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In a three-phase symmetric power system, we propose a transformation that converts the original current to a current with minimal losses while preserving the standard constraints. The selected transformation is realized in suitable geometric algebra and is not time-dependent. The proposed transformation uses the group symmetry of conformal geometric algebra, mainly rotations and tations. [ABSTRACT FROM AUTHOR]

Simple Summary: Previous studies have shown that mitochondrial dysfunction, cytoplasmic abnormalities, and abnormal embryonic development are linked to excessive intracellular production of reactive oxygen species (ROS). Chlorogenic acid (CGA), a polyphenol widely found in plants, has been shown to have strong antioxidant properties by forming hydrogen radicals to eliminate the activity of free radicals such as hydroxyl radicals and superoxide anions. Thus, this study investigated the effects of CGA on oxidative stress and embryonic development of sheep oocytes by adding different CGA concentrations during in vitro maturation and vitrification freezing. The results showed that CGA effectively alleviates the oxidative stress damage of sheep oocytes, increases their maturation, cleavage, and blastocyst rates after solitary female activation, and promotes embryo development. Chlorogenic acid (CGA) has strong antioxidant properties. In order to improve the low maturation rate and poor vitrification freezing effect of sheep oocytes caused by oxidative stress. In this study, oocytes from 200 2–3-year-old Kazakh sheep were collected, and different concentrations of CGA were added to the maturation medium and vitrification freezing solution to study the effects of CGA on the maturation rate, cleavage rate, blastocyst rate, reactive oxygen species (ROS) and glutathione (GSH) levels, mitochondrial membrane potential, and the expression levels of oxidation and apoptosis-related genes in sheep oocytes. The results showed that adding 40 μmol/L CGA to the oocyte in vitro maturation solution significantly increased the maturation rate of oocytes, adding 50 μmol/L CGA to the vitrification cryopreservative solution significantly increased the cleavage and blastocyst rates of mature oocytes activated by parthenogenetic activation after freezing. During in vitro maturation and vitrification freezing in sheep oocytes, CGA significantly reduced the level of ROS and the expression of apoptosis-related genes (Caspase-3 and Bax/Bcl-2), and significantly increased the level of glutathione (GSH), mitochondrial membrane potential, and the expression of antioxidant and anti-apoptosis-related genes (SOD-2 and GPX-3). In addition, CGA significantly increased the expression of the anti-apoptotic gene (AKT) and anti-stress gene (FOXO) during vitrification freezing of sheep oocytes. In conclusion, 40 μmol/L CGA improves the maturation rate of sheep oocytes, and 50 μmol/L CGA improves the quality of parthenogenetic activation embryos after vitrification freezing of mature oocytes in sheep. These results provide a basis for the production of sheep in vitro embryos and the establishment of a germplasm resource bank. [ABSTRACT FROM AUTHOR]

Mammalian genomes contain thousands of genes for long noncoding RNA (lncRNAs), some of which have been shown to affect protein coding gene expression through diverse mechanisms. The lncRNA transcripts are longer than 200 nucleotides and are often capped, spliced, and polyadenylated, but not translated into protein. Nuclear lncRNAs can modify chromatin structure and transcription in trans or cis by interacting with the DNA, forming R-loops, and recruiting regulatory proteins. Not much is known about the role of lncRNA in pituitary gland differentiation and function. We mined transcriptome data from mouse pituitary glands collected at embryonic days 12.5 and 14.5 and identified over 200 different lncRNA transcripts. To develop a research resource for the study of lncRNA, we used pituitary cre transgenes to tag pituitary cell types in adult mice with fluorescent markers, and enriched for thyrotropes, gonadotropes, and somatotropes using fluorescence-activated cell sorting. We determined the transcriptome of each cell population using RNA sequencing and mined the data for lncRNA. We detected hundreds of lncRNAs in adult pituitary cells; a few were located immediately nearby genes that encode pituitary hormones or lineage-specific transcription factors. The location of these lncRNAs suggests the possibility of a cis -acting regulatory role in pituitary development or function, and we observe coordinated expression of 2 of them with their putative target genes in transgenic mice. This research resource sets the foundation for examining the actions of lncRNAs on their putative target genes and determining whether they have roles during development and in response to physiological demand. [ABSTRACT FROM AUTHOR]

Breast neuroendocrine carcinoma (NECB) is a rare type of breast tumor. Large-cell neuroendocrine carcinomas of the breast (LCNECB) are a special and rare histological subtype of NECB. Here, we present a case of a 59-year-old woman who was diagnosed with an LCNECB. A mass in the upper outer quadrant of the right breast was revealed via imaging. A histological examination showed the tumor cells were composed of clusters of large cells with obvious atypia that were polygonal or irregularly shaped. The patient underwent a right-breast-conserving radical surgery and sentinel lymph node biopsy (SLB). A histopathological examination revealed that the tumor of the right breast was 2.5 × 2 cm in size with vascular invasion, and the sentinel lymph node was negative. The immunohistochemical results showed that the tumor cells were diffuse and positive for chromogranin A (CgA), synaptophysin (Syn), and INSM1. The patient successfully completed chemotherapy and radiotherapy and is currently undergoing endocrine therapy. [ABSTRACT FROM AUTHOR]

Using the classification of Clifford algebras and Bott periodicity, we show how higher geometric algebras can be realized as matrices over classical low dimensional geometric algebras. This matrix representation allows us to use standard geometric algebra software packages more easily. As an example, we express the geometric algebra for conics (GAC) as a matrix over the Compass ruler algebra (CRA). [ABSTRACT FROM AUTHOR]

Strawberries are a commonly used agricultural product in the food industry. In the traditional production model, labor costs are high, and extensive picking techniques can result in food safety issues, like poor taste and fruit rot. In response to the existing challenges of low detection accuracy and slow detection speed in the assessment of strawberry fruit maturity in orchards, a CR-YOLOv9 multi-stage method for strawberry fruit maturity detection was introduced. The composite thinning network, CRNet, is utilized for target fusion, employing multi-branch blocks to enhance images by restoring high-frequency details. To address the issue of low computational efficiency in the multi-head self-attention (MHSA) model due to redundant attention heads, the design concept of CGA is introduced. This concept aligns input feature grouping with the number of attention heads, offering the distinct segmentation of complete features for each attention head, thereby reducing computational redundancy. A hybrid operator, ACmix, is proposed to enhance the efficiency of image classification and target detection. Additionally, the Inner-IoU concept, in conjunction with Shape-IoU, is introduced to replace the original loss function, thereby enhancing the accuracy of detecting small targets in complex scenes. The experimental results demonstrate that CR-YOLOv9 achieves a precision rate of 97.52%, a recall rate of 95.34%, and an mAP@50 of 97.95%. These values are notably higher than those of YOLOv9 by 4.2%, 5.07%, and 3.34%. Furthermore, the detection speed of CR-YOLOv9 is 84, making it suitable for the real-time detection of strawberry ripeness in orchards. The results demonstrate that the CR-YOLOv9 algorithm discussed in this study exhibits high detection accuracy and rapid detection speed. This enables more efficient and automated strawberry picking, meeting the public's requirements for food safety. [ABSTRACT FROM AUTHOR]

Introduction: Diabetes Mellitus (DM) is a chronic disease with various complications, one of which is Diabetic Encephalopathy (ED). Hyperglycemia in DM will result in neuroinflammation through the proinflammatory M1 pathway. Some proinflammatory agents will increase with the activation of this pathway, such as IL-6. The neuroglia is then activated and expresses CD86 on the cell membrane. Chlorogenic acid (CGA) is a polyphenol compound in coffee that has an anti-inflammatory effect.The purpose of this study is to clarify the effect of CGA on the activation of M1 pathway microglia by looking at the expression of IL-6 and CD86 mRNA on the frontal lobe of mice with a DM model. Methods: 24 male rats were randomly divided into six groups, namely: control, 1.5-month DM and 2-month DM, and the group with CGA administration with three different doses (CGA1, CGA2, CGA3). Frontal lobe tissue was taken for analysis of IL-6 and CD86 mRNA expression using RT-PCR. Results: Frontal lobe IL-6 mRNA expression differed significantly between the control group with DM1.5 (p=0.010); control with DM2 (p=0.001); DM2 group with CGA2 (p=0.028). Frontal lobe CD86 mRNA expression differed significantly between the control group and all DM groups, either with CGA or without CGA (p<0.05). The DM2 group differed significantly from the CGA2 group (p=0.000) and the CGA3 group (p=0.000). Conclusion: The expression of mRNA markers of proinflammatory vessels M1 (IL-6 and CD86) in frontal lobe neuroinflammation due to DM was lower after administration of CGA at a dose of 25 mg/KgBB. [ABSTRACT FROM AUTHOR]

