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Multielemental compositions (Ag, As, Ba, Be, Cd, Cs, Co, Cr, Cu, Mo, Ni, Pb, Sb, Se, Sn, Sr, Tl, Rb, V, and Zn) of 106 cowpea bean samples belonging to different varieties collected from the province of Corrientes in Argentina were determined using inductively coupled plasma mass spectrometry (ICP-MS). Based on the multielemental data, five supervised learning techniques, namely, linear discriminant analysis (LDA), partial least square discriminant analysis (PLS-DA), k nearest neighbors (k-NN), random forest (RF), and support vector machine (SVM) with radial basis function Kernel, were computed aiming at building classification models that allow one to predict the botanical variety of the samples based on their element profiles. The best classification performance was obtained by SVM with 93% accuracy rate. The model developed through this method enabled the correct separation of the samples into the five cowpea varieties investigated, where 100% sensitivity was achieved for most of the predicted classes. Thus, SVM was the algorithm selected for the classification of the cowpea beans according to their botanical variety. Multielemental determination coupled with supervised pattern recognition techniques have proved to be an interesting approach for differentiating a diverse range of cowpea genotypes. This study has contributed toward generalizing the use of multielemental fingerprinting as a promising tool for testing the authenticity of cowpea beans on a global scale. Fil: Pérez Rodríguez, Michael. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste. Instituto de Química Básica y Aplicada del Nordeste Argentino. Universidad Nacional del Nordeste. Facultad de Ciencias Exactas Naturales y Agrimensura. Instituto de Química Básica y Aplicada del Nordeste Argentino; Argentina Fil: Gaiad, José Emilio. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste. Instituto de Química Básica y Aplicada del Nordeste Argentino. Universidad Nacional del Nordeste. Facultad de Ciencias Exactas Naturales y Agrimensura. Instituto de Química Básica y Aplicada del Nordeste Argentino; Argentina Fil: Hidalgo, Melisa Jazmin. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste. Instituto de Química Básica y Aplicada del Nordeste Argentino. Universidad Nacional del Nordeste. Facultad de Ciencias Exactas Naturales y Agrimensura. Instituto de Química Básica y Aplicada del Nordeste Argentino; Argentina Fil: Avanza, María Victoria. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste. Instituto de Química Básica y Aplicada del Nordeste Argentino. Universidad Nacional del Nordeste. Facultad de Ciencias Exactas Naturales y Agrimensura. Instituto de Química Básica y Aplicada del Nordeste Argentino; Argentina Fil: Pellerano, Roberto Gerardo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste. Instituto de Química Básica y Aplicada del Nordeste Argentino. Universidad Nacional del Nordeste. Facultad de Ciencias Exactas Naturales y Agrimensura. Instituto de Química Básica y Aplicada del Nordeste Argentino; Argentina

Cowpea bean is a crop of social, economic and nutritional importance of the Colombian Caribbean, which according to some reports, has been affected in its yield, due to environmental changes especially high temperatures, which are increasing with the passing of the times. These fluctuations can cause negative effects on cowpea bean crop pollen viability. This research was carried out at Universidad de Córdoba aiming to evaluate the viability of pollen from three cowpea bean cultivars collected at different hours (6.00, 8: 00, 10: 00 and 12:00) and to determine the effect of different environments (fridge and cold-room, 1,1 ±0,5°C y RH 68% y 5,6 ± 1,0°C y RH 48%),) on pollen viability storaged for 6 and 12 days, which was determined by using staining methods with 1% Acetocarmín and 3% tetrazolium. Pollen was obtained from the collection of flower buds in anthesis in which temperature and relative humidity records were taken at the time of each sampling, using a using a completely randomized experimental design with a factorial arrangement of three factors; 2 (storage conditions), 3 (conservation times) 3 (genotypes), with four repetitions for pollen conservation. Regarding the time of collection, it was used a completely randomized experimental design with a factorial arrangement of two factors; 4 (hours of collection) and 3 (genotypes) for a total of 12 treatments, with four repetitions. The results stated that storage conditions do not impact pollen viability. As a matter of fact, conservation time and each genotype pollen features do influence its viability. With acetocarmin test were obtained the highest percentage of viable pollen (90%), in contrast with tetrazolium test, which was 70% where the pollen collection time did not affect its viability INTRODUCCIÓN ................................................................................................... 