional, Rots, Nynke Y., additional, and de Wit, Jelle, additional

Published
2021
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39. Latent CMV Infection Is Associated With Lower Influenza Virus-Specific Memory T-Cell Frequencies, but Not With an Impaired T-Cell Response to Acute Influenza Virus Infection

Author

van den Berg, Sara P. H., primary, Lanfermeijer, Josien, additional, Jacobi, Ronald H. J., additional, Hendriks, Marion, additional, Vos, Martijn, additional, van Schuijlenburg, Roos, additional, Nanlohy, Nening M., additional, Borghans, José A. M., additional, van Beek, Josine, additional, van Baarle, Debbie, additional, and de Wit, Jelle, additional

Published
2021
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40. Naturally circulating pertactin-deficient Bordetella pertussis strains induce distinct gene expression and inflammatory signatures in human dendritic cells

Author

Kroes, Michiel M, Miranda-Bedate, Alberto, Hovingh, Elise S, Jacobi, Ronald, Schot, Corrie, Pupo, Elder, Raeven, René H M, van der Ark, Arno A J, van Putten, Jos P M, de Wit, Jelle, Mariman, Rob, Pinelli, Elena, LS Equine Muscoskeletal Biology, Infectiebiologie, dI&I I&I-2, LS Equine Muscoskeletal Biology, Infectiebiologie, and dI&I I&I-2

Subjects
0301 basic medicine, Bordetella pertussis, dendritic cell, Whooping Cough, Epidemiology, 030106 microbiology, Immunology, Adaptation, Biological, Microbiology, pertactin, Proinflammatory cytokine, transcriptomics, 03 medical and health sciences, proteomics, Immune system, Virology, Drug Discovery, Humans, Secretion, human, Virulence Factors, Bordetella, innate immunity, Inflammation, Pertussis Vaccine, Innate immune system, biology, Gene Expression Profiling, Interleukin, Dendritic Cells, General Medicine, Dendritic cell, RNAseq, biology.organism_classification, Toll-Like Receptor 2, Toll-Like Receptor 4, Pathogen adaptation, 030104 developmental biology, Infectious Diseases, Host-Pathogen Interactions, Cytokines, Parasitology, Pertactin, Transcriptome, Research Article, Bacterial Outer Membrane Proteins
Abstract

Respiratory infections caused by Bordetella pertussis are reemerging despite high pertussis vaccination coverage. Since the introduction of the acellular pertussis vaccine in the late twentieth century, circulating B. pertussis strains increasingly lack expression of the vaccine component pertactin (Prn). In some countries, up to 90% of the circulating B. pertussis strains are deficient in Prn. To better understand the resurgence of pertussis, we investigated the response of human monocyte-derived dendritic cells (moDCs) to naturally circulating Prn-expressing (Prn-Pos) and Prn-deficient (Prn-Neg) B. pertussis strains from 2016 in the Netherlands. Transcriptome analysis of moDC showed enriched IFNα response-associated gene expression after exposure to Prn-Pos B. pertussis strains, whereas the Prn-Neg strains induced enriched expression of interleukin- and TNF-signaling genes, as well as other genes involved in immune activation. Multiplex immune assays confirmed enhanced proinflammatory cytokine secretion by Prn-Neg stimulated moDC. Comparison of the proteomes from the Prn-Pos and Prn-Neg strains revealed, next to the difference in Prn, differential expression of a number of other proteins including several proteins involved in metabolic processes. Our findings indicate that Prn-deficient B. pertussis strains induce a distinct and stronger immune activation of moDCs than the Prn-Pos strains. These findings highlight the role of pathogen adaptation in the resurgence of pertussis as well as the effects that vaccine pressure can have on a bacterial population.

