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1. Tagging of water masses with covariance of trace metals and prokaryotic taxa in the Southern Ocean

Author

Rui Zhang, Stéphane Blain, Corentin Baudet, Hélène Planquette, Frédéric Vivier, Philippe Catala, Olivier Crispi, Audrey Guéneuguès, Barbara Marie, Pavla Debeljak, and Ingrid Obernosterer

Subjects
Oceanography, GC1-1581
Abstract

Abstract Marine microbes are strongly interrelated to trace metals in the ocean. How the availability of trace metals selects for prokaryotic taxa and the potential feedback of microbial processes on the trace metal distribution in the ocean remain poorly understood. We investigate here the potential reciprocal links between diverse prokaryotic taxa and iron (Fe), manganese (Mn), copper (Cu), and nickel (Ni) as well as apparent oxygen utilization (AOU) across 12 well‐defined water masses in the Southern Indian Ocean (SWINGS—South West Indian Ocean GEOTRACES GS02 Section cruise). Applying partial least square regression (PLSR) analysis, we show that the water masses are associated with particular latent vectors that are a combination of the spatial distribution of prokaryotic taxa, trace elements, and AOU. This approach provides novel insights on the potential interactions between prokaryotic taxa and trace metals in relation to organic matter remineralization in distinct water masses of the ocean.

Published
2024
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2. Comparative Thermophysiology of Marine Synechococcus CRD1 Strains Isolated From Different Thermal Niches in Iron-Depleted Areas

Author

Mathilde Ferrieux, Louison Dufour, Hugo Doré, Morgane Ratin, Audrey Guéneuguès, Léo Chasselin, Dominique Marie, Fabienne Rigaut-Jalabert, Florence Le Gall, Théo Sciandra, Garance Monier, Mark Hoebeke, Erwan Corre, Xiaomin Xia, Hongbin Liu, David J. Scanlan, Frédéric Partensky, and Laurence Garczarek

Subjects
marine picocyanobacteria, Synechococcus, CRD1, thermotype, temperature adaptation, Microbiology, QR1-502
Abstract

Marine Synechococcus cyanobacteria are ubiquitous in the ocean, a feature likely related to their extensive genetic diversity. Amongst the major lineages, clades I and IV preferentially thrive in temperate and cold, nutrient-rich waters, whilst clades II and III prefer warm, nitrogen or phosphorus-depleted waters. The existence of such cold (I/IV) and warm (II/III) thermotypes is corroborated by physiological characterization of representative strains. A fifth clade, CRD1, was recently shown to dominate the Synechococcus community in iron-depleted areas of the world ocean and to encompass three distinct ecologically significant taxonomic units (ESTUs CRD1A-C) occupying different thermal niches, suggesting that distinct thermotypes could also occur within this clade. Here, using comparative thermophysiology of strains representative of these three CRD1 ESTUs we show that the CRD1A strain MITS9220 is a warm thermotype, the CRD1B strain BIOS-U3-1 a cold temperate thermotype, and the CRD1C strain BIOS-E4-1 a warm temperate stenotherm. Curiously, the CRD1B thermotype lacks traits and/or genomic features typical of cold thermotypes. In contrast, we found specific physiological traits of the CRD1 strains compared to their clade I, II, III, and IV counterparts, including a lower growth rate and photosystem II maximal quantum yield at most temperatures and a higher turnover rate of the D1 protein. Together, our data suggests that the CRD1 clade prioritizes adaptation to low-iron conditions over temperature adaptation, even though the occurrence of several CRD1 thermotypes likely explains why the CRD1 clade as a whole occupies most iron-limited waters.

Published
2022
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3. The Role of the Glyoxylate Shunt in the Acclimation to Iron Limitation in Marine Heterotrophic Bacteria

Author

Coco Koedooder, Audrey Guéneuguès, Rémy Van Geersdaële, Valérie Vergé, François-Yves Bouget, Yannick Labreuche, Ingrid Obernosterer, and Stéphane Blain

Subjects
iron, Fe-limitation, Photobacterium angustum S14, glyoxylate shunt, isocitrate lyase, Science, General. Including nature conservation, geographical distribution, QH1-199.5
Abstract

Iron (Fe) is an essential element for marine microbial growth but is present in trace amounts (

Published
2018
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4. Vertical flux of trace elements associated with lithogenic and biogenic carrier phases in the Southern Ocean