15 1. OBJETIVOS ....................................................................................................... 18 1.1. OBJETIVO GENERAL .................................................................................... 18 1.2. OBJETIVOS ESPECÍFICOS ........................................................................... 18 2. HIPÓTESIS ........................................................................................................ 19 3. REVISIÓN DE LITERATURA ............................................................................. 20 3.1. GENERALIDADES DE LA ESPECIE............................................................... 20 3.2. ORIGEN E IMPORTANCIA ECONÓMICA DEL FRIJOL CAUPÍ ......................... 20 3.3. IMPORTANCIA SOCIAL ................................................................................. 21 3.4. FACTORES QUE INCIDEN EN LA FLORACIÓN DEL CULTIVO ....................... 22 3.5. FACTORES QUE AFECTAN LA VIABILIDAD DEL POLEN ................................... 23 3.6. BIOLOGÍA FLORAL ........................................................................................ 24 3.7. MORFOLOGÍA DE LOS GRANOS DE POLEN ............................................... 25 3.8. ALMACENAMIENTO DEL POLEN .................................................................. 26 3.9. ESTUDIOS RELACIONADOS CON LA VIABILIDAD DEL POLEN .................. 27 4. MATERIALES Y MÉTODOS .............................................................................. 29 4.1. LOCALIZACIÓN .............................................................................................. 29 4.2. MATERIALES ................................................................................................. 29 4.3. MÉTODOS ...................................................................................................... 30 4.3.1. Viabilidad del polen en función de la hora de colecta .................................. 30 4.3.2. Efecto del almacenamiento en la viabilidad del polen .................................. 33 5. RESULTADOS Y DISCUSIÓN .......................................................................... 37 5.1. VIABILIDAD DEL POLEN EN FUNCIÓN DE LA HORA DE COLECTA ........... 37 5.2. EFECTO DEL TIEMPO DE ALMACENAMIENTO EN LA VIABILIDAD DEL POLEN ................................................................................................................... 44 6. CONCLUSIONES .............................................................................................. 56 REFERENCIAS ..................................................................................................... 57 El fríjol caupí es un cultivo de importancia social, económica y alimenticia del Caribe colombiano, el cual según reportes se ha visto afectado actualmente en sus rendimientos, por cambios en los factores ambientales, especialmente por altas temperaturas que van en aumento con el pasar de los tiempos; dichas variaciones pueden causar efectos negativos en la viabilidad polínica de estas plantas. La presente investigación se llevó a cabo en la Universidad de Córdoba, la cual tuvo como objetivo evaluar la viabilidad de polen de tres cultivares de frijol caupí, colectado en diferentes horas del día (6:00, 8:00,10:00am y 12:00m) y determinar el efecto de diferentes ambientes nevera y cuarto frio 1,1 ±0,5°C y HR 68% y 5,6 ± 1,0°C y HR 48%), en la viabilidad de polen, conservado por 6 y 12 días, la cual se evaluó, mediante el uso de métodos de tinción con acetocarmín al 1% y sal de tetrazolio al 3%. El polen se obtuvo de la colecta de botones florales en antesis en el cual se tomaron registros de temperatura y humedad relativa al momento de cada muestreo, utilizando un diseño experimental completamente al azar con arreglo factorial de tres factores; 2 (condiciones de almacenamientos), 3 (tiempos de conservación) y 3 (genotipos) con cuatro repeticiones para la conservación del polen, en cuanto a la hora indicada de colecta se utilizó un diseño completamente al azar con arreglo factorial de dos factores 4 (horas de colecta) y 3 (genotipos), para un total de 12 tratamientos, con cuatro repeticiones. Los resultados muestran que las condiciones de almacenamiento no afectaron la viabilidad de polen, por el contrario, el tiempo de conservación y las características del polen de cada genotipo ejercieron influencia en su viabilidad; con la prueba de acetocarmín se obtuvieron los valores más altos para porcentaje de polen viable (90%), en contraste con la prueba de tetrazolio el cual fue de un 70% donde la hora de colecta del polen no afectó su viabilidad. Pregrado Ingeniero(a) Agronómico(a) Trabajo de Investigación y/o Extensión

