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【Objective】The study aimed to clarify the types of pathogens causing anthracnose on Shatian lemon, with a view to providing guidance for the prevention and control of anthracnose on Shatian lemon in field.【Method】The collected anthracnose samples were isolated and purified, and 9 strains of the pathogens ST2-1, ST2-2, ST2-3, ST3-1, ST3-2, ST3-3, ST4-1, ST4-1, ST4-2, and ST4-3 of anthracnose on Shatian lemon were obtained. Then puncturing and spraying inoculation methods were used to test the pathogenicity of the above strains, and their ITS, TUB2, ACT, LSU, GAPDH and CAL fragments were cloned for multi-gene joint identification.【Result】ST2-1, ST2-2, ST2-3, ST3-1, ST3-2, ST3-3, ST4-1, ST4-2 and ST4-3 are all highly pathogenic. The biological characteristics and phylogenetic tree indicated that ST2-1, ST2-2 and ST2-3 belonged to Colletotrichum siamense, ST3-1, ST3-2 and ST3-3 belonged to Colletotrichum cliviicola, ST4-1, ST4-2 and ST4-3 belonged to Colletotrichum fructicola.【Conclusion】Based on the morphological characteristics and molecular biological identification results, the pathogens causing anthracnose on Shatian lemon in Guangzhou were C.siamense, C.cliviicola and C. fructicola, in which C. siamese was isolated from the diseased fruit of Shatian lemon, C. cliviicola and C. fructicola were isolated from the diseased leaves of Shatian lemon, and it was the first report in the world that C. cliviicola could cause anthracnose on citrus plants.

[Objective] The study aimed to clarify the types of pathogens causing anthracnose on Shatian lemon, with a view to providing guidance for the prevention and control of anthracnose on Shatian lemon in field. [Method] The collected anthracnose samples were isolated and purified, and 9 strains of the pathogens ST2-1, ST2-2, ST2-3, ST3-1, ST3-2, ST3-3, ST4-1, ST4-1, ST4-2,and ST4-3 of anthracnose on Shatian lemon were obtained. Then puncturing and spraying inoculation methods were used to test the pathogenicity of the above strains, and their ITS, TUB2, ACT, LSU, GAPDH and CAL fragments were cloned for multi-gene joint identification. [Result] ST2-1, ST2-2, ST2-3, ST3-1, ST3-2, ST3-3, ST4- 1,ST4-2 and ST4-3 are all highly pathogenic. The biological characteristics and phylogenetic tree indicated that ST2-1, ST2- 2 and ST2-3 belonged to Colletotrichum siamense, ST3-1, ST3-2 and ST3-3 belonged to Colletotrichum cliviicola, ST4- 1, ST4-2 and ST4-3 belonged to Colletotrichum fructicola.[Conclusion] Based on the morphological characteristics and molecular biological identification results, the pathogens causing anthracnose on Shatian lemon in Guangzhou were C. siamense, C. cliviicola and C. fructicola, in which C. siamese was isolated from the diseased fruit of Shatian lemon, C. cliviicola and C. fructicola were isolated from the diseased leaves of Shatian lemon, and it was the first report in the world that C. cliviicola could cause anthracnose on citrus plants. [ABSTRACT FROM AUTHOR]

Tilia tomentosa Moench (silver linden) is a globally preferred option for landscaping and street tree planting. Its intentional introduction to China in recent years has led to widespread cultivation. In 2021, a leaf spot disease significantly impacted silver linden trees in a sentinel garden in China, with an average incidence and disease severity of 94.3% and 5.0, respectively, adversely affecting the trees' growth and development. In this investigation, potential fungal pathogens were isolated from the affected leaves, and Koch's postulate was subsequently employed to validate the pathogenicity of the fungal isolates (TM-1 and TM-2). The pathogen was unequivocally identified as Colletotrichum cliviicola through a combination of morphological observation and a multi-locus analysis (ITS, ACT , ApMat , CAL , CHS-1 , GAPDH , and TUB2). This marks the first record of C. cliviicola affecting silver linden in China. The information provided a valuable foundation for the rapid detection and effective prevention and control of the diseases affecting linden trees. • The introduced silver linden in China was affected by leaf spot disease. • Pathogen was identified as Colletotrichum cliviicola. • First report of C. cliviicola causes silver linden leaf spot in China. • Sentinel garden provide early warning information on pathogens to exporting countries. [ABSTRACT FROM AUTHOR]