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Climate change intensifies soil salinization and jeopardizes the development of crops worldwide. The accumulation of salts in plant tissue activates the defense system and triggers ethylene production thus restricting cell division. We hypothesize that the inoculation of plant growth-promoting bacteria (PGPB) producing ACC (1-aminocyclopropane-1-carboxylate) deaminase favors the development of arbuscular mycorrhizal fungi (AMF), promoting the growth of maize plants under saline stress. We investigated the efficacy of individual inoculation of PGPB, which produce ACC deaminase, as well as the co-inoculation of PGPB with Rhizophagus clarus on maize plant growth subjected to saline stress. The isolates were acquired from the bulk and rhizospheric soil of Mimosa bimucronata (DC.) Kuntze in a temporary pond located in Pernambuco State, Brazil. In the first greenhouse experiment, 10 halophilic PGPB were inoculated into maize at 0, 40 and 80 mM of NaCl, and in the second experiment, the PGPB that showed the best performance were co-inoculated with R. clarus in maize under the same conditions as in the first experiment. Individual PGPB inoculation benefited the number of leaves, stem diameter, root and shoot dry mass, and the photosynthetic pigments. Inoculation with PGPB 28-10 Pseudarthrobacter enclensis, 24-1 P. enclensis and 52 P. chlorophenolicus increased the chlorophyll a content by 138%, 171%, and 324% at 0, 40 and 80 mM NaCl, respectively, comparing to the non-inoculated control. We also highlight that the inoculation of PGPB 28-10, 28-7 Arthrobacter sp. and 52 increased the content of chlorophyll b by 72%, 98%, and 280% and carotenoids by 82%, 98%, and 290% at 0, 40 and 80 mM of NaCl, respectively. Co-inoculation with PGPB 28-7, 46-1 Leclercia tamurae, 70 Artrobacter sp., and 79-1 Micrococcus endophyticus significantly increased the rate of mycorrhizal colonization by roughly 50%. Furthermore, co-inoculation promoted a decrease in the accumulation of Na and K extracted from plant tissue, with an increase in salt concentration, from 40 mM to 80 mM, also favoring the establishment and development of R. clarus. In addition, co-inoculation of these PGPB with R. clarus promoted maize growth and increased plant biomass through osmoregulation and protection of the photosynthetic apparatus. The tripartite symbiosis (plant-fungus-bacterium) is likely to reprogram metabolic pathways that improve maize growth and crop yield, suggesting that the AMF-PGPB consortium can minimize damages caused by saline stress., (Copyright © 2024 Elsevier GmbH. All rights reserved.)

Purpose: The current most important form of transmission for Trypanosoma cruzi is the oral route, being responsible for high mortality during the acute phase in infected individuals. Thus, this study aimed to investigate the possibility of infection for this parasite using sugarcane juice in different temperatures employing metacyclic trypomastigotes obtained from xenodiagnosis performed in Swiss mice previously infected with T.cruzi Y strain, and then diluted in sugarcane juice., Methods: For stomach histopathological analysis, 20 mice were infected with metacyclic trypomastigotes diluted in sugarcane juice and euthanized after the 2nd, 4th, 10th, and 15th days after infection. Concurrently, six batches of ten mice were fed using 1.5 mL of the mixture and kept for 12 h at the temperatures of - 80 ºC, - 20 ºC, + 2 ºC, + 28 ºC, + 60 ºC, and + 80 ºC, for later infection verification., Results: Inflammatory infiltrate was found after the 2nd day of infection, and amastigotes nests were present after the 4th, 10th, and 15th day in the margo plicatus stomach region. Viable trypomastigotes were observed in the microtubes kept at - 80 ºC, - 20 ºC, and + 2 ºC, but the animal's infection was observed in the - 80 ºC and + 2 ºC groups. In vitro tests demonstrated the decrease of T. cruzi trypomastigote viability, which was negative after 120 h at -20 ºC and 144 h at + 2 ºC, in contrast to the maintenance of survival after 168 h at - 80 ºC., Conclusion: We observed the ability of survival and infection of T. cruzi packaged at - 80 ºC without the use of preservatives and, therefore, less suitable for storing food., (© 2023. The Author(s) under exclusive licence to Witold Stefański Institute of Parasitology, Polish Academy of Sciences.)

Aim: Using in vitro assay and eukaryotic cell model of Saccharomyces cerevisiae, we investigated the impact of microbial fermentation on the antioxidant activity of phenolic substances., Methods and Results: Caffeic acid phenethyl ester (CAPE) and mangiferin were fermented by lactic acid bacteria (LAB), and the antioxidant activity of the fermented products was compared to that of the pure substances. This comparison was assessed using high-performance liquid chromatography (HPLC), in vitro by 2,2-Diphenyl-1-picrylhydrazyl (DPPH), and in vivo in yeast cells. The wild-type strain (BY4741) and its isogenic mutants in glutathione (Δgsh1), catalase (Δctt1), and superoxide dismutase (Δsod1) were treated with CAPE and mangiferin, fermented or not, and exposed to hydrogen peroxide (H2O2)-induced stress. The antioxidant activity was evaluated by cellular viability, intracellular oxidation, and lipid peroxidation. We expected that fermentation would change the antioxidant activity of phenolic substances. While HPLC analysis revealed changes in the composition of fermented products, significant alterations in antioxidant activity were only observed when using mutant strains. The fermentation of mangiferin increased dependency on GSH compared to the respective pure phenolic substance to resolve H2O2-induced stress. Additionally, CAPE appeared to act as a preconditioning agent, enhancing antioxidant responses, and promoting increased tolerance to H2O2 stress, and this mechanism was maintained after fermentation., Conclusions: This study highlights that fermentation impacts the enzymatic mechanism of oxidative stress resolution, even though differences could not be observed in in vitro assays or in the wild-type strain., (© The Author(s) 2023. Published by Oxford University Press on behalf of Applied Microbiology International.)