Submitted by JAFF RIBEIRO DA SILVA (jaff.ribeiro@unesp.br) on 2020-03-05T16:07:13Z No. of bitstreams: 2 TESE Jaff Ribeiro.pdf: 3502619 bytes, checksum: ac6ac40e62223318641d2cf50efcd3e1 (MD5) Termos de sigilo Jaff Ribeiro.pdf: 7952582 bytes, checksum: 5adc0a2ac3d36f729d21ea97852ecfa3 (MD5) Approved for entry into archive by Ana Carolina Gonçalves Bet null (abet@iq.unesp.br) on 2020-03-11T14:17:31Z (GMT) No. of bitstreams: 1 Termos de sigilo Jaff Ribeiroda Silva.pdf: 8000373 bytes, checksum: fb5ab4529885b19c17d08c6cae0693d3 (MD5) Made available in DSpace on 2020-03-11T14:17:34Z (GMT). No. of bitstreams: 1 Termos de sigilo Jaff Ribeiroda Silva.pdf: 8000373 bytes, checksum: fb5ab4529885b19c17d08c6cae0693d3 (MD5) Previous issue date: 2020-02-14 Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Evaluation of resistance of Vigna unguiculata L. landraces, used as green beans, to Aphis craccivora Koch was tested. Eleven landraces and standard genotypes were used in a free choice protocol: BR 17-Gurguéia, VITA 7 (susceptible), BRS Guariba and TVu 408P2 (resistant). A completely randomized blocks design was used with six replicates. Seedlings were infested with five six-day-old adult insects. The number of alive adults after 48 h and of alive nymphs after 96 h was evaluated. The results evidenced that there are high genetic diversity among the landraces, the antibiosis as the main mechanism of resistance. By means of multivariate analysis we suggest crosses among the commercial cultivar BRS Guariba and the most dissimilar varieties CCE-088, CCE-075, CCE-013 and CCE-062 to obtain superior hybrids.

Submitted by Jorge Silva (jorgelmsilva@ufrrj.br) on 2017-10-04T17:35:36Z No. of bitstreams: 1 2013 - Fernanda Santana de Paulo.pdf: 1349643 bytes, checksum: 8da583439baf6dcac85c9ddb6af5ffbc (MD5) Made available in DSpace on 2017-10-04T17:35:36Z (GMT). No. of bitstreams: 1 2013 - Fernanda Santana de Paulo.pdf: 1349643 bytes, checksum: 8da583439baf6dcac85c9ddb6af5ffbc (MD5) Previous issue date: 2013-02-27 CAPES Embrapa The cowpea bean is a legume that has a wide economic potential, but it has not been explored efficiently. For this reason it has shown a yield potential far from the expectations of a so efficient legume in NBF as can be. The objective of this study is to obtain appropriate levels of P, Co, Mo nutrients. It is believed that these nutrients can optimize nodulation, nitrogen biological fixation and the growth of cowpea bean plants in representative soils from Cerrado, Mato Grosso. Based on the chemical analysis from the soil cited before, calculations for fertilization and liming were made. From the results obtained, were established doses for the elements of the present work (P, Co and Mo). The experiment was conducted in a greenhouse conditions and performed in a randomized block design. It was used a factorial arrangement 2 x 5 (2 soils; five doses of each element of the present study ? inoculated with 3262 strain) + 1 + 3 replicates. The additional treatment (+1) was included to evaluate crop performance when applied 50 Kg.ha-1 of nitrogen. The study was split in two trials. In the first trial, different dosages of phosphorous were tested: control (without Phosphorus application); low (44 mg.vase-1 of P), medium (88 mg.vase-1 of P), medium-low (130mg.vase-1 of P) and high (175 mg.vaso-1 of P). These tests were made to obtain a optimum dosage. In the second trial, cobalt and molybdenum nutrient was tested as the following levels respectively: 0, 2, 3, 4, 6 mg and 0, 8, 16, 32 and 64 mg. In the thirty-fifth day, the experiment developed in a greenhouse was collected. From the plants were evaluated height and plant diameter, nodulation (dry matter and number of nodules), shoot dry matter, root dry matter, chlorophyll content and N accumulation in shoot. The 88mg of P.vase-1 dosage that corresponds to 200 kg.ha-1 of P2O5 and the medium-high