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Two double-labelling methods, Tunel + propidium iodide and Annexin V-FITC + propidium iodide, have been tested to diagnose cell degradation processes in N1-N2 nauplius stages produced by spawning females of the copepod Calanus helgolandicus fed either non-toxic (PRO: control) or toxic (TR1) diets under laboratory conditions. Observation of labelled samples with a confocal laser-scanning microscope revealed that the maternal-food effect, following absorption of the toxic diet, induced cell apoptosis and necrosis in 80-100% of the offspring, which sooner or later died. Similar cell damages were also observed in 18-50% of the nauplii produced by females incubated in filtered seawater. Such cell degradation processes and high mortality were not observed in nauplii produced by females fed the non-toxic diet. Our protocols allowed detection of cell degradations before death of unhealthy larvae, preferentially with a confocal microscope or a conventional visible-fluorescent-light microscope.

The generic positions of the 29 Indo-West Pacific species currently placed in Rochinia A. Milne-Edwards, 1875, sensu lato, are addressed, in an attempt to establish a more phylogenetically coherent classification for these spider crabs. Twenty-five Indo-West Pacific species are referred to a redefined Samadinia Ng & Richer de Forges, 2013. Three species are transferred to Laubierinia Richer de Forges & Ng, 2009, Pugettia Dana, 1851, and Oxypleurodon Miers, 1885, respectively. Rochinia kagoshimensis (Rathbun, 1932) and a new species from the South China Sea are assigned to a new genus. The generic status of four Atlantic species of Rochinia is also discussed. [ABSTRACT FROM AUTHOR]

For the century to 2018, only one species of the deep-water porter crab Gordonopsis Guinot & Richer de Forges, 1995 (Brachyura, Homolidae), the type species, G. profundorum (Alcock & Anderson, 1899), was known, and only from a handful of specimens from the Indian Ocean. In 2019, two species were described from the eastern Indian and western Pacific Oceans. The present revision of available material, most of which was only collected in the last decade, adds five new species to the genus. This explosion in species numbers demonstrates just how poor our understanding is of deep-sea habitats and their constituent fauna. [ABSTRACT FROM AUTHOR]

The French MUSORSTOM expeditions (now Tropical Deep-Sea Benthos), have successfully collected since 1976 rich samples of large, benthic crustaceans using oceanographic vessels to depths of about 5,000 m. The ship-based collecting techniques and gear used by these expeditions are described. These include the use of echosounders, dredging, trawling, and trapping. Also discussed are techniques for onboard specimen sorting and photography. [ABSTRACT FROM AUTHOR]

The taxonomy of spider crabs of the genus Maja Lamarck, 1801, is revised, and a total of 36 species in 10 genera are now recognised from the eastern Atlantic, Mediterranean and Indo-West Pacific. The present revision describes seven genera and 17 species as new. Two genera previously synonymised under Maja: Paramaya De Haan, 1837, and Paramaja Kubo, 1936, are here treated as valid taxa. The confused nomenclature of Cancer cornutus Linnaeus, 1758, is resolved, and the name replaces Maja capensis Ortmann, 1894, and Mamaia queketti Stebbing, 1908. All genera and species are diagnosed and figured, and keys are provided for their identification. [ABSTRACT FROM AUTHOR]