9 results on '"da Silva, Katia Regina"'
Search Results
2. Application of a novel Paenibacillus-specific PCR-DGGE method and sequence analysis to assess the diversity of Paenibacillus spp. in the maize rhizosphere
- Author
-
Araújo da Silva, Katia Regina, Salles, Joana Falcão, Seldin, Lucy, and van Elsas, Jan Dirk
- Published
- 2003
- Full Text
- View/download PDF
Catalog
3. Quality of life in patients with implantable cardioverter–defibrillator: systematic review of randomized controlled trials
- Author
-
da Silva, Katia Regina, primary, Costa, Roberto, additional, Rodrigues, Clarissa Garcia, additional, Schasechter, Andi, additional, Nobre, Moacyr Cuce, additional, Passman, Rod, additional, and Mark, Daniel B, additional more...
- Published
- 2017
- Full Text
- View/download PDF
4. Quality of life in patients with implantable cardioverter–defibrillator: systematic review of randomized controlled trials.
- Author
-
da Silva, Katia Regina, Costa, Roberto, Rodrigues, Clarissa Garcia, Schasechter, Andi, Nobre, Moacyr Cuce, Passman, Rod, and Mark, Daniel B.
- Subjects
- *
HEART failure treatment , *PSYCHOLOGY of cardiac patients , *IMPLANTABLE cardioverter-defibrillators , *INFORMATION storage & retrieval systems , *MEDICAL databases , *MEDICAL information storage & retrieval systems , *MEDLINE , *ONLINE information services , *QUALITY of life , *SYSTEMATIC reviews ,CARDIAC arrest prevention - Abstract
Background: Implantable cardioverter–defibrillator (ICD) therapy significantly improves the survival of patients who are at high risk for sudden cardiac death. However, it is unclear whether this survival is accompanied by impairment on quality of life (QoL). Objectives: This systematic review sought to describe whether ICD therapy, as compared with standard treatment, can have an impact on QoL outcomes. Methods: Extensive literature searches were carried out in PubMed, EMBASE, LILACS and Cochrane Library. Eligible studies were randomized controlled trials (RCTs) of ICD versus medical therapy that reported valid and reliable measures of QoL. Included studies were reviewed to determine baseline patient characteristics, mean duration of follow-up, questionnaires used to assess QoL and association between QoL scores and ICD shock therapy. Results: Seven studies, enrolling a total of 5,701 patients, were included in this review. The analyzed trials showed conflicting results about the impact of ICD on QoL outcomes. Among the secondary prevention studies, CIDS reported a clear benefit from ICD and AVID showed no difference between ICD and amiodarone groups. Of the primary prevention trials, AMIOVIRT, MADIT II, DEFINITE, and SCD-HeFT found no evidence of impaired QoL in patients with an ICD. Evidence for an association between ICD shocks and QoL was mixed and seemed to depend on the interval between shocks and QoL assessment. Conclusion: There was no evidence of impaired QoL in patients with an ICD. However, ICD patients must be educated of all possible risks and benefits, including transitory declines in QoL after ICD shocks. [ABSTRACT FROM AUTHOR] more...
- Published
- 2018
- Full Text
- View/download PDF
5. Timing to Intubation COVID-19 Patients: Can We Put It Off until Tomorrow?
- Author
-
de Alencar, Júlio César Garcia, Sternlicht, Juliana Martes, Veiga, Alicia Dudy Muller, Marchini, Julio Flávio Meirelles, Ferreira, Juliana Carvalho, de Carvalho, Carlos Roberto Ribeiro, Marcilio, Izabel, da Silva, Katia Regina, Cobello Junior, Vilson, Felix, Marcelo Consorti, Gomez, Luz Marina Gomez, de Souza, Heraldo Possolo, Mauá, Denis Deratani, Emergency USP COVID Group, and HCFMUSP COVID-19 Study Group more...
- Subjects
COVID-19 ,ARTIFICIAL respiration ,INTUBATION ,MORTALITY ,MECONIUM aspiration syndrome ,DEATH rate - Abstract
Background: The decision to intubate COVID-19 patients receiving non-invasive respiratory support is challenging, requiring a fine balance between early intubation and risks of invasive mechanical ventilation versus the adverse effects of delaying intubation. This present study analyzes the association between intubation day and mortality in COVID-19 patients. Methods: We performed a unicentric retrospective cohort study considering all COVID-19 patients consecutively admitted between March 2020 and August 2020 requiring invasive mechanical ventilation. The primary outcome was all-cause mortality within 28 days after intubation, and a Cox model was used to evaluate the effect of time from onset of symptoms to intubation in mortality. Results: A total of 592 (20%) patients of 3020 admitted with COVID-19 were intubated during study period, and 310 patients who were intubated deceased 28 days after intubation. Each additional day between the onset of symptoms and intubation was significantly associated with higher in-hospital death (adjusted hazard ratio, 1.018; 95% CI, 1.005–1.03). Conclusion: Among patients infected with SARS-CoV-2 who were intubated and mechanically ventilated, delaying intubation in the course of symptoms may be associated with higher mortality. Trial registration: The study protocol was approved by the local Ethics Committee (opinion number 3.990.817; CAAE: 30417520.0.0000.0068). [ABSTRACT FROM AUTHOR] more...