Cobalt and Molybdenum dosage that corresponds the application of 32g of Mo.ha-1 + 4g.ha-1 of Co provided higher nodulation in cowpea bean plants with consequent increase of biomass and nitrogen accumulation. The site where soil was collected had influence on the development and on the nodulation of the cowpea bean crop, when submitted to different dosages of P and Co+Mo. O feij?o-caupi ? uma leguminosa de vasto potencial econ?mico, por?m n?o est? sendo explorado de maneira eficiente apresentando-se com potencial produtivo bem aqu?m das expectativas de uma leguminosa t?o eficiente em FBN. Sendo ent?o, o objetivo deste trabalho obter os n?veis adequados dos nutrientes P, Co e Mo que otimizem a nodula??o, a fixa??o biol?gica de nitrog?nio e o crescimento de plantas de feij?o-caupi em solos representativos do Cerrado do Mato Grosso. Com base na an?lise qu?mica do solo, foram realizados os c?lculos da necessidade de calagem e aduba??o e foram estabelecidas as doses dos elementos em estudo (P, Co e Mo). O experimento foi realizado em condi??es de casa de vegeta??o. O delineamento experimental foi em blocos ao acaso em esquema fatorial 2 x 5 (2 solos; 5 doses de cada elemento de estudo ? inoculadas com a estirpe 3262) +1 e 3 repeti??es. O tratamento adicional (+1) foi inclu?do para avaliar o desempenho da cultura na dose de N proporcional a 50 kg.ha-1. O trabalho foi dividido em dois ensaios, onde primeiro foram testadas dosagens diferenciadas de f?sforo que foram denominadas: controle, (sem aplica??o de F?sforo); baixa (44 mg.vaso-1 de P), m?dia (88 mg.vaso-1 de P), m?dia-alta (130 mg.vaso-1 de P), alta (175 mg.vaso-1 de P) para a obten??o de uma dosagem ?tima. Na segunda fase, foram testados os micronutrientes Co nas doses: 0, 2,3 ,4 ,6 mg e Mo nas doses: 0, 8, 16, 32 e 64 mg. Em casa de vegeta??o, aos 35 dias foi feita a coleta, as vari?veis avaliadas foram: altura e di?metro da planta, nodula??o (massa de n?dulos secos e n?mero de n?dulos), massa seca da parte a?rea, massa seca da raiz, teor de clorofila e ac?mulo de N na parte a?rea. A dose de 88 mg de P. vaso-1, que corresponde a 200 kg.ha-1de P2O5 e at? a dose m?dia-alta de cobalto e molibd?nio com correspondente a aplica??o de 32 g de Mo. ha-1 + 4 g ha-1de Co proporcionou maior nodula??o das plantas de feij?o-caupi com consequente aumento da biomassa do vegetal e ac?mulo de nitrog?nio. O local de coleta de solo influenciou no desenvolvimento e nodula??o da cultura do feij?o-caupi, quando submetida a diferentes doses de P e Co+Mo.

El desarrollo de nuevas formulaciones de rizobios con materiales poliméricos mediante la aplicación de técnicas de recubrimiento, brinda grandes ventajas como la de controlar la liberación del ingrediente activo, ofrecer protección frente a las condiciones ambientales; además de permitir mantener viables las células microbianas sin la pérdida de las capacidades metabólicas y fisiológicas. Por tal razón, se propuso desarrollar un prototipo de formulación sólido, utilizando polímeros como vehículo para evaluar su efecto sobre la actividad biológica entre la simbiosis de Rhizobium sp. y fríjol cowpea en invernadero. Se realizaron inicialmente diversos estudios para la caracterización molecular de la bacteria, así como los estudios de preformulación para analizar la influencia de factores fisicoquímicos y el comportamiento de los polímeros sobre la bacteria. Se evidenció que la cepa G58, pertenece al género Rhizobium, lo anterior se realizó mediante la identificación molecular a través de la amplificación del fragmento 16S rDNA presentando un 99% de similitud. A su vez, se demostró un amplio rango de tolerancia de la bacteria a diferentes pHs, temperatura y fuentes de carbono. De igual forma, se estableció mediante el test de Tukey (P0,05) la influencia negativa del polietilenglicol, carbómero y alcohol polivinílico sobre la viabilidad celular del microorganismo; obteniendo mejores resultados cuando fueron empleados dos polímeros de alginato de sodio y la hidroxipropilmetilcelulosa. De un total de ocho polímeros estudiados sólo los tres anteriormente citados fueron seleccionados y a los cuales se les determinó la capacidad de formación de película, la permeabilidad y la capacidad de hinchamiento como aspectos adicionales en la caracterización de los materiales poliméricos. Mediante la utilización de la técnica de lecho fluidizado, se obtuvo los prototipos de formulaciones sólidas y se cuantificó las actividades de promoción de crecimiento sin presentar efectos negativos sobre sus