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2 results on '"caldocellum-saccharolyticum"'

1. Hydrogenomics of the extremely thermophilic bacterium Caldicellulosiruptor saccharolyticus

Author

Heleen P. Goorissen, Marcel R. A. Verhaart, Ed W. J. van Niel, Willem M. de Vos, Emmanuel F. Mongodin, Karen E. Nelson, Harmen J.G. van de Werken, Servé W. M. Kengen, Donald E. Ward, Alfons J. M. Stams, Robert M. Kelly, John van der Oost, Jason D. Nichols, Derrick L. Lewis, Amy L. VanFossen, Karin Willquist, and Immunology

Subjects
DNA, Bacterial, Glucuronate, Caldicellulosiruptor, Molecular Sequence Data, Catabolite repression, Xylose, dna, 7. Clean energy, Applied Microbiology and Biotechnology, Microbiology, thermotoga-maritima, 03 medical and health sciences, chemistry.chemical_compound, Bacterial Proteins, Microbiologie, expression, Hemicellulose, Sugar transporter, gene, bacillus-subtilis, Caldicellulosiruptor bescii, caldocellum-saccharolyticum, 030304 developmental biology, VLAG, 0303 health sciences, WIMEK, Ecology, biology, 030306 microbiology, Gene Expression Profiling, Sequence Analysis, DNA, anaerobic-bacteria, sequence, Physiology and Biotechnology, biology.organism_classification, Enzymes, chemistry, Biochemistry, gram-positive bacteria, Carbohydrate Metabolism, identification, Caldicellulosiruptor saccharolyticus, Genome, Bacterial, Metabolic Networks and Pathways, Food Science, Biotechnology
Abstract

Caldicellulosiruptor saccharolyticus is an extremely thermophilic, gram-positive anaerobe which ferments cellulose-, hemicellulose- and pectin-containing biomass to acetate, CO 2 , and hydrogen. Its broad substrate range, high hydrogen-producing capacity, and ability to coutilize glucose and xylose make this bacterium an attractive candidate for microbial bioenergy production. Here, the complete genome sequence of C. saccharolyticus , consisting of a 2,970,275-bp circular chromosome encoding 2,679 predicted proteins, is described. Analysis of the genome revealed that C. saccharolyticus has an extensive polysaccharide-hydrolyzing capacity for cellulose, hemicellulose, pectin, and starch, coupled to a large number of ABC transporters for monomeric and oligomeric sugar uptake. The components of the Embden-Meyerhof and nonoxidative pentose phosphate pathways are all present; however, there is no evidence that an Entner-Doudoroff pathway is present. Catabolic pathways for a range of sugars, including rhamnose, fucose, arabinose, glucuronate, fructose, and galactose, were identified. These pathways lead to the production of NADH and reduced ferredoxin. NADH and reduced ferredoxin are subsequently used by two distinct hydrogenases to generate hydrogen. Whole-genome transcriptome analysis revealed that there is significant upregulation of the glycolytic pathway and an ABC-type sugar transporter during growth on glucose and xylose, indicating that C. saccharolyticus coferments these sugars unimpeded by glucose-based catabolite repression. The capacity to simultaneously process and utilize a range of carbohydrates associated with biomass feedstocks is a highly desirable feature of this lignocellulose-utilizing, biofuel-producing bacterium.

Published
2008

2. Hydrogenomics of the extremely thermophilic bacterium Caldicellulosiruptor saccharolyticus

Author

van de Werken, H.J.G., Verhaart, M.R.A., Vanfossen, A.L., Willquist, K., Lewis, D.L., Nichols, J.D., Goorissen, H.P., Mongodin, E.F., Nelson, K.E., van Niel, E.W.J., Stams, A.J.M., Ward, D.E., de Vos, W.M., van der Oost, J., Kelly, R.M., Kengen, S.W.M., van de Werken, H.J.G., Verhaart, M.R.A., Vanfossen, A.L., Willquist, K., Lewis, D.L., Nichols, J.D., Goorissen, H.P., Mongodin, E.F., Nelson, K.E., van Niel, E.W.J., Stams, A.J.M., Ward, D.E., de Vos, W.M., van der Oost, J., Kelly, R.M., and Kengen, S.W.M.

Abstract

Caldicellulosiruptor saccharolyticus is an extremely thermophilic, gram-positive anaerobe which ferments cellulose-, hemicellulose- and pectin-containing biomass to acetate, CO(2), and hydrogen. Its broad substrate range, high hydrogen-producing capacity, and ability to coutilize glucose and xylose make this bacterium an attractive candidate for microbial bioenergy production. Here, the complete genome sequence of C. saccharolyticus, consisting of a 2,970,275-bp circular chromosome encoding 2,679 predicted proteins, is described. Analysis of the genome revealed that C. saccharolyticus has an extensive polysaccharide-hydrolyzing capacity for cellulose, hemicellulose, pectin, and starch, coupled to a large number of ABC transporters for monomeric and oligomeric sugar uptake. The components of the Embden-Meyerhof and nonoxidative pentose phosphate pathways are all present; however, there is no evidence that an Entner-Doudoroff pathway is present. Catabolic pathways for a range of sugars, including rhamnose, fucose, arabinose, glucuronate, fructose, and galactose, were identified. These pathways lead to the production of NADH and reduced ferredoxin. NADH and reduced ferredoxin are subsequently used by two distinct hydrogenases to generate hydrogen. Whole-genome transcriptome analysis revealed that there is significant upregulation of the glycolytic pathway and an ABC-type sugar transporter during growth on glucose and xylose, indicating that C. saccharolyticus coferments these sugars unimpeded by glucose-based catabolite repression. The capacity to simultaneously process and utilize a range of carbohydrates associated with biomass feedstocks is a highly desirable feature of this lignocellulose-utilizing, biofuel-producing bacterium

Published
2008

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