Malnutrition is a significant concern affecting the elderly, necessitating a complex assessment. This study aims to deepen the understanding of factors associated with the assessment of malnutrition in the elderly by comparing single- and multi-parameter approaches. In this cross-sectional study, 154 individuals underwent a comprehensive geriatric assessment (CGA). Malnutrition risk was determined using the mini nutritional assessment (MNA). Additional factors assessed included sarcopenia, polypharmacy, depression, appetite, handgrip strength, and gait speed. Phase angle (PA) and body composition were measured using bioelectrical impedance analysis (BIA). The MNA identified a malnutrition risk in 36.8% of individuals. The geriatric depression scale (GDS) and PA demonstrated moderate effectiveness in assessing malnutrition risk, with AUC values of 0.69 (95% CI: 0.60–0.78) and 0.62 (95% CI: 0.54–0.72), respectively. A logistic regression model incorporating handgrip strength, skeletal muscle mass, sarcopenia, osteoporosis, depression, specific antidepressant use, mobility, appetite, and smoking achieved superior performance in predicting malnutrition risk, with an AUC of 0.84 (95% CI: 0.77–0.91). In conclusion, this study demonstrates that integrating multiple parameters into a composite model provides a more accurate and comprehensive assessment of malnutrition risk in elderly adults. [ABSTRACT FROM AUTHOR]

Chlorogenic acid (CGA) exhibits promising anti-inflammatory properties, making it a potential therapeutic agent for inflammatory conditions and allergic rhinitis (AR). This study aimed to evaluate the therapeutic effects of CGA on inflammation in RAW264.7 macrophage cells and on AR in mice. RAW264.7 cells were treated with lipopolysaccharide (LPS) to induce inflammation and cultured with varying concentrations of CGA, a Tlr4-silenced gene (shTlr4) transfection, and the MAPK/NF-κB pathway activator diprovocim. Cell viability was assessed using the CCK8 assay, while levels of nitric oxide (NO), TNF-α, and IL-6 were measured by Griess colorimetry, immunofluorescence, and ELISA. Expression and phosphorylation levels of the MAPK/NF-κB pathway were evaluated using qPCR and western blotting. Additionally, ovalbumin (OVA)-induced AR mice received different doses of CGA, and Toll-like receptor-4 (Tlr4) overexpression was induced. In vitro, CGA treatment significantly reduced LPS-induced cell activity, NO, TNF-α, and IL-6 secretion, and downregulated Tlr4, p-p38, p-p65, and p-IκB expression. Tlr4 inhibition suppressed cell activity and inflammation by blocking MAPK/NF-κB pathway activation. Conversely, Tlr4 overexpression counteracted the effects of CGA, increasing cell activity and inflammatory factor concentration. In OVA-induced AR mice, CGA effectively alleviated allergic symptoms, reduced inflammatory factor secretion, and inhibited TLR4/MAPK/NF-κB pathway activity. These findings suggest CGA’s potential as an anti-inflammatory agent in RAW264.7 cells and AR models through modulation of the TLR4/MAPK/NF-κB pathway.

Background There is significant evidence on the benefits of comprehensive assessment in older adults. But this evidence is primarily from western countries and in secondary care settings. National policies in India recognize this need and envision community-based screening and facility-based assessment programs integrated into the care pathways for the elderly. However, this is yet to translate into specific interventions, primarily due to lack of complex interventions necessary and evidence of their effectiveness. This study aims to design and pilot an integrated (Community + Facility) Elderly Health Status Assessment and Screening (EHSAS) intervention to improve health outcomes of older adults and assess its feasibility for implementation in Indian rural settings. Methods We propose a hybrid design where we will build the complex intervention, develop and validate the tools needed, pilot it using an exploratory cluster randomized trial and evaluate its implementatbility using the Exploration-Preparation-Implementation-Sustainment (EPIS) framework. Conclusions This study will fill critical gaps in evidence regarding the effectiveness of geriatric screening and assessment in community and primary care settings in low-middle income countries and provide validated tools and implementation models for adoption into national programs. Registration CTRI/2023/07/055661

Abstract Background The Comprehensive Geriatric Assessment (CGA) records geriatric syndromes in a standardized manner, allowing individualized treatment tailored to the patient’s needs and resources. Its use has shown a beneficial effect on the functional outcome and survival of geriatric patients. A recently published German S1 guideline for level 2 CGA provides recommendations for the use of a broad variety of different assessment instruments for each geriatric syndrome. However, the actual use of assessment instruments in routine geriatric clinical practice and its consistency with the guideline and the current state of literature has not been investigated to date. Methods An online survey was developed by an expert group of geriatricians and sent to all licenced geriatricians (n = 569) within Germany. The survey included the following geriatric syndromes: motor function and self-help capability, cognition, depression, pain, dysphagia and nutrition, social status and comorbidity, pressure ulcers, language and speech, delirium, and frailty. Respondents were asked to report which geriatric assessment instruments are used to assess the respective syndromes. Results A total of 122 clinicians participated in the survey (response rate: 21%); after data cleaning, 76 data sets remained for analysis. All participants regularly used assessment instruments in the following categories: motor function, self-help capability, cognition, depression, and pain. The most frequently used instruments in these categories were the Timed Up and Go (TUG), the Barthel Index (BI), the Mini Mental State Examination (MMSE), the Geriatric Depression Scale (GDS), and the Visual Analogue Scale (VAS). Limited or heterogenous assessments are used in the following categories: delirium, frailty and social status. Conclusions Our results show that the assessment of motor function, self-help capability, cognition, depression, pain, and dysphagia and nutrition is consistent with the recommendations of the S1 guideline for level 2 CGA. Instruments recommended for more frequent use include the Short Physical Performance Battery (SPPB), the Montreal Cognitive Assessment (MoCA), and the WHO-5 (depression). There is a particular need for standardized assessment of delirium, frailty and social status. The harmonization of assessment instruments throughout geriatric departments shall enable more effective treatment and prevention of age-related diseases and syndromes.

Chlorogenic acid (CGA) has strong antioxidant properties. In order to improve the low maturation rate and poor vitrification freezing effect of sheep oocytes caused by oxidative stress. In this study, oocytes from 200 2–3-year-old Kazakh sheep were collected, and different concentrations of CGA were added to the maturation medium and vitrification freezing solution to study the effects of CGA on the maturation rate, cleavage rate, blastocyst rate, reactive oxygen species (ROS) and glutathione (GSH) levels, mitochondrial membrane potential, and the expression levels of oxidation and apoptosis-related genes in sheep oocytes. The results showed that adding 40 μmol/L CGA to the oocyte in vitro maturation solution significantly increased the maturation rate of oocytes, adding 50 μmol/L CGA to the vitrification cryopreservative solution significantly increased the cleavage and blastocyst rates of mature oocytes activated by parthenogenetic activation after freezing. During in vitro maturation and vitrification freezing in sheep oocytes, CGA significantly reduced the level of ROS and the expression of apoptosis-related genes (Caspase-3 and Bax/Bcl-2), and significantly increased the level of glutathione (GSH), mitochondrial membrane potential, and the expression of antioxidant and anti-apoptosis-related genes (SOD-2 and GPX-3). In addition, CGA significantly increased the expression of the anti-apoptotic gene (AKT) and anti-stress gene (FOXO) during vitrification freezing of sheep oocytes. In conclusion, 40 μmol/L CGA improves the maturation rate of sheep oocytes, and 50 μmol/L CGA improves the quality of parthenogenetic activation embryos after vitrification freezing of mature oocytes in sheep. These results provide a basis for the production of sheep in vitro embryos and the establishment of a germplasm resource bank.

