30 results on '"de Vogel-van den Bosch J"'
Search Results
2. Variability of Food Choice Motives: Two Dutch studies showing variation across meal moment, location and social context
- Author
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Verain, M.C.D., primary, van den Puttelaar, J., additional, Zandstra, E.H., additional, Lion, R., additional, de Vogel-van den Bosch, J., additional, Hoonhout, H.C.M., additional, and Onwezen, M.C., additional
- Published
- 2022
- Full Text
- View/download PDF
3. Healthy aging and muscle function are positively associated with NAD(+) abundance in humans
- Author
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Janssens, G.E., Grevendonk, L., Perez, R.Z., Schomakers, B.V., de Vogel-van den Bosch, J., Geurts, J.M.W., van Weeghel, M., Schrauwen, P., Houtkooper, R.H., Hoeks, J., Janssens, G.E., Grevendonk, L., Perez, R.Z., Schomakers, B.V., de Vogel-van den Bosch, J., Geurts, J.M.W., van Weeghel, M., Schrauwen, P., Houtkooper, R.H., and Hoeks, J.
- Abstract
Skeletal muscle is greatly affected by aging, resulting in a loss of metabolic and physical function. However, the underlying molecular processes and how (lack of) physical activity is involved in age-related metabolic decline in muscle function in humans is largely unknown. Here, we compared, in a cross-sectional study, the muscle metabolome from young to older adults, whereby the older adults were exercise trained, had normal physical activity levels or were physically impaired. Nicotinamide adenine dinucleotide (NAD(+)) was one of the most prominent metabolites that was lower in older adults, in line with preclinical models. This lower level was even more pronounced in impaired older individuals, and conversely, exercise-trained older individuals had NAD(+) levels that were more similar to those found in younger individuals. NAD(+) abundance positively correlated with average number of steps per day and mitochondrial and muscle functioning. Our work suggests that a clear association exists between NAD(+) and health status in human aging.
- Published
- 2022
4. Variability of Food Choice Motives: Two Dutch studies showing variation across meal moment, location and social context
- Author
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Verain, M.C.D., Van Den Puttelaar, J., Zandstra, E.H., Lion, R., De Vogel-van Den Bosch, J., Hoonhout, H.C.M., Onwezen, M.C., Verain, M.C.D., Van Den Puttelaar, J., Zandstra, E.H., Lion, R., De Vogel-van Den Bosch, J., Hoonhout, H.C.M., and Onwezen, M.C.
- Abstract
Food Choice Motives (FCMs) such as price, sensory appeal and health are important in understanding food consumption. FCMs are traditionally investigated at a general level, for food choices on ‘a typical day’. However, food choices have been shown to differ across temporal, situational and social contexts. This suggests that measuring FCMs at a context-specific level could increase our understanding of food consumption in different contexts. Therefore, the current paper aims to explore whether FCMs are indeed context-specific for different meal moments, locations and social contexts. Two studies were conducted among Dutch adults (Study 1: N = 935; Study 2: N = 642). Both studies measured FCMs in context, either by using 2-hour recalls (Study 1) or recalls of the last consumption moment (Study 2). Result showed that participants rated and ranked FCMs significantly different across most contexts showing the relevance of considering the context when studying FCMs. Egocentric motives of taste, affordability, and convenience were the most important motives across all contexts, as was health. In contrast, sustainability-related motives were consistently rated as least important. Most variability occurred in the middle part of the rankings and mainly in health-related motives such as weight control and safety. This shows the added value of measuring FCMs in different contexts, particularly for health-related motives. The contexts snacking versus main meals, eating out of home versus at home and eating alone versus with others showed the most pronounced contrasts in ranking of FCMs. The current study is the first to quantitatively explore the variability of FCMs across eating contexts, both in rating and ranking of FCMs. The chosen research method resulted in a representative, though unbalanced sample of consumption contexts in the Netherlands, which limits the generalizability of the results to an international context and restricts the insights in out-of-home contexts as
- Published
- 2022
5. Increased species richness in the oral microbiome by a 13-week protein and combined lifestyle intervention in older adults with obesity and type 2 diabetes
- Author
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Haji, M., primary, Memelink, R.G., additional, Brandt, B.W., additional, Buijs, M.J., additional, Pasman, W.J., additional, De Vogel-Van den Bosch, J., additional, Wopereis, S., additional, Zaura, E., additional, and Weijs, P.J., additional
- Published
- 2021
- Full Text
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6. Impact of aging and exercise on skeletal muscle mitochondrial capacity, energy metabolism, and physical function
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Grevendonk, L, Grevendonk, L, Connell, N J, McCrum, C, Fealy, C E, Bilet, L, Bruls, Y M H, Mevenkamp, J, Schrauwen-Hinderling, V B, Jörgensen, J A, Moonen-Kornips, E, Schaart, G, Havekes, B, de Vogel-van den Bosch, J, Bragt, M C E, Meijer, K, Schrauwen, P, Hoeks, J, Grevendonk, L, Grevendonk, L, Connell, N J, McCrum, C, Fealy, C E, Bilet, L, Bruls, Y M H, Mevenkamp, J, Schrauwen-Hinderling, V B, Jörgensen, J A, Moonen-Kornips, E, Schaart, G, Havekes, B, de Vogel-van den Bosch, J, Bragt, M C E, Meijer, K, Schrauwen, P, and Hoeks, J
- Abstract
The relationship between the age-associated decline in mitochondrial function and its effect on skeletal muscle physiology and function remain unclear. In the current study, we examined to what extent physical activity contributes to the decline in mitochondrial function and muscle health during aging and compared mitochondrial function in young and older adults, with similar habitual physical activity levels. We also studied exercise-trained older adults and physically impaired older adults. Aging was associated with a decline in mitochondrial capacity, exercise capacity and efficiency, gait stability, muscle function, and insulin sensitivity, even when maintaining an adequate daily physical activity level. Our data also suggest that a further increase in physical activity level, achieved through regular exercise training, can largely negate the effects of aging. Finally, mitochondrial capacity correlated with exercise efficiency and insulin sensitivity. Together, our data support a link between mitochondrial function and age-associated deterioration of skeletal muscle. Aging is associated with a progressive loss of muscle function. Here the authors characterize mitochondrial capacity and muscle function in young and older adults with similar habitual physical activity and also compared to older adults with exercise training or with physical impairment.