Severe bone atrophies are considered a challenge in the rehabilitation process. In clinical situations involving excessive vertical bone deficiency in edentulous mandibles, there is a risk of fracture and frequently the need for an approach that involves highly complex procedures. In this context, simultaneous three-dimensional bone reconstruction associated with rigid fixation is a viable alternative to optimize longevity and avoid failures in these cases. This clinical case report presents a technique for reconstruction of severely atrophic mandibles in an elderly female patient to allow the implant-supported prosthesis protocol. The placement of immediate implants was possible by using an intraoral approach for fixation of a titanium plate followed by guided bone regeneration in association with recombinant human bone morphogenetic protein-2, deproteinized bovine bone mineral, and titanium mesh in a 1-stage surgical procedure. There are no reports in the literature of this approach for treating of severely atrophic mandibles. This association of techniques was shown to be predictable after 3 years of follow-up. Therefore, this protocol provides safe supported-implant prosthesis rehabilitation for patients with severely atrophic mandible.

Background: The literature discussing musculoskeletal diseases of inner northeastern workers is scarce, although 67,559 cases were reported in Brazil between 2007 and 2016., Objective: This study aimed to evaluate the effect of multiple risk factors that influence the symptoms of work-related musculoskeletal disorders (WMSDs) in wrists, elbows, and shoulders in workers from four different economic sectors., Methods: A sample included 420 workers from the inner regions of the Brazilian states of Alagoas and Bahia. The Nordic Musculoskeletal Questionnaire was used to capture pain symptoms on both sides of the body (left and right). Sociodemographic variables, items from the biomechanical exposure and organizational conditional, in addition to other questionnaires (JCQ, COPSOQ II, ERI) were used to assess the characteristics and occupational risks of the respondents. Ordinal logistic regression model was using to identify the relationship between symptoms and factors., Results: This study highlights the psychosocial, biomechanical, occupational, and sociodemographic variables contributed to development of WMSDs. Use of hand-vibrating tool increased the likelihood of symptoms manifesting on the body. On the other hand, high job control and high job insecurity reduced the likelihood of developing symptoms. On the other hand, high job control and job satisfaction reduced the likelihood of developing symptoms. Factors such as age, curved spine, high job insecurity and excessive commitment contributed to the development of WMSDs only on one side of the body., Conclusions: The development of WMSDs is multifactorial. Sociodemographic, occupational, biomechanical, and psychosocial factors may commonly contribute to WMSD manifesting only on one side of the body more than on both sides.

In the development of functional probiotic food, the carrier matrices should be carefully selected and optimized to ensure the highest levels of probiotic survival in the symbiotic food along storage. Because milk and honey food matrices are rich in antioxidant substances, the aim of the research was to evaluate their effect in protecting lactobacilli from reactive oxygen species (ROS) generated by the addition of hydrogen peroxide. Viability assays were performed with and without the addition of H 2 O 2 , in three different matrices: 0.9% peptone saline, 5% honey, or 12% reconstituted skim milk. The milk matrix provided protection for the Lacticaseibacillus paracasei DTA83 and Lacticaseibacillus rhamnosus DTA76. However, this protective effect was not observed in the survival of Lactobacillus acidophilus La 5. Honey solution did not maintain the viability of probiotic microorganisms exposed to hydrogen peroxide and, on the contrary, caused a significant reduction in the population of L. rhamnosus DTA76 (p < 0.001). Lower membrane lipid peroxidation due to H 2 O 2 exposure was observed in L. acidophilus La 5 and L. rhamnosus DTA76, but this marker showed no relation with viability. It was concluded: (i) lactobacilli from the Lacticaseibacillus genus were the ones that benefited most from the lactic environment; (ii) the absence of the protective effect of honey was possibly due to the presence of Fe 2+ which reacts with H 2 O 2 to produce hydroxyl radicals; and (iii) cell viability did not correlate with membrane lipid peroxidation, and it is not a good marker to evaluate this type of damage in cells of different microorganisms.

Reactive oxygen species (ROS) cause cell damage and death. To reverse these effects, cells produce substances such as reduced glutathione (GSH) that serve as substrates for antioxidant enzymes. One way to combat microbial resistance includes nullifying the effect of glutathione in microbial cells, causing them to die from oxidative stress. The compound 2-((5-nitrothiophen-2-yl)methylene)-N-(pyridin-3-yl) hydrazine carbothioamide (L10) is a new thiophene-thiosemicarbazone derivative with promising antifungal activity. The aim of this study was to evaluate its mechanism of action against Candida albicans using assays that evaluate its effects on redox balance. Treatment with L10 promoted significant changes in the minimum inhibitory concentration (MIC) values in ascorbic acid and GSH protection tests, the latter increasing up to 64-fold of the MIC. Using nuclear magnetic resonance, we demonstrated interaction of L10 and GSH. At concentrations of 4.0 and 8.0 μg/mL, significant changes were observed in ROS production and mitochondrial membrane potential. The cell death profile showed characteristics of initial apoptosis at inhibitory concentrations (4.0 μg/mL). Transmission electron microscopy data corroborated these results and indicated signs of apoptosis, damage to plasma and nuclear membranes, and to mitochondria. Taken together, these results suggest a possible mechanism of action for L10 antifungal activity, involving changes in cellular redox balance, ROS production, and apoptosis-compatible cellular changes., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2020 Elsevier B.V. All rights reserved.)

Background: Yellow fever (YF) is a severe, infectious, but non-communicable arboviral hemorrhagic disease. In the last decades, yellow fever virus (YFV) infections have been prevalent in endemic areas in Brazil, affecting human and non-human primate (NHP) populations. Monitoring of NHP infection started in 1999, and reports of epizootic diseases are considered important indicators of viral transmission, particularly in relation to the sylvatic cycle. This study presents the monitoring of YFV by real-time RT-PCR and the epidemiological findings related to the deaths of NHPs in the south-eastern states and in the north-eastern state of Bahia, during the outbreak of YF in Brazil during 2017 and 2018., Methods: A total of 4198 samples from 2099 NHPs from south-eastern and north-eastern Brazilian states were analyzed by real-time reverse transcription polymerase chain reaction (rtRT-PCR)., Results: A total of 4198 samples from 2099 NHPs from south-eastern and north-eastern Brazilian states were collected between 2017 and 2018. The samples were subjected to molecular diagnostics for YFV detection using real-time reverse transcription polymerase chain reaction (rtRT-PCR) techniques. Epizootics were coincident with human YF cases. Furthermore, our results showed that the YF frequency was higher among marmosets (Callithrix sp.) than in previous reports. Viremia in species of the genus Alouatta and Callithrix differed greatly., Discussion: Our results indicate a need for further investigation of the role of Callithrix spp. in the transmission cycles of YFV in Brazil. In particular, YFV transmission was observed in a region where viral circulation has not been recorded for decades and thus vaccination has not been previously recommended., Conclusions: This highlights the need to straighten epizootic surveillance and evaluate the extent of vaccination programmes in Brazil in previously considered "YFV-free" areas of the country.