Chemotherapeutic drugs are indispensable in cancer treatment, but their effectiveness is often lessened because of non-selective toxicity to healthy tissues, which triggers inflammatory pathways that are harmful to vital organs. In addition, tumors' resistance to drugs causes failures in treatment. Chlorogenic acid (5-caffeoylquinic acid, CGA), found in plants and vegetables, is promising in anticancer mechanisms. In vitro and animal studies have indicated that CGA can overcome resistance to conventional chemotherapeutics and alleviate chemotherapy-induced toxicity by scavenging free radicals effectively. This review is a summary of current information about CGA, including its natural sources, biosynthesis, metabolism, toxicology, role in combatting chemoresistance, and protective effects against chemotherapy-induced toxicity. It also emphasizes the potential of CGA as a pharmacological adjuvant in cancer treatment with drugs such as 5-fluorouracil, cisplatin, oxaliplatin, doxorubicin, regorafenib, and radiotherapy. By analyzing more than 140 papers from PubMed, Google Scholar, and SciFinder, we hope to find the therapeutic potential of CGA in improving cancer therapy. [ABSTRACT FROM AUTHOR]

Background: The Comprehensive Geriatric Assessment (CGA) records geriatric syndromes in a standardized manner, allowing individualized treatment tailored to the patient's needs and resources. Its use has shown a beneficial effect on the functional outcome and survival of geriatric patients. A recently published German S1 guideline for level 2 CGA provides recommendations for the use of a broad variety of different assessment instruments for each geriatric syndrome. However, the actual use of assessment instruments in routine geriatric clinical practice and its consistency with the guideline and the current state of literature has not been investigated to date. Methods: An online survey was developed by an expert group of geriatricians and sent to all licenced geriatricians (n = 569) within Germany. The survey included the following geriatric syndromes: motor function and self-help capability, cognition, depression, pain, dysphagia and nutrition, social status and comorbidity, pressure ulcers, language and speech, delirium, and frailty. Respondents were asked to report which geriatric assessment instruments are used to assess the respective syndromes. Results: A total of 122 clinicians participated in the survey (response rate: 21%); after data cleaning, 76 data sets remained for analysis. All participants regularly used assessment instruments in the following categories: motor function, self-help capability, cognition, depression, and pain. The most frequently used instruments in these categories were the Timed Up and Go (TUG), the Barthel Index (BI), the Mini Mental State Examination (MMSE), the Geriatric Depression Scale (GDS), and the Visual Analogue Scale (VAS). Limited or heterogenous assessments are used in the following categories: delirium, frailty and social status. Conclusions: Our results show that the assessment of motor function, self-help capability, cognition, depression, pain, and dysphagia and nutrition is consistent with the recommendations of the S1 guideline for level 2 CGA. Instruments recommended for more frequent use include the Short Physical Performance Battery (SPPB), the Montreal Cognitive Assessment (MoCA), and the WHO-5 (depression). There is a particular need for standardized assessment of delirium, frailty and social status. The harmonization of assessment instruments throughout geriatric departments shall enable more effective treatment and prevention of age-related diseases and syndromes. [ABSTRACT FROM AUTHOR]

Daoda Qi,1,&ast; Yang Chen,1,&ast; Chengyi Peng,1 Yuan Wang,2 Zihao Liang,2 Jingjing Guo,1 Yan Gu1 1Department of Geriatrics, The Second Hospital of Nanjing, Nanjing University of Chinese Medicine, Nanjing, People’s Republic of China; 2Clinical Research Center, The Second Hospital of Nanjing, Affiliated to Nanjing University of Chinese Medicine, Nanjing, People’s Republic of China&ast;These authors contributed equally to this workCorrespondence: Yan Gu; Jingjing Guo, Department of Geriatrics, The Second Hospital of Nanjing, Nanjing University of Chinese Medicine, Nanjing, People’s Republic of China, Tel +86 25 83626297, Email guyan703@foxmail.com; guojingjing0017@126.comBackground and Objective: Timely recognition of risk factors for early progression in older adult patients with COVID-19 is of great significance to the following clinical management. This study aims to analyze the risk factors and create a nomogram for early progression in older adult patients with COVID-19 in the Omicron era.Methods: A total of 272 older adults infected with COVID-19 admitted from December 2022 to February 2023 were retrospectively recruited. Risk factor selection was determined using the logistic and the least absolute shrinkage and selection operator (LASSO) regression. A nomogram was then created to predict early progression, followed by the internal validation and assessment of its performance through plotting the receiver operating characteristic (ROC), calibration, and decision curves.Results: A total of 83 (30.5%) older adult patients presented an early progression on chest CT after 3– 5 days of admission under standard initiate therapy. Six independent predictive factors were incorporated into the nomogram to predict the early progression, including CRP > 10 mg/L, IL-6 > 6.6 pg/mL, LDH > 245 U/L, CD4+ T-lymphocyte count < 400/μL, the Activities of Daily Living (ADL) score ≤ 40 points, and the Mini Nutritional Assessment Scale-Short Form (MNA-SF) score ≤ 7 points. The area under the curve (AUC) of the nomogram in discriminating older adult patients who had risk factors in the training and validation cohort was 0.857 (95% CI 0.798, 0.916) and 0.774 (95% CI 0.667, 0.881), respectively. The calibration and decision curves demonstrated a high agreement in the predicted and observed risks, and the acceptable net benefit in predicting the early progression, respectively.Conclusion: We created a nomogram incorporating highly available laboratory data and the Comprehensive Geriatric Assessment (CGA) findings that effectively predict early-stage progression in older adult patients with COVID-19 in the Omicron era.Keywords: early progression, CGA, nomogram, COVID-19, omicron era

Abstract Background Hyponatremia presents with symptoms considered age-associated in the elderly. We assess the change in Comprehensive Geriatric Assessment (CGA) parameters after hyponatremia improvement in hospitalized geriatric patients. Methods We took 100 hyponatremic and same number of eunatremic geriatric patients (> 60 years) who were comorbidity, presenting-complaints, and age-matched. Four CGA parameters were utilized, the new Hindi Mental State Examination (HMSE), Barthel’s index of activities of daily living (ADL), Timed up and go Test (TUG), and handgrip strength by hand dynamometer (HG). We analyzed these at admission and discharge, and their relationship with change in sodium levels. Results Average age was 68.1 ± 5.8 years, with males constituting 75%. The CGA parameters demonstrated worse values amongst the hyponatremia than the normonatremia group. Severe hyponatremia group showed worse CGA scores in comparison with moderate and mild. With improvement in sodium level, the improvements in ADL, TUG, and HMSE scores were greater in the hyponatremia group (8.8 ± 10.1, 2.2 ± 2.5, and 1.7 ± 2.3 respectively) in comparison to the normonatremia reference group (4.7 ± 9.0, 1 ± 2.0, and 0.7 ± 1.3 respectively, P

Background: Chromogranin A (CgA) seems to be involved in the pathophysiology of different neurodegenerative pathologies such as Alzheimer's disease (AD) and dementia with Lewy Bodies (DLB). CgA is present in the aggregates of amyloid plaques and in Lewy bodies but CgA also has a function in neuroinflammatory processes via microglia. Our objective was to determine if there is a difference in the CgA concentration in the cerebrospinal fluid (CSF) of AD and DLB patients and whether the CgA concentration can discriminate between the two diseases. Methods: Using the previously described AlphaLewyMA cohort, we included 117 patients with a CSF CgA assay: 15 control subjects (CS group), 64 DLB patients, 17 AD patients and 21 patients with both AD and probable DLB criteria (AD/DLB group). CgA concentration was assessed using the MSD platform. Results: CSF CgA was increased in the AD and AD/DLB groups compared with the DLB group (p = 0.0006 between AD and DLB, p = 0.0013 between AD/DLB and DLB). No significant difference in CgA concentration was found between DLB and CS. ROC curve analysis showed an area under the curve of 0.791 between AD and DLB. CgA concentrations were correlated with t‐Tau and P‐Tau regardless of the pathology (for Tau: p = 0.022 for AD; p < 0.0001 for DLB; p = 0.004 for AD/DLB; for P‐Tau: p = 0.032 for AD; p < 0.0001 for DLB; p = 0.0009 for AD/DLB). Aβ42 was positively correlated with CgA in the DLB group but not in the AD and AD/DLB groups (for DLB: p < 0.0001; for AD: p = 0.57; for AD/DLB: p = 0.58). Conclusions: CSF CgA concentrations are increased in AD but not in DLB and correlate with P‐Tau and Tau whatever the disease. These results suggest a link between tauopathy/neurodegeneration and CgA. [ABSTRACT FROM AUTHOR]

