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26 results on '"de Oliveira, Sandra Helena Penha"'

19. Influence of Preparation and Exposure Periods of Eluates from Ocular Prosthesis Acrylic Resin in Human Conjunctival Cell Line

Author

da Silva, Emily Vivianne Freitas, dos Santos, Daniela Micheline, Bonatto, Liliane da Rocha, Brito, Victor Gustavo Balera, de Oliveira, Sandra Helena Penha, and Goiato, Marcelo Coelho

Subjects
Collagen Type IV, Eye, Artificial, Interleukin-6, Tumor Necrosis Factor-alpha, Full Length, Acrylic Resins, Methylmethacrylate, Eye, Cell Line, Matrix Metalloproteinase 9, Transforming Growth Factor beta, Humans, RNA, Messenger, Conjunctiva, Materials testing, Cell Proliferation
Abstract

Background: This study was undertaken to analyze if different preparation and exposure periods of eluates from ocular prosthesis acrylic resin influence the cytotoxicity for conjunctival cells. Methods: Twenty-four acrylic resin specimens were divided, according to the period of eluate exposure to Chang conjunctival cells (24 and 72 hours). Eluates were prepared in four different ways: 24, 48, and 72 hours of resin specimen immersion in medium and 24 hours of immersion in water, followed by 24 hours of immersion in medium. MTT assay was used to evaluate the cytotoxic effect. The production of IL-1β, IL-6, TNF-α, and chemokine macrophage inflammatory protein 1α was evaluated by ELISA, while the mRNA expression of type IV collagen (COL IV), transforming growth factor β (TGF-β), and matrix metalloproteinase 9 (MMP9) were evaluated by real-time RT-PCR technique. The statistical analysis was carried out using ANOVA with Bonferroni post-hoc test and the student’s t-test (p < 0.05). Results: Significant quantities of IL-6 (4.594 pg/mL) and mRNA expression of COL IV (1.58) were verified at 72 hours of eluate exposure to cells, as compared to 24 hours. After the 72-hour exposure of eluates to cells, lower cell proliferation (88.4%) and higher IL-6 quantities (12.374 pg/mL), as well as mRNA expression of COL IV (2.21), TGF-β (2.02), and MMP9 (5.75) were observed, which corresponded to 72 hours of a specimen immersed in medium. Conclusion: Longer periods of eluate preparation and exposure from the acrylic resin to cells are related to higher production of proinflammatory cytokines and extracellular matrix proteins.

Published
2019

21. Biological assessment of a new ready-to-use hydraulic sealer

Author

Benetti, Francine, primary, Gomes-Filho, João Eduardo, additional, de Azevedo-Queiroz, India Olinta, additional, Carminatti, Marina, additional, Conti, Letícia Citelli, additional, dos Reis-Prado, Alexandre Henrique, additional, de Oliveira, Sandra Helena Penha, additional, Ervolino, Edilson, additional, Dezan-Júnior, Elói, additional, and Cintra, Luciano Tavares Angelo, additional

Published
2021
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23. Endodontic infections increase leukocyte and lymphocyte levels in the blood

Author

Samuel, Renata Oliveira, primary, Gomes-Filho, João Eduardo, additional, Azuma, Mariane Maffei, additional, Sumida, Dóris Hissako, additional, de Oliveira, Sandra Helena Penha, additional, Chiba, Fernando Yamamoto, additional, Bomfim, Suely Regina Mogami, additional, Ciarlini, Paulo César, additional, Narciso, Luis Gustavo, additional, and Cintra, Luciano Tavares Angelo, additional

Published
2017
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24. Effect of maternal periodontitis on GLUT4 and inflammatory pathway in adult offspring.

Author

Mattera, Maria Sara de Lima Coutinho, Chiba, Fernando Yamamoto, Lopes, Flávia Lombardi, Tsosura, Thaís Verônica Saori, Peres, Maria Angelica, Brito, Victor Gustavo Balera, Oliveira, Sandra Helena Penha, Pereira, Renato Felipe, Marani, Fernando, dos Santos, Rodrigo Martins, Belardi, Bianca Elvira, Tessarin, Gestter Willian Lattari, Benites, Mariana Lopes, Ervolino, Edilson, Heimann, Joel Claudio, Sumida, Doris Hissako, and de Oliveira, Sandra Helena Penha

