Search

The goal of this study was to detect the risk of developing different atopic dermatitis (AD) phenotypes in children (isolated or combined with other comorbid atopic diseases (AtD)) depending on serum concentrations of cutaneous T-cell attracting chemokine (CTACK)/CCL27. The main group comprised 39 children aged 3 to 18 years old suffering from different AD phenotypes – isolated (18 patients) and combined with comorbid AtD – AR/ARC and/or bronchial asthma (21 patients). The control group comprised 47 children aged 3 to 18 years old, suffering from diseases of the gastrointestinal tract (GIT). Serum CTACK/CCL 27 concentrations were detected in all children. In the full main group, the average level of CTACK/CCL27 was significantly higher compared to the patients of the control group: 4403.6 pg/ml (95% CI: 3726.2; 5148.7, p

Background Mycosis fungoides (MF) is the most common form of cutaneous T-cell lymphoma. Interleukin (IL)-32 is a proinflammatory cytokine expressed by activated natural killer cells, T cells, keratinocytes, and fibroblasts. Cutaneous T-cell-attracting chemokine (CTACK/CCL27) is selectively expressed in the skin and attracts CC chemokine receptor 10-expressing skin-homing memory T cells. Objective To investigate the role of IL-32 and CTACK in the pathogenesis of MF by assessing IL-32 and CTACK levels in patients with MF and normal controls. Patients and methods Serum samples and skin biopsies from lesional and nonlesional skin of 16 patients and controls were collected to examine CTACK and IL-32 levels using ELISA, and results were compared with controls. In six patients, we collected the sera before and after treatment. Results A highly significant difference between cases and controls regarding CTACK and IL-32 serum and tissue levels was found in this case–control study. A highly significant difference between CTACK and IL-32 levels in lesional tissue, nonlesional tissue, and normal skin was found. IL-32 and CTACK markers were strongly correlated with the types of skin lesions. In six patients, serum CTACK and IL-32 were significantly decreased after treatment. Conclusion CTACK/CC chemokine receptor 27 and IL-32 may play an important role in the pathophysiology of MF.