- Published
- 2021
7. Sustained changes in body composition during 6 months follow-up after combined lifestyle intervention in older adults with obesity and type 2 diabetes
- Author
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Memelink, RG, Pasman, WJ, Valentin, B, de Vogel - van den Bosch, J, Weijs, PJM, Lectoraat Voeding en Beweging, and Kenniscentrum Bewegen, Sport en Voeding
- Published
- 2019
8. OR33: Preservation of Lean Mass Upon Combined Lifestyle Intervention in Older Adults with Obesity and Type 2 Diabetes During 6-Months Follow-Up After RCT (Probe Study)
- Author
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Memelink, R.G., primary, Bongers, A., additional, Pasman, W.J., additional, van Rijmenam, M.J., additional, Wopereis, S., additional, Verlaan, G., additional, De Vogel-Van Den Bosch, J., additional, and Weijs, P.J.M., additional
- Published
- 2019
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9. Energy intake and expenditure in obese older adults with and without type 2 diabetes
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Memelink, R.G., Verreijen, A.M., de Vogel-van den Bosch, J., Weijs, P.J., Deutz, N.E.P., Mays, J.A., Roeske, S.C., and Lectoraat Voeding en Beweging
- Subjects
musculoskeletal diseases - Abstract
Rationale: Obesity is a risk factor for type 2 diabetes (DM2), however not all obese people develop DM2. We explored differences in energy intake and expenditure between obese older adults with and without DM2. Methods: Baseline data from 2 lifestyle interventions with a total of 202 obese older adults were included in the analyses. Obesity was defined as BMI > 30.0, or >27.0 with waist circumference >88 (women) or >102 cm (men). DM2 was confirmed by use of diabetes medication. Subjects were between 55 and 85 years old and 45% was female. Energy intake (EI) was measured by 3-day food diary and physical activity level (PAL) by 3-day movement diary. Resting energy expenditure (REE) was measured using indirect calorimetry and total energy expenditure (TEE) was calculated as REE x PAL. Between group differences were analysed with independent samples T-tests. Results: The obese group with DM2 (n = 117) had more males (67.5% vs 37.6% p < 0.001) and similar BMI (33.3 vs 33.0 kg/m2) compared to the group without DM2 (n = 85). Analyses of males and females separately showed lower PAL in males with DM2 (vs without DM2; 1.37 vs 1.45, p = 0.015), without differences in EI (2055 vs 1953 kcal/d), REE (1970 vs 1929 kcal/d), and TEE (2699 vs 2830 kcal/d). In females with DM2, both PAL (1.38 vs 1.47, p = 0.014) and EI (1543 vs 1839 kcal/d, p = 0.008) were significantly lower, whereas REE (1592 vs 1598 kcal/d) and TEE (2220 vs 2318 kcal/d) did not differ significantly from obese females without DM2. Conclusion: In both males and females, obese older adults with type 2 diabetes showed similar resting and total energy expenditure but lower physical activity level compared to those without DM2. Females with DM2 showed lower energy intake. On average, subjects seem to have a negative energy balance, which is probably due to a combination of underreporting of intake and over-reporting of activity.
- Published
- 2017
10. Effect of a whey protein drink enriched with leucine and vitamin D on lean mass and glycemic control during a lifestyle intervention in obese older adults with (pre-)diabetes type 2: A double-blind RCT
- Author
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Memelink, R., primary, Pasman, W., additional, Bongers, A., additional, Tump, A., additional, van Ginkel, A., additional, Tromp, W., additional, Wopereis, S., additional, Verlaan, S., additional, de Vogel-van den Bosch, J., additional, and Weijs, P., additional
- Published
- 2018
- Full Text
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11. MON-P144: Energy Intake and Expenditure in Obese Older Adults with and Without Type 2 Diabetes
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Memelink, R.G., primary, Verreijen, A.M., additional, De Vogel-Van den Bosch, J., additional, and Weijs, P.J., additional
- Published
- 2017
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12. The effects of long- or medium-chain fat diets on glucose tolerance and myocellular content of lipid intermediates in rats.
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de Vogel-van den Bosch, J., de Vogel-van den Bosch, J., Hoeks, J., Timmers, S., Houten, S.M., van Dijk, P.J., Boon, W., Van Beurden, D., Schaart, G., Kersten, S., Voshol, P.J., Wanders, R.J., Hesselink, M.K.C., Schrauwen, P., de Vogel-van den Bosch, J., de Vogel-van den Bosch, J., Hoeks, J., Timmers, S., Houten, S.M., van Dijk, P.J., Boon, W., Van Beurden, D., Schaart, G., Kersten, S., Voshol, P.J., Wanders, R.J., Hesselink, M.K.C., and Schrauwen, P.
- Abstract
Accumulation of triacylglycerols (TAGs) and acylcarnitines in skeletal muscle upon high-fat (HF) feeding is the resultant of fatty acid uptake and oxidation and is associated with insulin resistance. As medium-chain fatty acids (MCFAs) are preferentially beta-oxidized over long-chain fatty acids, we examined the effects of medium-chain TAGs (MCTs) and long-chain TAGs (LCTs) on muscle lipid storage and whole-body glucose tolerance. Rats fed a low-fat (LF), HFLCT, or an isocaloric HFMCT diet displayed a similar body weight gain over 8 weeks of treatment. Only HFLCT increased myocellular TAG (42.3 +/- 4.9, 71.9 +/- 6.7, and 48.5 +/- 6.5 micromol/g for LF, HFLCT, and HFMCT, respectively, P < 0.05) and long-chain acylcarnitine content (P < 0.05). Neither HF diet increased myocellular diacylglycerol (DAG) content. Intraperitoneal (IP) glucose tolerance tests (1.5 g/kg) revealed a significantly decreased glucose tolerance in the HFMCT compared to the HFLCT-fed rats (802 +/- 40, 772 +/- 18, and 886 +/- 18 area under the curve for LF, HFLCT, and HFMCT, respectively, P < 0.05). Finally, no differences in myocellular insulin signaling after bolus insulin injection (10 U/kg) were observed between LF, HFLCT, or HFMCT-fed rats. These results show that accumulation of TAGs and acylcarnitines in skeletal muscle in the absence of body weight gain do not impede myocellular insulin signaling or whole-body glucose intolerance.
- Published
- 2011
13. High-fat diets rich in medium- versus long-chain fatty acids induce distinct patterns of tissue specific insulin resistance.
- Author
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de Vogel-van den Bosch, J., de Vogel-van den Bosch, J., van den Berg, S.A., Bijland, S., Voshol, P.J., Havekes, L.M., Romijn, H.A., Hoeks, J., Van Beurden, D., Hesselink, M.K.C., Schrauwen, P., van Dijk, K.W., de Vogel-van den Bosch, J., de Vogel-van den Bosch, J., van den Berg, S.A., Bijland, S., Voshol, P.J., Havekes, L.M., Romijn, H.A., Hoeks, J., Van Beurden, D., Hesselink, M.K.C., Schrauwen, P., and van Dijk, K.W.