capacidades biológicas. Una vez realizados estos estudios, se procedió a evaluar mediante dos experimentos bajo invernadero el efecto de las preparaciones poliméricas y los prototipos de formulación sólidos, sobre la actividad simbiótica entre la cepa G58 y la planta de fríjol cowpea [Vigna unguiculata (L.) Walp]. Finalmente, los resultados obtenidos mediante un análisis multivariado de componentes principales mostraron que la aplicación de los polímeros bajo las dos presentaciones no presentaron efectos negativos sobre la actividad de fijación biológica de nitrógeno, mostrando mejores respuestas en los prototipos de formulaciones sólidas, debido al proceso de recubrimiento, el cual permitió controlar la liberación de la bacteria y cederla adecuadamente. / Abstract. Development of new formulations of rhizobia with polymeric materials by implementing coating techniques, provides great advantages because firstly controls the release of the active ingredient, offering protection against environmental conditions and likewise, allow to maintain viable microbial cells without loss of metabolic and physiological capabilities. For this reason, was proposed to develop a solid formulation prototype using polymers as vehicles to evaluate their effect on biological activity between the symbiosis Rhizobium sp.-cowpea in the greenhouse. Was initially established several studies on molecular characterization of the bacteria and preformulation of studies to analyze the influence physicochemical factors and the behavior of polymers on the bacteria. It showed that strain G58, belongs to the genus Rhizobium sp., the above was performed by amplification of 16S rDNA fragment showing a 99% similarity. In turn, was demonstrated a wide range of tolerance of the bacterium at different pHs, temperature and carbon sources. Similarly, was established through the Tukey test (P 0.05) the negative influence of polyethylenglycol, carbomer and polyvinyl alcohol on cellular viability of the microorganism; showing better results when the two polymers used sodium alginate and hydroxypropylmethylcellulose. Therefore, from a total of eight polymers were selected these last three, to which they studied the ability of film formation, permeability and swelling as additional aspects in the characterization of polymeric materials. In addition to this, was obtained through the fluidized bed, the solid formulation prototypes, at them was quantified the growth promotion activities without submitting negative effects on their biological capabilities. Once these studies were conducted, proceeded to assess by greenhouse experiments two the effect of polymer preparations and solid formulation prototypes on the symbiotic activity between the G58 strain and the cowpea plant [Vigna unguiculata (L.) Walp]. Finally, the results obtained by multivariate analysis of principal components showed that the application of polymers in the two presentations had no negative effects on the activity of biological nitrogen fixation, showing responses better in the prototypes of solid formulations, due to the coating process, which allowed control the release of the bacteria and transfer it appropriately. Maestría

Submitted by (lucia.rodrigues@ufrpe.br) on 2016-08-10T13:22:32Z No. of bitstreams: 1 Maria de Fatima da Silva.pdf: 619107 bytes, checksum: 566cd7dafe2052de47a3a66eb87eda27 (MD5) Made available in DSpace on 2016-08-10T13:22:32Z (GMT). No. of bitstreams: 1 Maria de Fatima da Silva.pdf: 619107 bytes, checksum: 566cd7dafe2052de47a3a66eb87eda27 (MD5) Previous issue date: 2011-02-28 Conselho Nacional de Pesquisa e Desenvolvimento Científico e Tecnológico - CNPq Cowpea (Vigna unguiculata (L.) Walp) in Brazil is grown in the most different climatic conditions. By the adaptability to tropical conditions and recognized as a culture of high socioeconomic value, this legume has an important role for the capitalization of small farmers in the Northeast region. Cowpea may obtain adequate quantities of N by the process of biological nitrogen fixation (FBN) when associated with specific and effective bacteria. However, in Brazil the practice of inoculation is not widely processed due to the low agricultural technology and inconsistent responses using the seed inoculation process. The main goal of this study was to evaluate the effect of different rates of inoculum on effectivity and competitiveness of Bradyrhizobium (strain BR 3267) on cowpea grown in greenhouse conditions. The first experiment was carried out with five different