Published
2021
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41. Naturally circulating pertactin-deficient Bordetella pertussis strains induce distinct gene expression and inflammatory signatures in human dendritic cells

Author

LS Equine Muscoskeletal Biology, Infectiebiologie, dI&I I&I-2, Kroes, Michiel M, Miranda-Bedate, Alberto, Hovingh, Elise S, Jacobi, Ronald, Schot, Corrie, Pupo, Elder, Raeven, René H M, van der Ark, Arno A J, van Putten, Jos P M, de Wit, Jelle, Mariman, Rob, Pinelli, Elena, LS Equine Muscoskeletal Biology, Infectiebiologie, dI&I I&I-2, Kroes, Michiel M, Miranda-Bedate, Alberto, Hovingh, Elise S, Jacobi, Ronald, Schot, Corrie, Pupo, Elder, Raeven, René H M, van der Ark, Arno A J, van Putten, Jos P M, de Wit, Jelle, Mariman, Rob, and Pinelli, Elena

Published
2021

42. Longitudinal characterization of the mumps-specific HLA-A2 restricted T-cell response after mumps virus infection

Author

CTI Luminex, CTI Borghans, Infection & Immunity, CTI Research, Lanfermeijer, Josien, Nühn, Marieke M., Emmelot, Maarten E., Poelen, Martien C.M., van Els, Cécile A.C.M., Borghans, José A.M., van Baarle, Debbie, Kaaijk, Patricia, de Wit, Jelle, CTI Luminex, CTI Borghans, Infection & Immunity, CTI Research, Lanfermeijer, Josien, Nühn, Marieke M., Emmelot, Maarten E., Poelen, Martien C.M., van Els, Cécile A.C.M., Borghans, José A.M., van Baarle, Debbie, Kaaijk, Patricia, and de Wit, Jelle

Published
2021

43. Latent CMV Infection Is Associated With Lower Influenza Virus-Specific Memory T-Cell Frequencies, but Not With an Impaired T-Cell Response to Acute Influenza Virus Infection

Author

CTI Luminex, CTI Borghans, Infection & Immunity, CTI Research, van den Berg, Sara P.H., Lanfermeijer, Josien, Jacobi, Ronald H.J., Hendriks, Marion, Vos, Martijn, van Schuijlenburg, Roos, Nanlohy, Nening M., Borghans, José A.M., van Beek, Josine, van Baarle, Debbie, de Wit, Jelle, CTI Luminex, CTI Borghans, Infection & Immunity, CTI Research, van den Berg, Sara P.H., Lanfermeijer, Josien, Jacobi, Ronald H.J., Hendriks, Marion, Vos, Martijn, van Schuijlenburg, Roos, Nanlohy, Nening M., Borghans, José A.M., van Beek, Josine, van Baarle, Debbie, and de Wit, Jelle

Published
2021

44. Co-Expression of TIGIT and Helios Marks Immunosenescent CD8+ T Cells During Aging.

Author

Pieren, Daan K. J., Smits, Noortje A. M., Postel, Rimke J., Kandiah, Vinitha, de Wit, Jelle, van Beek, Josine, van Baarle, Debbie, and Guichelaar, Teun

Subjects
T cells, CELLULAR aging, OLDER people, CELL differentiation, IMMUNOSENESCENCE
Abstract

Aging leads to alterations in the immune system that result in ineffective responsiveness against pathogens. Features of this process, collectively known as immunosenescence, accumulate in CD8+ T cells with age and have been ascribed to differentiation of these cells during the course of life. Here we aimed to identify novel markers in CD8+ T cells associated with immunosenescence. Furthermore, we assessed how these markers relate to the aging-related accumulation of highly differentiated CD27-CD28- cells. We found that co-expression of the transcription factor Helios and the aging-related marker TIGIT identifies CD8+ T cells that fail to proliferate and show impaired induction of activation markers CD69 and CD25 in response to stimulation in vitro. Despite this, in blood of older adults we found TIGIT+Helios+ T cells to become highly activated during an influenza-A virus infection, but these higher frequencies of activated TIGIT+Helios+ T cells associate with longer duration of coughing. Moreover, in healthy individuals, we found that TIGIT+Helios+ CD8+ T cells accumulate with age in the highly differentiated CD27-CD28- population. Interestingly, TIGIT+Helios+ CD8+ T cells also accumulate with age among the less differentiated CD27+CD28- T cells before their transit into the highly differentiated CD27-CD28- stage. This finding suggests that T cells with immunosenescent features become prominent at old age also within the earlier differentiation states of these cells. Our findings show that co-expression of TIGIT and Helios refines the definition of immunosenescent CD8+ T cells and challenge the current dogma of late differentiation stage as proxy for T-cell immunosenescence. [ABSTRACT FROM AUTHOR]

Published
2022
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45. Measles Vaccination Elicits a Polyfunctional Antibody Response, Which Decays More Rapidly in Early Vaccinated Children.

