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Purpose: This study aims to investigate the biological treatment of excreta wastewater to evaluate the effective production of liquid organic biofertilizer (LOB).Method: This research was carried out by the pre-initial mixing process of excreta with cassava dregs, then biological processing with the aeration rate of 4 L/minutes at environmental temperature (28 oC) and pH of 5 for 42 days with two treatments in triplicates: aerobically (T1), and anaerobically (T2) conditions. LOB was analyzed for physical, chemical, and microbiological parameters.Results: We showed that the characteristics of waste solids analysis of LOB were significantly decreased (P

The characteristic of chicken excreta has a very potential as a breeding media for flies and is known for causing odorous pollutants (NH3 emission) from undigested protein and the activities of urease microorganisms. This study is focused on extracting Maja fruit, to quantify marmelosin from different fruit conditions using HPLC, and to determine the biological activity for handling the chicken excreta problems. In this study, the Kirby-Bauer Test was used to observe the antibacterial activity of marmelosin, the NH3 trapping method was used to determine ammonia emission, and the larvae population was determined by the Fly-Grill method. Marmelosin contents in MFE from immature, mature, and fermented fruit condition were 108.65 μg/g; 65.83 μg/g, and 23.02 μg/g, respectively. The increasing level of marmelosin addition to 50, 100, 150, and 200 μg/mL caused the higher diameter of inhibition zone against E. coli (p

The characteristic of chicken excreta has a very potential as a breeding media for flies and is known for causing odorous pollutants (NH3 emission) from undigested protein and the activities of urease microorganisms. This study is focused on extracting Maja fruit, to quantify marmelosin from different fruit conditions using HPLC, and to determine the biological activity for handling the chicken excreta problems. In this study, the Kirby-Bauer Test was used to observe the antibacterial activity of marmelosin, the NH3 trapping method was used to determine ammonia emission, and the larvae population was determined by the Fly-Grill method. Marmelosin contents in MFE from immature, mature, and fermented fruit condition were 108.65 µg/g; 65.83 &#181g/g, and 23.02 µg/g, respectively. The increasing level of marmelosin addition to 50, 100, 150, and 200 µg/mL caused the higher diameter of inhibition zone against E. coli (p<0.05), which were 2.50, 2.90, 5.06, and 7.27 mm, respectively. The increasing level of MFE addition at 5, 7.5, and 10% (v/v) showed a higher inhibition effect on the NH3 emission from the excreta. The addition of MFE up to 10% (v/v) had no significant effect on the total larvae population of flies that existed in the excreta. It can be concluded that the highest marmelosin content was confirmed in the immature fruit condition. The highest antibacterial activity of marmelosin from MFE was shown at the concentration of 200 µg/mL. The application of 10% (v/v) MFE to the excreta gave the highest inhibition of NH3 emission and minimized the average larvae population of flies. [ABSTRACT FROM AUTHOR]

The negative impact of chemical fertilizers use is soil fertility declining. The situation occurs because of biological, physical, and chemical properties of the soil is decreased. Agricultural waste is a large commodity which utilization can still be optimized, for example by using as raw material for organic liquid fertilizer. Agricultural wastes that have good quality as fertilizer include goat manure, chicken excreta, and coconut husk. The utilization of agricultural waste as organic fertilizer is one way of creating sustainable agriculture. This study aims to test the quality of liquid fertilizer (bioculture) of goat manure and chicken excreta by adding various levels of coconut husk. Bioculture is made by anaerobic fermentation for 21 days. The parameters observed included levels of C-organic, N, P, and K bioculture, as well as germination tests to determine the presence of phytotoxins. The data were analyzed using one way ANOVA. The treatment of P5 (5% coconut husk) showed the highest levels of N and P, while the K content was not significantly different from the treatment of P4 (2.5% coconut husk). The germination test showed no phytotoxin activity in bioculture.

Eggs in the infective stage of the chicken nematode Ascaridia galli are often required for in vivo and in vitro studies on this parasite. The reliability of any artificial A. galli infection depends on the viability and embryonation capacity of A. galli eggs. The aim of this study was to determine ideal storage conditions for maximising the viability of A. galli eggs and maintaining viability for the longest period. A 2 × 2 × 3 × 5 factorial experimental design was employed to investigate the effects of storage temperature (4°C or 26°C), storage condition (aerobic or anaerobic), storage medium (water, 0.1 N H 2 SO 4 or 2% formalin) and storage period (4, 8, 12, 16 and 20 weeks). The viability of eggs was assessed after eggs in all treatment groups were held aerobically at 26°C for 2 weeks after the storage period to test embryonation capacity. Based on morphological characteristics, they were categorised as undeveloped, developing, vermiform, embryonated or dead. The maintenance of viability during storage at 4°C was optimal under anaerobic conditions while at 26°C it was optimal under aerobic conditions. Anaerobic conditions at 26°C led to a rapid loss of viability while aerobic conditions at 4°C had a less severe negative effect on maintenance of viability. Egg storage in 0.1 N H 2 SO 4 resulted in a significantly higher viability overall (54.7%) than storage in 2% formalin (49.2%) or water (37.3%) (P < 0.0001). Untreated water was the least favourable storage medium when eggs were stored at 26°C while it was a medium of intermediate quality at 4°C. The viability of A. galli eggs decreased significantly with storage time (P < 0.0001) depending on the other factors. The lowest rate of decline was seen with storage of eggs under anaerobic conditions at 4°C or aerobic conditions at 26°C in 0.1 N H 2 SO4. Eggs in these treatments retained up to 72% of overall viability at 20 weeks with a decline rate of approximately 2% per week with no significant difference between the two. Therefore, this study has clearly revealed opposing aerobic conditions required for prolonged storage of A. galli eggs in the pre-embryonated state at 4°C. It has also identified that 0.1 N H 2 SO4 provides the best preservation against degradation during storage, particularly at 26°C under aerobic conditions. Achieving strictly anaerobic conditions can be difficult to achieve so storage aerobically at 26°C may be preferred for simplicity. • Egg viability was maintained at 4 and 26°C under anaerobic and aerobic conditions, respectively. • Egg storage in 0.1 N H 2 SO 4 resulted in higher overall viability than storage in 2% formalin or water. • Untreated water was the least favourable storage medium when eggs were stored at 26°C. • 0.1 N H 2 SO4 provides the best preservation against degradation during egg storage. [ABSTRACT FROM AUTHOR]