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The process-based xylem formation model is an important tool for understanding the radial growth process of trees and its influencing factors. While numerous xylogenesis models for conifers have been developed, there is a lack of models available for non-coniferous trees. In this study, we present a process-based model designed for xylem formation and ring growth in broad-leaved trees, which we call the Broad-leaved Tree-Ring (BTR) model. Climate factors, including daylength, air temperature, soil moisture and vapor pressure deficit, drive daily xylem cell production (fibers and vessels) and growth (enlargement, wall deposition). The model calculates the total cell area in the simulated zone to determine the annual ring width. The results demonstrate that the BTR model can basically simulate inter-annual variation in ring width and intra-annual changes in vessel and fiber cell formation in Fraxinus mandshurica (ring-porous) and Betula platyphylla (diffuse-porous). The BTR model is a potential tool for understanding how different trees form wood and how climate change influences this process. [ABSTRACT FROM AUTHOR]

An asymmetric pyrene derivative was designed as a dye with D-π-A structure. This dye was synthesized by the following steps (acylation reaction, Friedele Crafts acylation reaction, bromination reaction, Hagiharae Sonogashira cross-coupling reaction, and knoevenagel condensation reactions). The structure of the dye was analyzed and identified by FT-IR and 1H-NMR. The dye-sensitized solar cells were prepared with the dye and TiO2 electrode. Futhermore, the I-V curves and IPCE curves of the dye-sensitized solar cells were investigated detailedly. The result showed that the photoelectric conversion efficiency of the dye-sensitized solar cells reached 33.5% of N719 dye cells. [ABSTRACT FROM AUTHOR]

An ontology for the structured storage, retrieval, and analysis of data on lithium‐ion battery materials and electrode‐to‐cell production is presented. It provides a logical structure that is mapped onto a digital architecture and used to visualize, correlate, and make predictions in battery production, research, and development. Materials and processes are specified using a predetermined terminology; a chain of unit processes (steps) connects raw materials and products (items) of battery cell production. The ontology enables the attachment of analytical methods (characterization methods) to items. Workshops and interviews with experts in battery materials and production processes are conducted to ensure that the structure is conformable both for industrial‐scale and laboratory‐scale data generation and implementation. Raw materials and intermediate products are identified and defined for all steps to the final battery cell. Steps and items are defined based on current standard materials and process chains using terms that are in common use. Alternative structures and the connection of the ontology to other existing ontologies are discussed. The contribution provides a pragmatic, accessible way to unify the storage of materials‐oriented lithium‐ion battery production data. It aids the linkage of such data with domain knowledge and the automation of data analysis in production and research. [ABSTRACT FROM AUTHOR]

Background and Aims Upscaling carbon allocation requires knowledge of the variability at the scales at which data are collected and applied. Trees exhibit different growth rates and timings of wood formation. However, the factors explaining these differences remain undetermined, making samplings and estimations of the growth dynamics a complicated task, habitually based on technical rather than statistical reasons. This study explored the variability in xylem phenology among 159 balsam firs [ Abies balsamea (L.) Mill.]. Methods Wood microcores were collected weekly from April to October 2018 in a natural stand in Quebec, Canada, to detect cambial activity and wood formation timings. We tested spatial autocorrelation, tree size and cell production rates as explanatory variables of xylem phenology. We assessed sample size and margin of error for wood phenology assessment at different confidence levels. Key Results Xylem formation lasted between 40 and 110 d, producing between 12 and 93 cells. No effect of spatial proximity or size of individuals was detected on the timings of xylem phenology. Trees with larger cell production rates showed a longer growing season, starting xylem differentiation earlier and ending later. A sample size of 23 trees produced estimates of xylem phenology at a confidence level of 95 % with a margin of error of 1 week. Conclusions This study highlighted the high variability in the timings of wood formation among trees within an area of 1 km2. The correlation between the number of new xylem cells and the growing season length suggests a close connection between the processes of wood formation and carbon sequestration. However, the causes of the observed differences in xylem phenology remain partially unresolved. We point out the need to carefully consider sample size when assessing xylem phenology to explore the reasons underlying this variability and to allow reliable upscaling of carbon allocation in forests. [ABSTRACT FROM AUTHOR]