The food industry is seeking a stable, non-toxic red dye as a substitute for synthetic pigments. This can result from the reaction between 5-O-Caffeoylquinic acid (5-CQA) and tryptophan (TRP). This study explores the reaction kinetics under ultrasound conditions and investigates reaction parameters, such as pH, temperature, and reactants' concentrations, to accelerate the reaction. At the end of the reaction, the solution was either spray-dried or acidified to isolate the pigment, which was evaluated for its potential as a food dye. Using ultrasound at 40 °C led to a significant acceleration of the reaction that was completed in 8.5 h, marking a 300% improvement compared to literature. The caffeic acid, and not the quinic acid, moiety of 5-CQA seems to be partly responsible for the formation of the red pigment. The pH had a profound impact on the reaction rate, with an optimal value of pH = 9.5. Increased TRP concentrations led to increased reaction rates, while higher 5-CQA concentrations led to significant deviations from redness (a* value). The pigment, lacking significant antimicrobial activity, exhibited remarkable thermal stability (pH 3–9), delaying food oxidation and color deterioration. The results indicate that the reaction can be significantly accelerated by ultrasound, which will be useful for the scale-up of the process and giving the produced pigment the potential for use as an alternative to artificial coloring. [ABSTRACT FROM AUTHOR]

Strawberries are a commonly used agricultural product in the food industry. In the traditional production model, labor costs are high, and extensive picking techniques can result in food safety issues, like poor taste and fruit rot. In response to the existing challenges of low detection accuracy and slow detection speed in the assessment of strawberry fruit maturity in orchards, a CR-YOLOv9 multi-stage method for strawberry fruit maturity detection was introduced. The composite thinning network, CRNet, is utilized for target fusion, employing multi-branch blocks to enhance images by restoring high-frequency details. To address the issue of low computational efficiency in the multi-head self-attention (MHSA) model due to redundant attention heads, the design concept of CGA is introduced. This concept aligns input feature grouping with the number of attention heads, offering the distinct segmentation of complete features for each attention head, thereby reducing computational redundancy. A hybrid operator, ACmix, is proposed to enhance the efficiency of image classification and target detection. Additionally, the Inner-IoU concept, in conjunction with Shape-IoU, is introduced to replace the original loss function, thereby enhancing the accuracy of detecting small targets in complex scenes. The experimental results demonstrate that CR-YOLOv9 achieves a precision rate of 97.52%, a recall rate of 95.34%, and an mAP@50 of 97.95%. These values are notably higher than those of YOLOv9 by 4.2%, 5.07%, and 3.34%. Furthermore, the detection speed of CR-YOLOv9 is 84, making it suitable for the real-time detection of strawberry ripeness in orchards. The results demonstrate that the CR-YOLOv9 algorithm discussed in this study exhibits high detection accuracy and rapid detection speed. This enables more efficient and automated strawberry picking, meeting the public’s requirements for food safety.

For many years, coffee has maintained its place in Turkey as Turkish coffee (i.e., boiled, finely ground, unfiltered), and has become globally recognized. However, in recent years, especially for young people, filter coffee has become more popular, instead of Turkish coffee. As is generally known today, climatic conditions and processing techniques have an impact on the constituents of coffee. It is also thought that the finer grinding of Turkish coffee may affect the variability of the coffee contents. The mentioned popularity of the coffee beverage is also causing an increased interest in its nutritional and functional properties as well as its chemical constituents. Therefore, an analytical determination of the coffee ingredients is essential. Taste may be the most important criterion for coffee consumption for most consumers, but the health benefits should not be underestimated. Coffee has an antioxidant effect due to the phenolic compounds present, such as chlorogenic acid. It is also known to prevent cellular damage, which has led to increased interest in its antioxidant properties. The aim of this study was to investigate the total chlorogenic acids (CQAs), caffeine and antioxidant capacities of coffees commonly consumed in Turkey. For this purpose, three green and three roasted coffee samples of the Coffea arabica, Coffea canephora and Coffea liberica varieties were selected for this study and characterized. Different targeted mass spectrometry (LC-MS/MS) methods were developed to encompass the above-mentioned ingredients. The roasting process significantly increased the caffeine content in three roasted samples compared to green coffees. These values ranged from 1.2 g per 100 g dry mass (DM) in green coffees to 2.6 g per 100 g DM in roasted coffees. It could be shown that the most dominant CQA contained was 5-CQA. While the amount of CQA in green coffees is approximately 49–64%, it decreases down to 33–41%, by isomer conversion partly, to 4-CQA and 3-CQA in the roasting process. The highest value was found at 3.5 g/100 g DM. The highest value for the antioxidation capacity was 4.4 g ascorbic acid equivalents/100 g DM roasted coffee and the lowest value was 2.4 g/100 g DM in green coffee. This study was the first attempt to demonstrate the presence of different CQAs, caffeine and antioxidation capacity in coffee beans from the market in Turkey and needs to be followed up further with a larger sample size in future studies.

Simple Summary: Peptide receptor radionuclide therapy is a well-established therapy for the treatment of neuroendocrine tumors. Therapy typically consists of four cycles administered in 8–10 week intervals, resulting in an average treatment length of 8–10 months. Given the extensive treatment length, early identification of patients with disease progression could help to optimize disease management. Blood-based biomarkers such as chromogranin A, alkaline phosphatase (ALP), or the De Ritis ratio (aspartate aminotransferase/alanine aminotransferase) are continually assessed during treatment and represent a noninvasive and easily accessible source for the intratherapeutic monitoring of patients. Our exploratory analysis indicates that a considerable intratherapeutic increase in ALP may serve as a tool to identify patients who are at a higher risk of early disease progression after PRRT. If our results can be confirmed by other studies, these patients might benefit from intensified follow-up. Background: Pretherapeutic chromogranin A, alkaline phosphatase (ALP), or De Ritis ratio (aspartate aminotransferase/alanine aminotransferase) are prognostic factors in patients with metastatic neuroendocrine tumors (NET) undergoing peptide receptor radionuclide therapy (PRRT). However, their value for intratherapeutic monitoring remains unclear. We evaluated if changes in plasma markers during PRRT can help identify patients with unfavorable outcomes. Methods: A monocentric retrospective analysis of 141 patients with NET undergoing PRRT with [177Lu]Lu-DOTATOC was conducted. Changes in laboratory parameters were calculated by dividing the values determined immediately before each cycle of PRRT by the pretherapeutic value. Patients with low vs. high PFS were compared with the Wilcoxon rank-sum test. Results: Progression, relapse, or death after PRRT was observed in 103/141 patients. Patients with low PFS showed a significant relative ALP increase before the third (p = 0.014) and fourth (p = 0.039) cycles of PRRT. Kaplan–Meier analysis revealed a median PFS of 24.3 months (95% CI, 20.7–27.8 months) in patients with decreasing ALP values (Δ > 10%) during treatment, 12.5 months (95% CI, 9.2–15.8 months) in patients with increasing ALP values (Δ > 10%), and 17.7 months (95% CI, 13.6–21.8 months) with stable ALP values (Δ ± 10%). Conclusions: Based on these exploratory data, a rise in plasma ALP might indicate disease progression and should be interpreted cautiously during therapy. [ABSTRACT FROM AUTHOR]

