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6. Identification and analysis of a novel microsatellite marker within the growth hormone gene promoter of Colossoma macropomum (Characiformes: Characidae) detected by TAIL-PCR

Author

Jaser, Suhaila K. K., Perazza, Caio A., Favaro, Leia C. L., Goto, Mariana A., de Oliveira, Alzira M., Hallerman, Eric M., Hilsdorf, Alexandre W. S., Jaser, Suhaila K. K., Perazza, Caio A., Favaro, Leia C. L., Goto, Mariana A., de Oliveira, Alzira M., Hallerman, Eric M., and Hilsdorf, Alexandre W. S.

Abstract

While in all vertebrates, growth hormone (GH) promotes post-natal growth, in fishes it also affects such metabolic functions as foraging rate, digestion, osmoregulation, and reproduction. The promoter region of the GH gene is an important target for studies of mechanisms regulating its expression, and polymorphisms within the promoter have been associated with performance traits in fishes. We used Thermal Asymmetric Interlaced PCR (TAIL-PCR) to amplify and sequence the 5′-flanking regions of the Colossoma macropomum GH (cmGH) gene. Based on a sequence of 1,038 bp, we designed three specific nested primers (R-290, R-186 and R-26) which were used with shorter arbitrary degenerate primers to amplify the 5′ proximal region of the cmGH gene. We identified a tetranucleotide (ATCC)4 microsatellite motif in this region, exhibiting four alleles (118, 122, 126 and 130 bp) within the population study. Genotypes at this locus deviated significantly from Hardy–Weinberg expectations (p ≤.05) and showed a low level of polymorphism (polymorphic information content = 0.163). High homozygosity (FIS = 0.147) was observed in the overall population. The polymorphism at the microsatellite makes it an important candidate for association studies between the respective genotypes, growth rate and other traits in farmed populations. Such studies may contribute to future breeding programs using marker-assisted selection upon this aquaculturally important species.

Published
2021
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7. O AGRONEGÓCIO BRASILEIRO NO COMÉRCIO INTERNACIONAL: VULNERABILIDADE, RETROCESSO, OPORTUNIDADE PERDIDA OU SITUAÇÃO ÓTIMA? UMA ANÁLISE DOS TRIÊNIOS (2007- 2009 e 2017-2019).

Author

Daiana Christ, Gabriela, de Oliveira, Alzira, Bresolin Galafassi, Leonardo, and Arruda Coronel, Daniel

Abstract

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Published
2022
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10. UVA/UVB-induced genotoxicity and lesion repair in Colossoma macropomum and Arapaima gigas Amazonian fish.

Author

Groff AA, da Silva J, Nunes EA, Ianistcki M, Guecheva TN, de Oliveira AM, de Oliveira CP, Val AL, and Henriques JA

Subjects
Animals, Comet Assay, Erythrocytes radiation effects, Mutagenicity Tests, DNA Damage, DNA Repair, Fishes genetics, Ultraviolet Rays
Abstract

Ultraviolet radiation is known to cause adverse effects to aquatic species and aquatic environments. The fish Colossoma macropomum (tambaqui) and Arapaima gigas (pirarucu) live in the Amazon basin, near the Equator, and thus receive high intensity of ultraviolet radiation. Deforestation further aggravates the situation by reducing shade at ground level. The aim of this study was to evaluate the genotoxic effects of UVA and UVB radiation on erythrocytes of tambaqui and pirarucu fish using Micronuclei test and Comet assay. Our study showed that UV radiation caused DNA damage in both species as detected by Comet assay. In addition, there were differences in response to genotoxicity between both species, which are possibly related to their evolutionary history. Tambaqui fish exposed to ultraviolet radiation for different periods presented clear dose-response in DNA damage profile. Significant damage repair was observed 24h after cessation of ultraviolet radiation exposure. At the test conditions used, no significant increase in micronucleated cells was observed in tambaqui and pirarucu fish. Tambaqui proved to be more sensitive to ultraviolet radiation than Pirarucu, as detected by Comet assay, showing statistically higher baseline DNA damage. The present results demonstrated that alkaline Comet assay was very sensitive for detecting the UV-induced genotoxicity during the short exposure period in our study. In addition, the present study also suggests that tambaqui and pirarucu fish are useful sentinel organisms, as their UV sensitivity allows them to be effective monitors of biological hazards in the Amazon region., (Copyright 2010 Elsevier B.V. All rights reserved.)

Published
2010
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