concentration of strain BR 3267 (rhizobia cells/mL of inoculant), N mineral fertilizer treatment without inoculation, and a control without inoculation and N mineral fertilizer. The second experiment conducted in Leonard jars with inoculant produced with BR 3267 strain, 15 native rhizobia (isolated from the used soil), and a control treatment without inoculation and no N mineral fertilizer applied. The application of the low recommended rate increased number and dry biomass of nodules, shoot dry biomass and shoot N total uptake. In the Leonard jars experiment the rhizobia strain BR 3267 was more effective on nitrogen fixation and showed greater competitiveness compared to the native rhizobia isolates. The BRS Pujante cultivar was benefit by the FBN process when inoculated with strain BR 3267 proportional to the inoculum rate. O feijão - caupi (Vigna unguiculata (L.) Walp.) é cultivado, no Brasil, nas mais diferentes condições edafoclimáticas. Devido a sua boa adaptabilidade às condições tropicais e por ser uma cultura de alto valor sócio-econômico, tem um papel importante para a capitalização de pequenos agricultores na região Nordeste. Por ser uma leguminosa, o feijão-caupi pode adquirir N em quantidades adequadas para suprir suas necessidades através do processo de fixação biológica do nitrogênio (FBN) quando associado com bactérias específicas e efetivas. Entretanto a prática da inoculação não é muito utilizada devido ao baixo aporte tecnológico nas aéreas de cultivo e respostas inconsistentes com os inoculantes utilizados. O objetivo do presente estudo foi de avaliar o efeito da aplicação de diferentes taxas de inóculo, habilidade competitiva e eficiência para a FBN da estirpe de Bradyrhizobium sp. BR 3267 na cultura do feijão-caupi em casa de vegetação. No primeiro experimento, conduzido em vasos com solo foram usados sete tratamentos, compreendendo cinco concentrações de células da estirpe BR 3267 (células/mL de inoculante), um tratamento sem inoculação e com adição de fertilizante nitrogenado, e um controle sem inoculação e sem adição de fertilizante nitrogenado. O segundo experimento constituiu de 17 tratamentos, compostos por 15 estirpes nativas, a estirpe BR 3267 e um tratamento sem inoculação e sem adição de nitrogênio, em vasos de Leonard com substrato estéril. A aplicação da dose mínima recomendada incrementou número e biomassa seca dos nódulos, biomassa seca da parte aérea e o acúmulo de nitrogênio na parte aérea. Em substrato estéril a população rizobiana nativa não foi mais eficiente quando comparada à estirpe BR 3267. A estirpe BR 3267 foi mais competitiva e eficiência em relação à população rizobiana nativa. A cultivar BRS Pujante foi mais beneficiada pela FBN quando inoculada com a estirpe BR 3267, proporcionalmente à taxa de inóculo.

The cowpea culture has a huge alimentary importance; it is one of the most cultivated by the rural zone population of North/Northeastern of Brazil. Abiotic stress such as droughts can reduce the crop yield. Therefore, the identification and understanding of the drought tolerance mechanisms are fundamental when selecting cultivars more tolerant to water deficit. This paperwork had as objective the study of ecophysiological and biochemical responses showed by the cultivation of cowpea ´BRS-xiquexique´ when submitted to different water regimes. The studies of the ecophysilogical responses and biochemical analyses were realized in the agricultural greenhouse and in the Botany Department of Biology applied laboratory at the Federal University of Sergipe, located in São Cristóvão-SE. For the cultivation were used vases with 10 litres containing soil composed of vegetal land and washed sand in a proportion of 2:1. There were sown 4 seeds by vase but after 15 days the 2 more vigorous were chosen. By the 46th day of cultivation the irrigation was suspended. The trial design was made with three treatments and five repetitions (T0 = 100% repositioning of the daily lost water by the plant, T1 = 50% repositioning of the daily lost water by the plant, T2 = 25% repositioning of the daily lost water by the plant). Always in the interval of 8am to 8:30am the vases were weighed and the water lost in the last 24 hours was the answer for the established proportions for each treatment. During the experiments, ecophysiological analyses were done every two days and the biochemical analyses, every four days. The ecophysiological studied variables were transpiration (E), stomatal conductance (gs), liquid photosynthesis (A), leaf