Abstract Background The major fleshy tissues of the apple fruit are spatially separable into cortex and pith. These tissues display differential growth during development. Key features of such differential growth, and sink metabolic programs supporting it have not been investigated previously. We hypothesized that differential growth between these fruit tissues is supported by differential sink metabolic programs, particularly during early development. Growth, metabolite concentrations, and transcript abundance of metabolism-related genes were measured to determine characteristics of differential growth and their underlying metabolic programs. Results The cortex displayed > 5-fold higher growth than the pith during early fruit development, indicating that differential growth was established during this period. Further, when resource availability was increased through sink-removal, cortex growth was preferentially enhanced. Greatest diversity in metabolic programs between these tissues was evident during early fruit development. Higher cortex growth during early development was facilitated by increased catabolism of imported carbon (C) resources, sorbitol and sucrose, and the nitrogen (N) resource, asparagine. It was also associated with enhanced primary C metabolism, and C storage as malate and quinate. The pith metabolic program during this period involved limited allocation of C and N to growth, but greater allocation to storage, and enhanced sucrose-sucrose cycling. Conclusions Together, these data indicate that the fruit cortex tissue displays a resource intensive metabolic program during early fruit development. This provides the C backbones, proteins, energy and osmolytes to support its higher growth.

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While human induced pluripotent stem cells (hiPSCs) provide novel prospects for disease-modeling, the high phenotypic variability seen across different lines demands usage of large hiPSC cohorts to decipher the impact of individual genetic variants. Thus, a much higher grade of parallelization, and throughput in the production of hiPSCs is needed, which can only be achieved by implementing automated solutions for cell reprogramming, and hiPSC expansion. Here, we describe the StemCellFactory, an automated, modular platform covering the entire process of hiPSC production, ranging from adult human fibroblast expansion, Sendai virus-based reprogramming to automated isolation, and parallel expansion of hiPSC clones. We have developed a feeder-free, Sendai virus-mediated reprogramming protocol suitable for cell culture processing via a robotic liquid handling unit that delivers footprint-free hiPSCs within 3 weeks with state-of-the-art efficiencies. Evolving hiPSC colonies are automatically detected, harvested, and clonally propagated in 24-well plates. In order to ensure high fidelity performance, we have implemented a high-speed microscope for in-process quality control, and image-based confluence measurements for automated dilution ratio calculation. This confluence-based splitting approach enables parallel, and individual expansion of hiPSCs in 24-well plates or scale-up in 6-well plates across at least 10 passages. Automatically expanded hiPSCs exhibit normal growth characteristics, and show sustained expression of the pluripotency associated stem cell marker TRA-1-60 over at least 5 weeks (10 passages). Our set-up enables automated, user-independent expansion of hiPSCs under fully defined conditions, and could be exploited to generate a large number of hiPSC lines for disease modeling, and drug screening at industrial scale, and quality.

Reactive oxygen species (ROS) can act as signaling molecules involved in the acclimation of plants to various abiotic and biotic stresses. However, it is not clear how the generalized increases in ROS and downstream signaling events that occur in response to stressful conditions are coordinated to modify plant growth and development. Previous studies of maize (Zea mays L.) primary root growth under water deficit stress showed that cell elongation is maintained in the apical region of the growth zone but progressively inhibited further from the apex, and that the rate of cell production is also decreased. It was observed that apoplastic ROS, particularly hydrogen peroxide (H2O2), increased specifically in the apical region of the growth zone under water stress, resulting at least partly from increased oxalate oxidase activity in this region. To assess the function of the increase in apoplastic H2O2 in root growth regulation, transgenic maize lines constitutively expressing a wheat oxalate oxidase were utilized in combination with kinematic growth analysis to examine effects of increased apoplastic H2O2 on the spatial pattern of cell elongation and on cell production in well-watered and water-stressed roots. Effects of H2O2 removal (via scavenger pretreatment) specifically from the apical region of the growth zone were also assessed. The results show that apoplastic H2O2 positively modulates cell production and root elongation under well-watered conditions, whereas the normal increase in apoplastic H2O2 in water-stressed roots is causally related to down-regulation of cell production and root growth inhibition. The effects on cell production were accompanied by changes in spatial profiles of cell elongation and in the length of the growth zone. However, effects on overall cell elongation, as reflected in final cell lengths, were minor. These results reveal a fundamental role of apoplastic H2O2 in regulating cell production and root elongation in both well-watered and water-stressed conditions.