Author

Brinkman, Iris D, Butler, Audrey L, Wit, Jelle de, Binnendijk, Rob S van, Alter, Galit, Baarle, Debbie van, de Wit, Jelle, van Binnendijk, Rob S, and van Baarle, Debbie

Subjects
MEASLES, IMMUNIZATION, ANTIBODY formation, RESEARCH funding, PARAMYXOVIRUSES, VIRAL antibodies, MEASLES vaccines
Abstract

Background: Measles outbreaks are reported worldwide and pose a serious threat, especially to young unvaccinated infants. Early measles vaccination given to infants under 12 months of age can induce protective antibody levels, but the long-term antibody functionalities are unknown.Methods: Measles-specific antibody functionality was tested using a systems serology approach for children who received an early measles vaccination at 6-8 or 9-12 months, followed by a regular dose at 14 months of age, and children who only received the vaccination at 14 months. Antibody functionalities comprised complement deposition, cellular cytotoxicity, and neutrophil and cellular phagocytosis. We used Pearson's r correlations between all effector functions to investigate the coordination of the response.Results: Children receiving early measles vaccination at 6-8 or 9-12 months of age show polyfunctional antibody responses. Despite significant lower levels of antibodies in these early-vaccinated children, Fc effector functions were comparable with regular-timed vaccinees at 14 months. However, 3-year follow-up revealed significant decreased polyfunctionality in children who received a first vaccination at 6-8 months of age, but not in children who received the early vaccination at 9-12 months.Conclusions: Antibodies elicited in early-vaccinated children are equally polyfunctional to those elicited from children who received vaccination at 14 months. However, these antibody functionalities decay more rapidly than those induced later in life, which may lead to suboptimal, long-term protection. [ABSTRACT FROM AUTHOR]

Published
2022
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47. Associations of faecal microbiota with influenza-like illness in participants aged 60 years or older: an observational study

Author

Fuentes, Susana, primary, den Hartog, Gerco, additional, Nanlohy, Nening M, additional, Wijnands, Lucas, additional, Ferreira, José A, additional, Nicolaie, Mioara A, additional, Pennings, Jeroen L A, additional, Jacobi, Ronald, additional, de Wit, Jelle, additional, van Beek, Josine, additional, and van Baarle, Debbie, additional

Published
2021
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49. Activation of Human NK Cells by Requires Inflammasome Activation in Macrophages

Author

Kroes, Michiel M, Mariman, Rob, Hijdra, Daniëlle, Hamstra, Hendrik-Jan, van Boxtel, Karlijn J W M, van Putten, Jos P M, de Wit, Jelle, and Pinelli, Elena

Subjects
NLRP3, inflammasome, human, interferon-gamma, innate immunity, Bordetella pertussis, interleukin-18, crosstalk
Abstract