- Abstract
Excess dietary long-chain fatty acid (LCFA) intake results in ectopic lipid accumulation and insulin resistance. Since medium-chain fatty acids (MCFA) are preferentially oxidized over LCFA, we hypothesized that diets rich in MCFA result in a lower ectopic lipid accumulation and insulin resistance compared to diets rich in LCFA. Feeding mice high-fat (HF) (45% kcal fat) diets for 8 weeks rich in triacylglycerols composed of MCFA (HFMCT) or LCFA (HFLCT) revealed a lower body weight gain in the HFMCT-fed mice. Indirect calorimetry revealed higher fat oxidation on HFMCT compared to HFLCT (0.011.0+/-0.0007 vs. 0.0096+/-0.0015 kcal/g body weight per hour, P<.05). In line with this, neutral lipid immunohistochemistry revealed significantly lower lipid storage in skeletal muscle (0.05+/-0.08 vs. 0.30+/-0.23 area%, P <.05) and in liver (0.9+/-0.4 vs. 6.4+/-0.8 area%, P<.05) after HFMCT vs. HFLCT, while ectopic fat storage in low fat (LF) was very low. Hyperinsulinemic euglycemic clamps revealed that the HFMCT and HFLCT resulted in severe whole body insulin resistance (glucose infusion rate: 53.1+/-6.8, 50.8+/-15.3 vs. 124.6+/-25.4 mumol min(-1) kg(-1), P<.001 in HFMCT, HFLCT and LF-fed mice, respectively). However, under hyperinsulinemic conditions, HFMCT revealed a lower endogenous glucose output (22.6+/-8.0 vs. 34.7+/-8.5 mumol min(-1) kg(-1), P<.05) and a lower peripheral glucose disappearance (75.7+/-7.8 vs. 93.4+/-12.4 mumol min(-1) kg(-1), P<.03) compared to HFLCT-fed mice. In conclusion, both HF diets induced whole body insulin resistance compared to LF. However, the HFMCT gained less weight, had less ectopic lipid accumulation, while peripheral insulin resistance was more pronounced compared to HFLCT. This suggests that HF-diets rich in medium- versus long-chain triacylglycerols induce insulin resistance via distinct mechanisms.
- Published
- 2011
14. Paradoxical Increase in TAG and DAG Content Parallel the Insulin Sensitizing Effect of Unilateral DGAT1 Overexpression in Rat Skeletal Muscle
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Timmers, S., de Vogel-van den Bosch, J., Hesselink, M.K.C., Van Beurden, D., Schaart, G., Ferraz, M.J., Losen, M.R., Perez - Martinez, P., de Baets, M.H.V., Aerts, J.M., Schrauwen, P., Timmers, S., de Vogel-van den Bosch, J., Hesselink, M.K.C., Van Beurden, D., Schaart, G., Ferraz, M.J., Losen, M.R., Perez - Martinez, P., de Baets, M.H.V., Aerts, J.M., and Schrauwen, P.
- Abstract
BACKGROUND: The involvement of muscle triacylglycerol (TAG) storage in the onset of insulin resistance is questioned and the attention has shifted towards inhibition of insulin signalling by the lipid intermediate diacylglycerol (DAG). The enzyme 1,2-acylCoA:diacylglyceroltransferase-1 (DGAT1) esterifies a fatty acyl-CoA on DAG to form TAG. Therefore, the aim of the present study was to investigate if unilateral overexpression of DGAT1 in adult rat Tibialis anterior (TA) muscle will increase conversion of the lipid intermediate DAG into TAG, thereby improving muscle insulin sensitivity. METHODOLOGY/PRINCIPAL FINDINGS: The DGAT1 gene construct was injected in the left TA muscle of male rats on chow or high-fat (45% kcal) diet for three weeks, followed by application of one 800 V/cm and four 80 V/cm pulses, using the contralateral leg as sham-electroporated control. Seven days after electroporation, muscle specific insulin sensitivity was assessed with a hyperinsulinemic euglycemic clamp using 2-deoxy-[3H]glucose. Here, we provide evidence that unilateral overexpression of DGAT1 in TA muscle of male rats is associated with an increased rather than decreased DAG content. Strikingly, this increase in DAG content was accompanied by improved muscle insulin sensitivity. Interestingly, markers of muscle lipolysis and mitochondrial function were also increased in DGAT1 overexpressing muscle. CONCLUSIONS/SIGNIFICANCE: We conclude that unilateral DGAT1 overexpression can rescue insulin sensitivity, possibly by increasing DAG and TAG turnover in skeletal muscle. In case of a proper balance between the supply and oxidation of fatty acids in skeletal muscle, the lipid intermediate DAG may not exert harmful effects on insulin signalling.
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- 2011
15. Differential effects of saturated versus unsaturated dietary fatty acids on weight gain and myocellular lipid profiles in mice
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Timmers, S, primary, de Vogel-van den Bosch, J, additional, de Wit, N, additional, Schaart, G, additional, van Beurden, D, additional, Hesselink, M, additional, van der Meer, R, additional, and Schrauwen, P, additional
- Published
- 2011
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16. The effect of a combined lifestyle intervention with and without protein drink on inflammation in older adults with obesity and type 2 diabetes.