- Published
- 2022
- Full Text
- View/download PDF
6. Distinct Outcomes in COVID-19 Patients with Positive or Negative RT-PCR Test.
- Author
-
Saad Menezes, Maria Clara, Santinelli Pestana, Diego Vinicius, Ferreira, Juliana Carvalho, Ribeiro de Carvalho, Carlos Roberto, Felix, Marcelo Consorti, Marcilio, Izabel Oliva, da Silva, Katia Regina, Junior, Vilson Cobello, Marchini, Julio Flavio, Alencar, Julio Cesar, Gomez, Luz Marina Gomez, Mauá, Denis Deratani, and Souza, Heraldo Possolo more...
- Subjects
COVID-19 ,REVERSE transcriptase polymerase chain reaction ,TREATMENT effectiveness ,SARS-CoV-2 ,INTENSIVE care units - Abstract
Identification of the SARS-CoV-2 virus by RT-PCR from a nasopharyngeal swab sample is a common test for diagnosing COVID-19. However, some patients present clinical, laboratorial, and radiological evidence of COVID-19 infection with negative RT-PCR result(s). Thus, we assessed whether positive results were associated with intubation and mortality. This study was conducted in a Brazilian tertiary hospital from March to August of 2020. All patients had clinical, laboratory, and radiological diagnosis of COVID-19. They were divided into two groups: positive (+) RT-PCR group, with 2292 participants, and negative (−) RT-PCR group, with 706 participants. Patients with negative RT-PCR testing and an alternative most probable diagnosis were excluded from the study. The RT-PCR(+) group presented increased risk of intensive care unit (ICU) admission, mechanical ventilation, length of hospital stay, and 28-day mortality, when compared to the RT-PCR(−) group. A positive SARS-CoV-2 RT-PCR result was independently associated with intubation and 28 day in-hospital mortality. Accordingly, we concluded that patients with a COVID-19 diagnosis based on clinical data, despite a negative RT-PCR test from nasopharyngeal samples, presented more favorable outcomes than patients with positive RT-PCR test(s). [ABSTRACT FROM AUTHOR] more...
- Published
- 2022
- Full Text
- View/download PDF
7. Temporary atrial pacing in the prevention of postoperative atrial fibrillation (vol 30, pg S79, 2007)
- Author
-
Neto, Vicente Avila, Roberto Costa, Da Silva, Katia Regina, Martins, Andre Luiz Mendes, Escobar, Luiz Fernando, Moreira, Luiz Felipe Pinho, Costa, Regina Valeria, Santos, Leticia Bezerra, and Melo, Ricardo Fernandes Azevedo more...
8. Post-acute sequelae of SARS-CoV-2 infection (PASC): a protocol for a multidisciplinary prospective observational evaluation of a cohort of patients surviving hospitalisation in Sao Paulo, Brazil.
- Author
-
Busatto GF, de Araújo AL, Duarte AJDS, Levin AS, Guedes BF, Kallas EG, Pinna FR, de Souza HP, da Silva KR, Sawamura MVY, Seelaender M, Imamura M, Garcia ML, Forlenza OV, Nitrini R, Damiano RF, Rocha VG, Batisttella LR, and Carvalho CRR more...