Reactive oxygen species (ROS) can act as signaling molecules involved in the acclimation of plants to various abiotic and biotic stresses. However, it is not clear how the generalized increases in ROS and downstream signaling events that occur in response to stressful conditions are coordinated to modify plant growth and development. Previous studies of maize (Zea mays L.) primary root growth under water deficit stress showed that cell elongation is maintained in the apical region of the growth zone but progressively inhibited further from the apex, and that the rate of cell production is also decreased. It was observed that apoplastic ROS, particularly hydrogen peroxide (H2O2), increased specifically in the apical region of the growth zone under water stress, resulting at least partly from increased oxalate oxidase activity in this region. To assess the function of the increase in apoplastic H2O2 in root growth regulation, transgenic maize lines constitutively expressing a wheat oxalate oxidase were utilized in combination with kinematic growth analysis to examine effects of increased apoplastic H2O2 on the spatial pattern of cell elongation and on cell production in well-watered and water-stressed roots. Effects of H2O2 removal (via scavenger pretreatment) specifically from the apical region of the growth zone were also assessed. The results show that apoplastic H2O2 positively modulates cell production and root elongation under well-watered conditions, whereas the normal increase in apoplastic H2O2 in water-stressed roots is causally related to down-regulation of cell production and root growth inhibition. The effects on cell production were accompanied by changes in spatial profiles of cell elongation and in the length of the growth zone. However, effects on overall cell elongation, as reflected in final cell lengths, were minor. These results reveal a fundamental role of apoplastic H2O2 in regulating cell production and root elongation in both well-watered and water-stressed conditions. [ABSTRACT FROM AUTHOR]

Climate change is expected to be heterogeneous across the world, with high impacts on the Himalayan ecosystems. There is a need to precisely document cambial phenology and wood formation in these regions to better understand climate-growth relationships and how trees face a warming climate. This study describes the dynamics of cambial phenology in pindrow fir (Abies pindrow) along its altitudinal gradient in the Himalaya. The stages of xylem phenology, and the duration and rate of wood formation were assessed from anatomical observations during the growing season from samples collected weekly from three sites at various altitudes (2392–2965 m a.s.l.) over two years. There were significant differences in the duration and rate of cell formation along the altitudinal gradient, which decreased at increasing altitudes. The growing season duration decreased by 5.2 and 3.7 days every 100 m of increase in altitude in 2014 and 2015, respectively, while the rate of cell formation decreased from 0.38 and 0.44 cells /day to 0.29 and 0.34 cells/day in 2014 and 2015, respectively. Cell production decreased from 63.3 and 67.0 cells to 38.3 and 45.2 cells with a decrease of 4.3 and 3.8 cells per 100 m increase in altitude in 2014 and 2015, respectively. The higher precipitation in 2015 increased the growth rate and resulted in a higher xylem production. Our findings give new insights into the dynamics of cambial phenology and help in better understanding of the potential impacts of climate change on tree growth and forest productivity of Himalayan forests. [ABSTRACT FROM AUTHOR]

Major studies on cell production have placed an emphasis on technical factors such as machine order/layout, family part grouping, work-flow sequence, etc. However, there have still been insufficient investigations as to how workers' aptitudes affect their performance in production cells. Intending to find the worker's aptitude closely relating to her/his performance in cell production, we design a laboratory experiment, and administer 11 paper-and-pencil tests of the Japanese General Aptitude Test Battery (GATB) to the workers and measure their seven work-related aptitudes. We pay special attention to detecting outliers in the GATB scores and the cell production experiment results, and then examine the partial correlat ions between the workers' GATB scores and their performance. As the GATB scores can't directly measure a worker's aptitude towards the assembly tasks in production cells, we make use of principal component analysis and obtain a component score that correlates significantly with the workers' performance in cell production. As the result, we can apply this component score to measure a worker's aptitude towards the assembly tasks in cell production. [ABSTRACT FROM AUTHOR]

Background: The major fleshy tissues of the apple fruit are spatially separable into cortex and pith. These tissues display differential growth during development. Key features of such differential growth, and sink metabolic programs supporting it have not been investigated previously. We hypothesized that differential growth between these fruit tissues is supported by differential sink metabolic programs, particularly during early development. Growth, metabolite concentrations, and transcript abundance of metabolism-related genes were measured to determine characteristics of differential growth and their underlying metabolic programs. Results: The cortex displayed > 5-fold higher growth than the pith during early fruit development, indicating that differential growth was established during this period. Further, when resource availability was increased through sink-removal, cortex growth was preferentially enhanced. Greatest diversity in metabolic programs between these tissues was evident during early fruit development. Higher cortex growth during early development was facilitated by increased catabolism of imported carbon (C) resources, sorbitol and sucrose, and the nitrogen (N) resource, asparagine. It was also associated with enhanced primary C metabolism, and C storage as malate and quinate. The pith metabolic program during this period involved limited allocation of C and N to growth, but greater allocation to storage, and enhanced sucrose-sucrose cycling. Conclusions: Together, these data indicate that the fruit cortex tissue displays a resource intensive metabolic program during early fruit development. This provides the C backbones, proteins, energy and osmolytes to support its higher growth. [ABSTRACT FROM AUTHOR]