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Memelink RG, Njemini R, de Bos Kuil MJJ, Wopereis S, de Vogel-van den Bosch J, Schoufour JD, Tieland M, Weijs PJM, and Bautmans I
- Subjects
- Humans, Male, Female, Aged, Middle Aged, Resistance Training methods, Diet, Reducing methods, Cytokines blood, Life Style, Diabetes Mellitus, Type 2 therapy, Diabetes Mellitus, Type 2 blood, Inflammation blood, Obesity therapy, Obesity blood, Biomarkers blood
- Abstract
Background: Chronic low-grade inflammatory profile (CLIP) is one of the pathways involved in type 2 diabetes (T2D). Currently, there is limited evidence for ameliorating effects of combined lifestyle interventions on CLIP in type 2 diabetes. We investigated whether a 13-week combined lifestyle intervention, using hypocaloric diet and resistance exercise plus high-intensity interval training with or without consumption of a protein drink, affected CLIP in older adults with T2D., Methods: In this post-hoc analysis of the PROBE study 114 adults (≥55 years) with obesity and type 2 (pre-)diabetes had measurements of C-reactive protein (CRP), pro-inflammatory cytokines interleukin (IL)-6, tumor-necrosis-factor (TNF)-α, and monocyte chemoattractant protein (MCP)-1, anti-inflammatory cytokines IL-10, IL-1 receptor antagonist (RA), and soluble tumor-necrosis-factor receptor (sTNFR)1, adipokines leptin and adiponectin, and glycation biomarkers carboxymethyl-lysine (CML) and soluble receptor for advanced glycation end products (sRAGE) from fasting blood samples. A linear mixed model was used to evaluate change in inflammatory biomarkers after lifestyle intervention and effect of the protein drink. Linear regression analysis was performed with parameters of body composition (by dual-energy X-ray absorptiometry) and parameters of insulin resistance (by oral glucose tolerance test)., Results: There were no significant differences in CLIP responses between the protein and the control groups. For all participants combined, IL-1RA, leptin and adiponectin decreased after 13 weeks (p = 0.002, p < 0.001 and p < 0.001), while ratios TNF-α/IL-10 and TNF-α/IL-1RA increased (p = 0.003 and p = 0.035). CRP increased by 12 % in participants with low to average CLIP (pre 1.91 ± 0.39 mg/L, post 2.13 ± 1.16 mg/L, p = 0.006) and decreased by 36 % in those with high CLIP (pre 5.14 mg/L ± 1.20, post 3.30 ± 2.29 mg/L, p < 0.001). Change in leptin and IL-1RA was positively associated with change in fat mass (β = 0.133, p < 0.001; β = 0.017, p < 0.001) and insulin resistance (β = 0.095, p = 0.024; β = 0.020, p = 0.001). Change in lean mass was not associated with any of the biomarkers., Conclusion: 13 weeks of combined lifestyle intervention, either with or without protein drink, reduced circulating adipokines and anti-inflammatory cytokine IL-1RA, and increased inflammatory ratios TNF-α/IL-10 and TNF-α/IL-1RA in older adults with obesity and T2D. Effect on CLIP was inversely related to baseline inflammatory status., Competing Interests: Declaration of competing interest JdV-vdB is an employee of Danone Nutricia Research. SW is an employee of the Netherlands Organisation for Applied Scientific Research (TNO), which is a not-for-profit research organisation collaborating in several public-private partnerships or business-to-business research projects that receive funding from companies. All other authors declare no conflicts of interest related to this research., (Copyright © 2024 The Authors. Published by Elsevier Inc. All rights reserved.)
- Published
- 2024
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17. Validation of the smartphone-based dietary assessment tool "Traqq" for assessing actual dietary intake by repeated 2-h recalls in adults: comparison with 24-h recalls and urinary biomarkers.
- Author
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Lucassen DA, Brouwer-Brolsma EM, Boshuizen HC, Mars M, de Vogel-Van den Bosch J, and Feskens EJ
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- Humans, Adult, Reproducibility of Results, Surveys and Questionnaires, Diet methods, Eating, Biomarkers urine, Mental Recall, Nitrogen, Energy Intake, Nutrition Assessment, Smartphone
- Abstract
Background: Conventional dietary assessment methods are affected by measurement errors. We developed a smartphone-based 2-h recall (2hR) methodology to reduce participant burden and memory-related bias., Objective: Assessing the validity of the 2hR method against traditional 24-h recalls (24hRs) and objective biomarkers., Methods: Dietary intake was assessed in 215 Dutch adults on 6 randomly selected nonconsecutive days (i.e., 3 2hR-days and 3 24hRs) during a 4-wk period. Sixty-three participants provided 4 24-h urine samples, to assess urinary nitrogen and potassium concentrations., Results: Intake estimates of energy (2052±503 kcal vs. 1976±483 kcal) and nutrients (e.g., protein: 78±23 g vs. 71±19 g; fat: 84±30 g vs. 79±26 g; carbohydrates: 220±60 g vs. 216±60 g) were slightly higher with 2hR-days than with 24hRs. Comparing self-reported protein and potassium intake to urinary nitrogen and potassium concentrations indicated a slightly higher accuracy of 2hR-days than 24hRs (protein: -14% vs. -18%; potassium: -11% vs. -16%). Correlation coefficients between methods ranged from 0.41 to 0.75 for energy and macronutrients and from 0.41 to 0.62 for micronutrients. Generally, regularly consumed food groups showed small differences in intake (<10%) and good correlations (>0.60). Intake of energy, nutrients, and food groups showed similar reproducibility (intraclass correlation coefficient) for 2hR-days and 24hRs., Conclusions: Comparing 2hR-days with 24hRs showed a relatively similar group-level bias for energy, most nutrients, and food groups. Differences were mostly due to higher intake estimates by 2hR-days. Biomarker comparisons showed less underestimation by 2hR-days as compared with 24hRs, suggesting that 2hR-days are a valid approach to assess the intake of energy, nutrients, and food groups. This trial was registered at the Dutch Central Committee on Research Involving Human Subjects (CCMO) registry as ABR. No. NL69065.081.19., (Copyright © 2023 The Authors. Published by Elsevier Inc. All rights reserved.)
- Published
- 2023
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18. Cardiometabolic health improvements upon dietary intervention are driven by tissue-specific insulin resistance phenotype: A precision nutrition trial.
- Author
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Trouwborst I, Gijbels A, Jardon KM, Siebelink E, Hul GB, Wanders L, Erdos B, Péter S, Singh-Povel CM, de Vogel-van den Bosch J, Adriaens ME, Arts ICW, Thijssen DHJ, Feskens EJM, Goossens GH, Afman LA, and Blaak EE
- Subjects
- Female, Humans, Diet, Fat-Restricted, Insulin, Phenotype, Adult, Middle Aged, Aged, Cardiovascular Diseases prevention & control, Insulin Resistance physiology
- Abstract
Precision nutrition based on metabolic phenotype may increase the effectiveness of interventions. In this proof-of-concept study, we investigated the effect of modulating dietary macronutrient composition according to muscle insulin-resistant (MIR) or liver insulin-resistant (LIR) phenotypes on cardiometabolic health. Women and men with MIR or LIR (n = 242, body mass index [BMI] 25-40 kg/m
2 , 40-75 years) were randomized to phenotype diet (PhenoDiet) group A or B and followed a 12-week high-monounsaturated fatty acid (HMUFA) diet or low-fat, high-protein, and high-fiber diet (LFHP) (PhenoDiet group A, MIR/HMUFA and LIR/LFHP; PhenoDiet group B, MIR/LFHP and LIR/HMUFA). PhenoDiet group B showed no significant improvements in the primary outcome disposition index, but greater improvements in insulin sensitivity, glucose homeostasis, serum triacylglycerol, and C-reactive protein compared with PhenoDiet group A were observed. We demonstrate that modulating macronutrient composition within the dietary guidelines based on tissue-specific insulin resistance (IR) phenotype enhances cardiometabolic health improvements. Clinicaltrials.gov registration: NCT03708419, CCMO registration NL63768.068.17., Competing Interests: Declaration of interests S.P. is an employee at DSM Nutritional Products, C.M.S.-P. is an employee at FrieslandCampina, and J.d.V.-v.d.B. is an employee at Danone Nutricia Research., (Copyright © 2022 The Author(s). Published by Elsevier Inc. All rights reserved.)- Published
- 2023
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19. Healthy aging and muscle function are positively associated with NAD + abundance in humans.