- Subjects
- Brazil, Hospitalization, Humans, Observational Studies as Topic, Post-Acute COVID-19 Syndrome, COVID-19 complications, SARS-CoV-2
- Abstract
Introduction: COVID-19 may lead to persistent and potentially incapacitating clinical manifestations (post-acute sequelae of SARS-CoV-2 infection (PASC)). Using easy-to-apply questionnaires and scales (often by telephone interviewing), several studies evaluated samples of COVID-19 inpatients from 4 weeks to several months after discharge. However, studies conducting systematic multidisciplinary assessments of PASC manifestations are scarce, with thorough in-person objective evaluations restricted to modestly sized subsamples presenting greatest disease severity., Methods and Analyses: We will conduct a prospective observational study of surviving individuals (above 18 years of age) from a cohort of over 3000 subjects with laboratory-confirmed COVID-19 who were treated as inpatients at the largest academic health centre in Sao Paulo, Brazil (Hospital das Clínicas, Faculdade de Medicina, Universidade de São Paulo). All eligible subjects will be consecutively invited to undergo a 1-2-day series of multidisciplinary assessments at 2 time-points, respectively, at 6-9 months and 12-15 months after discharge. Assessment schedules will include detailed multidomain questionnaires applied by medical research staff, self-report scales, objective evaluations of cardiopulmonary functioning, physical functionality and olfactory status, standardised neurological, psychiatric and cognitive examinations, as well as diagnostic laboratory, muscle ultrasound and chest imaging exams. Remaining material from blood tests will be incorporated by a local biobank for use in future investigations on inflammatory markers, genomics, transcriptomics, peptidomics and metabolomics., Ethics and Dissemination: All components of this programme have been approved by local research ethics committees. We aim to provide insights into the frequency and severity of chronic/post-COVID multiorgan symptoms, as well as their interrelationships and associations with acute disease features, sociodemographic variables and environmental exposures. Findings will be disseminated in peer-reviewed journals and at scientific meetings. Additionally, we aim to provide a data repository to allow future pathophysiological investigations relating clinical PASC features to biomarker data extracted from blood samples., Trial Registration Number: RBR-8z7v5wc; Pre-results., Competing Interests: Competing interests: None declared., (© Author(s) (or their employer(s)) 2021. Re-use permitted under CC BY-NC. No commercial re-use. See rights and permissions. Published by BMJ.) more...
- Published
- 2021
- Full Text
- View/download PDF
9. Application of a novel Paenibacillus-specific PCR-DGGE method and sequence analysis to assess the diversity of Paenibacillus spp. in the maize rhizosphere.
- Author
-
da Silva KR, Salles JF, Seldin L, and van Elsas JD
- Subjects
- Base Sequence, DNA Fingerprinting methods, DNA, Bacterial chemistry, Electrophoresis, Polyacrylamide Gel, Gram-Positive Endospore-Forming Rods classification, Gram-Positive Endospore-Forming Rods genetics, Nitrogen Fixation, Nucleic Acid Denaturation, Phylogeny, Plant Roots microbiology, RNA, Ribosomal, 16S analysis, RNA, Ribosomal, 16S genetics, Reproducibility of Results, Research Design, Sequence Alignment, DNA, Bacterial analysis, Gram-Positive Endospore-Forming Rods isolation & purification, Polymerase Chain Reaction, Zea mays microbiology
- Abstract
In this study, a Paenibacillus-specific PCR system, based on the specific primer PAEN515F in combination with bacterial primer R1401, was tested and used to amplify specific fragments of the 16S rRNA gene from rhizosphere DNA. The amplicons were used in a second (semi-nested) PCR for DGGE, in which bacterial primers F968GC and R1401 were used. The resulting products were separated into community fingerprints by DGGE. To assess the reliability of the method, the diversity of Paenibacillus species was evaluated on the basis of DNA extracted directly from the rhizospheres of four different cultivars of maize (Zea mays), i.e. CMS04, CMS11, CMS22 and CMS36, sown in two Brazilian field soils (Cerrado and Várzea). In addition, a clone library was generated from the PCR-generated 16S rDNA fragments, and selected clones were sequenced. The results of the bacterial community analyses showed, at the level of clone libraries, that considerable diversity among Paenibacillus spp. was present. The most dominantly found sequences clustered into 12 groups, each one potentially representing a species complex. Sequences closely affiliated with the P. macerans and P. azotofixans complexes were found in all samples, whereas other sequences were scarcer. Clones affiliated with the latter species complex were most abundant, representing 19% of all clones analysed. The Paenibacillus fingerprints generated via semi-nested PCR followed by DGGE showed a clear distinction between the maize plants grown in Cerrado versus Várzea soils. Thus, soil type, instead of maize cultivar type, was the overriding determinative factor that influenced the community structures of the Paenibacillus communities in the rhizospheres investigated. At a lower level (subcluster), there was a trend for maize cultivars CMS11 and CMS22 on the one hand, and CMS36 and CMS04 on the other hand, to cluster together, indicating that these respective pair of cultivars were similar in their Paenibacillus species composition. This trend was tentatively linked to the growth characteristics of these maize cultivars. These results clearly demonstrated the efficacy of the Paenibacillus-specific PCR-DGGE method in describing Paenibacillus species diversity in rhizosphere soils. more...
- Published
- 2003
- Full Text
- View/download PDF
Discovery Service for Jio Institute Digital Library
For full access to our library's resources, please sign in.