1. Dried mulberry fruit ameliorates cardiovascular and liver histopathological changes in high-fat diet-induced hyperlipidemic mice
- Author
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Kittiwoot To-on, Piya Prajumwong, Nitra Nuengchamnong, Supaporn Puntheeranurak, Rachanee Chanasong, Watcharakorn Deetud, Suriya Chaiwong, Nanteetip Limpeanchob, Usana Chatturong, Ekarin Chulikorn, Julintorn Somran, Veerada Raksanoh, Kanittaporn Trisat, Kroekkiat Chinda, Krongkarn Chootip, and Tanwarat Kajsongkram
- Subjects
medicine.medical_specialty ,HDL, high-density lipoprotein ,0211 other engineering and technologies ,02 engineering and technology ,Bile acid binding ,Cardiovascular ,01 natural sciences ,Muscle hypertrophy ,Mulberry fruit ,chemistry.chemical_compound ,Internal medicine ,Adipocyte ,LDL, low-density lipoprotein ,021105 building & construction ,Hyperlipidemia ,Medicine ,DPPH, 2,2-diphenyl-1-picrylhydrazyl ,TG, triglyceride ,business.industry ,Autophagy ,Fatty liver ,High fat diet ,medicine.disease ,Morus alba ,0104 chemical sciences ,TC, total cholesterol ,010404 medicinal & biomolecular chemistry ,Endocrinology ,Complementary and alternative medicine ,chemistry ,Liver ,Original Article ,business ,dMF, Dried mulberry fruit ,Lipoprotein - Abstract
Background and aim Metabolic disease encompasses most contemporary non-communicable diseases, especially cardiovascular and fatty liver disease. Mulberry fruits of Morus alba L. are a favoured food and a traditional medicine. While they are anti-atherosclerotic and reduce hyperlipidemic risk factors, studies need wider scope that include ameliorating cardiovascular and liver pathologies if they are to become clinically effective treatments. Therefore, the present study sought to show that freshly dried mulberry fruits (dMF) might counteract the metabolic/cardiovascular pathologies in mice made hyperlipidemic by high-fat diet (HF). Experimental procedure C57BL/6J mice were fed for 3 months with either: i) control diet, ii) HF, iii) HF+100 mg/kg dMF, or iv) HF+300 mg/kg dMF. Body weight gain, food intake, visceral fat accumulation, fasting blood glucose, plasma lipids, and aortic, heart, and liver histopathologies were evaluated. Adipocyte lipid accumulation, autophagy, and bile acid binding were also investigated. Results and conclusion HF increased food intake, body weight, visceral fat, plasma total cholesterol (TC) and low-density lipoprotein (LDL), TC/HDL ratio, blood glucose, aortic collagen, arterial and cardiac wall thickness, and liver lipid. Both dMF doses prevented hyperphagia, body weight gain, and visceral fat accumulation, lowered blood glucose, plasma TG and unfavourable TC/HDL and elevated plasma HDL beyond baseline. Arterial and cardiac wall hypertrophy, aortic collagen fibre accumulation and liver lipid deposition ameliorated in dMF-fed mice. Clinical trials on dMF are worthwhile but outcomes should be holistic commensurate with the constellation of disease risks. Here, dMF should supplement the switch to nutrient-rich from current energy-dense diets that are progressively crippling national health systems., Graphical abstract Image 1, Highlights • Uses modest amounts of whole unextracted mulberries as in traditional medicine. • Studies important pathologies found in metabolic syndrome, the number one challenge to modern medicine. • dMF reduces risk factors associated with dyslipidemia. • dMF prevents weight gain, visceral fat and hepatic fat accumulation with fatty diets. • dMF ameliorates vascular fibrosis and cardiac hypertrophy, thereby preventing myocardial infarction risk.
- Published
- 2021