Improper use of acetaminophen (APAP) will induce acute liver failure. This study is designed to investigate whether early growth response-1 (EGR1) participated in the promotion on liver repair and regeneration after APAP-induced hepatotoxicity provided by natural compound chlorogenic acid (CGA). APAP induced the nuclear accumulation of EGR1 in hepatocytes regulated by extracellular-regulated protein kinase (ERK)1/2. In Egr1 knockout (KO) mice, the liver damage caused by APAP (300 mg/kg) was more severe than in wild-type (WT) mice. Results of chromatin immunoprecipitation and sequencing (ChIP-Seq) manifested that EGR1 could bind to the promoter region in Becn1, Ccnd1, and Sqstm1 (p62) or the catalytic/modify subunit of glutamate-cysteine ligase (Gclc/Gclm). Autophagy formation and APAP-cysteine adduct (APAP-CYS) clearance were decreased in Egr1 KO mice administered with APAP. The EGR1 deletion reduced hepatic cyclin D1 expression at 6, 12, or 18 h post APAP administration. Meanwhile, the EGR1 deletion also decreased hepatic p62, Gclc and Gclm expression, GCL enzymatic activity, and glutathione (GSH) content and decreased nuclear factor erythroid 2-related factor 2 (Nrf2) activation and thus aggravated oxidative liver injury induced by APAP. CGA increased EGR1 nuclear accumulation; enhanced hepatic Ccnd1, p62, Gclc, and Gclm expression; and accelerated the liver regeneration and repair in APAP-intoxicated mice. In conclusion, EGR1 deficiency aggravated liver injury and obviously delayed liver regeneration post APAP-induced hepatotoxicity through inhibiting autophagy, enhancing liver oxidative injury, and retarding cell cycle progression, but CGA promoted the liver regeneration and repair in APAP-intoxicated mice via inducing EGR1 transcriptional activation. [ABSTRACT FROM AUTHOR]

Background: Hyponatremia presents with symptoms considered age-associated in the elderly. We assess the change in Comprehensive Geriatric Assessment (CGA) parameters after hyponatremia improvement in hospitalized geriatric patients. Methods: We took 100 hyponatremic and same number of eunatremic geriatric patients (> 60 years) who were comorbidity, presenting-complaints, and age-matched. Four CGA parameters were utilized, the new Hindi Mental State Examination (HMSE), Barthel's index of activities of daily living (ADL), Timed up and go Test (TUG), and handgrip strength by hand dynamometer (HG). We analyzed these at admission and discharge, and their relationship with change in sodium levels. Results: Average age was 68.1 ± 5.8 years, with males constituting 75%. The CGA parameters demonstrated worse values amongst the hyponatremia than the normonatremia group. Severe hyponatremia group showed worse CGA scores in comparison with moderate and mild. With improvement in sodium level, the improvements in ADL, TUG, and HMSE scores were greater in the hyponatremia group (8.8 ± 10.1, 2.2 ± 2.5, and 1.7 ± 2.3 respectively) in comparison to the normonatremia reference group (4.7 ± 9.0, 1 ± 2.0, and 0.7 ± 1.3 respectively, P < 0.05). Conclusion: Our study is the first utilizing HMSE to assess change in cognitive ability with improvement in serum sodium levels in the Indian elderly. Hyponatremic patients show worse baseline CGA parameters, and hyponatremia severity correlates with worse motor and cognitive function. Improvement in the serum sodium level improves the CGA parameters. Correction of hyponatremia in the geriatric age group significantly impacts life quality. [ABSTRACT FROM AUTHOR]

Carcinoid heart disease (CHD) is a serious complication for patients with neuroendocrine tumors (NETs), and early detection is crucial. We aimed to investigate N‐terminal pro‐brain natriuretic peptide (NT‐proBNP), chromogranin A (CgA), and plasma 5‐hydroxyindoleacetic acid (P‐5‐HIAA) as a screening tool for detection of CHD. We prospectively included patients with disseminated small intestinal NETs (SI‐NETs) and performed transthoracic echocardiography (TTE), questionnaires, and biochemical assessment of NT‐proBNP, CgA, and P‐5‐HIAA. The presence and severity of CHD was assessed using a scoring system based on echocardiographic characteristics. A total of 93 patients were included in the final analysis. Fifteen (16%) were diagnosed with CHD. The median NT‐proBNP (219 ng/L vs. 124 ng/L, p =.05), CgA (3930 pmol/L vs. 256 pmoL/L, p <.0001), and P‐5‐HIAA (1160 nmol/L vs. 210 nmoL/L, p <.0001) were significantly higher in patients with CHD compared to non‐CHD patients. For NT‐proBNP, the area under the receiver operating characteristic (AUROC) curve for detection of CHD was 0.67 (95% CI: 0.50–0.84), and at a 260 ng/L cutoff level, the sensitivity and specificity were 46% and 79%. For CgA, the AUROC was 0.91 (95% CI: 0.84–0.97), and at a cutoff level of 598 pmol/L, the sensitivity and specificity were 100% and 69%. For P‐5‐HIAA, the AUROC was 0.89 (95% CI: 0.80–0.98), and at a cutoff level of 752 nmol/L, the sensitivity and specificity were 92% and 85%. In conclusion, CgA and P‐5‐HIAA proved excellent markers of CHD while NT‐proBNP lacked the required diagnostic accuracy to be used as a screening tool. [ABSTRACT FROM AUTHOR]

As insurance plays a very crucial role in Indian financial system and its various components, a constant expansion of this sector is crucial for the growth of economy and benefit of its nationals. It is the right strategy and approach which make it easier for the insurance companies and the insurance advisors to stand out from their competitors and make the final sale. This paper highlights the scope of online Consumer-Generated Advertising (CGA) in Customer to Customer (C2C) marketplace in the context of insurance sector of India. The study is a qualitative one, using the tool of in-depth interviews. The study highlights the aspects of perceived 'usefulness', 'ease of use', 'trustworthiness' and 'attitude' in context of online CGA and finds out its impact on customer's 'intention to purchase' an insurance policy. It is found that, Consumer-Generated Advertising (CGA) in the form of WhatsApp and other online communications is the way forward, as in spite of the rapid technological progress, it is having the humane touch and understanding of the need of individual customers, and thereafter providing customers the most suitable insurance solution. [ABSTRACT FROM AUTHOR]

Collaborative edge-cloud features improve job scheduling. Cloud job scheduling is crucial. Pending delay completion. A cloud-edge mixed system replaced centralized cloud computing. Combining resource levels reduces terminal user service call latency. Decentralization, regionalization, and node dispersal autonomy increase ambiguity, unreliability, and instability. This paper will plan cloud-migrating tasks on edge devices or the cloud to achieve a global optimum. The objective of this research is to enhance the efficiency of job scheduling in cloud-fog edge environments through the integration of the Catastrophic Genetic Algorithm (CGA), a genetic algorithm inspired by natural evolution. Additionally, Berger's theory will be employed to develop a trust-enabled interaction framework based on blockchain technology. The CGA fitness function incorporates load balancing and reasonability in the coordination of services and scheduling of tasks, with the goal of maximizing performance. This article presents proposed improvements to the CGA, which involve the incorporation of mutation and crossover operators, roulette selection, and cataclysm. These changes aim to expand the search area and potentially discover schedules that are more optimal. The approach also effectively deals with the problem of premature convergence, guaranteeing ample time for the algorithm to comprehensively explore the solution space prior to reaching a final solution. The experimental findings indicate that the strategy put forward in this study yields a substantial reduction in task completion time, surpassing 97%. Furthermore, it effectively addresses the best local problem, hence showcasing competing options.

