1. Nanotubes impregnated human olfactory bulb neural stem cells promote neuronal differentiation in Trimethyltin-induced neurodegeneration rat model
- Author
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Hany E. Marei, Ahmed A. Elnegiry, Ahmed Abd-Elmaksoud, Carlo Cenciarelli, Amany Farag, Shymaa Rezk, Anwarul Hasan, Adel Zaghloul, Asma Althani, Zeinab Shouman, Nahla Afifi, and Samah Lashen
- Subjects
Male ,0301 basic medicine ,Time Factors ,Physiology ,Clinical Biochemistry ,nanotubes ,Cognition ,0302 clinical medicine ,Neural Stem Cells ,Amyotrophic lateral sclerosis ,Cells, Cultured ,neural stem cells ,Neurons ,carbon nanotubes (CNTs ,Behavior, Animal ,Tissue Scaffolds ,Neurodegeneration ,Neurodegenerative Diseases ,Anatomy ,Olfactory Bulb ,Neural stem cell ,Neuroepithelial cell ,trimethyltin ,Nanomedicine ,Phenotype ,medicine.anatomical_structure ,olfactory bulb ,human olfactory bulb neural stem cells ,Cell type ,Neurogenesis ,Green Fluorescent Proteins ,Biology ,neurodegeneration rat model ,Transfection ,03 medical and health sciences ,Reaction Time ,cellular - based therapy ,medicine ,Animals ,Humans ,Rats, Wistar ,Maze Learning ,Nanotubes, Carbon ,Multiple sclerosis ,Nervous tissue ,Cell Biology ,medicine.disease ,Olfactory bulb ,Disease Models, Animal ,030104 developmental biology ,Microscopy, Fluorescence ,Nerve Degeneration ,Trialkyltin Compounds ,Neuroscience ,030217 neurology & neurosurgery - Abstract
Neural stem cells (NSCs) are multipotent self-renewing cells that could be used in cellular-based therapy for a wide variety of neurodegenerative diseases including Alzheimer's diseases (AD), Parkinson's disease (PD), amyotrophic lateral sclerosis (ALS), and multiple sclerosis (MS). Being multipotent in nature, they are practically capable of giving rise to major cell types of the nervous tissue including neurons, astrocytes and oligodendrocytes. This is in marked contrast to neural progenitor cells which are committed to a specific lineage fate. In previous studies, we have demonstrated the ability of NSCs isolated from human olfactory bulb (OB) to survive, proliferate, differentiate, and restore cognitive and motor deficits associated with AD, and PD rat models, respectively. The use of carbon nanotubes (CNTs) to enhance the survivability and differentiation potential of NSCs following their in vivo engraftment have been recently suggested. Here, in order to assess the ability of CNTs to enhance the therapeutic potential of human OBNSCs for restoring cognitive deficits and neurodegenerative lesions, we co-engrafted CNTs and human OBNSCs in TMT-neurodegeneration rat model. The present study revealed that engrafted human OBNSCS-CNTs restored cognitive deficits, and neurodegenerative changes associated with TMT-induced rat neurodegeneration model. Moreover, the CNTs seemed to provide a support for engrafted OBNSCs, with increasing their tendency to differentiate into neurons rather than into glia cells. The present study indicate the marked ability of CNTs to enhance the therapeutic potential of human OBNSCs which qualify this novel therapeutic paradigm as a promising candidate for cell-based therapy of different neurodegenerative diseases. This article is protected by copyright. All rights reserved
- Published
- 2017