Electrode manufacturing requires multiple process steps, e.g., dispersing and coating. In‐between these steps, intermediate products have to be transferred, stored, and handled. Especially for the development of new active materials or electrode formulations, the variety of parameters that need to be screened is enormous. In addition, these materials are initially tested in small batches, and it is not always possible to upscale the used processes. To evaluate the performance of different materials or differently processed materials, test cells are assembled. This usually requires manual work procedures, which are inherently sensitive to variations and untraceable errors. If the stochastic flaws are large enough, the effects of process variations are covered by these. It is therefore important to increase reproducibility in all process steps. Herein, new concepts for electrode production and automated sample preparation for highly reproducible production and more effective electrode development and screening of parameters are presented. A combined grinding and dispersion process for the production of silicon‐based anodes and an automated assembly system for efficient testing is presented. The processes are supported by methods of data mining to collect process data, ensure high reproducibility, and support research on new active materials. [ABSTRACT FROM AUTHOR]

Many data are available on expansion protocols for mesenchymal stromal cells (MSCs) for both experimental settings and manufacturing for clinical trials. However, there is a lack of information on translation of established protocols for Good Manufacturing Practice (GMP) from validation to manufacturing for clinical application. We present the validation and translation of a standardized pre-clinical protocol for isolation and expansion of MSCs for a clinical trial for reconstitution of alveolar bone. Key parameters of 22 large-scale expansions of MSCs from bone marrow (BM) for validation were compared with 11 expansions manufactured for the clinical trial "Jaw bone reconstruction using a combination of autologous mesenchymal stromal cells and biomaterial prior to dental implant placement (MAXILLO1)" aimed at reconstruction of alveolar bone. Despite variations of the starting material, the robust protocol led to stable performance characteristics of expanded MSCs. Manufacturing of the autologous advanced therapy medicinal product MAXILLO-1-MSC was possible, requiring 21 days for each product. Transport of BM aspirates and MSCs within 24 h was guaranteed. MSCs fulfilled quality criteria requested by the national competent authority. In one case, the delivered MSCs developed a mosaic in chromosomal finding, showing no abnormality in differentiation capacity, growth behavior or surface marker expression during long-term culture. The proportion of cells with the mosaic decreased in long-term culture and cells stopped growth after 38.4 population doublings. Clinical use of freshly prepared MSCs, manufactured according to a standardized and validated protocol, is feasible for bone regeneration, even if there was a long local distance between manufacturing center and clinical site. Several parameters, such as colony forming units fibroblasts (CFU-F), percentage of CD34+ cells, cell count of mononuclear cells (MNCs) and white blood cells (WBCs), of the BM may serve as a predictive tool for the yield of MSCs and may help to avoid unnecessary costs for MSC manufacturing due to insufficient cell expansion rates. [ABSTRACT FROM AUTHOR]

Purpose: In this paper, we have proposed a new model for designing a Cellular Manufacturing System (CMS) for minimizing the costs regarding a limited number of cells to be formed by assigning workforce. Design/methodology/approach: Pursuing mathematical approach and because the problem is NP-Hard, two meta-heuristic methods of Simulated Annealing (SA) and Particle Swarm Optimization (PSO) algorithms have been used. A small randomly generated test problem with real-world dimensions has been solved using simulated annealing and particle swarm algorithms. Findings: The quality of the two algorithms has been compared. The results showed that PSO algorithm provides more satisfactory solutions than SA algorithm in designing a CMS under uncertainty demands regarding the workforce allocation. Originality/value: In the most of the previous research, cell production has been considered under certainty production or demand conditions, while in practice production and demand are in a dynamic situations and in the real settings, cell production problems require variables and active constraints for each different time periods to achieve better design, so modeling such a problem in dynamic structure leads to more complexity while getting more applicability. The contribution of this paper is providing a new model by considering dynamic production times and uncertainty demands in designing cells. [ABSTRACT FROM AUTHOR]

The adoptive transfer of chimeric antigen receptor (CAR)-modified T cells is attracting growing interest for the treatment of malignant diseases. Early trials with anti-CD19 CAR T cells have achieved spectacular remissions in B-cell leukemia and lymphoma, so far refractory, very recently resulting in the Food and Drug Administration approval of CD19 CAR T cells for therapy. With further applications and increasing numbers of patients, the reproducible manufacture of high-quality clinical-grade CAR T cells is becoming an ever greater challenge. New processing techniques, quality-control mechanisms, and logistic developments are required to meet both medical needs and regulatory restrictions. This paper summarizes the state-of-the-art in manufacturing CAR T cells and the current challenges that need to be overcome to implement this type of cell therapy in the treatment of a variety of malignant diseases and in a greater number of patients. [ABSTRACT FROM AUTHOR]

