1. Evaluation of cleaning and sterilization of liposuction cannulas after intentional contamination with human fat, Mycobacterium abscessus subspecies bolletii, and Geobacillusstearothermophilus.
- Author
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Bronzatti JAG, de Souza RQ, Niero CV, Romagnoli CL, da Silva NM, de Moraes Bruna CQ, Gioielli LA, and Graziano KU
- Subjects
- Humans, Cannula, Steam, Sterilization methods, Mycobacterium abscessus, Lipectomy methods
- Abstract
Background: The complex design of cannulas is a major challenge for reprocessing due to the retention and accumulation of fat residues., Aim: To evaluate the cleaning of liposuction cannulas and the protective effects of the remaining fat residues on Mycobacterium abscessus subspecies bolletii (MASB) and Geobacillus stearothermophilus inactivation during steam sterilization., Methods: In phase 1, six standard operating procedures were evaluated for cleaning of liposuction cannulas. In phase 2, the sectioned lumens of the liposuction cannulas were contaminated with the largest and smallest volume of human fat found in phase 1, with the addition of MASB. In phase 3, the same volumes of human fat from phase 2 were used to contaminate paper strips containing G. stearothermophilus., Findings: In phase 1 the residual amount of fat ranged from 6 to 52 mg. In phases 2 and 3, the minimum and maximum amounts of fat (6 and 50 mg) protected micro-organisms during steam sterilization at 134 °C for 1.5 and 3 min., Conclusion: Effective cleaning and sterilization of liposuction cannulas intentionally contaminated with human fat, MASB and G. stearothermophilus could not be performed., (Copyright © 2023 The Healthcare Infection Society. Published by Elsevier Ltd. All rights reserved.)
- Published
- 2023
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