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Five ternary copper(II) complexes, [Cu 2 (phen) 2 (L1)(ClO 4 ) 2 ] (1), [Cu 2 (phen) 2 (L1)(DMSO) 2 ](PF6) 2 (2), [Cu 2 (bpy) 2 (L1)(ClO 4 ) 2 (H 2 O) 2 ] (3), [Cu 2 (dmp) 2 (L1)(ClO 4 ) 2 (H 2 O) 2 ] (4), and [Cu(phen)(L2)] 2 (ClO 4 ) 2 (5), in which phen = 1,10-phenanthroline, bpy = 2,2'-bipyridine, dmp = 2,9-dimethyl-1,10-phenanthroline, H 2 L1 = 1,4-dihydroxyanthracene-9,10-dione and HL2 = 1-hydroxyanthracene-9,10-dione, DMSO = dimethylsulfoxide, were synthesized and fully characterized. Complex 2 was obtained through the substitution of perchlorate for DMSO. When two hydroxyquinone groups are present, L1 makes a bridge between two Cu(II) ions, which also bind two nitrogens of the respective diimine ligand. The compounds bind to calf thymus DNA and oxidatively cleave pUC19 DNA according to the following order of activity 1 > 4-5 > 3. Furthermore, complexes 1, 3, 4 and 5 inhibit topoisomerase-I activity and the growth of myelogenous leukemia cells with the IC 50 values of 1.13, 10.60, 0.078, and 1.84 μmol L -1 , respectively. Complexes 1 and 4 are the most active in cancer cells and in DNA cleavage., Competing Interests: Declaration of Competing Interest The authors confirm that there is no conflict of interest in Antitumor Copper(II) Complexes with Hydroxyanthraquinones and N,NHeterocyclic Ligands authored by Ívina P. de Souza, Ariane C.C. de Melo, Bernardo L. Rodrigues, Adailton Bortoluzzi, Zara Molphy, Vickie McKee, Andrew Kellett, Rodrigo B. Fazzi, Ana M. da Costa Ferreira, and myself, which we submit for publication on the Journal of Inorganic Biochemistry., (Copyright © 2023 Elsevier Inc. All rights reserved.)

Background: Pseudomonas aeruginosa is an opportunistic pathogen and one of the leading causes of nosocomial infections. Moreover, the species can cause severe infections in cystic fibrosis patients, in burnt victims and cause disease in domestic animals. The control of these infections is often difficult due to its vast repertoire of mechanisms for antibiotic resistance. Phage therapy investigation with P. aeruginosa bacteriophages has aimed mainly the control of human diseases. In the present work, we have isolated and characterized a new bacteriophage, named Pseudomonas phage BrSP1, and investigated its host range against 36 P. aeruginosa strains isolated from diseased animals and against P. aeruginosa ATCC strain 27853., Results: We have isolated a Pseudomonas aeruginosa phage from sewage. We named this virus Pseudomonas phage BrSP1. Our electron microscopy analysis showed that phage BrSP1 had a long tail structure found in members of the order Caudovirales. "In vitro" biological assays demonstrated that phage BrSP1 was capable of maintaining the P. aeruginosa population at low levels for up to 12 h post-infection. However, bacterial growth resumed afterward and reached levels similar to non-treated samples at 24 h post-infection. Host range analysis showed that 51.4% of the bacterial strains investigated were susceptible to phage BrSP1 and efficiency of plating (EOP) investigation indicated that EOP values in the strains tested varied from 0.02 to 1.72. Analysis of the phage genome revealed that it was a double-stranded DNA virus with 66,189 bp, highly similar to the genomes of members of the genus Pbunavirus, a group of viruses also known as PB1-like viruses., Conclusion: The results of our "in vitro" bioassays and of our host range analysis suggested that Pseudomonas phage BrSP1 could be included in a phage cocktail to treat veterinary infections. Our EOP investigation confirmed that EOP values differ considerably among different bacterial strains. Comparisons of complete genome sequences indicated that phage BrSP1 is a novel species of the genus Pbunavirus. The complete genome of phage BrSP1 provides additional data that may help the broader understanding of pbunaviruses genome evolution.