Sandy surface sediments of tidal flats exhibit high microbial activity due to the fast and deep-reaching transport of oxygen and nutrients by porewater advection. On the other hand during low tide, limited transport results in nutrient and oxygen depletion concomitant to the accumulation of microbial metabolites. This study represents the first attempt to use flow-through reactors to investigate virus production, virus transport and the impact of tides and season in permeable sediments. The reactors were filled with intertidal sands of two sites (North beach site and backbarrier sand flat of Spiekeroog island in the German Wadden Sea) to best simulate advective porewater transport through the sediments. Virus and cell release along with oxygen consumption were measured in the effluents of reactors during continuous flow of water through the sediments as well as in tidal simulation experiments where alternating cycles with and without water flow (each for 6 h) were operated. The results showed net rates of virus production (0.3–13.2 × 106 viruses cm−3 h−1) and prokaryotic cell production (0.3–10.0 × 105 cells cm−3 h−1) as well as oxygen consumption rates (56–737 μmol l−1 h−1) to be linearly correlated reflecting differences in activity, season and location of the sediments. Calculations show that total virus turnover was fast with 2 to 4 days, whereas virus-mediated cell turnover was calculated to range between 5–13 or 33–91 days depending on the assumed burst sizes (number of viruses released upon cell lysis) of 14 or 100 viruses, respectively. During the experiments, the homogenized sediments in the reactors became vertically structured with decreasing microbial activities and increasing impact of viruses on prokaryotic mortality with depth. Tidal simulation clearly showed a strong accumulation of viruses and cells in the top sections of the reactors when the flow was halted indicating a consistently high virus production during low tide. In conclusion, cell lysis products due to virus production may fuel microbial communities in the absence of advection-driven nutrient input, but are eventually washed off the surface sediment during high tide and being transported into deeper sediment layers or into the water column together with the produced viruses.

Sandy surface sediments of tidal flats exhibit high microbial activity due to the fast and deep-reaching transport of oxygen and nutrients by porewater advection. On the other hand during low tide, limited transport results in nutrient and oxygen depletion concomitant to the accumulation of microbial metabolites. This study represents the first attempt to use flow-through reactors to investigate virus production, virus transport and the impact of tides and season in permeable sediments. The reactors were filled with intertidal sands of two sites (North beach site and backbarrier sand flat of Spiekeroog island in the German Wadden Sea) to best simulate advective porewater transport through the sediments. Virus and cell release along with oxygen consumption were measured in the effluents of reactors during continuous flow of water through the sediments as well as in tidal simulation experiments where alternating cycles with and without water flow (each for 6 h) were operated. The results showed net rates of virus production (0.3-13.2 × 106 viruses cm-3 h-1) and prokaryotic cell production (0.3-10.0 × 105 cells cm-3 h-1) as well as oxygen consumption rates (56-737 µmol l-1 h-1) to be linearly correlated reflecting differences in activity, season and location of the sediments. Calculations show that total virus turnover was fast with 2 to 4 days, whereas virus-mediated cell turnover was calculated to range between 5-13 or 33-91 days depending on the assumed burst sizes (number of viruses released upon cell lysis) of 14 or 100 viruses, respectively. During the experiments, the homogenized sediments in the reactors became vertically structured with decreasing microbial activities and increasing impact of viruses on prokaryotic mortality with depth. Tidal simulation clearly showed a strong accumulation of viruses and cells in the top sections of the reactors when the flow was halted indicating a consistently high virus production during low tide. In conclusion, cell lysis products due to virus production may fuel microbial communities in the absence of advection-driven nutrient input, but are eventually washed off the surface sediment during high tide and being transported into deeper sediment layers or into the water column together with the produced viruses. [ABSTRACT FROM AUTHOR]

Hospital Montfort has doubled its size in the past years and this has had an enormous impact on the number of new orders received in pharmacy. Our department was having difficulty to cope with this increased workload and sending new medication to the nursing units in a timely fashion. The pharmacy team used a lean initiative to improve its turnaround time for a new order. Using some key principles, the pharmacy staff at Hospital Montfort were able to rapidly decrease its turnaround time for a new prescription by 47% from an average of 2:08h to 1:21h. The implication of staff in this project also helped decrease the overtime by 69%. It seems like this relatively innovative approach in Canada provides results and was useful in building a stronger team. [ABSTRACT FROM AUTHOR]