- Author
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Janssens GE, Grevendonk L, Perez RZ, Schomakers BV, de Vogel-van den Bosch J, Geurts JMW, van Weeghel M, Schrauwen P, Houtkooper RH, and Hoeks J
- Subjects
- Humans, Aged, Cross-Sectional Studies, Aging metabolism, Muscle, Skeletal metabolism, NAD metabolism, Healthy Aging
- Abstract
Skeletal muscle is greatly affected by aging, resulting in a loss of metabolic and physical function. However, the underlying molecular processes and how (lack of) physical activity is involved in age-related metabolic decline in muscle function in humans is largely unknown. Here, we compared, in a cross-sectional study, the muscle metabolome from young to older adults, whereby the older adults were exercise trained, had normal physical activity levels or were physically impaired. Nicotinamide adenine dinucleotide (NAD
+ ) was one of the most prominent metabolites that was lower in older adults, in line with preclinical models. This lower level was even more pronounced in impaired older individuals, and conversely, exercise-trained older individuals had NAD+ levels that were more similar to those found in younger individuals. NAD+ abundance positively correlated with average number of steps per day and mitochondrial and muscle functioning. Our work suggests that a clear association exists between NAD+ and health status in human aging., (© 2022. The Author(s), under exclusive licence to Springer Nature America, Inc.)- Published
- 2022
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20. Effect of an Enriched Protein Drink on Muscle Mass and Glycemic Control during Combined Lifestyle Intervention in Older Adults with Obesity and Type 2 Diabetes: A Double-Blind RCT.
- Author
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Memelink RG, Pasman WJ, Bongers A, Tump A, van Ginkel A, Tromp W, Wopereis S, Verlaan S, de Vogel-van den Bosch J, and Weijs PJM
- Subjects
- Absorptiometry, Photon, Aged, Anthropometry, Body Composition, Double-Blind Method, Exercise, Female, Glucose Tolerance Test, Humans, Insulin Resistance, Leucine, Male, Middle Aged, Muscle Strength, Overweight, Proteins, Vitamin D, Weight Loss, Diabetes Mellitus, Type 2 complications, Dietary Proteins administration & dosage, Glycemic Control methods, Life Style, Muscles, Obesity complications
- Abstract
Background: Weight loss is key to treatment of older adults with obesity and type 2 diabetes, but also a risk for muscle mass loss. This study investigated whether a whey protein drink enriched with leucine and vitamin D could preserve muscle mass and improve glycemic control during combined lifestyle intervention in this population., Methods: 123 older adults with obesity and type 2 diabetes were randomized into a 13-week lifestyle intervention with dietary advice and exercise, receiving either the enriched protein drink (test) or an isocaloric control (control). Muscle mass was assessed with dual-energy X-ray absorptiometry and glycemic control by oral glucose tolerance test. Statistical analyses were performed using a linear mixed model., Results: There was a nonsignificant increase in leg muscle mass (+0.28 kg; 95% CI, -0.01 to 0.56) and a significant increase in appendicular muscle mass (+0.36 kg; 95% CI, 0.005 to 0.71) and total lean mass (+0.92 kg; 95% CI, 0.19 to 1.65) in test vs. control. Insulin sensitivity (Matsuda index) also increased in test vs. control (+0.52; 95% CI, 0.07 to 0.97)., Conclusions: Use of an enriched protein drink during combined lifestyle intervention shows beneficial effects on muscle mass and glycemic control in older adults with obesity and type 2 diabetes.
- Published
- 2020
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21. Effects of Beetroot Powder with or without L-Arginine on Postprandial Vascular Endothelial Function: Results of a Randomized Controlled Trial with Abdominally Obese Men.
- Author
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Smeets ETHC, Mensink RP, Hoeks J, de Vogel-Van den Bosch J, Hageman RJJ, and Joris PJ
- Subjects
- Aged, Blood Flow Velocity drug effects, Blood Glucose, Brachial Artery physiopathology, Cross-Over Studies, Dietary Supplements, Endothelium, Vascular drug effects, Food, Humans, Insulin blood, Male, Middle Aged, Nitric Oxide blood, Postprandial Period, Powders, Triglycerides blood, Vascular Stiffness drug effects, Arginine administration & dosage, Beta vulgaris chemistry, Blood Flow Velocity physiology, Endothelium, Vascular physiopathology, Obesity, Abdominal physiopathology, Vasodilation drug effects
- Abstract
Background: Through effects on nitric oxide bioavailability, vascular endothelial function is improved after the intake of a high amount of nitrate or L-arginine, but decreased after the intake of a high-fat meal. Therefore, we compared the effects of beetroot powder with or without L-arginine on postprandial brachial artery flow-mediated vasodilation (FMD) after consumption of a high-fat mixed-meal., Methods: Eighteen abdominally obese men completed this randomized, double-blinded, cross-over trial. The study consisted of five test days, each separated by a wash-out period of at least one week. Participants received in random order, a blended meal with a control or nutritional supplement consisting of beetroot powder providing 200 mg nitrate, beetroot with 0.8 g of L-arginine, beetroot with 1.5 g of L-arginine, or 3.0 g of L-arginine. Participants then fasted and 2 h postprandial FMD measurements were performed., Results: No significant differences between meals were observed for postprandial FMD ( p = 0.45) levels. However, there was a non-significant trend towards a more beneficial postprandial FMD response with the beetroot-containing meals as compared with meals without beetroot., Conclusion: This trial could not provide evidence for beneficial additive effects of a single dose of beetroot powder combined with L-arginine on postprandial endothelial function in abdominally obese men.
- Published
- 2020
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22. Obese Older Type 2 Diabetes Mellitus Patients with Muscle Insulin Resistance Benefit from an Enriched Protein Drink during Combined Lifestyle Intervention: The PROBE Study.