Author

Stephane Blain, Hélène Planquette, Ingrid Obernosterer, audrey Guéneuguès, Laboratoire d'Océanographie Microbienne (LOMIC), Institut national des sciences de l'Univers (INSU - CNRS)-Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS)-Observatoire océanologique de Banyuls (OOB), Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS), Laboratoire des Sciences de l'Environnement Marin (LEMAR) (LEMAR), Institut de Recherche pour le Développement (IRD)-Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER)-Université de Brest (UBO)-Institut Universitaire Européen de la Mer (IUEM), and Institut de Recherche pour le Développement (IRD)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Brest (UBO)-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche pour le Développement (IRD)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Brest (UBO)-Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS)

Subjects
Atmospheric Science, Global and Planetary Change, faecal pellets, [SDU]Sciences of the Universe [physics], fungi, Environmental Chemistry, trace element, export, trace metal, General Environmental Science, southern ocean, diatoms
Abstract

International audience; Trace elements (TE) are tracers of multiple biotic and abiotic processes in the ocean and some of them are essential for marine life. Vertical export by particles is a major removal process of a large fraction of TE from the surface ocean. However, the seasonal export dynamics and its controlling factors, critical for the understanding of the internal TE cycling, remain poorly constrained. Here, we report and discuss the seasonal export of 15 TE in sinking particles collected by a sediment trap deployed in a highly productive region of the Southern Ocean. Basalt material was the main carrier phase for the export flux of 9 TE, and its dynamic was characterised by a strong decrease over time. TE export driven by biological carriers such as diatom spores and vegetative cells added pulsed seasonal dynamics to the lithogenic signal, while the contribution of fecal pellets was less variable over the season. For each TE, we were able to decipher the biological carrier phases that represent the most dominant export pathway. We discuss this partitioning with regards to the known metabolic functions of the different trace metals or TE of biological interest.

Published
2022
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5. The interplay between iron limitation, light, and carbon in the proteorhodopsin containing Photobacterium angustum S14

Author

Coco Koedooder, Ingrid Obernosterer, Rémy Van Geersdaële, Audrey Guéneuguès, Stéphane Blain, François-Yves Bouget, Laboratoire d'Océanographie Microbienne (LOMIC), Centre National de la Recherche Scientifique (CNRS)-Sorbonne Université (SU)-Institut national des sciences de l'Univers (INSU - CNRS)-Observatoire océanologique de Banyuls (OOB), and Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS)

Subjects
0301 basic medicine, Rhodopsin, proteorhodopsin, Iron, 030106 microbiology, Applied Microbiology and Biotechnology, Microbiology, Photoheterotroph, Respiratory electron transport chain, 03 medical and health sciences, Gene expression, Rhodopsins, Microbial, [SDU.STU.OC]Sciences of the Universe [physics]/Earth Sciences/Oceanography, Regulation of gene expression, Photobacterium angustum S14, Proteorhodopsin, Ecology, Phototroph, biology, Photobacterium, Photoheterotrophy, Fe-limitation, biology.organism_classification, [SDV.MP.BAC]Life Sciences [q-bio]/Microbiology and Parasitology/Bacteriology, Carbon, 030104 developmental biology, 13. Climate action, biology.protein, Biophysics, [SDE.BE]Environmental Sciences/Biodiversity and Ecology, Intracellular
Abstract

Iron (Fe) limitation is known to affect heterotrophic bacteria within the respiratory electron transport chain, therefore strongly impacting the overall intracellular energy production. We investigated whether the gene expression pattern of the light-sensitive proton pump, proteorhodopsin (PR), is influenced by varying light, carbon and Fe concentrations in the marine bacterium Photobacterium angustum S14 and whether PR can alleviate the physiological processes associated with Fe starvation. Our results show that the gene expression of PR increases as cells enter the stationary phase, irrespective of Fe-replete or Fe-limiting conditions. This upregulation is coupled to a reduction in cell size, indicating that PR gene regulation is associated with a specific starvation-stress response. We provide experimental evidence that PR gene expression does not result in an increased growth rate, cell abundance, enhanced survival or ATP concentration within the cell in either Fe-replete or Fe-limiting conditions. However, independent of PR gene expression, the presence of light did influence bacterial growth rates and maximum cell abundances under varying Fe regimes. Our observations support previous results indicating that PR phototrophy seems to play an important role within the stationary phase for several members of the Vibrionaceae family, but that the exact role of PR in Fe limitation remains to be further explored.

