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2 results on '"clathrin-mediated endo cytosis"'

1. Cuprous oxide nanoparticle-inhibited melanoma progress by targeting melanoma stem cells

Author

Ye Wang, Bin Yu, Ji Zhu, Xinlu Yu, Changcheng Liu, Haiying Zhu, Jingqiang Wang, Chen Wang, Fei Chen, and Hongxia Zhang

Subjects
0301 basic medicine, cancer stem cells, Pharmaceutical Science, Apoptosis, Mice, SCID, medicine.disease_cause, Mice, International Journal of Nanomedicine, Mice, Inbred NOD, clathrin-mediated endo cytosis, Drug Discovery, Original Research, CONPs, Chemistry, Melanoma, General Medicine, Microphthalmia-associated transcription factor, Mitochondria, WM266-4 cell line, Neoplastic Stem Cells, Stem cell, Cell Survival, cuprous oxide nanoparticles, Biophysics, Bioengineering, Antineoplastic Agents, Nerve Tissue Proteins, Receptors, Nerve Growth Factor, Biomaterials, 03 medical and health sciences, Cancer stem cell, Cell Line, Tumor, A375 cell line, medicine, melanoma, SOX10, Animals, Humans, Viability assay, MITF, Organic Chemistry, medicine.disease, Xenograft Model Antitumor Assays, 030104 developmental biology, Tumor progression, Cancer research, Nanoparticles, Carcinogenesis, Copper
Abstract

Bin Yu,1,2,* Ye Wang,3,* Xinlu Yu,1,* Hongxia Zhang,1 Ji Zhu,4 Chen Wang,1 Fei Chen,1 Changcheng Liu,1 Jingqiang Wang,2 Haiying Zhu1 1Department of Cell Biology, Second Military Medical University, 2State Key Laboratory of Genetic Engineering and Collaborative Innovation Center of Genetics and Development, Ministry of Education Key Laboratory of Contemporary Anthropology, Department of Genetics and Development, School of Life Sciences, Fudan University, 3Department of Urinary Surgery, 4Department of Plastic Surgery, Changhai Hospital, Second Military Medical University, Shanghai, People’s Republic of China*These authors contributed equally to this work Abstract:Recent studies have shown that metal and metal oxide have a potential function in anti-tumor therapy. Our previous studies demonstrated that cuprous oxide nanoparticles (CONPs) not only selectively induce apoptosis of tumor cells in vitro but also inhibit the growth and metastasis of melanoma by targeting mitochondria with little hepatic and renal toxicities in mice. As a further study, our current research revealed that CONPs induced apoptosis of human melanoma stem cells (CD271+/high cells) in A375 and WM266-4 melanoma cell lines and could significantly suppress the expression of MITF, SOX10 and CD271 involved in the stemness maintenance and tumorigenesis of melanoma stem cells. CD271+/high cells could accumulate more CONPs than CD271-/low through clathrin-mediated endocytosis. In addition, lower dosage of CONPs exhibited good anti-melanoma effect by decreasing the cell viability, stemness and tumorigenesis of A375 and WM266-4 cells through reducing the expression of SOX10, MITF, CD271 and genes in MAPK pathway involved in tumor progression. Finally, CONPs obviously suppressed the growth of human melanoma in tumor-bearing nonobese diabetic-severe combined immunodeficiency (NOD-SCID) mice, accompanied with tumors structural necrosis and fibrosis remarkably and decreased expression of CD271, SOX10 and MITF. These results above proved the effectiveness of CONPs in inhibiting melanoma progress through multiple pathways, especially through targeting melanoma stem cells.Keywords: cuprous oxide nanoparticles, CONPs, melanoma, cancer stem cells, A375 cell line, WM266-4 cell line, MITF, SOX10, clathrin-mediated endo­cytosis

Published
2017

2. Cuprous oxide nanoparticle-inhibited melanoma progress by targeting melanoma stem cells.

Author

Yu B, Wang Y, Yu X, Zhang H, Zhu J, Wang C, Chen F, Liu C, Wang J, and Zhu H

Subjects
Animals, Antineoplastic Agents administration & dosage, Apoptosis drug effects, Cell Line, Tumor, Cell Survival drug effects, Copper administration & dosage, Humans, Melanoma pathology, Mice, Mice, Inbred NOD, Mice, SCID, Mitochondria drug effects, Mitochondria metabolism, Nanoparticles chemistry, Neoplastic Stem Cells pathology, Nerve Tissue Proteins metabolism, Receptors, Nerve Growth Factor metabolism, Xenograft Model Antitumor Assays, Antineoplastic Agents pharmacology, Copper pharmacology, Melanoma drug therapy, Nanoparticles administration & dosage, Neoplastic Stem Cells drug effects
Abstract

Recent studies have shown that metal and metal oxide have a potential function in antitumor therapy. Our previous studies demonstrated that cuprous oxide nanoparticles (CONPs) not only selectively induce apoptosis of tumor cells in vitro but also inhibit the growth and metastasis of melanoma by targeting mitochondria with little hepatic and renal toxicities in mice. As a further study, our current research revealed that CONPs induced apoptosis of human melanoma stem cells (CD271 +/high cells) in A375 and WM266-4 melanoma cell lines and could significantly suppress the expression of MITF, SOX10 and CD271 involved in the stemness maintenance and tumorigenesis of melanoma stem cells. CD271 +/high cells could accumulate more CONPs than CD271 -/low through clathrin-mediated endocytosis. In addition, lower dosage of CONPs exhibited good anti-melanoma effect by decreasing the cell viability, stemness and tumorigenesis of A375 and WM266-4 cells through reducing the expression of SOX10, MITF, CD271 and genes in MAPK pathway involved in tumor progression. Finally, CONPs obviously suppressed the growth of human melanoma in tumor-bearing nonobese diabetic-severe combined immunodeficiency (NOD-SCID) mice, accompanied with tumors structural necrosis and fibrosis remarkably and decreased expression of CD271, SOX10 and MITF. These results above proved the effectiveness of CONPs in inhibiting melanoma progress through multiple pathways, especially through targeting melanoma stem cells., Competing Interests: Disclosure The authors report no conflicts of interest in this work.

Published
2017
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