Osmotic adjustment is a persisting controversy in studies on the effect of salt and water stress in cowpea crops. Our hypothesis is that the osmotic potential determination method interferes with the osmotic adjustment calculation. The objective of this study was to consider the osmotic adjustment comparing results obtained by pressure-volume (P-V) curves and osmometry. The experiment was conducted in a randomized block design, with six salt water concentrations 0, 20, 40, 60, 80, and 100 mmol L -1 of NaCl in a Fluvisol. The osmotic adjustment found through osmometry were lower than those found through P-V curves. The apoplastic water fraction of the cowpea had a dilution effect, denoting overestimation of the osmotic potential by the methodology based on osmometry. This may be the source of the different interpretations of osmotic adjustment in cowpea plants. Thus, osmotic adjustment should be calculated preferably using the osmotic potential determined by method of pressure-volume curves., (Copyright © 2019 Elsevier Masson SAS. All rights reserved.)

Atriplex nummularia is a halophyte widely employed to recover saline soils and was used as a model to evaluate the water potentials in the soil-plant system under drought and salt stresses. Potted plants grown under 70 and 37% of field capacity irrigated with solutions of NaCl and of a mixture of NaCl, KCl, MgCl 2 and CaCl 2 reproducing six electrical conductivity (EC): 0, 5, 10, 20, 30, and 40 dS m -1 . After 100 days, total water (Ψ w, plant ) and osmotic (Ψ o, plant ) potentials at predawn and midday and Ψ o, soil , matric potential (Ψ m, soil ) and Ψ w, soil were determined. The type of ion in the irrigation water did not influence the soil potential, but was altered by EC. The soil Ψ o component was the largest contributor to Ψ w, soil . Atriplex is surviving ECs close to 40 dS m -1 due to the decrease in the Ψ w . The plants reached a Ψ w of approximately -8 MPa. The water potentials determined for different moisture levels, EC levels and salt types showed huge importance for the management of this species in semiarid regions and can be used to recover salt affected soils.

In the present work, twelve N-substituted 2-(5-nitro-thiophene)-thiosemicarbazones derivatives (L1-12) were synthesized, characterized and their in vitro cytotoxic and antifungal activities were evaluated against Candida sp. and Cryptococcus neoformans. The probable mechanisms of action have been investigated by sorbitol and ergosterol assays. Additionally, ultrastructural study by Scanning Electron Microscopy was performed with the L10 compound. All compounds were obtained in good yield and their chemical structures were characterized on basis of their physico-chemical and Nuclear Magnetic Resonance - NMR, Spectrophotometric Absorption in the Infrared - IR and High-resolution Mass Spectrometry - HRMS data. The results showed that all strains were more sensitive to the compound L10 except Candida tropicalis URM 6551. On the other hand, the cytotoxicity assay by incorporation of tritiated thymidine showed moderate cytotoxic activity on L8 of the 50 μg/mLat which had the best MIC-cytotoxicity relationship. Concerning the study of the possible mechanism of action, the compounds were not able to bind to ergosterol in the membrane, do not act by inhibiting the synthesis of fungal cell wall (sorbitol assay). However, the Scanning Electron Microscopy - SEM analysis shows significant morphological changes in shape, size, number of cells and hyphae, and cell wall indicating a possible mechanism of action by inhibition of enzymes related to the ergosterol biosynthesis pathway. Our results demonstrate that N-substituted 2-(5-nitro-thiophene)-thiosemicarbazones derivatives are potential antifungal agents with activity associated with inhibition of enzymes related to biosynthesis of ergosterol., (Copyright © 2017 Elsevier B.V. All rights reserved.)

In the present work, thirty-two hybrid compounds containing cycloalka[b]thiophene and indole moieties (TN5, TN5 1-7, TN6, TN6 1-7, TN7, TN7 1-7, TN8, TN8 1-7) were designed, synthesized and evaluated for their cytotoxic and antileishmanial activity against Leishmania amazonensis promastigotes. More than half of the compounds (18 compounds) exhibited significant antileishmanial activity (IC50 lower than 10.0μg/L), showing better performance than the reference drugs (tri- and penta-valent antimonials). The most active compounds were TN8-7, TN6-1 and TN7 with respective IC50 values of 2.1, 2.3 and 3.2μg/mL. Demonstrating that all of the compounds were less toxic than the reference drugs, even at the highest evaluated concentration (400μg/mL), no compound tested presented human erythrocyte cytotoxicity. Compound TN8-7's effectiveness against a trivalent antimony-resistant culture was demonstrated. It was observed that TN8-7's antileishmanial activity is associated with DNA fragmentation of L. amazonensis promastigotes. Chemometric studies (CPCA, PCA, and PLS) highlight intrinsic solubility/lipophilicity, and compound size and shape as closely related to activity. Our results suggest that hybrid cycloalka[b]thiophene-indole derivatives may be considered as lead compounds for further development of new drugs for the treatment of leishmaniasis., (Copyright © 2016 Elsevier Ltd. All rights reserved.)