Published
2020
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6. Effects of iron limitation on growth and carbon metabolism in oceanic and coastal heterotrophic bacteria

Author

Ingrid Obernosterer, Aurélie Devez, Annick Schaumann, Marion Fourquez, Thierry Jouenne, Stéphane Blain, Audrey Guéneuguès, Laboratoire d'Océanographie Microbienne (LOMIC), Observatoire océanologique de Banyuls (OOB), Université Pierre et Marie Curie - Paris 6 (UPMC)-Centre National de la Recherche Scientifique (CNRS)-Université Pierre et Marie Curie - Paris 6 (UPMC)-Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS), Hydrosciences Montpellier (HSM), Institut national des sciences de l'Univers (INSU - CNRS)-Institut de Recherche pour le Développement (IRD)-Université Montpellier 2 - Sciences et Techniques (UM2)-Université de Montpellier (UM)-Centre National de la Recherche Scientifique (CNRS), Polymères Biopolymères Surfaces (PBS), Institut national des sciences appliquées Rouen Normandie (INSA Rouen Normandie), Institut National des Sciences Appliquées (INSA)-Normandie Université (NU)-Institut National des Sciences Appliquées (INSA)-Normandie Université (NU)-Institut de Chimie du CNRS (INC)-Institut Normand de Chimie Moléculaire Médicinale et Macromoléculaire (INC3M), Institut de Chimie du CNRS (INC)-École Nationale Supérieure d'Ingénieurs de Caen (ENSICAEN), Normandie Université (NU)-Normandie Université (NU)-Institut national des sciences appliquées Rouen Normandie (INSA Rouen Normandie), Institut National des Sciences Appliquées (INSA)-Normandie Université (NU)-Institut National des Sciences Appliquées (INSA)-Université Le Havre Normandie (ULH), Normandie Université (NU)-Université de Rouen Normandie (UNIROUEN), Normandie Université (NU)-Centre National de la Recherche Scientifique (CNRS)-Université de Caen Normandie (UNICAEN), Normandie Université (NU)-École Nationale Supérieure d'Ingénieurs de Caen (ENSICAEN), Normandie Université (NU)-Université Le Havre Normandie (ULH), Normandie Université (NU)-Centre National de la Recherche Scientifique (CNRS), Centre National de la Recherche Scientifique (CNRS)-Sorbonne Université (SU)-Institut national des sciences de l'Univers (INSU - CNRS)-Observatoire océanologique de Banyuls (OOB), Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS), Institut de Recherche pour le Développement (IRD)-Université Montpellier 2 - Sciences et Techniques (UM2)-Institut national des sciences de l'Univers (INSU - CNRS)-Centre National de la Recherche Scientifique (CNRS), Université de Rouen Normandie (UNIROUEN), Institut National des Sciences Appliquées (INSA)-Normandie Université (NU)-Institut National des Sciences Appliquées (INSA)-Institut Normand de Chimie Moléculaire Médicinale et Macromoléculaire (INC3M), Université de Caen Normandie (UNICAEN), Normandie Université (NU)-Normandie Université (NU)-École Nationale Supérieure d'Ingénieurs de Caen (ENSICAEN), Normandie Université (NU)-Institut national des sciences appliquées Rouen Normandie (INSA Rouen Normandie), Institut National des Sciences Appliquées (INSA)-Normandie Université (NU)-Institut National des Sciences Appliquées (INSA)-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS)-Université de Caen Normandie (UNICAEN), Normandie Université (NU)-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS), and Institut national des sciences de l'Univers (INSU - CNRS)-Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS)-Observatoire océanologique de Banyuls (OOB)

Subjects
0303 health sciences, biology, 030306 microbiology, Catabolism, [SDV]Life Sciences [q-bio], fungi, Heterotroph, Metabolism, Aquatic Science, Oceanography, biology.organism_classification, Carbon cycle, Citric acid cycle, 03 medical and health sciences, Marine bacteriophage, Biochemistry, Environmental chemistry, 14. Life underwater, Alteromonas macleodii, ComputingMilieux_MISCELLANEOUS, Bacteria, 030304 developmental biology
Abstract

We investigated the metabolic response to iron (Fe) limitation of two bacterial strains of Alteromonas macleodii , isolated from a coastal and an oceanic marine environment. Bacteria were grown under Fe-limited and Fe-replete conditions, and comparative analyses of cellular properties and total proteomes were conducted. Respiration was reduced by a factor of two in both strains, but the growth rate of the oceanic strain was less affected by Fe limitation (reduced by 1.2-fold) than the coastal strain (reduced by 2-fold). Fe limitation led to significant changes in the expression of several key enzymes associated with carbon catabolism, specifically those involved in the citric acid cycle, glycolysis, and oxidative phosphorylation. The strain-specific overall responses to Fe limitation were in part reflected in different metabolic strategies of the carbon metabolism and energy acquisition. Our study provides novel insights on how Fe limitation can affect bacterial heterotrophic metabolism, and how this could influence the coupling of the Fe and carbon cycles in the ocean.