Boreal forest ecosystems are a crucial element in the global climate balance. In harsh environments functioning lateral meristems of trees are more regulated by the exogenous (included local climate) than endogenous factors. This information is encoded in the tree-ring structure which can be effectively decomposed by the process-based tree-ring growth simulations. Moreover, the process-based modeling can be used to describe non-linear processes linking climate variables with tree-ring formation. In this study, we applied the Vaganov-Shashkin model to simulate seasonal cell production and cell enlargement of Larix gmelinii Rupr. (Rupr.) growing in the permafrost zone of Central Siberia. We developed a procedure for calculating the radial cell diameter based on specific Gompertz function combined with the "instantaneous tracheidogram" approach to estimate effectively seasonal cell production and timing of cell enlargement under climate control. Simulated cell number and cell size matched well with direct xylogenesis observations. The developed procedure demonstrate strong relationships between seasonal simulated growth rate and measured tracheid radial size (the average correlation is 0.64, p < 0.01). A highly significant correlation (p < 0.01) between simulated and observed cell profiles was obtained for 71% of the growing seasons over the period 1950–2011. The strong exponential relationship (R 2 = 0.67) was obtained between the day of the year (DOY) when cambial cell transfers into enlargement zone and simulated time intervals of cell enlargement. Based on the strong exponential relationship it was possible to reproduce the basic pattern of the observed tracheidograms over 1950–2011 with a systematic overestimation of final cell sizes at the beginning of the growing season, which will be possible to eliminate by using more anatomical data (trees) and longer period. The proposed approach of simulating intra-annual cell dynamics (cell production) has a great potential for studying how climate affects tree-ring formation. [Display omitted] • VS-model is used to estimate cell production and enlargement of larch trees growing in permafrost zone. • The effective procedure for estimation the radial cell enlarging and its timing under climate control was developed. • Low growth rates at the beginning of the growing season correspond to a longer period of appearance of cambial cells in the enlargement zone. [ABSTRACT FROM AUTHOR]

The interaction between xylem phenology and climate assesses forest growth and productivity and carbon storage across biomes under changing environmental conditions. We tested the hypothesis that patterns of wood formation are maintained unaltered despite the temperature changes across cold ecosystems. Wood microcores were collected weekly or biweekly throughout the growing season for periods varying between 1 and 13 years during 1998-2014 and cut in transverse sections for assessing the onset and ending of the phases of xylem differentiation. The data set represented 1321 trees belonging to 10 conifer species from 39 sites in the Northern Hemisphere and covering an interval of mean annual temperature exceeding 14 K. The phenological events and mean annual temperature of the sites were related linearly, with spring and autumnal events being separated by constant intervals across the range of temperature analysed. At increasing temperature, first enlarging, wall-thickening and mature tracheids appeared earlier, and last enlarging and wall-thickening tracheids occurred later. Overall, the period of wood formation lengthened linearly with the mean annual temperature, from 83.7 days at −2 °C to 178.1 days at 12 °C, at a rate of 6.5 days °C−1. April-May temperatures produced the best models predicting the dates of wood formation. Our findings demonstrated the uniformity of the process of wood formation and the importance of the environmental conditions occurring at the time of growth resumption. Under warming scenarios, the period of wood formation might lengthen synchronously in the cold biomes of the Northern Hemisphere. [ABSTRACT FROM AUTHOR]

The demands placed on automation of cell production processes for IT products are becoming increasingly sophisticated. Dual-arm robots are drawing particular attention as a process solution because they offer flexibility and can work in a similar manner to a human operator. In this paper, we propose an automation system for cellular phone packing processes that uses two dual-arm robots. The applied robots are designed with specifications that meet the requirements of the cellular phone packing tasks. In addition, a robotic cell production system is proposed that applies a task allocation method to perform an efficient packing job for cellular phones. In particular, each task is assigned with the intention of reducing the takt time , which is the time taken to finish a single product in the production line and to avoid collisions between the two robots. Finally, we implement some of our results in a demonstration of a packing job that involves filling five unit boxes with seven kinds of accessories. [ABSTRACT FROM AUTHOR]

Purpose: This research, by using the SIMIO simulation platform, provides a quantitative and comparative analysis of how the efficiency of four different cell production modes is affected. It is hoped that the results will be of some help for garment factories to optimize their production lines. Design/methodology/approach: The SIMIO simulation platform was employed in the research and comparisons were made of the simulation test results about the four popular cellular manufacturing modes. Findings: The operation mode, number of operators, and number of buffer areas are key factors affecting the production line efficiency, and need to be reasonably set to achieve the highest efficiency. Originality/value: As most research literature so far is qualitative, this research provided a simulation-based quantitative analysis of the production efficiency under different cell production modes. [ABSTRACT FROM AUTHOR]