Objectives This retrospective analysis aims to determine the accuracy of medication histories undertaken during a Comprehensive Geriatric Assessment (CGA) (at the point of admission) in an inpatient setting within an acute hospital. The CGA can be completed by a range of healthcare professionals, which will be directly compared. Medication reviews are an integral part of the CGA, therefore the accuracy of the history is integral to the review. Methods A retrospective analysis of patient electronic records between October 2018 and February 2020 was conducted. The accuracy of medication histories recorded as part of the CGA completed by Advanced Nurse Practitioners, Advanced Pharmacist Practitioners and Doctors was compared. This was done using electronic records, any discrepancies were captured. Descriptive statistics were applied. Key findings Across the 50 patients and 421 medicines identified, there were 59 discrepancies; 24 (48%) patients were noted with at least one discrepancy in their medication history. An omission of current medication was the most common error (49%), followed by incorrect dose (19%) and a record of a medication not taken pre-admission (15%). The total number of prescribed medications was found to positively correlate with the rate of error (Pearson's correlation 0.455, P > 0.001). An independent t -test determined a significant difference between the rate of errors between pharmacists and nurses (t -test: −4.48, P < 0.001). Conclusions This study re-confirmed risks of medication errors when patients change care settings. This is more prevalent with higher numbers of medications, requiring greater care in instances of polypharmacy, particularly in frail patients. This can support risk stratification of patients. A difference in outcomes between pharmacists and nurses warrants further exploration to determine the reasons for this and structuring multi-disciplinary teams accordingly. Consideration to ensure that medication history taking is included as part of the Advanced Clinical Practitioner is important, particularly with the expansion of these roles in the UK. [ABSTRACT FROM AUTHOR]

The immersed boundary method (IBM) is widely used in simulating multiphase flows. DCU (Deep Computing Unit) acceleration device is a kind of GPU-like device, which is developed by GPU technology authorised by AMD. To achieve an efficient numerical simulation of two-phase flows, we develop a parallel DCU-based immersed boundary method using the MPI+HIP programming model. To solve the slow convergence problem caused by domain partitioning in multigrid methods, a coarse grid aggregation (CGA) technique is effectively employed. We adopt a nonblocking communication approach with HIP streams to overlap computation and communication. To validate the proposed method running on DCU clusters, we conducted two numerical experiments of droplet deformation in shear flow and the rising bubble. Performance tests show that a speedup of 181 times more than the CPU version can be achieved with 32 DCUs in a $ 1024\times 512\times 512 $ 1024 × 512 × 512 mesh grid. [ABSTRACT FROM AUTHOR]

Abstract Background Orthostatic Hypotension (OH) and malnutrition, are common health problems in elderly hypertensive patients. This study aimed to analyze the relationship between malnutrition and OH in elderly hypertensive patients. Methods This is a cross-sectional single-center study. All participants underwent a Comprehensive Geriatric Assessment (CGA), in which malnutrition was defined according to the Global Leadership Initiative on Malnutrition (GLIM) criteria based on four different methods of diagnosing muscle mass loss. Furthermore, the accuracy of these methods was verified by Receiver Operating Characteristic (ROC) analysis. Univariate and multivariate logistic regression analyses were used to identify risk factors for OH in elderly hypertensive patients. Results For GLIM criteria, when Fat-Free Mass Index (FFMI) was the gold standard for muscle mass loss, the Area Under ROC Curve (AUC) values for Upper Arm Circumference (UAC), Calf Circumference (CC), and Hand Grip Strength (HGS) were 0.784, 0.805, and 0.832, with moderate accuracy in diagnosing malnutrition. Multivariate analysis showed that females, Diabetes Mellitus (DM), diuretics, and malnutrition diagnosed by GLIM-UAC were risk factors for OH in elderly hypertensive patients. Conclusion Prompt detection of malnutrition in the elderly and attention to changes in UAC may be critical. Similarly, we should strengthen medication and disease management in elderly hypertensive patients.

Simple Summary: At Istituto Oncologico Veneto we are providing a geriatric assessment to all patients aged 70 years and older since 2003. Soft tissue sarcoma are really rare neoplasm and we, as a referral centre, evaluate a high volume of patients, so we decided to conduct this study to describe the geriatric multidisciplinary management and also the role the geriatric tools in the decision making and in assessing the prognosis. Background: Incidences of soft tissue sarcomas (STS) steadily increase with age. Yet, despite the high prevalence in advanced age, older patients (pts) are underrepresented in sarcoma clinical trials and evidence-based guidelines for chemotherapy are lacking. International oncological societies suggest using geriatric tools to evaluate older patients with cancer to optimise treatment indication. Comprehensive geriatric assessment (CGA) is a multidimensional assessment of older subjects, based on which pts can be classified as fit, vulnerable or frail. Onco-MPI (multidimensional prognostic index) is a CGA-based score which also considers tumour characteristics, classifying pts into three risk groups of death at one year: high-risk, intermediate-risk and low-risk. Methods: This is a single-centre retrospective study which aims at describing real-word management and outcomes of older pts with advanced stage STS and at assessing the ability of CGA and onco-MPI to predict survival in these pts. Consecutive pts with advanced stage STS aged 70 years or older and treated at the Istituto Oncologico Veneto from January 2009 to June 2020 were retrieved from a prospectively maintained database. Pts' demographics, CGA assessments and tumour characteristics were analysed. Statistical analysis was performed with R version 3.4.3 Results: Out of 101 pts, with a median age of 77 years, 76 received chemotherapy (75.3%), which was anthracycline-based for 46 pts (60.5%). Anthracyclines were used in a higher proportion in fit pts (58.9% fit vs. 45.1% vulnerable vs. 12.5% frail pts). Frail pts and pts in the onco-MPI high-risk group experienced a higher rate of chemotherapy-related toxicities. Median OS was 13.8 months (95% CI 11.3–17.7 months). According to CGA, the median OS was 19.53 months (95% CI 15.23–36.8) for fit pts, 12.83 months (95% CI 9.7–17.5) for vulnerable and 7.75 months (95% CI 2.73–30) for frail pts (p = 0.005). Onco-MPI confirmed a predictive value for 1-year survival with intermediate risk pts not reaching a median OS at 1 year, and high-risk pts having a median one-year OS of 11.5 months (95%CI 9.7–NA), p = 0.02. In multivariate analysis, onco-MPI and CGA were associated with survival (high risk onco-MPI: HR 5.5, 95%CI 1.25–24.7 p = 0.02; fitness at CGA HR 0.552 95% 0.314–0.973; p = 0.040) as well as chemotherapy use (HR 0.24, 95% CI 0.11–0.51, p < 0.005). Conclusions: Both CGA and onco-MPI retain prognostic value for survival in pts with metastatic STS. Pts frail/vulnerable at CGA and pts within the onco-MPI high risk category should be offered an oncogeriatric management approach in order to optimise treatment-related survival and reduce toxicity. [ABSTRACT FROM AUTHOR]

Spent coffee grounds (SCGs) have been extensively investigated as a feedstock to produce fuels, specialty chemicals and materials. Whilst a few reports have used cascade processes to generate several products from SCG, this work takes the novel approach of using integrated subcritical water extraction (SWE) and hydrothermal carbonisation (HTC) to derive three products: a bioactive extract, a protein isolate (SCG PI) and solid fuel. SWE and HTC processes were optimized producing an antioxidant rich extract, with the chlorogenic acid (CGA) content and antioxidant activity determined. The protein content was quantified via total amino acid analysis, giving the first SCG specific elemental nitrogen-to-protein conversion factor of 7.90. HTC was then performed on the residual solids from SWE, the protein extraction and the raw feedstock. This biorefinery approach gave higher quality products than previously reported in single product systems. For example, pretreatment reduced nitrogen in the hydrochar (N = 0.23% wt, HHV = 33.30 MJ/kg) relative to the control (3.03% wt, HHV = 31.31 MJ/kg). Limiting biorefinery processes to the pretreatment and HTC preferentially increased protein content (33.0% vs 16.9% wt) and yield (53.0% vs 23.9%) of the protein isolate, rendering a hydrochar with a higher yield and HHV compared with hydrochar derived following upstream SWE process (33.30 vs 26.92 MJ/kg, 16.3% vs 14.7%, respectively). This work goes towards the complete utilisation of SCGs within a biorefinery, highlighting the potential of subcritical water processing to produce commercially viable products across the value chain. [ABSTRACT FROM AUTHOR]

