10 results on '"da Silva, Luiz F. F."'
Search Results
2. Oral lesions and SARS‐CoV‐2: A postmortem study.
- Author
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Zarpellon, Amanda, Matuck, Bruno F., Dolhnikoff, Marisa, Duarte‐Neto, Amaro N., Maia, Gilvan, Gomes, Sara C., Sendyk, Daniel I., Souza, Suzana C. O. M., Mauad, Thais, Saldiva, Paulo H. N., Braz‐Silva, Paulo H., and da Silva, Luiz F. F.
- Subjects
RNA analysis ,ORAL microbiology ,REVERSE transcriptase polymerase chain reaction ,INTENSIVE care units ,PHYSICAL diagnosis ,COVID-19 ,BRONCHOALVEOLAR lavage ,STAINS & staining (Microscopy) ,CANKER sores ,SARS-CoV-2 ,ACADEMIC medical centers ,BIOPSY ,AUTOPSY ,IMMUNOHISTOCHEMISTRY ,MUCOUS membranes ,ARTIFICIAL respiration ,COMPARATIVE studies ,MIXED infections ,RESEARCH funding ,DESCRIPTIVE statistics ,AMYGDALOID body ,HERPESVIRUSES ,ORAL manifestations of general diseases ,ANGIOTENSIN converting enzyme - Abstract
The article presents a clinical-pathological report on autopsies of patients who died of COVID-19 in order to elucidate the possibility of oral manifestations of COVID-19. Topics include how the SARS-CoV-2 enters the oral cavity, essential studies to a correct link between oral manifestations and SARS-CoV-2 infection, and oral manifestations of COVID-19 found in postmortem case report.
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- 2022
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3. Oral lesions and SARS‐CoV‐2: A postmortem study
- Author
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Zarpellon, Amanda, primary, Matuck, Bruno F., additional, Dolhnikoff, Marisa, additional, Duarte‐Neto, Amaro N., additional, Maia, Gilvan, additional, Gomes, Sara C., additional, Sendyk, Daniel I., additional, Souza, Suzana C. O. M., additional, Mauad, Thais, additional, Saldiva, Paulo H. N., additional, Braz‐Silva, Paulo H., additional, and da Silva, Luiz F. F., additional
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- 2021
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4. Testicular pathology in fatal COVID‐19: A descriptive autopsy study
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Duarte‐Neto, Amaro N., primary, Teixeira, Thiago A., additional, Caldini, Elia G., additional, Kanamura, Cristina T., additional, Gomes‐Gouvêa, Michele S., additional, dos Santos, Angela B. G., additional, Monteiro, Renata A. A., additional, Pinho, João R. R., additional, Mauad, Thais, additional, da Silva, Luiz F. F., additional, Saldiva, Paulo H. N., additional, Dolhnikoff, Marisa, additional, Leite, Katia R. M., additional, and Hallak, Jorge, additional
- Published
- 2021
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5. Pulmonary and systemic involvement in COVID‐19 patients assessed with ultrasound‐guided minimally invasive autopsy
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Duarte‐Neto, Amaro N, primary, Monteiro, Renata A A, additional, da Silva, Luiz F F, additional, Malheiros, Denise M A C, additional, de Oliveira, Ellen P, additional, Theodoro‐Filho, Jair, additional, Pinho, João R R, additional, Gomes‐Gouvêa, Michele S, additional, Salles, Ana P M, additional, de Oliveira, Ilka R S, additional, Mauad, Thais, additional, Saldiva, Paulo H N, additional, and Dolhnikoff, Marisa, additional
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- 2020
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6. Lung Pathology in Fatal Novel Human Influenza A (H1N1) Infection
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Mauad, Thais, Hajjar, Ludhmila A., Callegari, Giovanna D., da Silva, Luiz F. F., Schout, Denise, Galas, Filomena R. B. G., Alves, Venancio A. F., Malheiros, Denise M. A. C., Auler, Jose O. C., Jr., Ferreira, Aurea F., Borsato, Marcela R. L., Bezerra, Stephania M., Gutierrez, Paulo S., Caldini, Elia T. E. G., Pasqualucci, Carlos A., Dolhnikoff, Marisa, and Saldiva, Paulo H. N.