Published
2014
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7. Central role for ferritin in the day/night regulation of iron homeostasis in marine phytoplankton

Author

Emmanuel Lesuisse, Audrey Guéneuguès, Robert Sutak, Christophe Six, Stéphane Blain, Thibaut Léger, Philippe Schatt, Chris Bowler, Hugo Botebol, François-Yves Bouget, Valerie M. K. Verge, Joe Morrissey, Jean-Michel Camadro, Jean-Claude Lozano, Amos Kirilovsky, Laboratoire d'Océanographie Microbienne (LOMIC), Centre National de la Recherche Scientifique (CNRS)-Université Pierre et Marie Curie - Paris 6 (UPMC)-Observatoire océanologique de Banyuls (OOB), Université Pierre et Marie Curie - Paris 6 (UPMC)-Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS), Institut Jacques Monod (IJM (UMR_7592)), Université Paris Diderot - Paris 7 (UPD7)-Centre National de la Recherche Scientifique (CNRS), Department of Parasitology, Faculty of Science, Charles University [Prague] (CU), MArine Phototrophic Prokaryotes (MAPP), Adaptation et diversité en milieu marin (AD2M), Station biologique de Roscoff [Roscoff] (SBR), Université Pierre et Marie Curie - Paris 6 (UPMC)-Centre National de la Recherche Scientifique (CNRS)-Université Pierre et Marie Curie - Paris 6 (UPMC)-Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS)-Station biologique de Roscoff [Roscoff] (SBR), Université Pierre et Marie Curie - Paris 6 (UPMC)-Centre National de la Recherche Scientifique (CNRS)-Université Pierre et Marie Curie - Paris 6 (UPMC)-Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS), Observatoire océanologique de Banyuls (OOB), Université Pierre et Marie Curie - Paris 6 (UPMC)-Centre National de la Recherche Scientifique (CNRS), Institut de biologie de l'ENS Paris (IBENS), Centre National de la Recherche Scientifique (CNRS)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Département de Biologie - ENS Paris, École normale supérieure - Paris (ENS Paris), Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-École normale supérieure - Paris (ENS Paris), Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), NASA Goddard Space Flight Center (GSFC), French National Research Agency 'PhytoIron' Grant 11BSV7 018 02, Institut National des Sciences de l’Univers (CNRS), Czech Science Foundation Grant 13-25349S, Project BIOCEV, CZ.1.05/1.1.00/02.0109, European Project: Diatomite, Institut de biologie de l'ENS Paris (UMR 8197/1024) (IBENS), Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Département de Biologie - ENS Paris, Procaryotes Phototrophes Marins = MArine Phototrophic Prokaryotes (MAPP), Département de Biologie - ENS Paris, École normale supérieure - Paris (ENS-PSL), Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-École normale supérieure - Paris (ENS-PSL), Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), Charles University [Prague], Adaptation et diversité en milieu marin (ADMM), Université Pierre et Marie Curie - Paris 6 (UPMC)-Centre National de la Recherche Scientifique (CNRS)-Station biologique de Roscoff [Roscoff] (SBR), Université Pierre et Marie Curie - Paris 6 (UPMC)-Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS)-Université Pierre et Marie Curie - Paris 6 (UPMC)-Centre National de la Recherche Scientifique (CNRS)-Station biologique de Roscoff [Roscoff] (SBR), and École normale supérieure - Paris (ENS Paris)-École normale supérieure - Paris (ENS Paris)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)

Subjects
[SDV.BIO]Life Sciences [q-bio]/Biotechnology, Light, Nitrogen assimilation, Iron, Blotting, Western, Photosynthesis, Mass Spectrometry, Ostreococcus, Iron-Binding Proteins, Phytoplankton, Chemical Precipitation, Homeostasis, [SDV.BBM]Life Sciences [q-bio]/Biochemistry, Molecular Biology, Seawater, 14. Life underwater, Diel vertical migration, Multidisciplinary, Microbial Viability, biology, Ecology, ferritin, fungi, Iron-binding proteins, Biological Sciences, biology.organism_classification, Circadian Rhythm, Ferritin, Kinetics, circadian, [SDV.MP]Life Sciences [q-bio]/Microbiology and Parasitology, Gene Expression Regulation, 13. Climate action, Ferritins, biology.protein, Transcriptome
Abstract

