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This study aimed to analyze the protein complex and the content of selected microelements in the grain of seven Triticum dicoccum (Schrank ex. Schübl) genotypes. Discontinuous fractionation of the grain protein complex shows that gliadins (60.69%) were the most predominant fraction. Together with glutenins, they represent storage proteins with a share of 82.30%. Individual genotypes differed significantly in the amount of cytoplasmic proteins represented by albumins and globulins. SDS-PAGE results show that the majority of proteins were fractions with a relative molecular weight of 33 kDa – 96 kDa. Using ELISA with G12 monoclonal antibody high content of celiac active proteins was recorded, specifically 58.63 – 83.45 mg/kg of grain. Triticum dicoccum is therefore not suitable for celiacs. The ICP-OES method was used to determine the content of microelements. Of the nutritionally significant microelements, Triticum dicoccum contained important K, Mg, Ca, Zn, Fe and Mn concentrations.