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cute Hepatopancreatic Necrosis Syndrome -AHPNSof cultured shrimp was first detected in China in 2009 and caused huge damage to shrimp farming in many countries including Vietnam. This study investigates the ability to inhibit Vibrio parahaemolyticuswhich causes hepatopancreatic necrosis of some Bacillusstrains. V. parahaemolyticusNT7 of this research was isolated from a white leg shrimp sample with hepatopancreatic necrosis in Ninh Thuan province and identified by biochemical methods. By the cross-steak and well-diffusion methods, the selected strain Bacillus polyfermenticusF27 showsthe largest diameter of 18.50 mm resistance to V.parahaemolyticus NT7.B. polyfermenticus F27strain caninhibitV. parahaemolyticus NT7. Besides, B. polyfermenticusF27 inhibits V. parahaemolyticusNT7 with co-cultured experiment and does not cause hemolysis. It is also safe for white leg shrimp seed with a 100% survival rate of the experimental treatments. The result of LD50 examination when infecting V.parahaemolyticus NT7 to white leg shrimp seed is 105CFU/ml. Through the host protectioncapability assessment of B. polyfermenticusF27, we found that itcanprotect white leg shrimp seed from V. parahaemolyticus.The findings show that strains of B. polyfermenticusF27 have the potential to produce probiotics for controland preventionof EMS/AHPNS of shrimps.