6 results on '"da Silva, Gisele L"'
Search Results
2. Contribution of vanilloid receptors to the overt nociception induced by B2 kinin receptor activation in mice
- Author
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Ferreira, Juliano, da Silva, Gisele L, and Calixto, João B
- Subjects
Male ,Mice ,Dose-Response Relationship, Drug ,Receptor, Bradykinin B2 ,Receptors, Drug ,Papers ,Bradykinin B2 Receptor Antagonists ,Animals ,lipids (amino acids, peptides, and proteins) ,Capsaicin ,Bradykinin ,Pain Measurement - Abstract
1. The vanilloid receptor (TRPV1) is viewed as a molecular integrator of several nociceptive stimuli. In the present study, we have investigated the role played by TRPV1 in the nociceptive response induced by the peripheral activation of kinin B(2) receptor in mice. 2. The intraplantar (i.pl.) administration of bradykinin (BK) and the selective B(2) agonist Tyr(8)-BK, or the vanilloid agonists resiniferatoxin and capsaicin, into the mouse paw induced a dose-related overt nociception of short duration. The B(2) receptor antagonist Hoe 140 inhibited BK-induced, but not capsaicin-induced, nociceptive response. On the other hand, the TRPV1 antagonist capsazepine inhibited both capsaicin- and BK-mediated nociception. 3. Repeated injections of BK or capsaicin produced desensitization to their nociceptive response. Capsaicin desensitization greatly reduced BK-induced nociception, but in contrast, the desensitization to BK increased the capsaicin response. 4. Administration of low doses of capsaicin or acidified saline did not produce nociception when administered alone, but caused a pronounced effect when administered in association with a subthreshold dose of BK. Moreover, the degeneration of the subset of primary afferent fibers, sensitive to capsaicin, abolished both capsaicin- and BK-induced nociception. 5. The inhibition of phospholipase C (PLC), protein kinase C or phospholipase A(2) markedly decreased the nociception caused by BK, but not that of capsaicin. BK administration increased leukotriene B(4) levels in the injected paw. Likewise, BK-induced overt nociception was decreased by lipoxygenase (LOX) inhibition. 6. These results demonstrate that BK produces overt nociception mediated by TRPV1 receptor stimulation, via PLC pathway activation and LOX product formation.
- Published
- 2004
3. Contribution of vanilloid receptors to the overt nociception induced by B2kinin receptor activation in mice
- Author
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Ferreira, Juliano, primary, Da Silva, Gisele L, additional, and Calixto, João B, additional
- Published
- 2004
- Full Text
- View/download PDF
4. Contribution of vanilloid receptors to the overt nociception induced by B2 kinin receptor activation in mice.
- Author
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Ferreira, Juliano, da Silva, Gisele L., and Calixto, João B.
- Subjects
- *
NOCICEPTORS , *BRADYKININ , *CAPSAICIN , *PHOSPHOLIPASE C , *PROTEIN kinase C , *LEUKOTRIENES , *LIPOXYGENASES - Abstract
1 The vanilloid receptor (TRHY1) is viewed as a molecular integrator of several nociceptive stimuli. In the present study, we have investigated the role played by TRPV1 in the nociceptive response induced by the peripheral activation of kinin B2 receptor in mice. 2 The intraplantar (i.pl.) administration of bradykinin (BK) and the selective B2 agonist Tyr8-BK. or the vanilloid agonists resiniferatoxin and capsaicin, into the mouse paw induced a dose-related overt nociception of short duration. The B2 receptor antagonist Hoe 140 inhibited BK-induced, but not capsaicin-induced, nociceptive response. On the other hand, the TRPV1 antagonist capsazepine inhibited both capsaicin- and Bk-mediated nociception. 3 Repeated injections of HK or capsaicin produced desensitization to then- nociceptive response. Capsaicin desensitization greatly reduced BK-induced nociception, but in contrast, the desensitization to BK increased the capsaicin response. 4 Administration of low doses of capsaicin or acidified saline did not produce nociception when administered alone, but caused a pronounced effect when administered in association with a subthreshold dose of BK. Moreover, the degeneration of the subset of primary afferent fibers, sensitive to capsaicin. abolished both capsaicin- and BK-induced nociception. 5 The inhibition of phospholipase C (PLC), protein kinase C or phospholipase A- markedly decreased the nociception caused by BK, but not that of' capsaicin. BK administration increased leukotriene B4 levels in the injected paw. Likewise, BK-induced overt nociception was decreased by lipoxygenase (LOX) inhibition. 6 These results demonstrate that BK produces overt nociception mediated by TRPVI receptor stimulation, via PLC pathway activation and LOX product formation. [ABSTRACT FROM AUTHOR]
- Published
- 2004
- Full Text
- View/download PDF
5. <atl>Involvement of monoaminergic system in the antidepressant-like effect of the hydroalcoholic extract of Siphocampylus verticillatus
- Author
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Rodrigues, Ana Lúcia S., da Silva, Gisele L., Mateussi, Andreza S., Fernandes, Elizabeth S., Miguel, Obdulio G., Yunes, Rosendo A., Calixto, João B., and Santos, Adair R.S.