- Author
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Pasman WJ, Memelink RG, de Vogel-Van den Bosch J, Begieneman MPV, van den Brink WJ, Weijs PJM, and Wopereis S
- Subjects
- Adipose Tissue metabolism, Aged, Blood Glucose analysis, Diabetes Mellitus, Type 2 blood, Female, Glucose Tolerance Test, Glycated Hemoglobin analysis, Humans, Insulin blood, Life Style, Male, Muscle, Skeletal drug effects, Retrospective Studies, Treatment Outcome, Beverages, Diabetes Mellitus, Type 2 therapy, Dietary Proteins administration & dosage, Food, Fortified, Insulin Resistance physiology
- Abstract
(1) Background: Recent research showed that subtypes of patients with type 2 diabetes may differ in response to lifestyle interventions based on their organ-specific insulin resistance (IR). (2) Methods: 123 Subjects with type 2 diabetes were randomized into 13-week lifestyle intervention, receiving either an enriched protein drink (protein+) or an isocaloric control drink (control). Before and after the intervention, anthropometrical and physiological data was collected. An oral glucose tolerance test was used to calculate indices representing organ insulin resistance (muscle, liver, and adipose tissue) and β-cell functioning. In 82 study-compliant subjects (per-protocol), we retrospectively examined the intervention effect in patients with muscle IR (MIR, n = 42) and without MIR (no-MIR, n = 40). (3) Results: Only in patients from the MIR subgroup that received protein
+ drink, fasting plasma glucose and insulin, whole body, liver and adipose IR, and appendicular skeletal muscle mass improved versus control. Lifestyle intervention improved body weight and fat mass in both subgroups. Furthermore, for the MIR subgroup decreased systolic blood pressure and increased VO2 peak and for the no-MIR subgroup, a decreased 2-h glucose concentration was found. (4) Conclusions: Enriched protein drink during combined lifestyle intervention seems to be especially effective on increasing muscle mass and improving insulin resistance in obese older, type 2 diabetes patients with muscle IR.- Published
- 2020
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23. Glycaemic response after intake of a high energy, high protein, diabetes-specific formula in older malnourished or at risk of malnutrition type 2 diabetes patients.
- Author
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Laksir H, Lansink M, Regueme SC, de Vogel-van den Bosch J, Pfeiffer AFH, and Bourdel-Marchasson I
- Subjects
- Aged, Aged, 80 and over, Cross-Over Studies, Double-Blind Method, Female, Humans, Insulin blood, Male, Blood Glucose metabolism, Diabetes Mellitus, Type 2 complications, Diabetes Mellitus, Type 2 diet therapy, Diabetes Mellitus, Type 2 metabolism, Diet, High-Protein, Food, Formulated, Malnutrition complications, Malnutrition metabolism, Malnutrition prevention & control
- Abstract
Background & Aims: Several studies with diabetes-specific formulas (DSFs) for hyperglycaemic patients in need of nutritional support have been conducted in non-malnourished patients, mainly comparing products with varying macronutrient compositions. Here, the effect of a high energy, high protein DSF on postprandial responses was compared to a product with a similar macronutrient composition in malnourished or at risk of malnutrition patients with type 2 diabetes., Methods: In this randomised, double-blind cross-over study, 20 patients were included. After overnight fasting, patients consumed 200 mL of a DSF or standard supplement (control) (19.6 g protein, 31.2 g carbohydrates and 10.6 g fat), while continuing their anti-diabetic medication. The formulas differed in type of carbohydrates and presence of fibre. The postprandial glucose, insulin and glucagon responses were monitored over 4 h. Data were analysed with a Linear Mixed Model, and results of the modified ITT population (n = 19) are shown., Results: Postprandial glucose response as incremental area under the curve (iAUC), was lower after consumption of DSF compared with control (489.7 ± 268.5 (mean ± SD) vs 581.3 ± 273.9 mmol/L min, respectively; p = 0.008). Also, the incremental maximum concentration of glucose (iCmax) was lower for DSF vs control (3.5 ± 1.4 vs 4.0 ± 1.4 mmol/L; p = 0.007). Postprandial insulin and glucagon levels, expressed as iAUC or iCmax, were not significantly different between groups., Conclusions: Consumption of a high energy, high protein DSF by older malnourished or at risk of malnutrition type 2 diabetes patients resulted in a significantly lower glucose response compared to control. These data suggest that the use of a DSF is preferred for patients with diabetes in need of nutritional support., (Copyright © 2017 The Authors. Published by Elsevier Ltd.. All rights reserved.)
- Published
- 2018
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24. Reply to AM Bernstein et al.
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Verreijen AM, de Vogel-van den Bosch J, Verlaan S, and Weijs PJ
- Subjects
- Female, Humans, Male, Dietary Supplements, Leucine administration & dosage, Milk Proteins administration & dosage, Muscle, Skeletal physiology, Obesity therapy, Vitamin D administration & dosage
- Published
- 2015
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25. A high whey protein-, leucine-, and vitamin D-enriched supplement preserves muscle mass during intentional weight loss in obese older adults: a double-blind randomized controlled trial.
- Author
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Verreijen AM, Verlaan S, Engberink MF, Swinkels S, de Vogel-van den Bosch J, and Weijs PJ
- Subjects
- Aged, Aged, 80 and over, Body Composition, Body Mass Index, Body Weight, Diet, Reducing, Dietary Proteins administration & dosage, Double-Blind Method, Energy Intake, Female, Hand Strength, Humans, Male, Middle Aged, Motor Activity, Muscle Strength, Resistance Training, Sarcopenia prevention & control, Treatment Outcome, Weight Loss, Whey Proteins, Dietary Supplements, Leucine administration & dosage, Milk Proteins administration & dosage, Muscle, Skeletal physiology, Obesity therapy, Vitamin D administration & dosage
- Abstract
Background: Intentional weight loss in obese older adults is a risk factor for muscle loss and sarcopenia., Objective: The objective was to examine the effect of a high whey protein-, leucine-, and vitamin D-enriched supplement on muscle mass preservation during intentional weight loss in obese older adults., Design: We included 80 obese older adults in a double-blind randomized controlled trial. During a 13-wk weight loss program, all subjects followed a hypocaloric diet (-600 kcal/d) and performed resistance training 3×/wk. Subjects were randomly allocated to a high whey protein-, leucine-, and vitamin D-enriched supplement including a mix of other macro- and micronutrients (150 kcal, 21 g protein; 10×/wk, intervention group) or an isocaloric control. The primary outcome was change in appendicular muscle mass. The secondary outcomes were body composition, handgrip strength, and physical performance. Data were analyzed by using ANCOVA and mixed linear models with sex and baseline value as covariates., Results: At baseline, mean ± SD age was 63 ± 5.6 y, and body mass index (in kg/m(2)) was 33 ± 4.4. During the trial, protein intake was 1.11 ± 0.28 g · kg body weight(-1) · d(-1) in the intervention group compared with 0.85 ± 0.24 g · kg body weight(-1) · d(-1) in the control group (P < 0.001). Both intervention and control groups decreased in body weight (-3.4 ± 3.6 kg and -2.8 ± 2.8 kg; both P < 0.001) and fat mass (-3.2 ± 3.1 kg and -2.5 ± 2.4 kg; both P < 0.001), with no differences between groups. The 13-wk change in appendicular muscle mass, however, was different in the intervention and control groups [+0.4 ± 1.2 kg and -0.5 ± 2.1 kg, respectively; β = 0.95 kg (95% CI: 0.09, 1.81); P = 0.03]. Muscle strength and function improved over time without significant differences between groups., Conclusion: A high whey protein-, leucine-, and vitamin D-enriched supplement compared with isocaloric control preserves appendicular muscle mass in obese older adults during a hypocaloric diet and resistance exercise program and might therefore reduce the risk of sarcopenia. This trial was registered at the Dutch Trial Register (http://www.trialregister.nl) as NTR2751., (© 2015 American Society for Nutrition.)
