1. Investigation the Possibility of Using Peptides with a Helical Repeating Pattern of Hydro-Phobic and Hydrophilic Residues to Inhibit IL-10
- Author
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Bin Zhu, Scott F. Cummins, Shelley F. Walton, Ming Q. Wei, Kate E. Mounsey, Zhixiu Li, Yaoqi Zhou, Tianfang Wang, Xiaosong Liu, Guoying Ni, Yuedong Yang, Shu Chen, Yuejian Wang, Jian Zhan, and Faculty of Health, School of Biomedical Sciences
- Subjects
0301 basic medicine ,chronic viral and bacterial infections ,Physiology ,Protein Conformation ,Papillomavirus E7 Proteins ,lcsh:Medicine ,Interleukin 10 (IL-10) ,Peptide ,hydro-phobic ,Biochemistry ,White Blood Cells ,Mice ,Protein structure ,Animal Cells ,Immune Physiology ,Medicine and Health Sciences ,Cytotoxic T cell ,Public and Occupational Health ,Enzyme-Linked Immunoassays ,lcsh:Science ,Peptide sequence ,chemistry.chemical_classification ,060408 Genomics ,Vaccines ,Human papillomavirus 16 ,Multidisciplinary ,Immune System Proteins ,biology ,T Cells ,Animal Models ,Vaccination and Immunization ,3. Good health ,Interleukin-10 ,Interleukin 10 ,medicine.anatomical_structure ,Vaccines, Subunit ,Female ,Antibody ,Cellular Types ,Research Article ,T cell ,Immune Cells ,Immunology ,Molecular Sequence Data ,060109 Proteomics and Intermolecular Interactions (excl. Medical Proteomics) ,Mouse Models ,Cytotoxic T cells ,Research and Analysis Methods ,Antibodies ,03 medical and health sciences ,Model Organisms ,medicine ,Animals ,Humans ,Secretion ,Amino Acid Sequence ,Papillomavirus Vaccines ,Immunoassays ,Blood Cells ,Sequence Homology, Amino Acid ,lcsh:R ,Papillomavirus Infections ,Biology and Life Sciences ,Proteins ,Cell Biology ,Surface Plasmon Resonance ,Molecular biology ,Mice, Inbred C57BL ,helical repeating pattern ,030104 developmental biology ,chemistry ,Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization ,biology.protein ,Immunologic Techniques ,lcsh:Q ,Immunization ,Preventive Medicine ,Physiological Processes ,Peptides - Abstract
Blockade of IL-10 signalling clears chronic viral and bacterial infections. Immunization together with blockade of IL-10 signalling or relatively low level of IL-10 further enhances viral and bacterial clearance. IL-10 functions through binding to interleukin 10 receptor (IL-10R). Here we showed that peptides P1 and P2 with the hydrophobic and hydrophilic pattern of the IL10R-binding helix in IL-10 could bind with either IL-10R1 or IL-10, and inhibit inflammatory signals with long duration and negligible cytotoxicity in vitro. Furthermore, P2 can enhance antigen specific CD8+ T cell responses in mice induced by the vaccine based on a long peptide of protein E7 in a human papillomavirus type 16.
- Published
- 2016