- Subjects
- *
MEDICINAL plants , *SEROTONIN - Abstract
The antidepressant-like effect of the hydroalcoholic extract obtained from aerial parts of Siphocampylus verticillatus, a Brazilian medicinal plant, was investigated in two models of depression in mice and against synaptosomal uptake of serotonin, noradrenaline and dopamine. The immobility times in the forced swimming test (FST) and in the tail suspension test (TST) were significantly reduced by the extract (dose range 100–1000 mg/kg, i.p.), without accompanying changes in ambulation when assessed in an open-field. In addition when given orally the extract was also effective in reducing the immobility time in the TST. The efficacy of extract in the TST was comparable to that of the tricyclic antidepressant imipramine (15 mg/kg, i.p.) and with fluoxetine (32 mg/kg, i.p.). The anti-immobility effect of the extract (600 mg/kg, i.p.) assessed in the TST was not affected by pre-treatment with naloxone (1 mg/kg, i.p., a non-selective opioid receptor antagonist) or L-arginine (750 mg/kg, i.p., a nitric oxide precursor). In contrast, the extract (600 mg/kg, i.p.) antidepressant-like effect was significantly reduced by pre-treatment of animals with p-chlorophenylalanine (PCPA, 100 mg/kg, i.p., an inhibitor of serotonin synthesis), sulpiride (50 mg/kg, i.p., a selective D2 receptor antagonist), prazosin (62.5 μg/kg, i.p., an α1 adrenoreceptor antagonist) or by guanosine 5′-monophosphate (GMP, 250 mg/kg, i.p., a nucleotide known to block some actions elicited by NMDA). The biochemical data show that the extract of S. verticillatus inhibited in a graded manner the uptake of monoamines. However, at the IC50 level, the extract was approximately 3.2 to 3.4-fold more potent and also more efficacious in inhibiting the synaptosomal uptake of noradrenaline and serotonin than dopamine. Taken together these data demonstrate that the extract of S. verticillatus elicited a significant antidepressant-like effect, when assessed in the TST and FST in mice. Its action seems to involve an interaction with adrenergic, dopaminergic, glutamatergic and serotonergic systems. [Copyright &y& Elsevier]
- Published
- 2002
- Full Text
- View/download PDF
6. Contribution of vanilloid receptors to the overt nociception induced by B2 kinin receptor activation in mice.
- Author
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Ferreira J, da Silva GL, and Calixto JB
- Subjects
- Animals, Bradykinin pharmacology, Bradykinin B2 Receptor Antagonists, Capsaicin pharmacology, Dose-Response Relationship, Drug, Male, Mice, Pain Measurement drug effects, Receptor, Bradykinin B2 agonists, Receptors, Drug agonists, Receptors, Drug antagonists & inhibitors, Pain Measurement methods, Receptor, Bradykinin B2 metabolism, Receptors, Drug metabolism
- Abstract
1. The vanilloid receptor (TRPV1) is viewed as a molecular integrator of several nociceptive stimuli. In the present study, we have investigated the role played by TRPV1 in the nociceptive response induced by the peripheral activation of kinin B(2) receptor in mice. 2. The intraplantar (i.pl.) administration of bradykinin (BK) and the selective B(2) agonist Tyr(8)-BK, or the vanilloid agonists resiniferatoxin and capsaicin, into the mouse paw induced a dose-related overt nociception of short duration. The B(2) receptor antagonist Hoe 140 inhibited BK-induced, but not capsaicin-induced, nociceptive response. On the other hand, the TRPV1 antagonist capsazepine inhibited both capsaicin- and BK-mediated nociception. 3. Repeated injections of BK or capsaicin produced desensitization to their nociceptive response. Capsaicin desensitization greatly reduced BK-induced nociception, but in contrast, the desensitization to BK increased the capsaicin response. 4. Administration of low doses of capsaicin or acidified saline did not produce nociception when administered alone, but caused a pronounced effect when administered in association with a subthreshold dose of BK. Moreover, the degeneration of the subset of primary afferent fibers, sensitive to capsaicin, abolished both capsaicin- and BK-induced nociception. 5. The inhibition of phospholipase C (PLC), protein kinase C or phospholipase A(2) markedly decreased the nociception caused by BK, but not that of capsaicin. BK administration increased leukotriene B(4) levels in the injected paw. Likewise, BK-induced overt nociception was decreased by lipoxygenase (LOX) inhibition. 6. These results demonstrate that BK produces overt nociception mediated by TRPV1 receptor stimulation, via PLC pathway activation and LOX product formation.
- Published
- 2004
- Full Text
- View/download PDF
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