water potential (ψ), CO2 concentration (Ci) and total chlorophyll. Establishing the ratio of photosynthesis and transpiration it was determined the efficiency of water use (A/E). For the biochemical studies, the variables were the soluble sugar content, level of proline, level of proteins, level of chlorophylls a and b. Every cophsyological and biochemical data was submitted to variance analyses and the averages were compared through Tukey test, by the level of 5% significance with the aid of the statistical programme GraphPad Prism. Version 4.0. Through the results analysis it was possible to identify correlation between water abolition and the ecophysiological responses presented. There was decline in the following values: water potential, transpiration rate, stomatal conductance, liquid photosynthesis and efficient use of water. There was increase in: internal concentration of CO2 and leaf temperature. For the stomatal conductance there was no significant differences between the plants of the treatments T1 and T2, however, there were differences among them and treatment T0 (witness). For the other variables, it could be observed important differences even though in isolated days. Concerning to the biochemical responses, in general there were no changes in levels of the studied variables, susceptible to correlation to the water disability. A cultura do feijão-caupi é de grande importância alimentar, destacando-se como uma das mais cultivadas pelas populações da zona rural do Norte/Nordeste do Brasil. Estresses abióticos como a seca podem reduzir os rendimentos das lavouras. Portanto, a identificação e a compreensão dos mecanismos de tolerância à seca são fundamentais para a seleção de cultivares mais tolerantes ao déficit hídrico. Este trabalho teve como objetivo o estudo das respostas ecofisiológicas e bioquímicas apresentadas pela cultivar de feijão-caupi BRSXiquexique quando submetida a diferentes regimes hídricos. Os estudos das respostas ecofisiológicas e bioquímicas foram conduzidos na estufa agrícola e no Laboratório de Botânica Aplicada do Departamento de Biologia da Universidade Federal de Sergipe, localizada no município de São Cristóvão-SE. Para o cultivo foram usados vasos com capacidade de 10 litros contendo solo composto de terra vegetal e arei lavada na proporção de 2:1. Foram semeadas 4 sementes por vaso e após 15 dias foi feito desbaste deixando-se apena as duas plantas mais desenvolvidas. O cultivo seguiu em condições semi-controladas até o 46º dia quando foram suspensas a irrigações. O delineamento experimental foi inteiramente casualizado com três tratamentos e cinco repetições (T0 = reposição de 100% da quantidade de água perdida diariamente pela planta; T1 = reposição de 50% da quantidade de água perdida diariamente pela planta e T2 = reposição de 25% da quantidade de água perdida diariamente pela planta). Sempre das 08h00min às 08h30min os vasos foram pesados e reposta a água perdida nas 24 horas anteriores nas proporções estabelecidas por cada tratamento. Durante os experimentos foram feitas análises ecofisiológicas, a cada dois dias, e bioquímicas, a cada quatro dias. As variáveis ecofisiológicas estudadas foram transpiração (E), condutância estomática (gs), fotossíntese líquida (A), potencial hídrico foliar (Ψ), concentração interna de CO2 (Ci) e clorofila total. Estabelecendo a razão entre fotossíntese e transpiração determinou-se a eficiência do uso da água (A/E). Já as variáveis bioquímicas estudadas foram teor de açúcares solúveis, teor de prolina, teor de proteína e teores das clorofilas a e b. Todos os dados ecofisiológicos e bioquímicos obtidos foram submetidos à análise de variância e as médias foram comparadas mediante teste de Tukey, ao nível de 5% de significância com auxílio do programa estatístico GraphPad Prism. Versão 4.0. Por meio das análises dos resultados foi possível identificar correlação entre a supressão hídrica e as respostas ecofisiológicas apresentadas. Houve quedas nos potenciais hídricos, nas taxas de transpiração, condutância estomática, fotossíntese líquida, uso eficiente da água e aumentos nas concentrações internas de CO2 e nas temperaturas foliares. Não houve diferenças significativas entres as plantas dos tratamentos T1 e T2 para a variável Condutância estomática enquanto que nas outras puderam ser observadas diferenças mesmo que em dias isolados. Em relação às respostas bioquímicas no geral não houve mudanças nos teores das variáveis estudadas passíveis de correlação com a deficiência hídrica.