This study aims to investigate the behavior of Atriplex nummularia under field conditions, including its growth, periodic cuttings, salt extraction, and soil chemical properties monitored for 16 months. Three treatments were evaluated: soil cultivated with Atriplex pruned at 6 and 12 months after transplanting (MAT); soil cultivated with plants that were harvested only at the end of the experiment (16 MAT); and a control (uncultivated soil) with four replications. Soil samplings were taken at 0, 6, 12, and 16 MAT. The samples were taken at depths of 0-20, 20-40, 40-60, and 60-80 cm. Biometric variables for growth were monitored monthly. The shoot was divided into leaves, thin stems (< or = 3 mm diameter), and thick stems (> 3 mm diameter) to determine its content of Ca, Mg, Na, K, and Cl. We concluded that pruning regime for Atriplex was efficient mainly because it stimulated regrowth of less lignified material (leaves and stems < or = 3 mm). We found that elements extracted by plant tissue can be quantified accurately, making them valuable indicators of the efficiency of the recovery process. The use of the Atriplex is recommended because the the possibility of revegetating areas inhospitable to most species used in conventional farming.

Purpose: To carry out a study of association between socioeconomic and demographic factors and homicides in general population, in the state of Bahia, in 2009., Methods: This is an ecological study. The data were collected from the database of the Information System about Mortality of the Ministry of Health, from the Brazilian Institute of Geography and Statistics and the Institute of Applied Economic Research. The Global Moran index was calculated for the detection of spatial autocorrelation, and the Local Moran index was calculated for the detection of spatial Clusters. The transformation in the variable answer (homicides rates) was performed and it was shaped using the Conditional Autoregressive Model., Results: The data showed spatial autocorrelation. Two clusters of municipalities with high rates of homicides were identified, one located predominantly in the Greater Metropolitan Region of Salvador and the other in the South Region of Bahia, especially Eunápolis and Lauro de Freitas, which had the highest rates. The Average Residents Variables, local GDP and the Percentage of Illiteracy presented an inverse association with homicide rates, and the variables Firjan's municipal development index of work and income. Enrolment in high school and the Average of Bolsa Família were directly associated., Conclusions: The urbanization process, in most cases, not controlled by the State, in most cases, made the cities bigger and with better socioeconomic conditions, attraction centers for people with different socioeconomic levels, increasing the social inequality among the residents of these regions, with parallel increase in homicide rates.

The scope of this paper was to analyze the perceptions of civil police officers regarding their professional identity, the institution they belong to and their opinion concerning the image society has of their performance. The data are derived from a survey focused on their work and health conditions, using a questionnaire, interviews and focus groups including managers, sheriffs and officers. The relative frequency and average return for answers about working conditions, professional fulfillment and life quality improvement after joining the institution were then determined. Qualitative questions were selected about the officers' opinion of how society perceives the civil police and what it is to be a police officer in the area under study using sense interpretation method analysis. The results point to a negative image that the police officers believe society has about them, which reaffirms the bad image associated with the profession. Rejecting this negative identity, they try to distinguish the differences between the civil police and the military police officers. The conclusion is that action is required to improve working conditions and develop channels of dialogue with the community and promote health campaigns.

The paper discusses the relationship between work and psychic suffering among police officers in the State of Rio de Janeiro. A questionnaire was filled out by these professionals in three separate studies: Capital Baixada Fluminense and Interior. The socio-demographic profile, quality of life, health and working conditions were investigated. Psychic suffering was measured by the SRQ-20. A logistic regression model was used to identify factors associated with psychic suffering. The results showed it to be associated with the following variables: level of satisfaction with the ability to react to difficult situations; performing the work for which the officers were trained; suffering some victimization; unit location; problems in the nervous system; and degree of satisfaction with life in general. Those who reported problems in the nervous system and victimization showed an OR of 7.25 and 3.08, respectively. The professionals who considered themselves dissatisfied or very dissatisfied with the ability to react to difficult situations and life in general showed an OR of 10.85 and 6.69, respectively, compared with those who considered themselves satisfied or very satisfied. Professionals from the Baixada showed an increased risk of psychic suffering compared to a reduced risk among those from the Interior.

The consumption of psychoactive substances by civil and military police of the city of Rio de Janeiro was investigated. Data was gathered from two cross-sectional studies on a questionnaire on work and health conditions given to a sample from the two corporations. The results show higher frequencies of regular consumption of tobacco (23.3% by civil police and 19.1% by military police), daily use of alcohol (12% by civil police and 11% by military police) and tranquilizers in the past year (13.3% by civil police and 10.1% by military police). The consumption of marijuana among officers was 0.1% by civil police and 1.1% by military police, and cocaine use among the military police was 1.1%. Alcohol consumption proved to be intense and causes problems at work and in the social and family relationships of these officers. The need for preventive policies for addiction and the possible underestimation of information on illicit substances is emphasized.

Violence-related deaths are a public health issue by virtue of the magnitude of their impact on society and on the health services. A study of the descriptive temporal trend was conducted using the official national information systems of violence-related mortality in Argentina and Brazil for the period from 1990 to 2010. Indicators were created to evaluate information quality by sex, age, and cause of death. The results demonstrate a temporal trend of improvement in the quality of the information systems. This trend is repeated especially when violence-related deaths are analyzed, with a decrease in the number of records for violence-related death of undetermined intent; as well as a high percentage of firearm-related deaths of undetermined intent in Argentina. The analysis of the quality of information systems regarding violence-related deaths makes it possible to detect problems and orient actions in order to obtain better quality information and therefore permit improvement in the creation of preventive public policies.

Mortality, hospitalization and emergency attendance visits for assault in Brazil, from 1996 to 2007 were analyzed. The data sources are the Mortality Information System/SIM, the Hospital Information System/SIH and the Surveillance System of Violence and Injuries/VIVA of the Ministry of Health. It was focused on males in the 15 to 29 year-old age group, and other variables related to the victim, the aggressor and the event. The male/ woman distribution was 11.6 times higher for mortality, 4.5 times for hospitalization and 2.8 times for hospital emergency treatment. In 2007 the rate of 15 to 29 year-old men was 92.8/100,000 inhabitants. The Southeast and Northeast have the highest incidence and prevalence. The conclusion was that the male/female differential rates occurs during adolescence, intensifies in early adulthood, and despite decreasing in intensity, continues until death. Cultural gender models and socio-structural aspects were examined to explain such marked differences.