International audience; In large regions of the open ocean, iron is a limiting resource for phytoplankton. The reduction of iron quota and the recycling of internal iron pools are among the diverse strategies that phytoplankton have evolved to allow them to grow under chronically low ambient iron levels. Phytoplankton species also have evolved strategies to cope with sporadic iron supply such as long-term storage of iron in ferritin. In the picophytoplanktonic species Ostreococcus we report evidence from observations both in the field and in laboratory cultures that ferritin and the main iron-binding proteins involved in photosynthesis and nitrate assimilation pathways show opposite diurnal expression patterns, with ferritin being maximally expressed during the night. Biochemical and physiological experiments using a ferritin knock-out line subsequently revealed that this protein plays a central role in the diel regulation of iron uptake and recycling and that this regulation of iron homeostasis is essential for cell survival under iron limitation.

Published
2015
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8. Distributions and stoichiometry of dissolved nitrogen and phosphorus in the iron-fertilized region near Kerguelen (Southern Ocean)

Author

Louise Oriol, Stéphane Blain, Ingrid Obernosterer, J. Capparos, Audrey Guéneuguès, Laboratoire d'Océanographie Microbienne (LOMIC), Observatoire océanologique de Banyuls (OOB), Université Pierre et Marie Curie - Paris 6 (UPMC)-Centre National de la Recherche Scientifique (CNRS)-Université Pierre et Marie Curie - Paris 6 (UPMC)-Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS), Centre National de la Recherche Scientifique (CNRS)-Université Pierre et Marie Curie - Paris 6 (UPMC)-Observatoire océanologique de Banyuls (OOB), and Université Pierre et Marie Curie - Paris 6 (UPMC)-Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS)

Subjects
0106 biological sciences, Pycnocline, 010504 meteorology & atmospheric sciences, lcsh:Life, chemistry.chemical_element, Mineralogy, [SDU.STU]Sciences of the Universe [physics]/Earth Sciences, 01 natural sciences, Nutrient, [SDU.STU.GC]Sciences of the Universe [physics]/Earth Sciences/Geochemistry, lcsh:QH540-549.5, TRACER, 14. Life underwater, Ecology, Evolution, Behavior and Systematics, 0105 earth and related environmental sciences, Earth-Surface Processes, Polar front, Remineralisation, geography, Plateau, geography.geographical_feature_category, 010604 marine biology & hydrobiology, Phosphorus, lcsh:QE1-996.5, Nitrogen, lcsh:Geology, lcsh:QH501-531, chemistry, 13. Climate action, Environmental chemistry, lcsh:Ecology
Abstract

During KEOPS2 (Kerguelen Ocean and Plateau Compared Study 2), we determined dissolved inorganic and organic nitrogen and phosphorus species in the naturally fertilized region of Kerguelen Island (Southern Ocean). Above 150 m, stations were clearly separated by the polar front (PF), with concentrations of NO3-, NO2- and PO43- overall lower north of the PF than south. Though less pronounced, a similar trend was detectable for dissolved organic nitrogen (DON) and dissolved organic phosphorus (DOP). At all stations offshore and above the plateau, a subsurface maximum of NH4+ was observed between 50 and 150 m. We examined nutrient stoichiometry by calculating the linear combination N* = [NO3-]-16 [PO43-]. The majority of stations and depths revealed N* close to −3 μM; however, for surface waters north of the PF, N* increased up to 6 μM. This suggests a preferential uptake of PO43- versus NO3- by fast-growing diatoms. Using the tracer TNxs = [TDN]-16[TDP] (TDN, total dissolved nitrogen; TDP, total dissolved phosphorus) revealed that the dissolved organic fraction significantly contributed to changes in TNxs. TNxs values were negative for most stations and depths, and relatively constant in the 0–500 m layer. As for N*, the stations north of the PF had higher TNxs in the 0–100 m layer. We discuss this stoichiometric anomaly with respect to possible external sources and sinks of N and P. Additional data collected in February 2013 at two sites revealed the occurrence of a subsurface minimum of N* located just below the pycnocline, which denotes a layer where remineralization of particulate organic matter with low N : P ratio P, possibly associated with preferential remineralization of P versus N, persists throughout the season.

Published
2015
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