Neighboring trees growing under identical environmental conditions can exhibit different dynamics and periods of growth. Despite the recent advances in cambial biology, the exogenous and endogenous factors generating asynchronous xylem growths still remain undetermined. This study investigated timings and duration of xylem formation in maritime pine ( Pinus pinaster Ait.) from an even-aged plantation in Portugal growing under Mediterranean climate. Cambial phenology and stem diameter were monitored weekly, from March to December 2010, on two classes of trees divided according to the tree ring widths of the last 15 years, but similar age and size: fast- and slow-growing trees. We tested the hypothesis that differences in tree ring widths result from cell production which in turn affects timings of xylogenesis and that the bimodal growth pattern, typical of the Mediterranean, originates from a double reactivation of the cambium: in spring and autumn. Cambial activity started earlier and ended later in fast-growing trees, confirming that cell production is a key factor determining the duration of xylogenesis. Intra-annual variations in stem diameter recorded by band dendrometers revealed two peaks of increment occurring in spring and late summer. However, the number of cambial cells did not increase in late summer, which suggested that the second peak of increment was caused by stem rehydration, rather than by a reactivation of cell division. These results demonstrated that the variability in the timings of xylem phenology observed among trees of the same age and size and growing under similar environmental conditions was closely related to cell production and not to age or size per se. [ABSTRACT FROM AUTHOR]

Background and Aims Ongoing global warming has been implicated in shifting phenological patterns such as the timing and duration of the growing season across a wide variety of ecosystems. Linear models are routinely used to extrapolate these observed shifts in phenology into the future and to estimate changes in associated ecosystem properties such as net primary productivity. Yet, in nature, linear relationships may be special cases. Biological processes frequently follow more complex, non-linear patterns according to limiting factors that generate shifts and discontinuities, or contain thresholds beyond which responses change abruptly. This study investigates to what extent cambium phenology is associated with xylem growth and differentiation across conifer species of the northern hemisphere. Methods Xylem cell production is compared with the periods of cambial activity and cell differentiation assessed on a weekly time scale on histological sections of cambium and wood tissue collected from the stems of nine species in Canada and Europe over 1–9 years per site from 1998 to 2011. Key Results The dynamics of xylogenesis were surprisingly homogeneous among conifer species, although dispersions from the average were obviously observed. Within the range analysed, the relationships between the phenological timings were linear, with several slopes showing values close to or not statistically different from 1. The relationships between the phenological timings and cell production were distinctly non-linear, and involved an exponential pattern Conclusions The trees adjust their phenological timings according to linear patterns. Thus, shifts of one phenological phase are associated with synchronous and comparable shifts of the successive phases. However, small increases in the duration of xylogenesis could correspond to a substantial increase in cell production. The findings suggest that the length of the growing season and the resulting amount of growth could respond differently to changes in environmental conditions. [ABSTRACT FROM AUTHOR]

Lactic acid and cell production from whey permeate by Lactobacillus rhamnosus with different nutrient supplements were investigated in this study. Yeast extract was identified as the most effective nutrient affecting lactic acid production. Increase in inoculum size from 0.05% to 1% ( v/ v) resulted in a substantial increase in lactic acid productivity from 0.66 to 0.83 g L h ( P < 0.001). The optimal temperature for lactic acid production was 37 °C, while the highest cell production was obtained at 42 °C. When whey permeate and yeast extract concentrations were 6.8% ( w/ v) and 3 g L, respectively, lactic acid productivity reached 0.85 g L h after 48-h cultivation, which is 3.40 times of those without nutrient supplements. [ABSTRACT FROM AUTHOR]

Although habitually considered as a whole, xylogenesis is a complex process of division and maturation of a pool of cells where the relationship between the phenological phases generating such a growth pattern remains essentially unknown. This study investigated the causal relationships in cambium phenology of black spruce [Picea mariana (Mill.) BSP] monitored for 8 years on four sites of the boreal forest of Quebec, Canada. The dependency links connecting the timing of xylem cell differentiation and cell production were defined and the resulting causal model was analysed with d-sep tests and generalized mixed models with repeated measurements, and tested with Fisher’s C statistics to determine whether and how causality propagates through the measured variables. The higher correlations were observed between the dates of emergence of the first developing cells and between the ending of the differentiation phases, while the number of cells was significantly correlated with all phenological phases. The model with eight dependency links was statistically valid for explaining the causes and correlations between the dynamics of cambium phenology. Causal modelling suggested that the phenological phases involved in xylogenesis are closely interconnected by complex relationships of cause and effect, with the onset of cell differentiation being the main factor directly or indirectly triggering all successive phases of xylem maturation. [ABSTRACT FROM PUBLISHER]