Aim: Comprehensive geriatric assessment (CGA) is a commonly used intervention for addressing the health needs of older people. Traditionally used for hospital inpatients, there is inconclusive evidence on its effectiveness in other settings. This overview of reviews aims to synthesize the current evidence regarding CGA models, their effectiveness, feasibility and acceptability in nonhospital settings., Methodology: The overview included systematic reviews with or without meta-analysis reporting on older adults (≥50 years) undergoing CGA in primary care facilities, outpatient care, community-based programs, long-term care settings and home-based care, excluding narrow high-risk groups or specific disease conditions. MEDLINE, Embase, Cochrane CENTRAL, PsychInfo and CINAHL, were searched up to August 2023, with blinded double screening and data extraction. Review quality was assessed using Assessing the Methodological Quality of Systematic Reviews-2., Results: Of 2574 identified articles, 22 systematic reviews (478 primary studies, >136 336 participants) met the inclusion criteria. Most reviews were of moderate to good quality, 73% were based in community or home settings, and in 50% of the reviews the participants were aged ≥65 years. They identified diverse CGA models without uniform definition or domains of assessment, most delivered by nurses and multidisciplinary teams, with variation of input duration (6-36 months) and poor reporting of frequency of assessment or follow-up duration. Most reviews found no effect of CGA on rates of admission to hospitals or long-term care facilities, quality of life or function. Of the 10 reviews that reported it as an outcome, only two found that CGA reduced mortality risk by 13% and 14% at longest follow-up. Of the three reviews reporting frailty as an outcome, two showed benefit, with one meta-analysis showing significant reduction in frailty (relative risk, 0.77 [95% CI, 0.64-0.93]). The acceptability of CGA is good among care providers, but limited information was found among patients. Facilitators for implementation identified include preexisting interprofessional working, skilled staff, better informed patients and focus on patient convenience. The evidence was exclusively from high-income countries., Conclusion: CGA models are highly heterogenous across domains, delivery, dosage and frequency. Most systematic reviews show little to no evidence of benefit of CGA on rates of hospitalization/long-term care admissions, functional ability and quality of life. However, CGA may have a positive effect on frailty and mortality, particularly in relatively vulnerable older people. Strategies to first identify high-risk individuals, followed by CGA, could lead to better outcomes. The lack of evidence on the effectiveness of CGA in low- and middle-income countries, despite the rapid increase in the number and proportion of older adults, means that trials in this area are urgently needed., Registration: Registered prospectively in the Open Science Framework Registry of reviews (No. ZB69F). Protocol can be accessed at: DOI: https://doi.org/10.17605/OSF.IO/ZB69F. No amendments to the protocol were made. Geriatr Gerontol Int 2025; ••: ••-••., (© 2025 The Author(s). Geriatrics & Gerontology International published by John Wiley & Sons Australia, Ltd on behalf of Japan Geriatrics Society.)

The purpose of this study was to examine the relationship between the number of program types and CGA in community gathering places to gain valid insights into the provision of programs in community gathering places. The study is longitudinal in nature and uses prospective cohort data from the Japan Gerontological Evaluation Study. The participants were 8758 older adults recruited from 28 cities and towns. The dependent variable was the CGA using the KCL. The independent variable was the number of program types in community gathering places. The participants were classified under four groups, namely, No Participation, One Type, Two Types, and More Than Three Types. The study performed linear regression analysis. The mean of the KCL scores by the number of types was 3.8 ± 3.3, 3.5 ± 3.0, 3.3 ± 2.9, and 3.2 ± 2.8 points for the four groups, respectively. CGA was better for two and three or more types of program participation. CGA at 3 years was better when the community gathering places implemented two or more types of program., Competing Interests: Declaration of Conflicting InterestsThe author(s) declared no potential conflicts of interest with respect to the research, authorship, and/or publication of this article.

For many years, coffee has maintained its place in Turkey as Turkish coffee (i.e., boiled, finely ground, unfiltered), and has become globally recognized. However, in recent years, especially for young people, filter coffee has become more popular, instead of Turkish coffee. As is generally known today, climatic conditions and processing techniques have an impact on the constituents of coffee. It is also thought that the finer grinding of Turkish coffee may affect the variability of the coffee contents. The mentioned popularity of the coffee beverage is also causing an increased interest in its nutritional and functional properties as well as its chemical constituents. Therefore, an analytical determination of the coffee ingredients is essential. Taste may be the most important criterion for coffee consumption for most consumers, but the health benefits should not be underestimated. Coffee has an antioxidant effect due to the phenolic compounds present, such as chlorogenic acid. It is also known to prevent cellular damage, which has led to increased interest in its antioxidant properties. The aim of this study was to investigate the total chlorogenic acids (CQAs), caffeine and antioxidant capacities of coffees commonly consumed in Turkey. For this purpose, three green and three roasted coffee samples of the Coffea arabica, Coffea canephora and Coffea liberica varieties were selected for this study and characterized. Different targeted mass spectrometry (LC-MS/MS) methods were developed to encompass the above-mentioned ingredients. The roasting process significantly increased the caffeine content in three roasted samples compared to green coffees. These values ranged from 1.2 g per 100 g dry mass (DM) in green coffees to 2.6 g per 100 g DM in roasted coffees. It could be shown that the most dominant CQA contained was 5-CQA. While the amount of CQA in green coffees is approximately 49–64%, it decreases down to 33–41%, by isomer conversion partly, to 4-CQA and 3-CQA in the roasting process. The highest value was found at 3.5 g/100 g DM. The highest value for the antioxidation capacity was 4.4 g ascorbic acid equivalents/100 g DM roasted coffee and the lowest value was 2.4 g/100 g DM in green coffee. This study was the first attempt to demonstrate the presence of different CQAs, caffeine and antioxidation capacity in coffee beans from the market in Turkey and needs to be followed up further with a larger sample size in future studies. [ABSTRACT FROM AUTHOR]

Introduction: The COVID-19 pandemic negatively affected the mental health of the Polish population. Students are the group with a high risk of developing mood disorders. The scientific data suggest the usefulness of biological substances as potential biomarkers of psychiatric disorders. Saliva is non-invasive, quick to obtain material and a promising fluid for measuring stress and depression severity. In this paper, we present the protocol of our study, which aims to assess the utility of concentrations of biological fluids as potential biomarkers of the stress response and risk of developing depression and anxiety disorders among students of Lublin. Materials and methods: Total number of 80 students were included in the study. Participants were asked to collect saliva samples simultaneously with responding to the questionnaires concerning their mental state. Afterwards, the samples were examined with an ELISA test to assess the level of fibroblast growth factor (FGF-2), cortisol and chromogranin A (CgA). The relationship between biomarkers and severity of stress, depression, anxiety will be examined. Results: Cortisol on its own is not a good material to investigate the stress reaction in saliva due to its variable levels among people during stressful situations. Scientific data indicate the usefulness of other biomarkers or their combination to indicate the potential risk of developing depression and/or anxiety. Conclusions: Introducing objective tests, helpful for mood symptoms recognition, would improve the prevention and diagnosis of psychiatric disorders in vulnerable populations. [ABSTRACT FROM AUTHOR]

Background: Orthostatic Hypotension (OH) and malnutrition, are common health problems in elderly hypertensive patients. This study aimed to analyze the relationship between malnutrition and OH in elderly hypertensive patients.Methods: This is a cross-sectional single-center study. All participants underwent a Comprehensive Geriatric Assessment (CGA), in which malnutrition was defined according to the Global Leadership Initiative on Malnutrition (GLIM) criteria based on four different methods of diagnosing muscle mass loss. Furthermore, the accuracy of these methods was verified by Receiver Operating Characteristic (ROC) analysis. Univariate and multivariate logistic regression analyses were used to identify risk factors for OH in elderly hypertensive patients.Results: For GLIM criteria, when Fat-Free Mass Index (FFMI) was the gold standard for muscle mass loss, the Area Under ROC Curve (AUC) values for Upper Arm Circumference (UAC), Calf Circumference (CC), and Hand Grip Strength (HGS) were 0.784, 0.805, and 0.832, with moderate accuracy in diagnosing malnutrition. Multivariate analysis showed that females, Diabetes Mellitus (DM), diuretics, and malnutrition diagnosed by GLIM-UAC were risk factors for OH in elderly hypertensive patients.Conclusion: Prompt detection of malnutrition in the elderly and attention to changes in UAC may be critical. Similarly, we should strengthen medication and disease management in elderly hypertensive patients. [ABSTRACT FROM AUTHOR]