Suspicion of Brazilian spotted fever (BSF) should occur in endemic regions upon surveillance of the acute febrile icteric hemorrhagic syndrome (AFIHS). However, limitations associated with currently available laboratory tests pose a challenge to early diagnosis, especially in fatal cases. Two real-time PCR (qPCR) protocols were evaluated to diagnose BSF in 110 fatal AFIHS cases, collected in BSF-endemic regions in 2009-2010. Of these, 24 were positive and 86 negative by indirect immunofluorescence (IFA) assay (cut-off IgG and/or IgM ≥ 128). DNA from these samples was used in the qPCR protocols: one to detect Rickettsia spp. (citrate synthase gene) and another to determine spotted fever group (SFG) Rickettsia species (OmpA gene). Of the 24 IFA-positive samples, 5 (21%) were positive for OmpA and 9 (38%) for citrate synthase. In the IFA-negative group (n=86), OmpA and citrate synthase were positive in 23 (27%) and 27 (31%), respectively. These results showed that the 2 qPCR protocols were about twice as sensitive as the IFA test alone (93% concordance). In conclusion, qPCR is a sensitive method for the diagnosis of fatal BSF cases and should be considered for routine surveillance of AFIHS in places like Brazil, where spotted fever-related lethality is high and other endemic diseases like dengue and leptospirosis can mislead diagnosis., (Copyright © 2012 Elsevier GmbH. All rights reserved.)

The influence of variables at different levels of organization and the effect of time on the occurrence of firearm-related homicides (FRH) in Argentina between 1991 and 2006 was analyzed using multilevel analysis. A three-level Poisson regression model was used. The first level corresponded to the distribution of the number of FRH by sex and age group for each administrative region and (four-year) period; the second corresponded to the variation over time in the interior of each administrative region; the third modeled the variation between administrative regions in accordance with the Level of Urbanization, Percentage of Homes with Unsatisfied Basic Needs and the Percentage of Working Adults. There were 15,067 FRH in persons aged 14 and over between 1991 and 2006 in the 493 administrative regions. The risk of death was higher in males and persons of 15 to 29 years of age; ages above that were associated with a lower risk. The influence of age was greater in central-urban zones and between 1999 and 2002 than during other periods. The level of urbanization was the socioeconomic variable most strongly associated with FRH risk. The risk of death from FRH was 1.6 times higher in central-urban zones compared with non-central zones. In both zones, the risk was highest between 1999 and 2002.

This article is a descriptive epidemiological study of deaths by homicide in Latin American countries (Argentina, Brazil, Colombia and Mexico) from 1990 to 2007. Deaths due to external causes and homicides, as codified in the 9th and 10th revisions of the International Classification of Diseases/ICD, were analyzed considering sex, age and manner of assault. The numbers, ratios and adjusted rates for deaths by homicide are presented. A linear regression model was used to ascertain the trend of homicide rates by age group. During the period, 4,086,216 deaths from external causes and 1,432,971 homicides were registered in these countries. Deaths from external causes rose 54.5% in Argentina but fell in the other countries (37% in Mexico, 31.8% in Colombia, and 8.1% in Brazil). The ratio for deaths by homicide for both sexes was 9.1 in Colombia, 4.4 in Brazil and 1.6 in Mexico, using the Argentinian rates as a benchmark. There were differences in the evolution of homicide rates by age and sex in the countries: the rate rose in Brazil and fell in Colombia for all age groups. The need to prioritize young males in public policies related to health care and prevention is stressed, as well as the need for the region to adopt inclusive policies and broaden and consolidate democracy and the rights of inhabitants.

At present, it is recognized that the Internet plays key role in universalization of opportunities in society in which we live. For people with disabilities, the content must be accessible in all websites, but the assistive technologies used must be adequate to the specific needs of people with disabilities. Dosvox is a free system developed in the Universidade Federal do Rio de Janeiro (UFRJ) specially designed for blind people and used by them in the performance of their tasks with the use of computers. Previously, through exploratory research based on the observation of the interaction of blind students with the Web, usability problems were identified in the Dosvox interface and in the Webvox, the Web browser included in the system, and these problems were related to the fact that the interface is designed in accordance with the mental model of these Information Technology professionals. This study consolidate the problems in earlier phases of the research, link the results with usability heuristics of Nielsen and propose several improvements to Dosvox and its development process.

A diagnostic analysis of care was conducted for rehabilitation of victims of accidents and violence in municipal districts of Pernambuco, Brazil, with over 100 thousand inhabitants. A cross-sectional and descriptive study was carried out. The principles of the triangulation method were followed because the study required the input of various informers and used a variety of collection techniques. An interview was conducted with the municipal manager of each district to map out and select the services to be analyzed. Questionnaires were applied to the managers of the services for identification of the unit, characterization of the activities and description of the structure. The results revealed that more than half of the services involved group care, prevention of relapses and neuropsychomotor stimulation. The team numbered 273 health professionals, with physiotherapists being the largest group (34.8%), followed by psychologists (13.5%), and speech therapists (12.8%). Only one municipal district supplied ortheses, prostheses and auxiliary equipment for locomotion. Deficits of some types of services and discrepancies in the distribution of such services were observed in the different areas of the state.

It is essential that access to information must to be universal given the importance of having a truly global Internet. This study proposes to evaluate a government website with two semiautomatic accessibility evaluation tools. The results have demonstrated that the use of more than one semi-automatic assessment tool can provide enhanced results. The differences in results and presentation in the reports generated by the two tools indicate that there is more complete results by combining tests with different accessibility evaluation tools.

Objectives: To estimate the prevalence of antibodies to hepatitis A virus (antiVHA-IgG) in children aged 7-14 years from public and private schools, and to identify demographic, socioeconomic and sanitation factors associated with the prevalence of antiVHA-IgG., Methods: Seroepidemiological study for the detection of IgG antiVHA. It was conducted from April 2002 to April 2004 with 462 elementary school students from São Luis, ranging from 7 to 14-years of age. Thirty schools randomly selected took part in the study, with a probability proportional to the number of students enrolled; 23 schools were public and 7 were private. Data was obtained through a structured questionnaire. In order to identify the variables independently associated with IgG antiVHA, the Poisson multiple regression analysis was performed, estimating the adjusted PRs and their 95% confidence intervals. The variables associated with the prevalence of hepatitis A with p < 0 were the only ones that remained in the final model. The level of significance of 0.05 (α = 0.05) was adopted., Results: The prevalence of antiVHA-IgG was 64%, 71.5% in public and 36.5% in private schools. After multivariate analysis, age 11 to 14 years, more than one person per bedroom, and less than two bathrooms per household were associated with a higher prevalence of antiVHA-IgG. Higher parental education was associated with lower prevalence of antiVHA-IgG., Conclusions: Hepatitis A is endemic among schoolchildren in São Luís. The prevalence rate was similar to those found in other regions of the country with similar socio-economic and health conditions. Factors historically associated with a higher prevalence of hepatitis A were also identified in this population.