- Published
- 2015
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26. Saturated fat stimulates obesity and hepatic steatosis and affects gut microbiota composition by an enhanced overflow of dietary fat to the distal intestine.
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de Wit N, Derrien M, Bosch-Vermeulen H, Oosterink E, Keshtkar S, Duval C, de Vogel-van den Bosch J, Kleerebezem M, Müller M, and van der Meer R
- Subjects
- Animals, Fatty Liver genetics, Gene Expression drug effects, Intestinal Mucosa metabolism, Intestines microbiology, Liver metabolism, Metabolic Syndrome genetics, Metagenome, Mice, Mice, Inbred C57BL, Obesity genetics, Dietary Fats pharmacology, Fatty Acids pharmacology, Fatty Liver metabolism, Intestines drug effects, Metabolic Syndrome metabolism, Obesity metabolism
- Abstract
We studied the effect of dietary fat type, varying in polyunsaturated-to-saturated fatty acid ratios (P/S), on development of metabolic syndrome. C57Bl/6J mice were fed purified high-fat diets (45E% fat) containing palm oil (HF-PO; P/S 0.4), olive oil (HF-OO; P/S 1.1), or safflower oil (HF-SO; P/S 7.8) for 8 wk. A low-fat palm oil diet (LF-PO; 10E% fat) was used as a reference. Additionally, we analyzed diet-induced changes in gut microbiota composition and mucosal gene expression. The HF-PO diet induced a higher body weight gain and liver triglyceride content compared with the HF-OO, HF-SO, or LF-PO diet. In the intestine, the HF-PO diet reduced microbial diversity and increased the Firmicutes-to-Bacteroidetes ratio. Although this fits a typical obesity profile, our data clearly indicate that an overflow of the HF-PO diet to the distal intestine, rather than obesity itself, is the main trigger for these gut microbiota changes. A HF-PO diet-induced elevation of lipid metabolism-related genes in the distal small intestine confirmed the overflow of palm oil to the distal intestine. Some of these lipid metabolism-related genes were previously already associated with the metabolic syndrome. In conclusion, our data indicate that saturated fat (HF-PO) has a more stimulatory effect on weight gain and hepatic lipid accumulation than unsaturated fat (HF-OO and HF-SO). The overflow of fat to the distal intestine on the HF-PO diet induced changes in gut microbiota composition and mucosal gene expression. We speculate that both are directly or indirectly contributive to the saturated fat-induced development of obesity and hepatic steatosis.
- Published
- 2012
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27. The effects of long- or medium-chain fat diets on glucose tolerance and myocellular content of lipid intermediates in rats.
- Author
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De Vogel-van den Bosch J, Hoeks J, Timmers S, Houten SM, van Dijk PJ, Boon W, Van Beurden D, Schaart G, Kersten S, Voshol PJ, Wanders RJ, Hesselink MK, and Schrauwen P
- Subjects
- Analysis of Variance, Animals, Blood Glucose metabolism, Blotting, Western, Body Weight, Carnitine metabolism, Dietary Fats metabolism, Diglycerides analysis, Energy Metabolism, Glucose Tolerance Test, Insulin blood, Insulin Resistance, Lipid Peroxidation, Male, Muscle, Skeletal drug effects, Rats, Rats, Wistar, Carnitine analogs & derivatives, Dietary Fats administration & dosage, Fatty Acids pharmacokinetics, Glucose Intolerance metabolism, Muscle, Skeletal metabolism, Triglycerides pharmacokinetics
- Abstract
Accumulation of triacylglycerols (TAGs) and acylcarnitines in skeletal muscle upon high-fat (HF) feeding is the resultant of fatty acid uptake and oxidation and is associated with insulin resistance. As medium-chain fatty acids (MCFAs) are preferentially β-oxidized over long-chain fatty acids, we examined the effects of medium-chain TAGs (MCTs) and long-chain TAGs (LCTs) on muscle lipid storage and whole-body glucose tolerance. Rats fed a low-fat (LF), HFLCT, or an isocaloric HFMCT diet displayed a similar body weight gain over 8 weeks of treatment. Only HFLCT increased myocellular TAG (42.3 ± 4.9, 71.9 ± 6.7, and 48.5 ± 6.5 µmol/g for LF, HFLCT, and HFMCT, respectively, P < 0.05) and long-chain acylcarnitine content (P < 0.05). Neither HF diet increased myocellular diacylglycerol (DAG) content. Intraperitoneal (IP) glucose tolerance tests (1.5 g/kg) revealed a significantly decreased glucose tolerance in the HFMCT compared to the HFLCT-fed rats (802 ± 40, 772 ± 18, and 886 ± 18 area under the curve for LF, HFLCT, and HFMCT, respectively, P < 0.05). Finally, no differences in myocellular insulin signaling after bolus insulin injection (10 U/kg) were observed between LF, HFLCT, or HFMCT-fed rats. These results show that accumulation of TAGs and acylcarnitines in skeletal muscle in the absence of body weight gain do not impede myocellular insulin signaling or whole-body glucose intolerance.
- Published
- 2011
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28. High-fat diets rich in medium- versus long-chain fatty acids induce distinct patterns of tissue specific insulin resistance.