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- 2010
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7. Testicular pathology in fatal COVID‐19: A descriptive autopsy study.
- Author
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Duarte‐Neto, Amaro N., Teixeira, Thiago A., Caldini, Elia G., Kanamura, Cristina T., Gomes‐Gouvêa, Michele S., dos Santos, Angela B. G., Monteiro, Renata A. A., Pinho, João R. R., Mauad, Thais, da Silva, Luiz F. F., Saldiva, Paulo H. N., Dolhnikoff, Marisa, Leite, Katia R. M., and Hallak, Jorge
- Subjects
SERTOLI cells ,LEYDIG cells ,AUTOPSY ,CELL adhesion molecules ,VIRAL antigens - Abstract
Background: Multi‐organ damage is a common feature of severe acute respiratory syndrome coronavirus 2 (SARS‐CoV‐2) infection, going beyond the initially observed severe pneumonia. Evidence that the testis is also compromised is growing. Objective: To describe the pathological findings in testes from fatal cases of COVID‐19, including the detection of viral particles and antigens, and inflammatory cell subsets. Materials and methods: Postmortem testicular samples were obtained by percutaneous puncture from 11 deceased men and examined by reverse‐transcription polymerase chain reaction (RT‐PCR) for RNA detection and by light and electron microscopy (EM) for SARS‐CoV‐2. Immunohistochemistry (IHC) for the SARS‐CoV‐2 N‐protein and lymphocytic and histiocytic markers was also performed. Results: Eight patients had mild interstitial orchitis, composed mainly of CD68+ and TCD8+ cells. Fibrin thrombi were detected in five cases. All cases presented congestion, interstitial edema, thickening of the tubular basal membrane, decreased Leydig and Sertoli cells with reduced spermatogenesis, and strong expression of vascular cell adhesion molecule (VCAM) in vessels. IHC detected SARS‐Cov‐2 antigen in Leydig cells, Sertoli cells, spermatogonia, and fibroblasts in all cases. EM detected viral particles in the cytoplasm of fibroblasts, endothelium, Sertoli and Leydig cells, spermatids, and epithelial cells of the rete testis in four cases, while RT‐PCR detected SARS‐CoV‐2 RNA in three cases. Discussion and conclusion: The COVID‐19‐associated testicular lesion revealed a combination of orchitis, vascular changes, basal membrane thickening, Leydig and Sertoli cell scarcity, and reduced spermatogenesis associated with SARS‐CoV‐2 local infection that may impair hormonal function and fertility in men. [ABSTRACT FROM AUTHOR]
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- 2022
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8. Comparison of different injection volumes on spread of lumbar erector spinae plane block: An anatomical study.
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Azevedo AS, Silva VTG, Xavier AL, da Silva LFF, Hojaij FC, Ashmawi HA, Vieira JE, and Fernandes HS
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- Humans, Lumbosacral Region, Pain, Postoperative etiology, Pain, Postoperative prevention & control, Nerve Block adverse effects, Paraspinal Muscles diagnostic imaging
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- 2021
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9. Small airway remodeling in acute respiratory distress syndrome: a study in autopsy lung tissue.