Although there are signs of decline, homicides and traffic-related injuries and deaths in Brazil account for almost two-thirds of all deaths from external causes. In 2007, the homicide rate was 26·8 per 100,000 people and traffic-related mortality was 23·5 per 100,000. Domestic violence might not lead to as many deaths, but its share of violence-related morbidity is large. These are important public health problems that lead to enormous individual and collective costs. Young, black, and poor men are the main victims and perpetrators of community violence, whereas poor black women and children are the main victims of domestic violence. Regional differentials are also substantial. Besides the sociocultural determinants, much of the violence in Brazil has been associated with the misuse of alcohol and illicit drugs, and the wide availability of firearms. The high traffic-related morbidity and mortality in Brazil have been linked to the chosen model for the transport system that has given priority to roads and private-car use without offering adequate infrastructure. The system is often poorly equipped to deal with violations of traffic rules. In response to the major problems of violence and injuries, Brazil has greatly advanced in terms of legislation and action plans. The main challenge is to assess these advances to identify, extend, integrate, and continue the successful ones., (Copyright © 2011 Elsevier Ltd. All rights reserved.)

Objective: To describe homicide mortality in the municipality of São Paulo according to type of weapon, sex, race or skin color, age, and areas of socioeconomic inequalities, between 1996 and 2008., Method: For this ecological time-series study, data about deaths in the municipality of São Paulo were collected from the municipal program for improvement of mortality information, using International Classification of Diseases, 10th revision (ICD-10) codes. Homicide mortality rates (HMR) were calculated for the overall population and specifically for each sex, race or skin color, age range, type of weapon, and occurrence in social deprivation/affluence areas. HMR were adjusted for age using the direct method. The percentage age of variation in HMR was calculated for the study period. For areas of socioeconomic inequalities, the relative risk of death from homicide was calculated., Results: HMR fell 73.7% between 2001 and 2008. A reduction in HMR was observed in all groups, especially males (-74.5%), young men between 15 and 24 years of age (-78.0%), and residents in areas of extreme socioeconomic deprivation (-79.3%). The reduction occurred mostly in firearm homicide rates (-74.1%). The relative risk of death from homicide in areas of extreme socioeconomic deprivation, as compared to areas with some degree of socioeconomic deprivation, was 2.77 in 1996, 3.9 in 2001, and 2.13 in 2008. In areas of high socioeconomic deprivation, the relative risk was 2.07 in 1996 and 1.96 in 2008., Conclusions: To understand the reduction in homicide rates in the municipality of São Paulo, it is important to take into consideration macrodeterminants that affect the entire municipality and all population subgroups, as well as micro/local determinants that have special impact on homicides committed with firearms and on subgroups such as the young, males, and residents of areas of high socioeconomic deprivation.

This is a research on the aspects of mental health care offered to elderly victims of violence in the cities of Curitiba, Rio de Janeiro, Brasília, Recife and Manaus. It was used the triangulation method that relied on the analysis of secondary data, information from questionnaires and individual and group interviews, defining itself as a target audience of this study, professionals and managers in mental health care. It was investigated the line of practices developed in services regarding policies governing mental health attention and addressed to the elderly who are victims of violence. Among the findings are: the disparity in records between elderly care hospitals (22.3%) and outpatient (0.4%), lack of information on the care of elderly victims of violence; and the incipient perception of professionals regarding the violence as a health intervention. In conclusion, there is a need for greater public investments: in improving the quality of information; the adequacy of the physical structure of services; the training/qualification of professionals to attend for specific elder health issues; under the aspect of violence, it was observed the need to adopt actions of prevention and attention of the most frequent disorders in the elderly.

A situational diagnosis of the health services regarding the care of aged victims of accidents and violence (AVAV) was carried out in Recife, Pernambuco, Brazil. The National Policy for Reducing Accident and Violence Related Morbidity and Mortality and the National Policy for the Aged People Health were used as references. The methodology was based on the triangulation method, with both quantitative and qualitative approaches. Questionnaires and interviews were answered by managers and health staff of hospital, prehospital and rehabilitation services; and local aged health policy managers. In 2006, only the Family Health Program reported prehospital care for AVAV, 31 cases were due to violence and 18 to accidents. The hospital care for aged people was 7.2% of the total care, 27% from accidents and 10% from violence. In the same year, there was no record of rehabilitation care of AVAV. The directives of the policies studied are only partially followed. The health care is deficient in several aspects, such as: clinical protocols; notification devices; support to the aged, caregivers and aggressors; and also continuous training. This analysis can be such a contribution to the reorganization of the local health system, recognizing the aged person as vulnerable to accidents and violence.

This article approaches the insertion of violence against elderly in the Brazilian health care public policies. It presents concepts of violence applied to this group of population, mortality data and number of elder interned by violence and accidents. Also, it compares and discusses some policies that are, direct or indirectly, about elder care, victims of violence, and the specific object of the text. Methodologically, it first aimed to identify if the theme of violence was contemplated in official documents, and if so, which are the guidelines that orient elder care, not only regarding attendance, but also regarding prevention actions. The conclusions are that official policies, with special emphasis to the Elder Statute and the National Policy to Elder Care, are very slowly incorporating this theme and offering support to the Protection Network, mainly in this first decade of the 21st century.