- Author
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De Vogel-van den Bosch J, van den Berg SA, Bijland S, Voshol PJ, Havekes LM, Romijn HA, Hoeks J, van Beurden D, Hesselink MK, Schrauwen P, and van Dijk KW
- Subjects
- Animals, Dietary Fats pharmacology, Energy Intake, Glucose metabolism, Male, Mice, Mice, Inbred C57BL, Triglycerides administration & dosage, Dietary Fats administration & dosage, Fatty Acids administration & dosage, Insulin Resistance physiology
- Abstract
Excess dietary long-chain fatty acid (LCFA) intake results in ectopic lipid accumulation and insulin resistance. Since medium-chain fatty acids (MCFA) are preferentially oxidized over LCFA, we hypothesized that diets rich in MCFA result in a lower ectopic lipid accumulation and insulin resistance compared to diets rich in LCFA. Feeding mice high-fat (HF) (45% kcal fat) diets for 8 weeks rich in triacylglycerols composed of MCFA (HFMCT) or LCFA (HFLCT) revealed a lower body weight gain in the HFMCT-fed mice. Indirect calorimetry revealed higher fat oxidation on HFMCT compared to HFLCT (0.011.0±0.0007 vs. 0.0096±0.0015 kcal/g body weight per hour, P<.05). In line with this, neutral lipid immunohistochemistry revealed significantly lower lipid storage in skeletal muscle (0.05±0.08 vs. 0.30±0.23 area%, P <.05) and in liver (0.9±0.4 vs. 6.4±0.8 area%, P<.05) after HFMCT vs. HFLCT, while ectopic fat storage in low fat (LF) was very low. Hyperinsulinemic euglycemic clamps revealed that the HFMCT and HFLCT resulted in severe whole body insulin resistance (glucose infusion rate: 53.1±6.8, 50.8±15.3 vs. 124.6±25.4 μmol min(-1) kg(-1), P<.001 in HFMCT, HFLCT and LF-fed mice, respectively). However, under hyperinsulinemic conditions, HFMCT revealed a lower endogenous glucose output (22.6±8.0 vs. 34.7±8.5 μmol min(-1) kg(-1), P<.05) and a lower peripheral glucose disappearance (75.7±7.8 vs. 93.4±12.4 μmol min(-1) kg(-1), P<.03) compared to HFLCT-fed mice. In conclusion, both HF diets induced whole body insulin resistance compared to LF. However, the HFMCT gained less weight, had less ectopic lipid accumulation, while peripheral insulin resistance was more pronounced compared to HFLCT. This suggests that HF-diets rich in medium- versus long-chain triacylglycerols induce insulin resistance via distinct mechanisms., (Copyright © 2011 Elsevier Inc. All rights reserved.)
- Published
- 2011
- Full Text
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29. Paradoxical increase in TAG and DAG content parallel the insulin sensitizing effect of unilateral DGAT1 overexpression in rat skeletal muscle.
- Author
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Timmers S, de Vogel-van den Bosch J, Hesselink MK, van Beurden D, Schaart G, Ferraz MJ, Losen M, Martinez-Martinez P, De Baets MH, Aerts JM, and Schrauwen P
- Subjects
- Animals, Diacylglycerol O-Acyltransferase administration & dosage, Diglycerides metabolism, Electroporation, Gene Expression, Insulin Resistance, Male, Rats, Triglycerides metabolism, Diacylglycerol O-Acyltransferase biosynthesis, Diglycerides analysis, Insulin physiology, Muscle, Skeletal enzymology, Triglycerides analysis
- Abstract
Background: The involvement of muscle triacylglycerol (TAG) storage in the onset of insulin resistance is questioned and the attention has shifted towards inhibition of insulin signalling by the lipid intermediate diacylglycerol (DAG). The enzyme 1,2-acylCoA:diacylglyceroltransferase-1 (DGAT1) esterifies a fatty acyl-CoA on DAG to form TAG. Therefore, the aim of the present study was to investigate if unilateral overexpression of DGAT1 in adult rat Tibialis anterior (TA) muscle will increase conversion of the lipid intermediate DAG into TAG, thereby improving muscle insulin sensitivity., Methodology/principal Findings: The DGAT1 gene construct was injected in the left TA muscle of male rats on chow or high-fat (45% kcal) diet for three weeks, followed by application of one 800 V/cm and four 80 V/cm pulses, using the contralateral leg as sham-electroporated control. Seven days after electroporation, muscle specific insulin sensitivity was assessed with a hyperinsulinemic euglycemic clamp using 2-deoxy-[3H]glucose. Here, we provide evidence that unilateral overexpression of DGAT1 in TA muscle of male rats is associated with an increased rather than decreased DAG content. Strikingly, this increase in DAG content was accompanied by improved muscle insulin sensitivity. Interestingly, markers of muscle lipolysis and mitochondrial function were also increased in DGAT1 overexpressing muscle., Conclusions/significance: We conclude that unilateral DGAT1 overexpression can rescue insulin sensitivity, possibly by increasing DAG and TAG turnover in skeletal muscle. In case of a proper balance between the supply and oxidation of fatty acids in skeletal muscle, the lipid intermediate DAG may not exert harmful effects on insulin signalling.
- Published
- 2011
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30. Prevention of high-fat diet-induced muscular lipid accumulation in rats by alpha lipoic acid is not mediated by AMPK activation.
- Author
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Timmers S, de Vogel-van den Bosch J, Towler MC, Schaart G, Moonen-Kornips E, Mensink RP, Hesselink MK, Hardie DG, and Schrauwen P
- Subjects
- Absorption drug effects, Adult, Animals, Body Composition drug effects, Body Weight drug effects, Dietary Supplements, Eating drug effects, Enzyme Activation drug effects, Glucose metabolism, Glucose Tolerance Test, Humans, Insulin metabolism, Male, Muscle, Skeletal drug effects, Muscle, Skeletal metabolism, Obesity chemically induced, Obesity metabolism, Obesity physiopathology, Obesity prevention & control, Rats, Rats, Wistar, Signal Transduction drug effects, Thioctic Acid administration & dosage, AMP-Activated Protein Kinases metabolism, Dietary Fats pharmacology, Lipid Metabolism drug effects, Muscles drug effects, Muscles metabolism, Thioctic Acid pharmacology
- Abstract
Skeletal muscle triglyceride accumulation is associated with insulin resistance in obesity. Recently, it has been suggested that alpha lipoic acid (ALA) improves insulin sensitivity by lowering triglyceride accumulation in nonadipose tissues via activation of skeletal muscle AMP-activated protein kinase (AMPK). We examined whether chronic ALA supplementation prevents muscular lipid accumulation that is associated with high-fat diets via activation of AMPK. In addition, we tested if ALA supplementation was able to improve insulin sensitivity in rats fed low- and high-fat diets (LFD, HFD). Supplementing male Wistar rats with 0.5% ALA for 8 weeks significantly reduced body weight, both on LFD and HFD (-24% LFD+ALA vs. LFD, P < 0.01, and -29% HFD+ALA vs. HFD, P < 0.001). Oil red O lipid staining revealed a 3-fold higher lipid content in skeletal muscle after HFD compared with LFD and ALA-supplemented groups (P < 0.05). ALA improved whole body glucose tolerance ( approximately 20% lower total area under the curve (AUC) in ALA supplemented groups vs. controls, P < 0.05). These effects were not mediated by increased muscular AMPK activation or ALA-induced improvement of muscular insulin sensitivity. To conclude, the prevention of HFD-induced muscular lipid accumulation and the improved whole body glucose tolerance are likely secondary effects due to the anorexic nature of ALA.
- Published
- 2010
- Full Text
- View/download PDF
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