1. Prepubertal acrylamide exposure causes dose-response decreases in spermatic production and functionality with modulation of genes involved in the spermatogenesis in rats.
- Author
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Ivanski F, de Oliveira VM, de Oliveira IM, de Araújo Ramos AT, de Oliveira Tonete ST, de Oliveira Hykavei G, Bargi-Souza P, Schiessel DL, Martino-Andrade AJ, Romano MA, and Marino Romano R
- Subjects
- Age Factors, Animals, Cation Transport Proteins genetics, Cation Transport Proteins metabolism, Dose-Response Relationship, Drug, Early Growth Response Protein 2 genetics, Early Growth Response Protein 2 metabolism, Estrogen Receptor beta genetics, Estrogen Receptor beta metabolism, Infertility, Male metabolism, Infertility, Male pathology, Infertility, Male physiopathology, Male, Membrane Glycoproteins genetics, Membrane Glycoproteins metabolism, Rats, Wistar, Receptors, Androgen genetics, Receptors, Androgen metabolism, Repressor Proteins genetics, Repressor Proteins metabolism, Risk Assessment, Spermatozoa metabolism, Spermatozoa pathology, Tumor Necrosis Factor-alpha genetics, Tumor Necrosis Factor-alpha metabolism, Acrylamide toxicity, Endocrine Disruptors toxicity, Food Contamination, Infertility, Male chemically induced, Sexual Development drug effects, Spermatogenesis drug effects, Spermatozoa drug effects
- Abstract
The increase in human infertility prevalence due to male reproductive disorders has been associated with extensive endocrine-disrupting chemical (EDC) exposure. Acrylamide (AA) is a compound formed spontaneously during heat processing of some foods that are mainly consumed by children and adolescents. In this study, we evaluated the prepubertal AA exposure effects on male adult reproductive physiology using a prepubertal experimental model to analyze the pubertal development, spermatogenesis hormones levels and genes expression involved in male reproductive function. This study is the first one to use the validated protocol to correlate the AA exposure with puberty development, as well as the AA-induced endocrine disrupting effects on reproductive axis. AA did not affect the age at puberty, the reproductive organ's weight and serum hormonal levels. AA reduces spermatogenesis, induces morphological and functional defects on sperm and alters transcript expression of sexual hormone receptors (Ar and Esr2), the transcript expression of Tnf, Egr2, Rhcg and Lrrc34. These findings suggest that excessive AA consumption may impair their reproductive capacity at adulthood, despite no changes in hormonal profile being observed., Competing Interests: Declaration of Competing Interest All authors declare that they have no conflicts of interest., (Copyright © 2020 Elsevier B.V. All rights reserved.)
- Published
- 2020
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