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Background. In order to fully reveal the advantages of a cultivar and use it effectively in production, it is necessary to ensure high sowing and varietal qualities of seeds, which requires improvement in breeding and seed production. Biotechnological methods are increasingly used in initial seed production. High efficiency was shown by the method of electrophoresis of seed storage proteins – prolamins. The aim of this study was to assess the biotype composition and allelic state of the prolamin-coding loci in the new spring oat cultivar ‘Tobolyak’ for its further use in initial seed production.Materials and methods. In 2021, 1519 families of cv. ‘Tobolyak’ were studied using the method of native electrophoresis. Three grains from each family were selected for analyses by random sampling. Electrophoresis was carried out in vertical plates of 13.2% polyacrylamide gel at a constant voltage of 500 V for 4.5–5.0 hours.Results and conclusion. The study of cv. ‘Tobolyak’ families resulted in identifying 13 types of avenin patterns with a frequency of occurrence from 96.36 to 0.07%. A new block of avenin components controlled by the allele of the Avn B locus was described. Number 8 was assigned to this block. It was established that cv. ‘Tobolyak’ had been developed by crossing a minor biotype of cv. ‘Taezhnik’ (Avn 4.4.2) with the first biotype of cv. ‘Orion’ (Avn 2.8.2). The basis of cv. ‘Tobolyak’ was one genotype with the Avn 4.8.2 avenin formula. The remaining biotypes arose as a result of crossing the biotypes of cvs. ‘Taezhnik’ and ‘Orion’ as well as through mechanical contamination. To maintain the genetic stability of cv. ‘Tobolyak’, it is recommended to use only families of the first biotype in the process of original seed production.

Molecular and biochemical markers are used to analyze the intraspecific genetic diversity of crops. Prolamincoding loci are highly effective for assessing this indicator. On the basis of the Laboratory of Varietal Seed Identification of the State Agrarian University of the Northern Trans-Urals, 18 varieties of common oat included in the State Register of Selection Achievements in the Tyumen Region from the 1930s to 2019 were studied by electrophoresis in 2018–2019. The aim of the work was to study the dynamics of the genetic diversity of oat va rieties at avenin-coding loci. For the analysis, 100 grains of each variety were used. Electrophoresis was carried out in vertical plates of 13.2 % polyacrylamide gel at a constant vol tage of 500 V for 4.0–4.5 h. It was found that 44.4 % of the varieties are heterogeneous, each consisting of two biotypes. For three loci, 20 alleles were identified, 10 of which were detected for the first time. The allele frequency of avenin-coding loci varied with time. In the process of variety exchange, alleles that are characteristic of varieties of non-Russian origin were replaced by alleles present in domestic varieties and then in the varieties developed by local breeding institutions. The following alleles had the highest frequency in Tyumen varieties: Avn A4(50.0 %), A2(25.0 %), Avn B4(50.0 %), Bnew6(37.5 %), Avn C1(37.5 %), C2 and C5(25.0 %). These alleles are of great value as markers of agronomically and adaptively important characters for the region in question. The amount of genetic diversity of oats varied with time from 0.33 in 1929–1950 to up to 0.75 in 2019. The high value of genetic diversity in modern breeding varieties of the Scientific Research Institute of Agriculture of the Northern Trans-Urals and an increase in this indicator over the past 20 years are associated with the use of genetically heterogeneous source material in the breeding process. This allowed obtaining varieties with high adaptive potentials in the natural climatic conditions of the region.

Background. In the environments of Western Siberia, oat is one of the main cultivated cereals. Six varieties of common oat are included in the State Register for Tyumen Province: 'Perona', 'Megion', 'Tyumensky Golozerny', 'Talisman', 'Otrada' and 'Foma'. Currently, not only traditional methods of breeding are used for the development of new cultivars of intensive type in Tyumen Province, but also biochemical markers - prolamins. The aim of the research was to study the component composition of avenins and allelic status of avenin-coding loci in common oat varieties cultivated in Tyumen Province as well as to identify alleles specific to the region.Materials and methods. For laboratory analysis we used 100 kernels selected at random from each of the oat varieties. Electrophoretic separation of avenin was carried out on vertical plates of polyacrylamide gel.Results and conclusion. The varieties 'Perona', 'Talisman', 'Foma' and 'Tyumensky Golozerny' were found to be homogeneous in their avenin component composition, while 'Megion' and 'Otrada' included two biotypes with a 2:1 ratio. Their heterogeneity is determined by the presence of two allelic variants controlled by the Avn A locus. Clustering by the UPGMA method showed that the studied varieties were divided into two clusters. The first cluster included 'Tyumensky Golozerny' and 'Megion', while the second harbored 'Perona', 'Talisman', 'Otrada' and 'Foma'. The genetic distance between the varieties 'Talisman' and 'Perona' was zero, and their avenin formulas coincided: Avn A4 B4 C2. The identity of their storage protein spectra is due to the fact that these allelic variants of prolamin component blocks are linked to valuable economic and adaptive features. Blocks of the components А4, В4, С1 and С2 prevail in the frequency of occurrence in the spectra of the investigated varieties. These blocks can act as markers of gene associations with adaptive significance and economic value. Thus, when performing breeding work in Tyumen Province, it is necessary to pay attention to oat varieties whose prolamin spectra contain the aforementioned blocks of avenin components.