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Morales MM, Pires-Neto RC, Inforsato N, Lanças T, da Silva LF, Saldiva PH, Mauad T, Carvalho CR, Amato MB, and Dolhnikoff M
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- Adult, Autopsy, Case-Control Studies, Extracellular Matrix metabolism, Female, Humans, Male, Middle Aged, Retrospective Studies, Airway Remodeling physiology, Lung pathology, Respiratory Distress Syndrome physiopathology
- Abstract
Introduction: Airway dysfunction in patients with the Acute Respiratory Distress Syndrome (ARDS) is evidenced by expiratory flow limitation and dynamic hyperinflation. These functional alterations have been attributed to closure/obstruction of small airways. Airway morphological changes have been reported in experimental models of acute lung injury, characterized by epithelial necrosis and denudation in distal airways. To date, however, no study has focused on the morphological airway changes in lungs from human subjects with ARDS. The aim of this study is to evaluate structural and inflammatory changes in distal airways in ARDS patients., Methods: We retrospectively studied autopsy lung tissue from subjects who died with ARDS and from control subjects who died of non pulmonary causes. Using image analysis, we quantified the extension of epithelial changes (normal, abnormal and denudated epithelium expressed as percentages of the total epithelium length), bronchiolar inflammation, airway wall thickness, and extracellular matrix (ECM) protein content in distal airways. The Student's t-test or the Mann-Whitney test was used to compare data between the ARDS and control groups. Bonferroni adjustments were used for multiple tests. The association between morphological and clinical data was analyzed by Pearson rank test., Results: Thirty-one ARDS patients (A: PaO2/FiO2 ≤200, 45 ± 14 years, 16 males) and 11 controls (C: 52 ± 16 years, 7 males) were included in the study. ARDS airways showed a shorter extension of normal epithelium (A:32.9 ± 27.2%, C:76.7 ± 32.7%, P < 0.001), a larger extension of epithelium denudation (A:52.6 ± 35.2%, C:21.8 ± 32.1%, P < 0.01), increased airway inflammation (A:1(3), C:0(1), P = 0.03), higher airway wall thickness (A:138.7 ± 54.3 μm, C:86.4 ± 33.3 μm, P < 0.01), and higher airway content of collagen I, fibronectin, versican and matrix metalloproteinase-9 (MMP-9) compared to controls (P ≤0.03). The extension of normal epithelium showed a positive correlation with PaO2/FiO2 (r2 = 0.34; P = 0.02) and a negative correlation with plateau pressure (r2 = 0.27; P = 0.04). The extension of denuded epithelium showed a negative correlation with PaO2/FiO2 (r2 = 0.27; P = 0.04)., Conclusions: Structural changes in small airways of patients with ARDS were characterized by epithelial denudation, inflammation and airway wall thickening with ECM remodeling. These changes are likely to contribute to functional airway changes in patients with ARDS.
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- 2011
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10. The outer wall of small airways is a major site of remodeling in fatal asthma.
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Dolhnikoff M, da Silva LF, de Araujo BB, Gomes HA, Fernezlian S, Mulder A, Lindeman JH, and Mauad T
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- Adult, Asthma immunology, Asthma metabolism, Collagen Type I analysis, Collagen Type I immunology, Collagen Type III analysis, Collagen Type III immunology, Extracellular Matrix immunology, Extracellular Matrix metabolism, Female, Fibronectins analysis, Fibronectins immunology, Humans, Lung immunology, Lung metabolism, Male, Matrix Metalloproteinases analysis, Matrix Metalloproteinases immunology, Middle Aged, Tissue Inhibitor of Metalloproteinases analysis, Tissue Inhibitor of Metalloproteinases immunology, Asthma pathology, Extracellular Matrix pathology, Lung pathology
- Abstract
Background: Structural and inflammatory changes in asthma involve both the large and small airways, with involvement of the distal lung being related to disease severity. We have previously shown that changes in the extracellular matrix (ECM) composition of the distal lung are associated with loss of alveolar attachments in patients with fatal asthma. However, major ECM elements, such as collagen I and fibronectin and their regulators, have not been addressed at the distal level., Objective: We sought to evaluate ECM remodeling in the distal lungs of asthmatic patients., Methods: Using immunohistochemistry and image analysis, we determined the content of collagen I and III, fibronectin, and matrix metalloproteinases (MMPs) 1, 2, and 9 and tissue inhibitors of metalloproteinase (TIMPs) 1 and 2 in the large and small airways and lung parenchyma of 24 patients with fatal asthma and compared the results with those of 11 nonasthmatic control subjects. Protein content was defined as the area of positive staining divided by basement membrane or septum length., Results: We observed increased collagen I and decreased collagen III content in the small airways of asthmatic patients compared with that seen in control subjects. Greater fibronectin and MMP-1, MMP-2, and MMP-9 content was observed at the outer area of the small airways in asthmatic patients. MMP content was also increased in the peribronchiolar parenchyma in asthmatic patients. In contrast, TIMP expression was only increased in the large airways of asthmatic patients compared with that seen in control subjects., Conclusions: The outer area of the small airways is a major site of ECM remodeling in fatal asthma, potentially contributing to functional changes and the loss of airway-parenchyma interdependence observed in patients with fatal asthma.
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- 2009
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