This article reviews the characteristics and operation in the daily routine of a pre-hospital care to the elderly victims of violence and accidents in five capitals (Manaus, Recife, Brasília, Rio de Janeiro and Curitiba). Besides that, it analyses their abilities, and potential obstacles. It was based on the triangulation of quantitative and qualitative methods analyzing data from 80 departments of mobile and fixed pre-hospitals: 32 in Manaus, 18 in Recife, 10 in Brasilia, 12 in Rio de Janeiro and eight in Curitiba. Among the findings are the differences in size and diversity of occupational categories and difficulties of the teams to identify, serve and report cases for lack of training, limited preventive action, almost no involvement and guidance to the family, practically no attention to the perpetrator; incipient articulation and partnership network, especially regarding on pre-hospital services and the hospital.

The follow article presents the methodology used to construct indicators to assess the implementation of the National Policy of Mortality Reduction by Accidents and Violence, of public health policies aimed at the elderly and the Mental Health Policy developed in the research entitled Diagnostic Analysis of Local Health Systems to Meet the Problems Caused by Accidents and Violence against Elderly. These indicators were applied in health services that meet elderly victims of accidents and violence in five Brazilian cities: Brasília, Curitiba, Manaus, Recife and Rio de Janeiro. It started with 124 indicatives to assistance level pre-hospital, hospital, rehabilitation and CAPS. There was a selection phase where indicatives without discriminatory capability were eliminated. It was also decided by the relaxation of some criteria are creating new categories. After this step, a group of the experts validate the questionnaires created with these indicators by using Nominal Technical Group. We performed the Kruskal-Wallis test and a graphical analysis. In the final round, the indicators were grouped by similarity, building synthetic indices, 60 indicatives left. These methods can be used in other organizations to evaluate and adjust their health care based on public policies.

The aim of this article is to describe the public service of Manaus to elderly, either victims of accidents or violence, on three levels of service (32 pre-hospital, 5 hospital and 5 rehabilitation). It is a descriptive study with a quantitative and qualitative approach, where 42 health units were investigated. The results reveal that the emergency services department presents a better physical structure. The organization concerning these matters is still incipient in Manaus. It was detected scarcity in the number of hospital beds and vacancies in the intensive care unit to offer the continuity of the treatment. There's a need for professional qualifications and medical specialties to attend elderly admitted due to injury. Very little has been done in the prevention area. Data entry and notification appear as one of the weakest points on the three levels of attention. The most critical point is related to hospitalization, being this level of attention largely mentioned by managers and health professionals as in great need for re-structuring.

This article presents the vision of managers and health professionals about the care of elderly victims of violence in Rio de Janeiro, showing the specificities of this attention in pre-hospital, hospital and rehabilitation areas, including mental health services. Based on a qualitative methodology, 20 semi-structured interviews with managers and health professionals were produced. The analysis considered the perceptions about violence against elderly, and the specificities of attention provided by SUS services to elderly victims. The State, the family and the social support organizations emerged as structuring categories of the network of care for elderly victims of violence. All attention areas presented insufficient care services or specific programs for the elderly victims of accidents and violence. Also there was a precarious network of social support for care of dependent elderly. With few exceptions elderly are treated as anyone else in public health services, without the priority set by the Statute of the Elderly. The family is called to participate in the care for the elderly in all areas of care. Abandonment, neglect, physical and psychological abuse against seniors cases were reported.

This article presents a review about violence against the elderly, covering the period of 2000 to 2009. The database used in this research was the collection of articles, books, book chapters, manuals and plans of action of the Virtual Library on Violence and Health. We analyzed 115 documents divided into the following categories: falls; external causes and violence in general; epidemiological and socio-epidemiological studies; prevention of violence; violence and accidents in the family; conceptual and methodological review; legal order and denunciation; violence from the elderly's point of view; health services, professionals and caretakers; and construction and validation of research instruments. The results show a relevant increase in production and methodological improvement in public health, social work, law, physiotherapy, nursing, psychology and otorhinolaryngology. However, there are issues that have not been sufficiently approached such as traffic accidents, homicides, suicides, drowning and suffocation.

The increase of elderly population and more active life allow a greater exposure to accidents and violence in this population. A diagnostic analysis of hospital services for emergency and urgent care to victims of accidents and violence in the elderly population was carried out in five capitals of Brazil. The research was based on the principles of triangulation of methods, including quantitative approaches, through the application of questionnaires to managers and pre-hospital, hospital and rehabilitation service professionals, as well as qualitative, in which interviews were made with managers and professionals and with those in charge of elderly health. Based on the guidelines of the policies studied, it was realized that none of the capitals has met all requirements, with a poor attendance characterized by lack of structure to keep a companion for the elderly, referrals to reference services, specific clinical protocols, reporting sheets, support to the elderly, job training and definition of the flow for this population. The outcome showed that the selected health services do not have the appropriate and integral profile to look after the elderly population, demonstrating the need for adequacy of these services aiming the accomplishment of the guidelines of the policies reviewed.

Background: HIV circulating recombinant forms (CRFs) play an important role in the global and regional HIV epidemics, particularly in regions where multiple subtypes are circulating. To date, several (>40) CRFs are recognized worldwide with five currently circulating in Brazil. Here, we report the characterization of near full-length genome sequences (NFLG) of six phylogenetically related HIV-1 BF1 intersubtype recombinants (five from this study and one from other published sequences) representing CRF46&#95;BF1., Methods: Initially, we selected 36 samples from 888 adult patients residing in São Paulo who had previously been diagnosed as being infected with subclade F1 based on pol subgenomic fragment sequencing. Proviral DNA integrated in peripheral blood mononuclear cells (PBMC) was amplified from the purified genomic DNA of all 36-blood samples by five overlapping PCR fragments followed by direct sequencing. Sequence data were obtained from the five fragments that showed identical genomic structure and phylogenetic trees were constructed and compared with previously published sequences. Genuine subclade F1 sequences and any other sequences that exhibited unique mosaic structures were omitted from further analysis, Results: Of the 36 samples analyzed, only six sequences, inferred from the pol region as subclade F1, displayed BF1 identical mosaic genomes with a single intersubtype breakpoint identified at the nef-U3 overlap (HXB2 position 9347-9365; LTR region). Five of these isolates formed a rigid cluster in phylogentic trees from different subclade F1 fragment regions, which we can now designate as CRF46&#95;BF1. According to our estimate, the new CRF accounts for 0.56% of the HIV-1 circulating strains in São Paulo. Comparison with previously published sequences revealed an additional five isolates that share an identical mosaic structure with those reported in our study. Despite sharing a similar recombinant structure, only one sequence appeared to originate from the same CRF46&#95;BF1 ancestor., Conclusion: We identified a new circulating recombinant form with a single intersubtype breakpoint identified at the nef-LTR U3 overlap and designated CRF46&#95;BF1. Given the biological importance of the LTR U3 region, intersubtype recombination in this region could play an important role in HIV evolution with critical consequences for the development of efficient genetic vaccines.

