Search

The present work studies biofuel production using an American native species that belongs to the Argemone genus. It is considered a weed, and its presence extends from the southern United States to some areas of South America; the species Argemone pleiacantha, together with other species of the same genus, is known as "chicalote". Oil physical and chemical properties confirm that chicalote oil is an effective raw material for biofuel production, presenting a fatty acid composition similar to that of soybean oil. A biodiesel production study was carried out using two methods of synthesis, conventional and ultrasound-assisted transesterification, employing the same molar ratio and amount of catalyst in both cases. Reaction time and supplied energy during synthesis were compared in batch mode. The results revealed that ultrasound-assisted transesterification has significant advantages over the conventional one in terms of reaction time and energy savings during chicalote oil synthesis to produce fatty acid methyl esters. [ABSTRACT FROM AUTHOR]

Protoplasts are plant cells from which the pectocellulosic cell wall has been removed, thus keeping the plasma membrane intact. For plant secondary metabolites research, this system is a powerful tool to study the metabolites' dynamics inside the cells, such as the subcellular localization of proteins, characterization of gene function, transcription factors involved in metabolite pathways, protein transport machinery, and to perform single-cell omics studies. Due to its lack of a cell wall, better images of the interior of the cell can be obtained compared to the whole tissue. This allows the identification of specific cell types involved in the accumulation of specialized metabolites, such as alkaloids, given their autofluorescence properties. Here is a simplified protocol to obtain protoplasts from leaves and in vitro cell cultures from Argemone mexicana, which produces the pharmacologically important alkaloids berberine and sanguinarine., (© 2024. The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.)