Delays in the start of the growing season reduce the period available for growth and the amount of xylem production. However, a higher number of developing tracheids could prolong cell differentiation and, consequently, lengthen the growing season. The relationship between the amount and duration of cell production in the xylem remains an unresolved issue. The aim of this study was to resolve the chicken-or-egg causality dilemma about duration of growth and cell production through simple- and double-cause models. This was achieved by (1) analysing the intra-annual growth dynamics of the xylem in Picea mariana (Mill.) BSP during 2006–2009 in two contrasting sites of the boreal forest of Quebec, Canada, and (2) extracting the dates of onset and ending of xylem formation and the number of radial cells along the tree ring. A higher number of cells was linked to an earlier onset ( r = 0.74) and later ending ( r = 0.61) of cell differentiation. The absence of a relationship between the residuals of the onset and ending of xylogenesis ( rp = −0.06) indicated that cell production influenced the correlation between the two phenophases of the xylem. These results demonstrated that a higher number of cells produced delay the ending of xylem maturation, so extending the duration of wood formation. [ABSTRACT FROM AUTHOR]

Since a human operator enables a cell production system to flexibly meet the diversified production and quantity requirements, monitoring and optimizing the operator's assembly motion can improve the assembly efficiency. This paper describes an operator monitoring system to monitor an operator's assembly motion in real-time. To overcome the changing light conditions in actual factories, a special color filter method was used to detect the color marks attached to the operator's body. To determine whether the detected data are correct, a kinematic human body simulator was built to regenerate an operator's motions in real-time. According to the experimental results, the operator monitoring system meets the measurement accuracy requirement. Furthermore, this system can easily be adjusted to satisfy the changing light conditions. When the operator executes the assembly task using a bad motion, based on the assembly motion model database, our monitoring system can provide information to guide the operator and optimize his/her assembly motion. Furthermore, this system can also recognize the operator's states. Due to the characteristics of this method, it can be used to conveniently optimize the operator's assembly process in the actual manufacturing fields. [ABSTRACT FROM AUTHOR]

• Five families of organ growth regulating genes were identified and evaluated in apple in relation to fruit growth. • Two MdARGOS genes were identified as associated with fine-tuning cell production during apple fruit growth. • Two MdCNR genes were negatively associated with regulation of cell production during early fruit development. • A MdGRF-MdGIF module was associated with spatio-temporal regulation of cell production during early fruit development. Fruit size is a commercially valuable trait that is determined by the extent of fruit growth. In apple, (Malus × domestica Borkh.), early fruit growth is facilitated by intensive cell production, but mechanisms regulating these processes are not well understood. In this study, members of five gene families involved in growth regulation were identified and evaluated in apple: AUXIN-REGULATED GENE INVOLVED IN ORGAN SIZE (ARGOS); CELL NUMBER REGULATOR (CNR); GROWTH REGULATING FACTOR (GRF); GRF INTERACTING FACTOR (GIF); and KLUH. MdARGOS1 and MdARGOS2 transcript abundance was higher during early fruit growth but was also enhanced by up to 18-fold under conditions of reduced cell production during flower and fruit development, indicating that their gene products play complex roles in regulating growth mechanisms during early development. MdCNR20a and MdCNR20b transcript abundance decreased during early fruit growth and increased during the transition from cell production to cell expansion. MdCNR20a transcript abundance was negatively correlated with the relative cell production rate (RCPR; ρ = - 0.82; P = 0.002), was enhanced in response to shading, and reduced in response to lower fruit load, indicating that it's gene product negatively regulates cell production and early fruit growth. Transcript abundance of MdGRF11 and MdGRF16 was greater during cell production-mediated fruit growth, and higher in the cortex than in the pith. MdGIF3 transcript abundance was greater during early fruit development, higher in the cortex, enhanced under reduced fruit load conditions, and strongly correlated with RCPR (ρ = 0.93; P < 0.0001). Further, it was correlated with that of MdGRF11 and MdGRF16 (ρ = 0.87 and 0.89, respectively; P < 0.0001), indicating that the GRF11/16-GIF3 module is co-regulated and involved in spatio-temporal regulation of cell production and fruit growth. Transcript abundance of MdKLUH1 was low and not consistent with a role in regulating cell production and fruit growth. Together, this study demonstrates coordinated control of cell production during early fruit growth by multiple regulatory components, identifies candidates for functional characterization, and provides potential tools for enhancing growth of the apple fruit. [ABSTRACT FROM AUTHOR]