Background and Objective: Periodontitis is a chronic inflammatory disease, and genetic factors may have an important role in its severity. Polymorphisms in the promoter regions of the interleukin-6 (IL-6) and tumour necrosis factor-alpha (TNF-alpha) genes have been reported to cause changes in the production of these cytokines. The aim of this study was to evaluate the possible role of IL-6 (G-174C) and tumour necrosis factor (G-308A) polymorphisms, in the severity of chronic periodontitis in an elderly population., Materials and Methods: In this study, a group of 65 elderly women, comprising 17 patients with moderate chronic periodontitis, 21 with severe chronic periodontitis and 27 healthy patients were selected. DNA was isolated from all subjects, and polymerase chain reaction was used to study the IL-6 and TNF-alpha gene polymorphisms., Results: The results of this study showed a significant difference in the allele and genotype frequencies of IL-6 gene polymorphism between patients with periodontal disease and controls. Subjects carrying the G/G genotype of IL-6 were most severely affected by periodontitis. The TNF-alpha gene polymorphism showed no association with chronic periodontitis between patients and controls., Conclusion: The results suggest that the IL-6 gene polymorphism may be associated with chronic periodontitis, and that TNF-alpha gene polymorphism may not be involved in the progression of chronic periodontitis in the population of elderly Brazilian women.

This paper aims to analyze the rehabilitation services in Recife, Brazil, confronting it with what the National Policy for Decreasing Morbimortality from Accidents and Violence (NPDMAV) professes. Six units of rehabilitation services were analyzed; five were from municipal administration and the other was an ONG on an agreement with SUS. A partial structured interview was conducted with the person in charge for the Attention to the Disable Person's Health aiming to map the network. Also a questionnaire was applied to the managers of the six services aiming to identify the unit and its activities, structure and organization. The Rehabilitation Assistance network in Recife do not meet the NPDMAV guidelines as it presents: decreased number of services/programs; lack of a multidisciplinary team; lack of technological support; deficient joint intra and inter sectors; little interaction between the victim and his family to reinsertion into family and social life; and still incipient prevention and promotion actions. In conclusion, there is an effort to adequate the actions to this population group; however there are relevant covering deficits of people, equipments, information and articulation registers among the various levels of this health network.

This article identifies the conceptions and suggestions of professors of medicine and nursing courses about the insertion of this subject in the formation of their students. An exploratory research was carried out using a questionnaire applied to coordinators of disciplines in such courses at public and private universities in the cities of Rio de Janeiro and Cuiabá. Many of them recognizes violence as a health problem, although 40.7% of medicine professors and 71.1% of nursing professors of the two cities discusses this subject in their classes. The medicine professors suggests the insertion of the violence subject in their students formation with visits to institutions, meeting groups with people in violence situations and interviews with experienced professionals of the area. The nursing professors prefer resources such as films and videos, seminaries and conferences. Gaps were also identified in the students and professors formation, which do not feel able to discuss this thematic. It is indicated that a renovation in the curriculum is urgently necessary.

Background: The genetic diversity of the human immunodeficiency virus type 1 (HIV-1) is critical to lay the groundwork for the design of successful drugs or vaccine. In this study we aimed to characterize and define the molecular prevalence of HIV-1 subclade F1 currently circulating in São Paulo, Brazil., Methods: A total of 36 samples were selected from 888 adult patients residing in São Paulo who had previously been diagnosed in two independent studies in our laboratory as being infected with subclade F1 based on pol subgenomic fragment sequencing. Proviral DNA was amplified from the purified genomic DNA of all 36 blood samples by 5 fragments overlapping PCR followed by direct sequencing. Sequence data were obtained from the 5 fragments of pure subclade F1 and phylogenetic trees were constructed and compared with previously published sequences. Subclades F1 that exhibited mosaic structure with other subtypes were omitted from any further analysis, Results: Our methods of fragment amplification and sequencing confirmed that only 5 sequences inferred from pol region as subclade F1 also holds true for the genome as a whole and, thus, estimated the true prevalence at 0.56%. The results also showed a single phylogenetic cluster of the Brazilian subclade F1 along with non-Brazilian South American isolates in both subgenomic and the full-length genomes analysis with an overall intrasubtype nucleotide divergence of 6.9%. The nucleotide differences within the South American and Central African F1 strains, in the C2-C3 env, were 8.5% and 12.3%, respectively., Conclusion: All together, our findings showed a surprisingly low prevalence rate of subclade F1 in Brazil and suggest that these isolates originated in Central Africa and subsequently introduced to South America.

This study is a descriptive epidemiological analysis of morbidity and mortality from suicide in people aged 10 to 19 years living in the state of Minas Gerais. The source of the mortality data were the data available in the Mortality Information System, DATASUS of the Ministry of Health, over the period 1980-2002. The morbidity data were collected from the Hospital Information System, over the period 1998-2003. Absolute and relative simple frequencies of 2.338 hospitalizations and 1.212 deaths from attempted suicide and suicide over the chosen period were analyzed according to sex, age and method used. The population data used for calculating rates was extracted from the census conducted by the Brazilian Institute of Geography and Statistics. The International Classification of Diseases - ICD 9, from the years 1980 to 1995 and the 10th review since 1996, codes E950 to E959 and X60 to X84, were adopted respectively. The highest hospitalization rates were found among women. Self-poisoning was the most common method for attempting suicide in both sexes. However, adolescent males had the highest mortality rates and the most common methods of suicide (hanging and use of firearms) were more lethal than the method commonly chosen by females (self-poisoning). Based on these findings, the authors emphasize the need for preventing these events among young people.