Worldwide, falls and accompanying injuries are increasingly common, making their prevention and management a critical global challenge. The wealth of evidence to support interventions to prevent falls has recently (2022) been distilled in the first World Falls Guideline for Prevention and Management for Older Adults. The core of falls prevention includes (i) risk assessment and stratification; (ii) general recommendations on optimising physical function and mobility for all and (iii) offering a holistic, multidomain intervention to older adults at high risk of falls, in which the older adult's priorities, beliefs and resources are carefully considered. In recent decades, sustainable and adequately resourced falls prevention has proved challenging, although evidence suggests that suboptimal implementation of falls prevention is ineffective. Future research should focus on understanding the most successful approaches for implementation. To further optimise falls prevention, recent developments include technological innovation to identify and prevent falls, including exergaming. Further work is warranted to understand how to best incorporate the concepts of frailty and sarcopenia in falls prevention and management. This themed collection includes key articles in the field of falls prevention, covering several topics including risk factors, effective interventions, older adult's views, implementation issues and future perspectives. [ABSTRACT FROM AUTHOR]

Abstract Background Pre-treatment evaluation for sarcopenia is recommended in cancer patients. New screening tests that are less time-consuming and can identify patients who will potentially benefit from geriatric assessment are being developed; the G8 geriatric screening test is one such example. We aimed to investigate whether the G8 screening test can detect probable sarcopenia and is valid and reliable compared to a comprehensive geriatric assessment (CGA) in Turkish older adults with solid cancers. Methods We included solid cancer patients referred to a single center. Probable sarcopenia and abnormal CGA were defined as low handgrip strength. Cut-offs for handgrip strength in the Turkish population have been previously determined to be 32 kg for males and 22 kg for females and impairment in at least one of the CGA tests, respectively. The CGA tests comprised KATZ Basic Activities of Daily Living Scale Lawton–Brody Instrumental Activities of Daily Living Scale, Mini-Mental-State Examination Scale, Geriatric Depression Scale-15, and Mini-Nutritional Assessment Short Form. Receiver operating characteristic curve analyses evaluated the test’s predictive ability. Intra-rater and inter-rater reliabilities were assessed. Results The median age of the 76 patients included was 72 (65–91) years. There was a moderate correlation between handgrip strength and the G8 test total score. The sensitivity and specificity of the G8 test to detect probable sarcopenia alone (cut off score = 12.5) were 50 and 92%, respectively (AUC: 0.747; p

Abstract Introduction The treatment of elderly‐onset rheumatoid arthritis (RA) pursues the same objectives as in younger patients: to control the symptoms, to prevent structural damage, to preserve function, and to decrease excess mortality. The presence of co‐morbidities is more specific in elderly patients with RA, which raises therapeutic challenges for physicians. Comprehensive geriatric assessment (CGA) is an essential tool that examines factors affecting the course of disease and the outcome of treatment. CGA includes the patient's functional, physical, mental, emotional, pharmacotherapeutic, and socioeconomic status. This article aims to present CGA in the evaluation and management of frail elderly patient with RA. Patient & Result A case study of an 80‐year‐old man with RA and ILD is presented to describe the process of CGA and provides nutritional supplement therapy, patient education, and rehabilitation guidance to help patient progress from frailty to non‐frailty. Discussion The case revealed that CGA provides physicians with information on the reversible area of frailty and the leading cause of deterioration in EORA.

Abstract Objective Our aim was to analyse the chromogranin A-derived peptides vasostatin and catestatin in serum from wild boar (Sus scrofa) captured in a corral trap. Acute capture-related stress quickly leads to a release of adrenalin and noradrenalin, but these hormones have a short half-life in blood and are difficult to measure. Chromogranin A (CgA), a glycoprotein which is co-released with noradrenalin and adrenalin, is relatively stable in circulation and the CgA-derived peptides catestatin and vasostatin have been measured in domestic species, but not yet in wildlife. Results Vasostatin and catestatin could be measured and the median (range) serum concentrations were 0.91 (0.54–2.86) and 0.65 (0.35–2.62) nmol/L, respectively. We conclude that the CgA-derived peptides vasostatin and catestatin can be measured in wild boar serum and may thus be useful as biomarkers of psychophysical stress.

Light, as an important environmental signal for plant growth and development, has been reported to be involved in the metabolism of phenylpropane. However, the light-induced phenylpropane biosynthesis mechanism in Chrysanthemum morifolium Ramat., especially flavonoid and chlorogenic acid (CGA) metabolism, is not clear. In this study, we found the flower phenotype of chrysanthemum 'HangBaiJu' became lighter after bagging. Besides, the content of main active ingredients, such as Luteolin-7-O-glucoside (Lu-7-O-G), Diosmetin-7-O-glucoside (Di-7-O-G), Apigenin-7-O-glucoside, Apigenin and CGA, was significantly decreased. Comparative transcriptome analysis (pairwise comparison, WGCNA, and k-means clustering) revealed that the expression of structural genes, such as CmPAL , CmCHS1/2, CmF3'H, CmFNS and CmHQT, was notably declined under dark conditions. Meanwhile, 'bridge proteins' CmMYB3/6/16 and signal transduction factors CmBBX20/22.2/22.3 were identified as key regulatory factors in the light-mediated synthesis of flavonoids and CGA in chrysanthemum. Among that, CmBBX20 could promote the accumulation of flavonoids and CGA by directly binding to the G-box core sequences of downstream target genes based on transient overexpression and Y1H assays. Overall, the preliminary molecular mechanism of BBXs and MYBs coordinately regulating the accumulation of flavonoids and CGA in chrysanthemum under different light inductions has been clarified, which provided a theoretical basis for the molecular breeding and quality improvement of chrysanthemum. • The content of Lu-7-O-G, Di-7-O-G, Ap-7-O-G, Apigenin and CGA was correlated with the color of chrysanthemum flowers. • CmPAL, CmCHS1/2, CmF3'H, CmFNS and CmHQT are key structural genes in the flavonoid and CGA synthesis in response to light. • CmBBX20 promoted the light-induced synthesis of flavonoids and CGA by binding to the G-box core sequences of t arget genes. [ABSTRACT FROM AUTHOR]

[Display omitted] • CGA alleviated retinal inflammation in DR progression. • CGA reduced TNF α release from hyperglycemia-treated Müller cells. • CGA reversed the adherent of PBMC on TNF α -stimulated retinal endothelial cells. • CGA reduced VCAM1 and ICAM1 expression in TNF α- treated HRECs. • CGA hindered the interaction between TNFR1 and TNF α by directly binding to TNFR1. As a prominent complication of diabetes mellitus (DM) affecting microvasculature, diabetic retinopathy (DR) originates from blood-retinal barrier (BRB) damage. Natural polyphenolic compound chlorogenic acid (CGA) has already been reported to alleviate DR. This study delves into the concrete mechanism of the CGA-supplied protection against DR and elucidates its key target in retinal endothelial cells. DM in mice was induced using streptozotocin (STZ). CGA mitigated BRB dysfunction, leukocytes adhesion and the formation of acellular vessels in vivo. CGA suppressed retinal inflammation and the release of tumor necrosis factor- α (TNF α) by inhibiting nuclear factor kappa-B (NF κ B). Furthermore, CGA reduced the TNF α -initiated adhesion of peripheral blood mononuclear cell (PBMC) to human retinal endothelial cell (HREC). CGA obviously decreased the TNF α -upregulated expression of vascular cell adhesion molecule-1 (VCAM1) and intercellular adhesion molecule-1 (ICAM1), and abrogated the TNF α -induced NF κ B activation in HRECs. All these phenomena were reversed by overexpressing type 1 TNF receptor (TNFR1) in HRECs. The CGA-provided improvement on leukocytes adhesion and retinal inflammation was disappeared in mice injected with an endothelial-specific TNFR1 overexpression adeno-associated virus (AAV). CGA reduced the interaction between TNF α and TNFR1 through binding to TNFR1 in retinal endothelial cells. In summary, excepting reducing TNF α expression via inhibiting retinal inflammation, CGA also reduced the adhesion of leukocytes to retinal vessels through decreasing VCAM1 and ICAM1 expression via blocking the TNF α -initiated NF κ B activation by targeting TNFR1 in retinal endothelial cells. All of those mitigated retinal inflammation, ultimately alleviating BRB breakdown in DR. [ABSTRACT FROM AUTHOR]