1. Subacute Effects of Moderate-Intensity Aerobic Exercise in the Fasted State on Cell Metabolism and Signaling in Sedentary Rats.
- Author
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Ayres LR, Vogt ÉL, Schroeder HT, Russo MKB, Von Dentz MC, Rocha DS, Model JFA, Kowalewski LS, de Souza SK, de Oliveira Girelli V, da Rosa Coelho J, de Souza Vargas N, Reischak-Oliveira A, de Bittencourt PIH Jr, Wilhelm EN, Vinagre AS, and Krause M
- Subjects
- Animals, Male, Sirtuin 1 metabolism, Rats, Wistar, Sedentary Behavior, Glycogen metabolism, Rats, Cholesterol blood, Cholesterol metabolism, Peroxisome Proliferator-Activated Receptor Gamma Coactivator 1-alpha metabolism, Uncoupling Protein 1 metabolism, Energy Metabolism physiology, HSP70 Heat-Shock Proteins metabolism, Insulin Resistance physiology, Fasting, Physical Conditioning, Animal physiology, Signal Transduction, Liver metabolism, Adipose Tissue, Brown metabolism, Triglycerides blood, Muscle, Skeletal metabolism
- Abstract
Background: Physical inactivity induces insulin resistance (IR) and metabolic imbalances before any significant changes in adiposity. Recent studies suggest that the beneficial effects of exercise can be potentiated if performed while fasting. This work aimed to compare the subacute effects of fed- and fasted-state single-bout exercise on biochemical parameters and cellular signaling in the metabolism. Methods: The animals were allocated into fed rest (FER), fasting rest (FAR), fed exercise (FEE), and fasting exercise (FAE) groups. The exercise protocol was a 30 min treadmill session at 60% of V˙O
2max . The fasting groups fasted for 8 h before exercise and were killed after 12 h post-exercise. Results: Soleus glycogen concentration increased only in the fasting groups, whereas the triglyceride (TGL) content increased in brown adipose tissue (BAT) and liver in the FAE. The FAE showed decreased plasma total cholesterol concentration compared withthe FAR group. Immunocontent of HSP70, SIRT1, UCP-1, and PGC1-α did not change in any tissue investigated. Conclusions: Our results indicate that physical exercise while fasting can have beneficial metabolic effects on sedentary animals. Remarkably, in the FAE group, there was a reduction in total plasma cholesterol and an increase in the capacity of BAT to metabolize and store nutrients in the form of TGLs.- Published
- 2024
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