Two new complexes of Ru(II) with mixed ligands were prepared: [Ru(bpy) 2 smp](PF 6 ) ( 1 ) and [Ru(phen) 2 smp](PF 6 ) ( 2 ), in which smp = sulfamethoxypyridazine; bpy = 2,2'-bipyridine; phen = 1,10-phenanthroline. The complexes have been characterized by elemental and conductivity analyses; infrared, NMR, and electrospray ionization mass spectroscopies; and X-ray diffraction of single crystal. Structural analyses reveal a distorted octahedral geometry around Ru(II) that is bound to two bpy (in 1 ) or two phen (in 2 ) via their two heterocyclic nitrogens and to two nitrogen atoms from sulfamethoxypyridazine-one of the methoxypyridazine ring and the sulfonamidic nitrogen, which is deprotonated. Both complexes inhibit the growth of chronic myelogenous leukemia cells. The interaction of the complexes with bovine serum albumin and DNA is described. DNA footprinting using an oligonucleotide as substrate showed the complexes' preference for thymine base rich sites. It is worth notifying that the complexes interact with the Src homology SH3 domain of the Abl tyrosine kinase protein. Abl protein is involved in signal transduction and implicated in the development of chronic myelogenous leukemia. Nuclear magnetic resonance (NMR) studies of the interaction of complex 2 with the Abl-SH3 domain showed that the most affected residues were T79, G97, W99, and Y115.

In this paper, we report the development of a novel technique in which the magnetoresistance of nanostructures is perturbatively measured by transversally modulating the DC magnetic field. It measures the electrical transport counterpart of the transverse magnetic AC-susceptibility. The technique was developed in a conventional four-probe configuration in which a small DC current flows through the sample and a small transverse AC-field perturbates the equilibrium position of the sample magnetization. Lock-in detection, in-phase with the AC-perturbation, is used to measure the voltage signal between the two inner electrodes of the four-probe station. We successfully demonstrated that this signal is proportional to the product of the first derivative of sample resistance with respect to the equilibrium position of the magnetization times the second derivative of the energy with respect to the equilibrium position of the magnetization. These dependences add new features to the technique investigated here that were not captured by the investigations previously reported on the same subject. To show the effective use of the technique, we discuss its application in measuring magnetic properties of thin magnetic films in the non-saturated regime.

Objective: Fresh sugarcane has been a new roughage source for ruminant's in semiarid regions, a function of the decline of sugar and alcohol industry in recent years. However, there is little data published regarding lambs fed sugarcane associated with spineless cactus. This study evaluated the effect of sugarcane replacement with spineless cactus (0%, 33%, 66%, and 100%) in the diet of Santa Inês lambs on carcass characteristics., Methods: Thirty-six non-castrated Santa Ines lambs at four months of age and an initial body weight of 22±2.3 kg were assigned in a randomized block design and slaughtered after 70 days of confinement. The effects of spineless cactus as a replacement for sugarcane in the diet of the lambs on the carcass characteristics, commercial cut weight and yield, leg tissue composition, and carcass measurements were studied., Results: The study revealed quadratic behavior in slaughter body weight, and hot and cold carcass weight, with maximum values of 38.60, 18.60, and 18.11 kg and replacement levels of 40.18%, 44.42%, and 43.14%, respectively. The cold carcass yield presented an increasing linear behavior. The compactness index of carcass and leg presented a quadratic effect, with estimated maximal values of 0.28 and 0.57 kg/cm and replacement levels of 43.37% and 45.5%, respectively. The weights of commercial cuts of leg, loin, shoulder, and breast showed quadratic behavior, with maximum values of 2.79, 0.852, 1.46, and 1.30 kg and replacement levels of 49.5, 45.32, 39.0, and 40.7, respectively. For tissue composition, quadratic behavior was verified for leg weight, subcutaneous fat, and total fat., Conclusion: The replacement of sugarcane by spineless cactus at level 44% is recommended for finishing lambs considering that this level improved most of the carcass characteristics, weights, and yields of commercial cuts and leg tissue composition.