Введение. Общий сывороточный иммуноглобулин Е, тимусом и активацией регулируемый хемокин (ТАРХ) и кожный Т-клетки аттрактирующий хемокин (КТАХ) известны как факторы патогенеза атопического дерматита. Тем не менее существует неоднозначность данных относительно ассоциаций данных биомаркеров с клиническими проявлениями вышеупомянутого заболевания. Цель. Выявить взаимосвязь общего иммуноглобулина E, тимусом и активацией регулируемого хемокина и кожного Т-клетки аттрактирующего хемокина с различными фенотипами атопического дерматита у детей отдельно и в сочетании с другими атопическими коморбидными состояниями (сезонным аллергическим риноконъюнктивитом, круглогодичным аллергическим ринитом, бронхиальной астмой). Материалы и методы. Основную группу составили 39 пациентов в возрасте от 3 до 18 лет, страдающих атопическим дерматитом отдельно и с коморбидными атопическими состояниями – сезонным аллергическим риноконъюнктивитом, круглогодичным аллергическим ринитом и бронхиальной астмой. Контрольную группу составили 47 детей в возрасте от 3 до 18 лет, без атопии, с заболеваниями желудочно-кишечного тракта. Пациентам обеих групп проводилось определение сывороточных концентраций вышеупомянутых биомаркеров. Результаты. Были обнаружены достоверно более высокие уровни общего сывороточного иммуноглобулина Е и КТАХ у пациентов с атопией по сравнению с контрольной группой пациентов. Сывороточные уровни ТАРХ не показали достоверных различий между пациентами основной и контрольной групп; тем не менее обнаружена достоверная прямая взаимосвязь со степенью тяжести фенотипов атопического дерматита отдельно и в сочетании с другими атопическими коморбидностями в целом и с клиническим индексом «scoring atopic dermatitis» в частности. Также имелись достоверные обратные ассоциации с возрастом у пациентов основной и контрольной групп. Общий сывороточный иммуноглобулин Е и КТАХ имели достоверные прямые ассоциации со всеми исследованными фенотипами атопического дерматита. Существует сильная перспектива сочетания сывороточного общего IgE, ТАРХ и КТАХ в качестве эффективной панели биомаркеров для оценки интенсивности воспаления при различных фенотипах атопического дерматита. Выводы. Комбинированное использование общего сывороточного иммуноглобулина Е, тимусом и активацией регулируемого хемокина и кожного Т-клеточного аттрактирующего хемокина представляет собой новую перспективную хемокиновую панель для оценки степени тяжести у детей, страдающих различными фенотипами атопического дерматита отдельно и в сочетании с коморбидными атопическими заболеваниями., Introduction. Serum total immune globulin E, thymus and activation-regulated chemokine (CTACK), and cutaneous T-cell attracting chemokine (TARC) are known as contributing to the pathophysiology of atopic dermatitis. Still, there is the data ambiguity regarding the associations of serum biomarkers with the clinical manifestations of the disease. Purpose. To detect the associations of total immune globulin E, thymus and activation regulated chemokine, and cutaneous T-cell attracting chemokine with different phenotypes of atopic dermatitis in children – alone and combined with other atopic comorbidities (seasonal allergic rhino-conjunctivitis, perennial allergic rhinitis, bronchial asthma). Materials and methods. The main group consisted of 39 patients aged from 3 to 18 years suffering from atopic dermatitis alone and with comorbid atopic disorders – seasonal allergic rhino-conjunctivitis, perennial allergic rhinitis, and bronchial asthma. The control group consisted of 47 children aged from 3 to 18 years, non-atopics, suffering from the gastro-intestinal tract disorders. The patients of both groups were tested for the serum concentrations of the above- mentioned serum biomarkers. Results. There were detected significantly higher levels of total serum immune globulin E and CTACK in atopic patients if compared to controls. Serum TARC showed no significant differences between the main and control group; still, it had significant direct associations with the degree of severity of atopic dermatitis phenotypes alone and combined with other atopic disorders in general and with clinical index “scoring atopic dermatitis” in particular. It had also significant indirect associations with age in patients of the main and control groups. Serum total immune globulin E and CTACK had significant direct associations with all the studied atopic dermatitis phenotypes. There is a strong perspective of combining the serum total IgE, TARC and CTACK as the effective biomarker panel for assessing the intensity of inflammation within different atopic dermatitis phenotypes. Conclusions. Combined use of serum total immune globulin E, thymus and activation-regulated chemokine and cutaneous T-cell attracting chemokine is the novel perspective chemokine-based panel for assessing the degree of severity in patients that suffer from different phenotypes of atopic dermatitis alone and combined with comorbid atopic disorders., Педиатрия. Восточная Европа, Выпуск 1 2021, Pages 21-31

PURPOSE OF REVIEW: A large number of studies investigating the correlation between severity of atopic dermatitis and various biomarkers have been published over the past decades. The aim of this review was to identify, evaluate and synthesize the evidence examining the correlation of biomarkers with disease severity in atopic dermatitis patients, something that has not been performed previously. RECENT FINDINGS: Three electronic databases were systematically searched and relevant studies were selected for inclusion. A total of 222 articles, reporting on 115 different biomarkers in 30 063 patients, were critically appraised. Studies were divided into two main groups. The first group consisted of longitudinal randomized controlled trials and cohort studies, which reported measurements at multiple time points. The second contained cross-sectional studies that reported only one measurement per patient. Out of 222 articles, 108 articles reported sufficient data for meta-analysis. Only four biomarkers were eligible for meta-analysis in the longitudinal group, and nine in the cross-sectional group. SUMMARY: Serum thymus and activation-regulated chemokine (TARC) was found to be the most reliable biomarker studied, showing pooled correlation coefficients of 0.60 (95% CI 0.48-0.70) and 0.64 (95% CI 0.57-0.70) in longitudinal and cross-sectional studies, respectively. Additional biomarkers that could prove useful but require additional research include serum cutaneous T-cell attracting chemokine (CTACK), sE-selectin, macrophage-derived chemokine (MDC), lactate dehydrogenase (LDH) and interleukin (IL)-18.