Abstract

Background: Maternal periodontal disease leads to low birth weight (LBW), insulin resistance (IR), increased TNF-α levels, and alterations in insulin signaling in adult offspring. TNF-α has been associated with the stimulation of IKKβ/NF-κB, resulting in the decreased expression of GLUT4. Another mechanism that may be involved in decreasing GLUT4 expression is DNA methylation. This study aimed to evaluate in the adult offspring of rats with periodontal disease: IR, inflammatory pathways, DNA methylation, and expression of GLUT4.Methods: Female Wistar rats were distributed into control and experimental periodontal disease groups. Seven days after induction of periodontal disease, both groups were mated with healthy male rats. After weaning, male offspring were distributed into control offspring (CN-o) and periodontal disease offspring (PED-o) groups. Body weights were measured from 0-75 days of age. At day 75, the following were measured in the offspring: IR (HOMA-IR index); TNF-α and NF-κBp65 content in the gastrocnemius muscle (GM) by western blotting; IKKα/β, JNK, ERK 1/2, NF-κBp65, and NF-κBp50 phosphorylation status in the GM by western blotting; DNA methylation by restriction digest and real-time PCR(qAMP); and expression of GLUT4 mRNA in the GM by real-time PCR.Results: LBW, IR, increases in TNF-α, IKKα/β, ERK 1/2, NF-κBp65, and NF-κBp50 decreased expression of GLUT4 mRNA were observed in the PED-o rats. No differences were identified in JNK phosphorylation status and DNA methylation in the evaluated regions of the GLUT4-encoding gene Slc2a4.Conclusion: Maternal periodontal disease causes LBW, IR, activation of inflammatory pathways, and decreased GLUT4 expression in the GM of adult offspring. [ABSTRACT FROM AUTHOR]

Published
2019
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25. MTA-induced neutrophil recruitment: a mechanism dependent on IL-1β, MIP-2, and LTB4.

Author

Gomes, Alessandra Cristina, Filho, João Eduardo Gomes, and de Oliveira, Sandra Helena Penha

Abstract

Objective: The objective of this study was to investigate the mediators and the resident peritoneal cells involved in the neutrophil migration (NM) induced by mineral trioxide aggregate (MTA) in mice. Study design: MTA (25 mg/cavity) was injected into normal and pretreated peritoneal cavities (PC) with indomethacin (IND), dexamethasone (DEX), BWA4C, U75302, antimacrophage inflammatory protein-2 (MIP-2), and anti-interleukin-1β (IL-1β) antibodies and the NM was determined. The role of macrophage (MO) and mast cells (MAST) was determined by administration of thioglycollate 3% or 48/80 compound, respectively. The concentration of IL-1β and MIP-2 exudates was measured by ELISA. Results: MTA induced dose- and time-dependent NM into mice PC, with the participation of MO and MAST. NM was inhibited by DEX, BWA4C, and U75302, as well as anti-MIP-2 and anti-IL-1β antibodies. In the exudates, IL-1β and MIP-2 were detected. Conclusions: This study suggests that MTA induces NM via a mechanism dependent on MAST and MO mediated by IL-1β, MIP-2, and LTB4. [Copyright &y& Elsevier]

Published
2008
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26. Mechanism of calcium hydroxide-induced neutrophil migration into air-pouch cavity.

Author

Costa MM, de Oliveira SH, and Gomes-Filho JE

Subjects
Air, Animals, Cytokines metabolism, Disease Models, Animal, Inflammation chemically induced, Male, Mice, Mice, Inbred BALB C, Prostaglandins metabolism, Subcutaneous Tissue, Calcium Hydroxide adverse effects, Chemotaxis, Leukocyte physiology, Inflammation immunology, Neutrophil Infiltration physiology, Root Canal Filling Materials adverse effects
Abstract

The aim of this study was to investigate cellular migration induced by calcium hydroxide to air-pouch cavities in mice. The migration was more specific to neutrophil and was dose and time dependent (peaking 96 h after stimulation). This migration was inhibited by pretreatment with thalidomide, indomethacin, MK886, meloxicam, dexamethasone, MK886 associated with indomethacin, and MK886 associated with indomethacin and dexamethasone. The air-pouch exudate from animals stimulated with calcium hydroxide showed an increase of leukotriene-B(4) (LTB(4)), interleukin-1beta, tumor necrosis factor alpha (TNF-alpha), cytokine-induced neutrophil chemoattractant (KC), and macrophage inflammatory protein 2 (MIP-2) release. Pretreatment with 3% thioglycollate increased the macrophage population in the air pouch but did not change neutrophil migration. Depleting the resident mast cells through chronic pretreatment with compound 48/80 did not alter neutrophil migration in response to calcium hydroxide. It was possible to conclude that calcium hydroxide-induced neutrophil migration to the air-pouch cavity in mice is mediated by LTB(4), TNF-alpha, KC, MIP-2, and prostaglandins, but it was not dependent on macrophages or mast cells.

Published
2008
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