Pertussis is a highly contagious respiratory infection caused by the bacterium Bordetella pertussis. Humans are the only known natural reservoir of B. pertussis. In mice, macrophages and NK cells have a key role in confining B. pertussis to the respiratory tract. However, the mechanisms underlying this process, particularly during human infections, remain unclear. Here we characterized the activation of human macrophages and NK cells in response to B. pertussis and unraveled the role of inflammasomes in this process. NLRP3 inflammasome activation by B. pertussis in human macrophage-like THP-1 cells and primary monocyte-derived macrophages (mo-MΦ) was shown by the visualization of ASC-speck formation, pyroptosis, and the secretion of caspase-mediated IL-1β and IL-18. In contrast to macrophages, stimulation of human CD56+CD3- NK cells by B. pertussis alone did not result in activation of these cells. However, co-culture of B. pertussis-stimulated mo-MΦ and autologous NK cells resulted in high amounts of IFNγ secretion and an increased frequency of IL-2Rα+ and HLA-DR+ NK cells, indicating NK cell activation. This activation was significantly reduced upon inhibition of inflammasome activity or blocking of IL-18 in the mo-MΦ/NK cell co-culture. Furthermore, we observed increased secretion of proinflammatory cytokines in the B. pertussis-stimulated mo-MΦ/NK co-culture compared to the mo-MΦ single culture. Our results demonstrate that B. pertussis induces inflammasome activation in human macrophages and that the IL-18 produced by these cells is required for the activation of human NK cells, which in turn enhances the pro-inflammatory response to this pathogen. Our data provides a better understanding of the underlying mechanisms involved in the induction of innate immune responses against B. pertussis. These findings contribute to the knowledge required for the development of improved intervention strategies to control this highly contagious disease.

Published
2019

50. Activation of Human NK Cells by Bordetella pertussis Requires Inflammasome Activation in Macrophages

Author

Kroes, Michiel M, Mariman, Rob, Hijdra, Daniëlle, Hamstra, Hendrik-Jan, van Boxtel, Karlijn J W M, van Putten, Jos P M, de Wit, Jelle, Pinelli, Elena, Faculteit Diergeneeskunde, LS Infectiebiologie (Bacteriologie), dI&I I&I-2, Faculteit Diergeneeskunde, LS Infectiebiologie (Bacteriologie), and dI&I I&I-2

Subjects
lcsh:Immunologic diseases. Allergy, 0301 basic medicine, Bordetella pertussis, Bordetellapertussis, Immunology, interleukin-18, crosstalk, Proinflammatory cytokine, 03 medical and health sciences, 0302 clinical medicine, NLRP3, inflammasome, medicine, Immunology and Allergy, Interferon gamma, Secretion, human, innate immunity, Innate immune system, biology, Chemistry, Pyroptosis, Respiratory infection, Inflammasome, biology.organism_classification, 030104 developmental biology, interferon-gamma, lcsh:RC581-607, 030215 immunology, medicine.drug
Abstract

Pertussis is a highly contagious respiratory infection caused by the bacterium Bordetella pertussis. Humans are the only known natural reservoir of B. pertussis. In mice, macrophages and NK cells have a key role in confining B. pertussis to the respiratory tract. However, the mechanisms underlying this process, particularly during human infections, remain unclear. Here we characterized the activation of human macrophages and NK cells in response to B. pertussis and unraveled the role of inflammasomes in this process. NLRP3 inflammasome activation by B. pertussis in human macrophage-like THP-1 cells and primary monocyte-derived macrophages (mo-MΦ) was shown by the visualization of ASC-speck formation, pyroptosis, and the secretion of caspase-mediated IL-1β and IL-18. In contrast to macrophages, stimulation of human CD56+CD3- NK cells by B. pertussis alone did not result in activation of these cells. However, co-culture of B. pertussis-stimulated mo-MΦ and autologous NK cells resulted in high amounts of IFNγ secretion and an increased frequency of IL-2Rα+ and HLA-DR+ NK cells, indicating NK cell activation. This activation was significantly reduced upon inhibition of inflammasome activity or blocking of IL-18 in the mo-MΦ/NK cell co-culture. Furthermore, we observed increased secretion of proinflammatory cytokines in the B. pertussis-stimulated mo-MΦ/NK co-culture compared to the mo-MΦ single culture. Our results demonstrate that B. pertussis induces inflammasome activation in human macrophages and that the IL-18 produced by these cells is required for the activation of human NK cells, which in turn enhances the pro-inflammatory response to this pathogen. Our data provides a better understanding of the underlying mechanisms involved in the induction of innate immune responses against B. pertussis. These findings contribute to the knowledge required for the development of improved intervention strategies to control this highly contagious disease.

Published
2019

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